• Archives of Pharmacal Research
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• 제26권1호
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• pp.28-33
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• 2003

Ginsenosides, major active ingredients of Panax ginseng, are known to regulate excitatory ligand-gated ion channel activity such as nicotinic acetylcholine and NMDA receptor channel activity. However, it is not known whether ginsenosides affect inhibitory ligand-gated ion channel activity. We investigated the effect of ginsenosides on human recombinant $GABA_A$ receptor (${\alpha}_1{\beta}_1{\gamma}_{2s}$) channel activity expressed in Xenopus oocytes using a two-electrode voltage-clamp technique. Among the eight individual ginsenosides examined, namely, $Rb_1$, $Rb_2$, Rc, Rd, Re, Rf, $Rg_1$ and $Rg_2$, we found that Rc most potently enhanced the GABA-induced inward peak current ($I_{GABA}$). Ginsenoside Rc alone induced an inward membrane current in certain batches of oocytes expressing the $GABA_A$ receptor. The effect of ginsenoside Rc on $I_{GABA}$ was both dose-dependent and reversible. The half-stimulatory concentration ($EC_{50}$) of ginsenoside Rc was 53.2$\pm$12.3 $\mu$M. Both bicuculline, a $GABA_A$ receptor antagonist, and picrotoxin, a $GABA_A$ channel blocker, blocked the stimulatory effect of ginsenoside Rc on $I_{GABA}$. Niflumic acid (NFA) and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), both $CI^{-1}$ channel blockers, attenuated the effect of ginsenoside Rc on I$I_{GABA}$. This study suggests that ginsenosides regulated $GABA_A$ receptor expressed in Xenopus oocytes and implies that this regulation might be one of the pharmacological actions of Panax ginseng.

• 대한전자공학회논문지SD
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• 제48권12호
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• pp.31-36
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• 2011

본 논문은 0.13um CMOS 공정에서 적층출력드라이버 ESD 내성에 대하여 조사 하였다. 실제적인 I/O 시스템과 유사하게 프리-드라이버와 파워 클램프를 포함한 적층출력드라이버 회로를 구현하였다. 프리-드라이버 입력 연결 방법과 적층출력드라이버의 NMOS 크기에 따라 8가지 회로를 구성하였으며, TLP 실험을 통해서 HBM 내성을 조사하였다. 그 결과 프리-드라이버의 입력에 전원전압을 인가하고 적층출력드라이버는 가급적 유사한 크기로 진행한 조건이 다른 조건들 보다 높은 항복전류와 항복전압을 보여주었다. 이 테스트 결과를 토대로, 적층출력드라이버의 ESD 내성을 향상시킬 수 있는 설계 가이드를 제안하였다.

• International Journal of Oral Biology
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• 제36권2호
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• pp.83-89
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• 2011

Substantia gelatinosa (SG) neurons receive synaptic inputs from primary afferent $A{\delta}$- and C-fibers, where nociceptive information is integrated and modulated by numerous neurotransmitters or neuromodulators. A number of studies were dedicated to the molecular mechanism underlying the modulation of excitability or synaptic plasticity in SG neurons and revealed that second messengers, such as cAMP and cGMP, play an important role. Recently, cAMP and cGMP were shown to downregulate each other in heart muscle cells. However, involvement of the crosstalk between cAMP and cGMP in neurons is yet to be addressed. Therefore, we investigated whether interaction between cAMP and cGMP modulates synaptic plasticity in SG neurons using slice patchclamp recording from rats. Synaptic activity was measured by excitatory post-synaptic currents (EPSCs) elicited by stimulation onto dorsal root entry zone. Application of 1 mM of 8-bromoadenosine 3,5-cyclic monophosphate (8-Br-cAMP) or 8-bromoguanosine 3,5-cyclic monophosphate (8-Br-cGMP) for 15 minutes increased EPSCs, which were maintained for 30 minutes. However, simultaneous application of 8-BrcAMP and 8-Br-cGMP failed to increase EPSCs, which suggested antagonistic cross-talk between two second messengers. Application of 3-isobutyl-1-methylxanthine (IBMX) that prevents degradation of cAMP and cGMP by blocking phosphodiesterase (PDE) increased EPSCs. Co-application of cAMP/cGMP along with IBMX induced additional increase in EPSCs. These results suggest that second messengers, cAMP and cGMP, might contribute to development of chronic pain through the mutual regulation of the signal transduction.

