• Bulletin of the Korean Chemical Society
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• v.33 no.1
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• pp.105-110
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• 2012

A europium (III)-sensitized, spectrofluorimetric (FL) method is presented for the determination of sparfloxacin (SPAR) using an anionic surfactant, sodium dodecyl benzene sulphonate (SDBS). The method is based on the strong fluorescence (FL) enhancement of SPAR after the addition of $Eu^{3+}$ ions as fluorescence probes. The experimental results indicated that the FL intensity of the SPAR-$Eu^{3+}$ system was enhanced markedly by SDBS. The maximum FL emission signal was obtained at about 615 nm when excited at 372 nm. The experimental conditions that affected the FL intensity of the SPAR-$Eu^{3+}$-SDBS system were optimized systematically. The enhanced FL intensity of the system exhibited a good linear relationship with the SPAR concentration over the range of $1.5{\times}10^{-9}-1.2{\times}10^{-7}mol\;L^{-1}$ with a correlation coefficient (r) of 0.9987. The limit of detection ($3{\delta}$) was $4.15{\times}10^{-10}mol\;L^{-1}$ with a relative standard deviation (RSD) of 1.65%. This method was successfully applied for the determination of SPAR in pharmaceuticals, and human serum and urine samples with higher sensitivity, wide dynamic range and better stability. The possible interaction mechanism of the system is also discussed in detail by ultraviolet absorption spectra and FL spectra.

• Journal of Environmental Science International
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• v.24 no.4
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• pp.371-381
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• 2015

In the mountain streams in Jeju Island, strong turbulence and roughness usually made it nearly impossible to utilize most of intrusive instrumentation for streamflow discharge measurements. Instead, a non-intrusive fixed electro-magnetic wave surface velocimetry (fixed EWSV: Kalesto) became alternatively popular in many representative streams to measure stream discharge seamlessly. Currently, Kalesto has shown noteworthy performance with little loss in flood discharge measurements and also has successfully provided discharge for every minute. However, Kalesto has been operated to regard its measured one-point velocity as the representative mean velocity for the given cross-section. Therefore, it could be highly possible to potentially encompass discharge measurements errors. In this study, we analyzed the difference between such Kalesto discharge measurements and other alternative concurrent discharge measurements such as Acoustic Doppler Current Profiler (ADCP) and mobile EWSV which were able to measure velocity in multi-points in the cross-section. Consequently, Kalesto discharge deviated from ADCP discharge in amount of 48% for relatively low flow, and more than 20% difference for high flow compared with mobile EWSV discharge measurements. These results indicated that the one-point velocity measured by Kalesto should be used as a cross-sectional mean velocity, rather it should be accounted for as an index-velocity in conjunction with directly measured cross-sectional mean velocity by using more reliable instrumentations. After inducing Kalesto Discharge Correction Coefficient (KDCC) that actually means relationship between index velocity and cross-sectional mean velocity, the corrected discharge from Kalesto was significantly improved. Therefore, we found that index velocity method should be applied to obtain better accuracy of discharge measurement in case of Kalesto operation.

• Journal of Ocean Engineering and Technology
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• v.29 no.6
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• pp.436-444
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• 2015

A submerged body is sensitive to changes in the roll moment because of the small restoring moment and moment of inertia. Thus, a method for predicting the roll-related hydrodynamic coefficients is important. This paper describes a deduction method for the hydrodynamic coefficients based on the results of a coning motion test. A resistance test, static drift test, and coning motion test were performed to obtain the coefficients in the towing tank of Seoul National University. The sum of the hydrodynamic force, inertial force, gravity, and buoyancy was measured in the coning motion test. The hydrodynamic force was deduced by subtracting the inertial force, gravity, and buoyancy from the measured force. The hydrodynamic coefficients were deduced using the regression method.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.9
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• pp.2205-2212
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• 2015

The aim of this study was to explore the acceptance factors of IPTV healthcare users as convergence service through experts's opinion. First, this study extracted expected indicators for the acceptance factors from brainstorming and literature review. Based on the expected indicators, the Delphi method was performed in order to explore the suitable acceptance factors. The reliability of collected data was evaluated through the criteria of CV(Coefficient of Variation) and CVR(Contents Validity Ratio) on the selected indicators. The results showed a significant acceptance factors timeliness, the following appeared as entertainment, fun and self-efficacy and more. In the future, such additional step as factor analysis targeting the user to verify the validity of the selected factors is required.

• Transactions of the Korean Society of Automotive Engineers
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• v.24 no.1
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• pp.33-38
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• 2016

The turbocharger is an essential component to realize the engine down-sizing. The moment of inertia of turbocharger rotor is an important parameter with respect to acceleration performance of the vehicle. It can be calculated from the CAD software based the geometry data and the material properties. But the accurate value of the inertia of turbocharger rotor must be measured through the experimental method. In this study, the measurement of moment of inertia of turbocharger rotor for 2.0 L spark-ignition engine was carried out. First, an experimental equipment using a trifilar method was designed and fabricated. Some optical devices, that is, photo sensor, counter, convex lens, etc, were used to increase the accuracy of the measurement. Second, error sensitivity for the equipment was analyzed. The error of period time and the radius can give big affects to the accuracy of the moment of inertia. When the amount of error of these two were each 1.0 %, maximum error of the moment of inertia was under 3.0 %. Third, the calibration for the equipment was performed using a calibration rotor which has similar shape to turbine rotor but simple. Calculated value from CAD software and measured one for the calibration rotor were compared. The total error of the equipment and the measurement is about 1.3 %. This result shows that the equipment can give the good result with resonable accuracy. Finally the moment of inertia of the turbine rotor and compressor wheel were measured. The coefficient of variations, the ratio of standard deviation to mean value, were reasonably small at 0.57 % and 0.73 % respectively. Therefore this equipment is suitable for the measurement of the moment of inertia of the turbine rotor and compressor wheel.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.1
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• pp.387-392
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• 2020

