• Journal of Microbiology
• /
• v.42 no.2
• /
• pp.133-138
• /
• 2004

This study was aimed to determine the accumulation of free fatty acid by mesophilic lactic acid bac-teria (Lactococcus lactis subsp. lactis 1471, Lactococcus lactis subsp. cremoris 1000 and Lactobacillus casei 111) in cold-stored milk. According to the results, all cold-stored milks had higher acid degree val-ues than those of fresh milk. This phenomenon showed that a slight increase occurred in the accumulation of free fatty acids as a result of spontaneous lipolysis during cold storage. All lactic acid bacteria showed good performance in production of titratable acidity, which increased during fermentation of the milk (fresh and stored milks). Moreover, as the storage time was prolonged, more free fatty acid accumulation was obtained from the fermentation of the cold-stored milk by the investigated lactic acid bacteria. The control milk, which was without lactic acid bacteria, showed no change in the accumulation of free fatty acid during fermentation. From this result, it can be suggested that longer cold-storage time can induce higher free fatty acid accumulation in milk by lactic acid bacteria.

• Herbal Formula Science
• /
• v.26 no.4
• /
• pp.363-380
• /
• 2018

Objectives : We analyzed the principle of compatibility of socheongnyong-tang and expanded the range of application in clinical practice. Methods : Socheongnyong-tang was analyzed by the compatibility principle through the system of chief, deputy, assistant, and envoy, but the basic formulas of "Discussion of Cold Damage" and "Synopsis of the Golden Chamber" was used. Results : Socheongnyong-tang treats exterior wind-cold and interior water accumulation. Exterior wind-cold is treated mainly by exterior-releasing herbs constituting mahwang-tang and gyeji-tang in "Discussion of Cold Damage", and interior water accumulation is treated mainly by retained fluid-resolving herbs constituting yeonggamomigangsinha-tang, yeonggamomigagangsinbanhahaengin-tang and yeonggamomigagangsinbanhaengdaehwang-tang in "Synopsis of the Golden Chamber". Depending on the weight of exterior wind-cold and interior water accumulation, the disease condition is classified as a case where exterior wind-cold is heavier than interior water accumulation, a case where exterior wind-cold is lighter than interior water accumulation, and a case where it is equivalent. When exterior wind-cold is heavier than interior water accumulation, chief herbs are Ephedrae Herba and Cinnamomi Ramulus of exterior-releasing herbs series and deputy herbs are Zingiberis Rhizoma and Asari Radix et Rhizoma of retained fluid-resolving herbs series. When exterior wind-cold is equal to the disease condition of interior water accumulation, chief herbs are Ephedrae Herba of exterior-releasing herbs series and Zingiberis Rhizoma of retained fluid-resolving herbs series, and deputy herbs are Cinnamomi Ramulus of the exterior-releasing herbs series and Asari Radix et Rhizoma of retained fluid-resolving herbs series. When exterior wind-cold is lighter than interior water accumulation, chief herbs are Zingiberis Rhizoma and Asari Radix et Rhizoma of retained fluid-resolving herbs series and deputy herbs are Ephedrae Herba and Cinnamomi Ramulus of exterior-releasing herbs series. In any case, assistant herbs are Pinelliae Rhizoma, Paeoniae Radix alba, and Schisandrae Fructus, and envoy herb is Glycyrrhizae Radix preparata. Conclusions : In conclusion, socheongnyong-tang must differently formulate the system of chief, deputy, assistant, and envoy according to the grade of exterior wind-cold and interior water accumulation. These results suggest that socheongnyong-tang can be applied flexibly when applied in clinical practice to enhance the therapeutic effect.

