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Sequence Analysis of the Gene Encoding gp55 Protein of Suri Strain, an Attenuated Classical Swine Fever (Hog Cholera) Virus (Classical Swine Fever (Hog Cholera) Virus 약독순화주 (Suri 주)의 gp55 Gnen 염기서열 분석)

  • Kim, Kui-Hyun;Chang, Kyung-Soo;Kang, Kyong-Im;Lee, Byung-Hyung;Park, Jong-Hyeon;An, Soo-Hwan;Jun, Moo-Hyung
    • The Journal of Korean Society of Virology
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    • v.28 no.4
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    • pp.303-316
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    • 1998
  • An attenuated classical swine fever virus (CSFV), Suri strain, is a variant derived from a vaccine virus, LOM strain. This study was performed to elucidate the molecular biologcal properties of CSFV Suri strain, and to obtain the basic data for molecular epidemiological approaches for the disease. The truncated form of gp55 gene without the C-terminal transmembrane domain, in size of 1,023bp, was amplified by RT-PCR and sequenced by dye terminator cyclic sequencing method, and inserted into BamHI site of pAcGP67B baculovirus vector, establishing a cloned pAcHEG plasmid. By the nucleotide sequences determined, 341 amino acid sequences were predicted. As compared the nucleotide and amino acid sequences of gp55 of Suri with the various CSFV, Suri strain showed the high homology over 99.1% with ALD and LOM strains, but comparably the lower homology with Alfort and Brescia. In comparison of amino acid sequence in variable domain of gp55 protein, the similar tendency of homology was observed. In hydrophobicity analysis, all of four CSFV strains revealed the analogous patterns of hydrophobicity. The numbers and locations of N-glycosylation site and cysteine residues in gp55 were analyzed, those of Suri strain being coincident with ALD and LOM strains. The results suggest that gp55 in Suri strain has the high similarity to those in ALD and LOM strains in terms of the nucleotide and amino acid sequences and the functional properties of gp55 protein.

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Bending behavior of squared cutout nanobeams incorporating surface stress effects

  • Eltaher, Mohamed A;Abdelrahman, Alaa A.
    • Steel and Composite Structures
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    • v.36 no.2
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    • pp.143-161
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    • 2020
  • In nanosized structures as the surface area to the bulk volume ratio increases the classical continuum mechanics approaches fails to investigate the mechanical behavior of such structures. In perforated nanobeam structures, more decrease in the bulk volume is obtained due to perforation process thus nonclassical continuum approaches should be employed for reliable investigation of the mechanical behavior these structures. This article introduces an analytical methodology to investigate the size dependent, surface energy, and perforation impacts on the nonclassical bending behavior of regularly squared cutout nanobeam structures for the first time. To do this, geometrical model for both bulk and surface characteristics is developed for regularly squared perforated nanobeams. Based on the proposed geometrical model, the nonclassical Gurtin-Murdoch surface elasticity model is adopted and modified to incorporate the surface energy effects in perforated nanobeams. To investigate the effect of shear deformation associated with cutout process, both Euler-Bernoulli and Timoshenko beams theories are developed. Mathematical model for perforated nanobeam structure including surface energy effects are derived in comprehensive procedure and nonclassical boundary conditions are presented. Closed forms for the nonclassical bending and rotational displacements are derived for both theories considering all classical and nonclassical kinematics and kinetics boundary conditions. Additionally, both uniformly distributed and concentrated loads are considered. The developed methodology is verified and compared with the available results and an excellent agreement is noticed. Both classical and nonclassical bending profiles for both thin and thick perforated nanobeams are investigated. Numerical results are obtained to illustrate effects of beam filling ratio, the number of hole rows through the cross section, surface material characteristics, beam slenderness ratio as well as the boundary and loading conditions on the non-classical bending behavior of perforated nanobeams in the presence of surface effects. It is found that, the surface residual stress has more significant effect on the bending deflection compared with the corresponding effect of the surface elasticity, Es. The obtained results are supportive for the design, analysis and manufacturing of perforated nanobeams.

An Efficient Multidimensional Scaling Method based on CUDA and Divide-and-Conquer (CUDA 및 분할-정복 기반의 효율적인 다차원 척도법)

  • Park, Sung-In;Hwang, Kyu-Baek
    • Journal of KIISE:Computing Practices and Letters
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    • v.16 no.4
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    • pp.427-431
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    • 2010
  • Multidimensional scaling (MDS) is a widely used method for dimensionality reduction, of which purpose is to represent high-dimensional data in a low-dimensional space while preserving distances among objects as much as possible. MDS has mainly been applied to data visualization and feature selection. Among various MDS methods, the classical MDS is not readily applicable to data which has large numbers of objects, on normal desktop computers due to its computational complexity. More precisely, it needs to solve eigenpair problems on dissimilarity matrices based on Euclidean distance. Thus, running time and required memory of the classical MDS highly increase as n (the number of objects) grows up, restricting its use in large-scale domains. In this paper, we propose an efficient approximation algorithm for the classical MDS based on divide-and-conquer and CUDA. Through a set of experiments, we show that our approach is highly efficient and effective for analysis and visualization of data consisting of several thousands of objects.