This paper proposes a flow-rate control system for industrial valves. Industrial valves are used in piping systems to control the flow rate and pressure. In general, valves used in pipelines are classified into globe valves, butterfly valves, and ball valves according to the shape. Motor, hydraulic, and pneumatic systems are used for operating valves. The flow meter should measure the flow rate when configuring the flow-rate control system. On the other hand, because the flow rate of the valve can be expressed by flow coefficient, a control scheme is proposed using the pressure deviation, which measures at the front and rear of the valve. The transfer function for the valve, according to the control input, was estimated using the signal compression method. Based on the induced transfer function, the disturbance observer was designed to improve the command following the performance of the valve stem. The performance of the proposed control method is compared with the flow-rate control result using the flow meter used.

• Journal of Korea Water Resources Association
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• v.32 no.6
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• pp.607-616
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• 1999

A fractional step finite difference model for the longitudinal dispersion of nonconservative contaminants is developed. It is based on splitting the longitudinal dispersion equation into a set of three equations each to be solved over a one-third time step. The fourth-order Holly-Preissmann scheme, an analytic solution, and the Crank-Nicholson scheme are used to solve the equations for the pure advection, the first-order decay, and the diffusion, respectively. To test the model, it is applied to simulate the longitudinal dispersion of continuous source released into a nonuniform flow field as well as the dispersion of an instantaneous source in a uniform flow field. The results are compared with the exact solution and those computed by an existing model. Compared to the existing model which uses Euler method for the first-order decay equation, the present model yield more accurate results as the decay coefficient increases.

• Korean Journal of Agricultural Science
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• v.46 no.1
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• pp.183-194
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• 2019

Adulteration of argan oil with some other cheaper oils with similar chemical compositions has resulted in increasing demands for authenticity assurance and quality control. Fast and simple analytical techniques are thus needed for authenticity analysis of high-priced argan oil. Raman spectroscopy is a potent technique and has been extensively used for quality control and safety determination for food products In this study, Raman spectroscopy in combination with a net analyte signal (NAS)-based methodology, i.e., hybrid linear analysis method developed by Goicoechea and Olivieri in 1999 (HLA/GO), was used to predict the different concentrations of olive oil (0 - 20%) added to argan oil. Raman spectra of 90 samples were collected in a spectral range of $400-400cm^{-1}$, and calibration and validation sets were designed to evaluate the performance of the multivariate method. The results revealed a high coefficient of determination ($R^2$) value of 0.98 and a low root-mean-square error (RMSE) value of 0.41% for the calibration set, and an $R^2$ of 0.97 and RMSE of 0.36% for the validation set. Additionally, the figures of merit such as sensitivity, selectivity, limit of detection, and limit of quantification were used for further validation. The high $R^2$ and low RMSE values validate the detection ability and accuracy of the developed method and demonstrate its potential for quantitative determination of oil adulteration.

• Journal of the Korean Recycled Construction Resources Institute
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• v.7 no.1
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• pp.1-7
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• 2019

Recently, studies on alkali activated slag(AAS) binders that do not use cement have been actively conducted. It is known that AAS concrete is highly resistant to chloride damage based on the test method used for ordinary concrete. However, it is fully not understood whether the test method used for concrete can be applied to AAS mixtures. Therefore, in this study, we verified the consistency of NT Build 492 and ASTM C 1202 test methods by applying various experimental variables. According to the experimental results, the two tests yielded opposite results. Therefore, the chloride durability of AAS mortar can be different depending on the evaluation method.

• Biomolecules & Therapeutics
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• v.27 no.2
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• pp.193-200
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• 2019

Ceramide metabolism is known to be an essential etiology for various diseases, such as atopic dermatitis and Gaucher disease. Glucosylceramide synthase (GCS) is a key enzyme for the synthesis of glucosylceramide (GlcCer), which is a main ceramide metabolism pathway in mammalian cells. In this article, we developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to determine GCS activity using synthetic non-natural sphingolipid C8-ceramide as a substrate. The reaction products, C8-GlcCer for GCS, could be separated on a C18 column by reverse-phase high-performance liquid chromatography (HPLC). Quantification was conducted using the multiple reaction monitoring (MRM) mode to monitor the precursor-to-product ion transitions of m/z $588.6{\rightarrow}264.4$ for C8-GlcCer at positive ionization mode. The calibration curve was established over the range of 0.625-160 ng/mL, and the correlation coefficient was larger than 0.999. This method was successfully applied to detect GCS in the human hepatocellular carcinoma cell line (HepG2 cells) and mouse peripheral blood mononuclear cells. We also evaluated the inhibition degree of a known GCS inhibitor 1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP) on GCS enzymatic activity and proved that this method could be successfully applied to GCS inhibitor screening of preventive and therapeutic drugs for ceramide metabolism diseases, such as atopic dermatitis and Gaucher disease.