• Journal of Ginseng Research
• /
• v.44 no.5
• /
• pp.747-755
• /
• 2020

Background: Ginsenosides accumulation responses to temperature are critical to quality formation in cold-dependent American ginseng. However, the studies on cold requirement mechanism relevant to ginsenosides have been limited in this species. Methods: Two experiments were carried out: one was a multivariate linear regression analysis between the ginsenosides accumulation and the environmental conditions of American ginseng from different sites of China and the other was a synchronous determination of ginsenosides accumulation, overall DNA methylation, and relative gene expression in different tissues during different developmental stages of American ginseng after experiencing different cold exposure duration treatments. Results: Results showed that the variation of the contents as well as the yields of total and individual ginsenosides Rg1, Re, and Rb1 in the roots were closely associated with environmental temperature conditions which implied that the cold environment plays a decisive role in the ginsenoside accumulation of American ginseng. Further results showed that there is a cyclically reversible dynamism between methylation and demethylation of DNA in the perennial American ginseng in response to temperature seasonality. And sufficient cold exposure duration in winter caused sufficient DNA demethylation in tender leaves in early spring and then accompanied the high expression of flowering gene PqFT in flowering stages and ginsenosides biosynthesis gene PqDDS in green berry stages successively, and finally, maximum ginsenosides accumulation occurred in the roots of American ginseng. Conclusion: We, therefore, hypothesized that cold-induced DNA methylation changes might regulate relative gene expression involving both plant development and plant secondary metabolites in such cold-dependent perennial plant species.

• Journal of Acupuncture Research
• /
• v.39 no.4
• /
• pp.267-274
• /
• 2022

In order to secure clinical evidence for the treatment of cold accumulation (CA), this study reviewed and analyzed 91 records of literature and related records. To perform a classical literature search, the Oriental Medicine Classic databases and the Uibujeonrok electronic data were searched. Books on Immunopharmacopuncture (IP) were also reviewed. A common etiology for CA was attributed to the abdomen, while the detailed sites of the abdomen differed. In IP, CA had a more comprehensive list of symptoms of physiological and pathological significance. As for the treatment of CA, typically, herbal medicine was used until the end of the Korean Empire period, and moxibustion treatment on CV4, CV6, CV-12, ST36, EX-B4, and conduction exercise therapy were also reported. Since 1965, acupuncture needle-based invasive treatment such as acupuncture for CA, and IP have been described in IP theory. Consequently, the treatment of CA (as described in the IP theory), was considered to be a more advanced method of treatment compared with the existing classical treatment methods.

• Journal of Haehwa Medicine
• /
• v.18 no.2
• /
• pp.119-129
• /
• 2009

The purpose of this study is to find out relationship between "Yakjing" and the textbook of herbology. Conclusions are as below. 1. The herbs of accumulation of poison(結毒) generally show cold nature and have a reducing effect, which resemble the method of treating accumulation and stagnation. 2. The herbs of muscular contracture(攣) show sweet taste and tonifying effect, fall on the spleen and the stomach and have acts of treating urgency, relieving pain and conciliating, which resemble the method of treating urgency(急)and pain from accumulation and stagnation. 3. The herbs of water poisons(水毒) are categorized as those of cold nature and dampness and we can find out that water poisons is related to cold nature. 4. The herbs of feverish feeling(煩) treat heat. 5. The herbs of cardiothoracic part have an effect of regulating Gi and treat the phlegm disease.

• Horticultural Science & Technology
• /
• v.32 no.5
• /
• pp.607-613
• /
• 2014

Calcium has been associated with improved cold tolerance in many crops. The aim of this study was to investigate the changes in leaf cell $Ca^{2+}$ distribution and cell organelle ultrastructure of loquat (Eriobotrya japonica Lindl.) plants in response to cold stress at $-3^{\circ}C$, using transmission electron microscopy (TEM). Two loquat accessions, Zaozhong 6 (a commercial cultivar) and oakleaf loquat (a wild relative) were used. Cold tolerance, as measured by leaf browning rate, was higher in oakleaf plants, and calcium treatment improved cold tolerance in both species. Cold stress first induced inward transport of $Ca^{2+}$ from the intracellular space. Then, the imported $Ca^{2+}$ was aggregated around the chloroplast membrane, finally entering the chloroplast. This pattern of $Ca^{2+}$ distribution in leaf cells occurred earlier in Zaozhong 6 than in the wild loquat. With increasing time of cold exposure, the chloroplast membranes of Zaozhong 6 leaves were damaged, blurred and even disappeared, while those of wild oakleaf loquat leaves maintained their structure longer. In Zaozhong 6, cold stress induced a clear cavity between poorly structured granal thylakoids and vesicles appearing inside the chloroplast, while in oakleaf leaves cold stress had little effect on the ultrastructure of chloroplasts (although chloroplast membranes looked blurred). Loquat leaves accumulated free calcium ions around chloroplasts in response to cold stress, with earlier calcium accumulation occurring in the cold-sensitive cultivar Zaozhong 6 than in wild oakleaf loquat. These results demonstrate that these two loquat species have differences in both cold tolerance and calcium accumulation dynamics.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.5 no.4
• /
• pp.261-265
• /
• 2003

