The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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The Studies on the Heterogenous Gene Expression in Schizophyllum commune

치마버섯에서 이형 유전자 발현에 관한 연구

  • Park, Dong-Chul (Department of Food Science and Technology, Kimchun Junior College) ;
  • Kim, Hyun-Jeong (Department of Food and Nutrition, Yeungnam University) ;
  • Kim, Ok-Mi (Department of Food and Nutrition, Yeungnam University) ;
  • Bae, Jun-Tae (Department of Food and Nutrition, Yeungnam University) ;
  • Park, Sun-Hee (Department of Food and Nutrition, Yeungnam University) ;
  • Lee, Byeung-Hun (Department of Food and Nutrition, Yeungnam University) ;
  • Lee, Kap-Rang (Department of Food and Nutrition, Yeungnam University)
  • 박동철 (김천전문대학 식품가공과) ;
  • 김현정 (영남대학교 식품영양학과) ;
  • 김옥미 (영남대학교 식품영양학과) ;
  • 배준태 (영남대학교 식품영양학과) ;
  • 박선희 (영남대학교 식품영양학과) ;
  • 이병훈 (영남대학교 식품영양학과) ;
  • 이갑랑 (영남대학교 식품영양학과)
  • Published : 1998.03.30
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Abstract

The trp2 gene encoding trifunctional enzyme in Coprinus cinereus was investigated the expression in the heterothallic mushroom species. To identify the homology of trp2 gene to Schizophyllum commune and Pleurotus ostreatus, southern hybridization was performed with plasmid pHIONA8 containing C. cinereus trp2 gene as a probe, which resulted in a strong signal indicating an homologous sequence to the chromosomal DNA of S. commune. About 50 transformants per dish was appeared in the complementation test by pHIONA8 using S. commune tryptophan auxotroph as host. In the mating test between transformants and other mating type alleles, the fruiting body of S. commune was formed at $30^{\circ}C$ in $2{\sim}3$ weeks.

Coprinus cinereus에서 tryptophan 생합성에 관여하는 trp2 유전자의 이종 버섯에서의 발현여부를 조사하였다. 유전자 상동성을 확인하기 위하여, C. cinereus trp2 유전자를 함유하는 재조합 DNA인 pHIONA8을 probe로 사용하여 southern blot을 한 결과 S. commune 1-71의 tryptophan 생합성 유전자와 상당한 homology가 있는 것으로 나타났다. pHIONA8 DNA를 이용한 S. commune T1(tryptophan 영양요구주)의 상보성 검증에서는 pertri-dish당 약 50개의 형질전환체가 나타났으며, 이들 형질전환체는 다른 S. commune의 다른 화합성 균주와의 교배 실험에서 clamp cell의 유도와 동시에 약 $2{\sim}3$주만에 자실체를 관찰하였다.

Keywords

References

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