A Study of the Influence of Medium Richness and Learner's Experience with Various Mediums on the Usefulness of Mediums and Learning Commitment in Integrated Media Korean Classical Education (매체통합 고전문학수업에서 매체풍부성과 매체경험이 매체유용성과 학습몰입에 미치는 영향 연구)

  • Hyun, Young-Ran;Chung, So-Yeon
    • Journal of Korean Library and Information Science Society
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    • v.46 no.4
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    • pp.471-491
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    • 2015
  • The purpose of this paper is to examine the structural relationship between the use of a web medium and learning commitment to develop creative talent for higher media integrated Korean classical literature education. For this we used DBs of The 'Encyclopedia of Korean Local Culture(a local culture DB)' built by the Academy of Korean Studies, and a survey was conducted on 418 high school students, attending a classical literature class which used a local culture DB. The result of this study demonstrates media usefulness of local culture DBs' positive effect on learning commitment. Specifically, media richness and media experience affects the learning commitment through the medium usefulness. These results indicate that in order to encourage learner's medium experience and increase medium richness it is necessary to increase the utilization of mediums, such as local culture DBs.

Excitonic Energy Transfer of Cryptophyte Phycocyanin 645 Complex in Physiological Temperature by Reduced Hierarchical Equation of Motion

  • Lee, Weon-Gyu;Rhee, Young Min
    • Bulletin of the Korean Chemical Society
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    • v.35 no.3
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    • pp.858-864
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    • 2014
  • Recently, many researches have shown that even photosynthetic light-harvesting pigment-protein complexes can have quantum coherence in their excitonic energy transfer at cryogenic and physiological temperatures. Because the protein supplies such noisy environment around pigments that conventional wisdom expects very short lived quantum coherence, elucidating the mechanism and searching for an applicability of the coherence have become an interesting topic in both experiment and theory. We have previously studied the quantum coherence of a phycocyanin 645 complex in a marine algae harvesting light system, using Poisson mapping bracket equation (PBME). PBME is one of the applicable methods for solving quantum-classical Liouville equation, for following the dynamics of such pigment-protein complexes. However, it may suffer from many defects mostly from mapping quantum degrees of freedom into classical ones. To make improvements against such defects, benchmarking targets with more accurately described dynamics is highly needed. Here, we fall back to reduced hierarchical equation of motion (HEOM), for such a purpose. Even though HEOM is known to applicable only to simplified system that is coupled to a set of harmonic oscillators, it can provide ultimate accuracy within the regime of quantum-classical description, thus providing perfect benchmark targets for certain systems. We compare the evolution of the density matrix of pigment excited states by HEOM against the PBME results at physiological temperature, and observe more sophisticated changes of density matrix elements from HEOM. In PBME, the population of states with intermediate energies display only monotonically increasing behaviors. Most importantly, PBME suffers a serious issue of wrong population in the long time limit, likely generated by the zero-point energy leaking problem. Future prospects for developments are briefly discussed as a concluding remark.

Applicability Evaluation of IGM시s Theory Using the Results of Load Transfer Tests of Drilled Shafts (현장타설말뚝의 하중전이시험 결과를 이용한 IGM 이론의 적용성 평가)

  • 천병식;김원철;서덕동;윤우현
    • Journal of the Korean Geotechnical Society
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    • v.20 no.6
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    • pp.29-40
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    • 2004
  • The bearing capacity of drilled shaft is affected by several factors, such as shaft length, shape, surface roughness, young's modulus of geomaterials and shaft, soil strength, confining stress and so on. However, there has been no design method of drilled shaft considering all factors mentioned above. Moreover, since geomaterials are simply classified as sand, clay and rock, there was no design criterion for IGM (Intermediate Geomaterials). Therefore, the rigorous design approach of drilled shaft was not possible by classical design method. However, since these characteristics were not considered in classical theories, bearing capacity was generally different ken practical value. In this study, the bearing capacity of drilled shaft with the IGM's theory was compared with those of classical theories. The results showed that classical method showed smaller values of bearing capacity than those of field load transfer data. Moreover, the evaluated value of bearing capacity with IGM theory corresponded fairly well with those of field data.