• 대한기계학회논문집A
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• 제39권11호
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• pp.1183-1189
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• 2015

본 연구는 풍력발전기용 동력 전달부품의 테이퍼 연결장치 설계에 관한 것이다. 요구되는 전달토크의 크기, 동력을 전달하는 축의 직경, 테이퍼 링의 접촉면적 그리고 압축 면압을 부과하기 위한 볼트의 체결력 등이 테이퍼 연결장치 설계의 주요 변수이다. 테이퍼 연결의 계산은 축대칭 평면 변형률 조건의 복합링 구조로 가정하여 응력과 변형량을 계산 하였다. 축에 작용하는 면압은 요구 전달동력을 이용하여 계산하였고, 보강링에 작용하는 면압은 축과 허브의 변형량 일치 조건을 이용하여 계산하는 방법을 제안하였다. 복합링의 수식으로 구한 반경방향의 변형량으로 테이퍼 각도를 고려한 축방향 습동거리를 계산하였다. 수식으로 구한 응력과 상대습동거리의 타당성을 검증하기 위하여 유한요소해석을 수행하였으며 축방향의 하중이 발생하는 테이퍼면에서 원주방향의 응력이 최대 10% 수준의 오차를 보이고 있으나 그 외 응력분포와 상대습동거리는 수식적인 방법과 해석적인 방법이 일치함을 확인하였다.

• International Journal of Oral Biology
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• 제43권1호
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• pp.5-11
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• 2018

Recent findings indicate that Type 2 taste receptors (T2Rs) are expressed outside the gustatory system, including in the gastrointestinal tracts and the exocrine glands, such as the submandibular (SM), parotid (P), lacrimal (L) glands and pancreas (PC). Specifically, T2Rs are found in some of the gastrointestinal endocrine cells, and these cells secreted peptide hormones in response to stimulation by bitter-tasting compounds. The results show that T2Rs may have significant physiological roles besides bitter taste reception. The functions of the T2Rs in the exocrine glands remain poorly understood. An expression levels analysis of T2Rs will help to determine those functions in the exocrine glands. The expression levels of the T2Rs in the exocrine glands were discovered via the qPCR. C57BL/6J mice of 42~60-day-old were used. Messenger RNAs were extracted from S, P, L and PC. Cloned DNAs were synthesized by reverse transcription. Quantitative PCRs were performed using the SYBR Green method. The expression levels of the T2Rs were calculated as relative expression levels to that of the GAPDH. The statistical significance among the observed exocrine glands was tested using the variance analysis (ANOVA test). Tas2r108, out of murine 35 T2Rs, was the most highly expressed in every observed exocrine gland. This finding was similar to previous results from tongue papillae, but the expression levels were lower than those of the tongue papillae. Tas2r137 of SM, P, L and PC were expressed a little lower than that of tongue papillae. The T2Rs in the exocrine glands may play slightly different roles from those in the tongue. We suggest that physiological studies such as a patch clamp and functional $Ca^{2+}$ imaging of acinar cells are necessary for understanding the Tas2r108 functions.

• Archives of Pharmacal Research
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• 제29권11호
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• pp.998-1005
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• 2006

The effects of imipramine on A-type delayed rectifier $K^{+}$ currents and ATP-sensitive $K^{+}\;(K_{ATP)$ currents were studied in isolated murine proximal colonic myocytes using the whole-cell patch-clamp technique. Depolarizing test pulses between-80 mV and +30 mV with 10 mV increments from the holding potential of-80 mV activated voltage-dependent outward $K^{+}$ currents that peaked within 50 ms followed by slow decreasing sustained currents. Early peak currents were inhibited by the application of 4-aminopyridine, whereas sustained currents were inhibited by the application of TEA. The peak amplitude of A-type delayed rectifier $K^{+}$ currents was reduced by external application of imipramine. The half-inactivation potential and the half-recovery time of A-type delayed rectifier $K^{+}$ currents were not changed by imipramine. With 0.1 mM ATP and 140 mM $K^{+}$ in the pipette and 90 mM $K^{+}$ in the bath solution and a holding potential of -80 mV, pinacidil activated inward currents; this effect was blocked by glibenclamide. Imipramine also inhibited $K_{ATP}$ currents. The inhibitory effects of imipramine in A-type delayed rectifier $K^{+}$ currents and $K_{ATP}$ currents were not changed by guanosine 5-O-(2-thiodiphosphate) ($GDP{\beta}S$) and chelerythrine, a protein kinase C inhibitor. These results suggest that imipramine inhibits A-type delayed rectifier $K^{+}$ currents and $K_{ATP}$ currents in a manner independent of G-protein and protein kinase C.

