• Journal of Life Science
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• v.33 no.5
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• pp.397-405
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• 2023

Although glutathione (GSH) has been shown to play an important role in the prevention of oxidative damage as an antioxidant, studies on immune regulation by it have not been properly conducted. In this study, we investigated whether luthione^®, a reduced GSH, has an immune enhancing effect in murine macrophage RAW 264.7 cells. The results of flow cytometry and immunofluorescence experiments indicated that luthione increased phagocytic activity, a representative function of macrophages, compared to the control cells. According to the results of the cytokine array, the expression of interleukin (IL)-5, IL-1β, and IL-27 was significantly increased in the luthione-treated cells. Luthione also enhanced the production of tumor necrosis factor-α and IL-1β through increased expression of their proteins, and increased release of the immune mediators such as nitric oxide (NO) and prostaglandin E₂ was associated with increased expression of inducible NO synthase and cyclooxygenase-2. In addition, the expression of cluster of differentiation 86, an M1 macrophage marker, was dramatically enhanced in RAW 264.7 cells treated with luthione. Furthermore, as a result of heat map analysis, we found that cytokine signaling 1/3-mediated signal transducer and activator of transcription/Janus tyrosine kinase signaling pathway was involved in the immunomodulatory effect by luthione. In conclusion, our data suggested that luthione could act as a molecular regulator in M1 macrophage polarization and enhance immune capacity by promoting macrophage phagocytic function.

• Journal of Life Science
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• v.33 no.8
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• pp.651-662
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• 2023

Peroxisomes, known as microbodies, are a class of morphologically similar subcellular organelles commonly found in most eukaryotic cells. They are 0.2~1.8 ㎛ in diameter and are bound by a single membrane. The matrix is usually finely granular, but occasionally crystalline or fibrillary inclusions are observed. They characteristically contain hydrogen peroxide (H₂O₂) generating oxidases and contain the enzyme catalase, thus confining the metabolism of the poisonous H₂O₂ within these organelles. Therefore, the eukaryotic organelles are greatly dynamic both in morphology and metabolism. Plant peroxisomes, in particular, are associated with numerous metabolic processes, including β-oxidation, the glyoxylate cycle and photorespiration. Furthermore, plant peroxisomes are involved in development, along with responses to stresses such as the synthesis of important phytohormones of auxins, salicylic acid and jasmonic acids. In the past few decades substantial progress has been made in the study of peroxisome biogenesis in eukaryotic organisms, mainly in animals and yeasts. Advancement of sophisticated techniques in molecular biology and widening of the range of genomic applications have led to the identification of most peroxisomal genes and proteins (peroxins, PEXs). Furthermore, recent applications of proteome study have produced fundamental information on biogenesis in plant peroxisomes, together with improving our understanding of peroxisomal protein targeting, regulation, and degradation. Nonetheless, despite this progress in peroxisome development, much remains to be explained about how peroxisomes originate from the endoplasmic reticulum (ER), then assemble and divide. Peroxisomes perform dynamic roles in many phases of plant development, and in this review, we focus on the latest progress in furthering our understanding of plant peroxisome functions, biogenesis, and dynamics.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• v.6 no.8
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• pp.63-72
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• 2016

The main purpose of this study was to empirically validate whether a factor in reducing youth externalizing problem behaviors impact analysis and affection between father and youth self-esteem externalizing problem behavior through effective regulation. The survey was conducted by the researcher who visits the school to collect the sample data by random sampling method on 336 male students at D area. After delating the 38 insincere questionnaires, final 298 data were analyzed. Using SPSS 21.0, the simple correlational analysis was conducted to decide the relationship among the variables and in order to know the reciprocal model, hierarchical multiple regression analysis was implemented. The results showed the esteem and the affection his father on a statistically significant effect on youth externalizing problem behavior, father attachment had the effect of regulating the relationship between self-esteem and externalizing problem behavior. Through these results through the self-esteem Improvement Plan of the Father and the love of young people and to promote a proposal for reducing externalizing problem behavior.