Comparative Analysis of Morphometric Characteristics of Scorpaenidae and Gobioninae

  • Park, In-Seok;Gil, Hyun Woo;Oh, Ji Su;Choi, Hui Jung;Kim, Chi Hong
    • Development and Reproduction
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    • v.19 no.2
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    • pp.85-96
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    • 2015
  • Measurements of closely related sets of classical and truss dimensions were analyzed to discriminate species of scorpaenidae including the dark banded rockfish, Sebastes inermis, the black rockfish, S. schlegeli, and gobioninae including the striped shiner, Pungtungia herzi, and the slender shiner, Pseudopungtungia tenuicorpa. The measurements of the dimensions were arc sin square root transformed, and compared as a function of the standard length of each species for statistical analysis. For values of the classical dimensions of the rockfish, 6 were greater for the dark banded rockfish than for the black rockfish, 1 value was smaller for the former, and for 2 values there was no statistically significant difference (P > 0.05). For values of the classical dimensions of the shiners, 9 values were greater for the striped shiner than for the slender shiner, 2 values were smaller for the former, and for 1 value there was no statistically significant difference (P > 0.01). For values of the truss dimensions of the rockfish, 6 were greater for the dark banded rockfish than for the black rockfish, 1 was smaller for the former, and for 4 values there was no statistically significant difference (P > 0.05). For values of the truss dimensions of the shiners, 13 values were greater for the striped shiner than for the slender shiner, 3 values were smaller for the former, and for 6 values there was no statistically significant difference (P > 0.01). The dimension sets used in this study may be useful as taxonomic indicators for discriminating among fish species in Korea.

Force-based Coupling of Peridynamics and Classical Elasticity Models (페리다이나믹과 탄성체 모델의 연성기법 개발)

  • Ha, Youn Doh;Byun, Taeuk;Cho, Seonho
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.27 no.2
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    • pp.87-94
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    • 2014
  • In solid mechanics, the peridynamics theory has provided a suitable framework for material failure and damage propagation simulation. Peridynamics is computationally expensive since it is required to solve enormous nonlocal interactions based upon integro-differential equations. Thus, multiscale coupling methods with other local models are of interest for efficient and accurate implementations of peridynamics. In this study, peridynamic models are restricted to regions where discontinuities or stress concentrations are present. In the domains characterized by smooth displacements, classical local models can be employed. We introduce a recently developed blending scheme to concurrently couple bond-based peridynamic models and the Navier equation of classical elasticity. We demonstrate numerically that the proposed blended model is suitable for point loads and static fracture, suggesting an alternative framework for cases where peridynamic models are too expensive, while classical local models are not accurate enough.

A Bibliographic Study on Low Back Pain and Related General Symptoms in Classical Literatures - Standardization for Classification and Diagnosis of Low Back Pain - (고전에 나타난 요통 및 관련 전신 증상에 관한 문헌적 고찰 - 한의학적 분류 및 진단 체계의 표준화를 위한 기초 자료 수집을 중심으로 -)

  • Kwak, Hyun-Young;Nam, Dong-Woo;Kang, Jung-Won;Kim, Eun-Jung;Kim, Kap-Sung;Choi, Do-Young;Lee, Jae-Dong
    • Journal of Acupuncture Research
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    • v.27 no.1
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    • pp.31-41
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    • 2010
  • Objectives : The purpose of this study is to set up the standard for Classification and Diagnosis of Low Back Pain by through collecting bibliographic study on Low Back Pain and related general symptoms in classical literatures Methods : We investigated the contents of classical literatures about chronic low back pain and related general symptoms. With this contents, we established a systemic classification and diagnostic standard for Low back pain. Results : There are many different opinions on classification of low back pain and general symptoms in oriental medicine classical literature. Every opinion is reasonable, so it is difficult to establish a diagnosis of Low back pain. But it is necessary to set up the one-systemic classification and diagnostic technique of Low back pain. Conclusions : We conclude that the Ten type Low back pain classification of in Dong-Eui-Bo-Gam is a reasonable standard for diagnostic classification.

The research of classical music performance with the techniques borrowed from pop-style arrangements (In rhythm concentrated) (대중음악 편곡 기법을 활용한 클래식곡의 연주에 관한 연구 (리듬섹션을 중심으로))

  • Lee, Tae-Yoon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.9
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    • pp.458-463
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    • 2016
  • New areas can be created by combining separate fields of study. Today, boundaries between fields are collapsing because of rapid progress in technologies, which is also the case in music. In 1969, jazz trumpeter and respected artist Miles Davis combined jazz, rock, and classical music into the fusion genre. Classical music is known to be strict, rigorous, and rigid, and it started to take a different form after experiments with musical crossovers. This study examines the potential of more diverse musical crossovers that musicians use to evolve a new genre. Several musicians in Korea have attempted musical crossovers by following global music trends. In the album, "Love and Peace," the classical music pieces "Flight of the Bumblebee" and Beethoven's "Fate" were arranged using a popular music style.