• 한국균학회지
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• 제26권1호통권84호
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• pp.103-107
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• 1998

Coprinus cinereus에서 tryptophan 생합성에 관여하는 trp2 유전자의 이종 버섯에서의 발현여부를 조사하였다. 유전자 상동성을 확인하기 위하여, C. cinereus trp2 유전자를 함유하는 재조합 DNA인 pHIONA8을 probe로 사용하여 southern blot을 한 결과 S. commune 1-71의 tryptophan 생합성 유전자와 상당한 homology가 있는 것으로 나타났다. pHIONA8 DNA를 이용한 S. commune T1(tryptophan 영양요구주)의 상보성 검증에서는 pertri-dish당 약 50개의 형질전환체가 나타났으며, 이들 형질전환체는 다른 S. commune의 다른 화합성 균주와의 교배 실험에서 clamp cell의 유도와 동시에 약 $2{\sim}3$주만에 자실체를 관찰하였다.

• 조명전기설비학회논문지
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• 제13권1호
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• pp.46-52
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• 1999

본 논문은 NPC 인버터의 구조를 위한 유도전동기 구동 시스템의 새로운 히스테리시스 전휴 제어기법을 제안하였다. 이러한 NPC 구조를 가지는 히스테리시스 전류제어 인버터의 구조는 각상의 인버터 출력전압을 제어하는 주 스위칭 소자와 주 스위치 OFF시에 출력전압을 0전위로 유지시키기 위한 보조 스위칭 소자로 구성된다. 이와 같이 제안된 HNPC (Hysteresis current controlled Neutral Point Clamped) 스위칭 기법은 1차, 2차 전류밴드를 가지며, 전압스위칭 신호는 1차 밴드와 실제전류의 비교 결과로서 발생하고, 2차 밴드와 실제전류의 비교 결과는 전압 스위칭 신호의 상위와 하위를 스위치의 동작을 결정하는 신호로서 동작한다. 이러한 스위칭의 결과로서 제안된 인버터 시스템의 출력 파형은 기존의 전류제어 방식과 비교하여 적은 고조파 성분의 함유특성을 가지고, 동일한 전류밴드 범위에서 낮은 스위칭 주파수를 가진다. 본 연구에서는 제안된 기법을 유도 전동기 구동 시스템에 적용하므로 이들 특성을 시뮬레이션을 통하여 고찰하였다.

• Journal of Power Electronics
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• 제5권3호
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• pp.233-239
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• 2005

In this paper, a new conceptual circuit configuration of a 3-phase voltage source, soft switching AC-DC-AC converter using an IGBT module, which has one ARCPL circuit and one ARDCL circuit, is presented. In actuality, the ARCPL circuit is applied in the 3-phase voltage source rectifier side, and the ARDCL circuit is in the inverter side. And more, each power semiconductor device has a novel clamp snubber circuit, which can save the power semiconductor device from voltage and current across each power device. The proposed soft switching circuits have only two active power semiconductor devices. These ARCPL and ARDCL circuits consist of fewer parts than the conventional soft switching circuit. Furthermore, the proposed 3-phase voltage source soft switching AC-DC-AC power conversion system needs no additional sensor for complete soft switching as compared with the conventional 3-phase voltage source AC-DC-AC power conversion system. In addition to this, these soft switching circuits operate only once in one sampling term. Therefore, the power conversion efficiency of the proposed AC-DC-AC converter system will get higher than a conventional soft switching converter system because of the reduced ARCPL and ARDCL circuit losses. The operation timing and terms for ARDCL and ARCPL circuits are calculated and controlled by the smoothing DC capacitor voltage and the output AC current. Using this control, the loss of the soft switching circuits are reduced owing to reduced resonant inductor current in ARCPL and ARDCL circuits as compared with the conventional controlled soft switching power conversion system. The operating performances of proposed soft switching AC-DC-AC converter treated here are evaluated on the basis of experimental results in a 50kVA setup in this paper. As a result of experiment on the 50kVA system, it was confirmed that the proposed circuit could reduce conduction noise below 10 MHz and improve the conversion efficiency from 88. 5% to 90.5%, when compared with the hard switching circuit.

• 전력전자학회논문지
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• 제10권4호
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• pp.380-387
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• 2005

본 논문에서는 능동 클램프형 포워드 DC-DC 컨버터를 이용하여 저전압 대전류에 적합한 고전력밀도의 개방형 전원 장치를 구성하고 그 특성에 대해 보고한 것이다. 전원장치의 입력전압은 통신기용에 적합한 36-75V이며, 출력은 3.3V, 100W급으로 하였다. 대전류에서 전도손실을 저감시키기 위해서 동기정류 방식을 사용하였으며 시스템의 과전류 보호기능을 향상시키기 위해서 고정밀 PCB 저항을 이용한 정전류제어기가 이용되었다. 시험용 전원장치는 통신기용 온보드 전원장치에 적합하도록 높이를 8m 이하, 크기는 quarter brick (58x37mm) 사이즈로 제작되었으며 그 결과 $95W/in^3$ 이상의 전력밀도와 90.6$\%$의 효율, 0.07$\%$ 이하의 전압안정도를 구현하였다.