• 한국노년학
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• v.34 no.2
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• pp.247-258
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• 2014

The aim of this study was to investigate the influence of resilience and depression on suicidal ideation in elderly. The survey was conducted by 335 subjects (aged 65 to 70 years old; both gender) living in Daegu and Gyeongbuk district. Data were collected through interview-based questionnaires which were constructed to include the general characteristics(8 items), suicidal ideation(19 items), depression(15 items), and resilience(27 items). Collected data were analyzed by frequency, t-test, one-way ANOVA, and regression analysis. The present results showed that the main general factors affecting the resilience of elderly were age, the type of dwelling, pin money, and health. The influencing factors on suicidal ideation were health status, depression level, and the resilience in elderly. In particular, the suicidal ideation was more affected by self-regulation and affirmation of the subcategory in resilience. In conclusion, the resilience and depression is need to be considered as one of the main factors for decreasing the suicidal ideation in elderly.

• Korean Journal of Culture and Social Issue
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• v.14 no.1
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• pp.47-75
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• 2008

Research on moral judgment has been dominated by rational factor or moral emotion, in which moral judgment is thought to be caused by moral reasoning or moral intuitions. This research demonstrates that offender' anger can influence moral judgment. The study examined the role of offender's anger (control group versus anger group) on moral domain ( life, interpersonal ethic, traffic regulation: 6 case) : 2(groups) ×6(cases) mixed design. Participants were asked to judge the offender, case, sympathy and one's anger about the him or her who offended against the law or convention. Participants who perceived the offender's anger tended to assess questionnaire generous. In interpersonal ethic domain, participants have not been affected by anger. There was not significantly differences between two groups in interpersonal ethic domain. The results of experiment confirmed hypotheses that influence of anger varies with the moral domains affected a value system of culture. These findings indicate functional emotion for moral judgment and suggest people may be decide how much emotion is considered on moral judgment performance according to moral domains.

• Journal of Microbiology and Biotechnology
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• v.33 no.9
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• pp.1206-1212
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• 2023

The Wnt/β-catenin pathway plays essential roles in regulating various cellular behaviors, including proliferation, survival, and differentiation [1-3]. The intracellular β-catenin level, which is regulated by a proteasomal degradation pathway, is critical to Wnt/β-catenin pathway control [4]. Normally, casein kinase 1 (CK1) and glycogen synthase kinase-3β (GSK-3β), which form a complex with the scaffolding protein Axin and the tumor suppressor protein adenomatous polyposis coli (APC), phosphorylate β-catenin at Ser45, Thr41, Ser37, and Ser33 [5, 6]. Phosphorylated β-catenin is ubiquitinated by the β-transducin repeat-containing protein (β-TrCP), an F-box E3 ubiquitin ligase complex, and ubiquitinated β-catenin is degraded via a proteasome pathway [7, 8]. Colorectal cancer is a significant cause of cancer-related deaths worldwide. Abnormal up-regulation of the Wnt/β-catenin pathway is a major pathological event in intestinal epithelial cells during human colorectal cancer oncogenesis [9]. Genetic mutations in the APC gene are observed in familial adenomatous polyposis coli (FAP) and sporadic colorectal cancers [10]. In addition, mutations in the N-terminal phosphorylation motif of the β-catenin gene were found in patients with colorectal cancer [11]. These mutations cause β-catenin to accumulate in the nucleus, where it forms complexes with transcription factors of the T-cell factor/lymphocyte enhancer factor (TCF/LEF) family to stimulate the expression of β-catenin responsive genes, such as c-Myc and cyclin D1, which leads to colorectal tumorigenesis [12-14]. Therefore, downregulating β-catenin response transcription (CRT) is a potential strategy for preventing and treating colorectal cancer. Plant cytokinins are N⁶-substituted purine derivatives; they promote cell division in plants and regulate developmental pathways. Natural cytokinins are classified as isoprenoid (isopentenyladenine, zeatin, and dihydrozeatin), aromatic (benzyladenine, topolin, and methoxytopolin), or furfural (kinetin and kinetin riboside), depending on their structure [15, 16]. Kinetin riboside was identified in coconut water and is a naturally produced cytokinin that induces apoptosis and exhibits antiproliferative activity in several human cancer cell lines [17]. However, little attention has been paid to kinetin riboside's mode of action. In this study, we show that kinetin riboside exerts its cytotoxic activity against colon cancer cells by suppressing the Wnt/β-catenin pathway and promoting intracellular β-catenin degradation.