Cold air accumulation plays a critical role in formulating daily minimum temperature in complex terrain on radiative cooling nights, and spatial interpolation can be improved by accommodating this important topoclimatic variable. Little is known about the spatial scale for computing cold air accumulation which influences daily minimum temperature. Air temperature was measured at 10-minute intervals during September 2002- February 2003 at eight locations within a 1 by 1 km hilly orchard area. Minimum temperature data for suspected radiative cooling nights were collected, and the deviations from reference observations at a near-by KMA automated weather station were calculated. A digital elevation model with a 10m cell size was used to calculate the cold air accumulation at 8 locations. Zonal averages of the cold air accumulation were computed for each location by increasing the cell radius from 1 to 10. Temperature deviations were regressed to a common logarithm of the smoothed averages of cold air accumulation to derive a linear relationship between the local temperature deviation and the site topography. The highest coefficient of determination ($r^2$ = 0.78) was found at a cell radius of 5, which corresponds to an approximately 1 ha boundary surrounding the point of interest.

• 한국생물공학회:학술대회논문집
• /
• 2003.10a
• /
• pp.730-735
• /
• 2003

The effect of pathogenicity on trehalose accumulation was studied using six species of entomopathogenic nematodes adapted to either cold or warm temperature conditions. Also, trehalose accumulation was studied by treating nematodes with glycerol or oil at trehalose concentration and mortality of cold temperature $condition(5^{\circ}C)$, S. longicaudum Gl was increased 50% than control. And at trehalose concent and mortality of warm temperature $condition(30^{\circ}C)$, S. glaseri Agr B3 was increased 45%. Intracellular trehalose accumulation was increased with treatment with 20% glycerol or oil. As the final results, the pathogenic duration of nematodes was increased as intracellular trehalose accumulation was increased

• Journal of Plant Biology
• /
• v.14 no.1
• /
• pp.5-13
• /
• 1971

Accumulation of soil organic matter and its vertical distribution at different latitudes in peninsular Korea were studied in the soil of four different forest types viz. Pinus densiflora forest, Castanea forest, Quercus acutissima forest and Carpinus laxiflora forest. Among them, accumulation of soil organic matter in Cheju sites, with a mean annual temperature of 15$^{\circ}C$, was maximum with increasing latitude, soil organic matter concentration decreased. Considering the relationship between concentration of soil organic matter and some climatic conditiions, it seems that concentrations of soil organic matter is a function of annual temperature, especially warmth index or cold index.

• Applied Biological Chemistry
• /
• v.34 no.2
• /
• pp.162-167
• /
• 1991

In an attempt to explore the mechanistic aspects of chilling injury in plants and their defensive measures against the low temperature stress, the time sequential measurements of pyruvate, superoxide radicals$(O_{\overline{2}})$ and antioxygenic enzymes during whole period of injury-inducing treatment were performed using mostly rice seedlings. Pyruvate was substantialy accumulated in leaf tissues during the exposure period to $5^{\circ}C$ of the seedlings ; the relative extent of the accumulation was increased with increasing time of the cold treatment. When the cold-treated plants were translocated to ambient temperature$({\sim}25^{\circ}C)$, the accumulation started to dissipate, concomitantly accompaning a remarkable increase in the $O_{\overline{2}}$ level of tissues. Superoxide dismutase(SOD) and catalase were also activated during post-chilling period, although they showed a considerable lag time for activation. In contrast, glutathione peroxidase, another antioxygenic enzyme in cells, was not activated at all by preceding cold treatment of plants. The uptake of exogenous $O_{\overline{2}}$ by the roots of rice seedlings resulted in increase in the activities of SOD and catalase in root tissues. The supply of $H_2O_2$ to plan st brought about the activation of catalase in situ, while failing to exert any effect on the activation state of glutathione peroxidase. The results obtained in this work suggest that pyruvate accumulation in cells is the direct cause of the overproduction of $O_{\overline{2}}$ and thereby other toxic activated oxygen species, and that SOD and catalase may play a crucial role in the protection of plant cells against active oxygen-mediated chilling injury.