• Information Systems Review
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• v.24 no.2
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• pp.67-88
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• 2022

This study proposed a research model that can explain the usage intentions of users and non-users by considering the performance aspects of personal mobility and external environmental factors based on the SOR (Stimulus-Organism-Response) model, A survey was conducted targeting domestic users and non-users, and research models and hypotheses were verified through Partial Least Square (PLS) and Artificial Neural Network (ANN). As a result of the analysis, it was confirmed that the users' perceived satisfaction and perceived trust had a positive effect on their intention to use, and that perceived risk and environmental value had a significant relationship with perceived satisfaction and perceived trust. For non-users, it was found that there was a positive correlation between perceived satisfaction and intention to use, and it was verified that perceived risk and environmental value, like users, were significant antecedents of perceived satisfaction and perceived trust. Among the remaining variables, the perceived mobility of users and the perceived ease of use of non-users were respectively presented as important influencing factors on perceived satisfaction.

• Therapeutic Science for Rehabilitation
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• v.13 no.1
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• pp.65-74
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• 2024

Objective : This study assessed ChatGPT, an artificial intelligence system based on a large language model, for its ability to pass the National Korean Occupational Therapy Licensure Examination (NKOTLE). Methods : Using NKOTLE questions from 2018 to 2022, provided by the Korea Health and Medical Personnel Examination Institute, this study employed English prompts to determine the accuracy of ChatGPT in providing correct answers. Two researchers independently conducted the entire process, and the average accuracy of both researchers was used to determine whether ChatGPT passed over the 5-year period. The degree of agreement between ChatGPT answers of the two researchers was assessed. Results : ChatGPT passed the 2020 examination but failed to pass the other 4 years' examination. Specifically, its accuracy in questions related to medical regulations ranged from 25% to 57%, whereas its accuracy in other questions exceeded 60%. ChatGPT exhibited a strong agreement between researchers, except for medical regulation questions, and this agreement was significantly correlated with accuracy. Conclusion : There are still limitations to the application of ChatGPT to answer questions influenced by language or culture. Future studies should explore its potential as an educational tool for students majoring in occupational therapy through optimized prompts and continuous learning from the data.

• Journal of Life Science
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• v.34 no.5
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• pp.356-364
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• 2024

Lymph nodes (LNs) are crucial sites where immune responses are initiated to combat invading pathogens in the body. LNs are organized into distinctive compartments by stromal cells. Stromal cell subsets constitute special niches supporting the trafficking, activation, differentiation, and crosstalk of immune cells in LNs. Fibroblastic reticular cells (FRC) are a type of stromal cell that form the three-dimensional structure networks of the T cell-rich zones in LNs, providing guidance paths for immigrating T lymphocytes. FRCs imprint immune responses by supporting LN architecture, recruiting immune cells, coordinating immune cell crosstalk, and presenting antigens. During inflammation, FRCs exert both spatial and molecular regulation on immune cells through their topological and secretory responses, thereby steering immune responses. Here, we propose a model in which FRCs regulate immune responses through a three-part scheme: setting up, supporting, or suppressing immune responses. FRCs engage in bidirectional interactions that enhance T cell biological efficiency. In addition, FRCs have profound effects on the innate immune response through phagocytosis. Thus, FRCs in LNs act as gatekeepers of immune responses. Overall, this study aims to highlight the emerging roles of FRCs in controlling both innate and adaptive immunity. This collaborative feedback loop mediated by FRCs may help maintain tissue function during inflammatory responses.

• The Journal of the Convergence on Culture Technology
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• v.10 no.1
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• pp.71-79
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• 2024

ChatGPT, an interactive artificial intelligence (AI) chatbot developed by Open AI in the U.S., gaining popularity with great repercussions around the world. Some academia are concerned that ChatGPT can be used by students for plagiarism, but ChatGPT is also widely used in a positive direction, such as being used to write marketing phrases or website phrases. There is also an opinion that ChatGPT could be a new future for "search," and some analysts say that the focus should be on fostering rather than excessive regulation. This study analyzed consciousness about ChatGPT for college students through a survey of their perception of ChatGPT. And, plagiarism inspection systems were prepared to establish an education support model using ChatGPT and ChatGPT. Based on this, a university education support model using ChatGPT was constructed. The education model using ChatGPT established an education model based on text, digital, and art, and then composed of detailed strategies necessary for the era of the 4th industrial revolution below it. In addition, it was configured to guide students to use ChatGPT within the permitted range by using the ChatGPT detection function provided by the plagiarism inspection system, after the instructor of the class determined the allowable range of content generated by ChatGPT according to the learning goal. By linking and utilizing ChatGPT and the plagiarism inspection system in this way, it is expected to prevent situations in which ChatGPT's excellent ability is abused in education.