• 한국유기농업학회지
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• 제30권1호
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• pp.89-101
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• 2022

'암기조생'과 '궁천조생'을 수확 후 단기간 상온저장 시 항균 패치의 부패과 발생 억제효과를 검토하고자 수행하였다. 10kg의 종이상자에 과실을 넣은 후 hexanal, cavarcrol, cinnamaldehyde를 단독 또는 혼합한 200 mg/kg과 500 mg/kg의 항균패치를 부착하여 상온저장하면서 '암기조생'은 15일, '궁천조생'은 30일 동안 부패과 발생을 조사하였다. '암기조생'은 200 mg/kg 처리 시 cavarcrol에서 부패과 발생이 1.0%로 낮았다. '궁천조생'은 500 mg/kg에서 cinnamaldehyde와 hexanal+cinnamaldehyde, 200 mg/kg에서는 hexanal+cinnamaldehyde 혼합이 무처리보다 부패과 발생이 2배 이상 적었다. 항균물질은 단독보다는 혼합 패치가 상온저장 시 감귤 부패과 발생을 줄이는 효과가 높았다.

• 한국식품영양과학회지
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• 제39권8호
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• pp.1236-1241
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• 2010

본 연구에서는 기능성이 향상된 감귤 과실주를 개발하기 위해 감귤 가수분해물의 알코올발효 특성을 조사하였다. 전과(A)와 과피를 제거한 과육(B)의 감귤 가수분해물의 알코올 함량은 B 10.83%로 A 10.13%보다 조금 더 높았으며, 당함량은 A $12.98^{\circ}Brix$로 B $11.38^{\circ}Brix$보다 조금 높게 나타났으나 알코올발효 특성에는 큰 차이가 없었다. Hesperidin 함량은 전과와 과피 제거에 따른 큰 차이가 없었으나, narirutin 함량은 A가 $281.31\;{\mu}M$로 B $199.05\;{\mu}M$에 비해 매우 높게 나타나, 전과와 과피 제거 조건에서 전과로 설정하였다. 효모 종류에 따른 알코올발효 특성을 조사한 결과, S. bayanus EC-1118에서 알코올 함량 12.75%로 가장 높게 나타났다. 초기산도 0.2%일 때 알코올 함량은 12.40%로 가장 높았으며, 초기산도 0.2% 이상에서는 알코올 함량은 조금 감소하는 경향을 나타내었다. 초기당도 $22^{\circ}Brix$에서 알코올 함량은 11.65%, $24^{\circ}Brix$에서는 12.85%로 가장 높게 나타나 초기당도를 $24^{\circ}Brix$로 설정하였다.

• 한국식품위생안전성학회지
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• 제10권3호
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• pp.189-197
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• 1995

Analytical method for synephrine and octopamine in citrus fruits, drinks containing citrus fruit, and human urine was developed using gas chromatography / mass spectrometry(GC/MS), Silylation with MSTFA, acetylation with MBTFA, and trimethylsilylation with MSTFA followed by trifiuoroacetylation with MBTFA were compared. The selective derivatization of synephrine and octopamine was optimized with two derivatizing reagents ; MSTFA and MBTFA. The ion at m/z 267 was monitored to characterize the benzyl group of the both compounds. Synephrine was detected in the concentrations of 0.46∼1.88 ug/g for citrus fruits and 1.2∼8.1 ug/ml for drinks. The urinary excretion data of synephrine showed the highest concentration at the period of 8-20 hours after drinking orange juices and total amounts of its urinary excretion calculated as a parent compound was 11-14% of a dose during 48 hours. Octopamine was not detected in citrus fruits, drinks, and human urine.

• Natural Product Sciences
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• 제6권4호
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• pp.179-182
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• 2000

Nine compounds were isolated from the fruit Citrus junos. Their structures were elucidated as 9-hydroxy-4-methoxypsoralen, auraptene, limonin, deacetylnomilin, cirsimaritin, narirutin, naringin, hesperidin and neohesperidin by physico-chemical evidences. 9-Hydroxy-4-methoxypsoralen and auraptene have not been reported from C. junos yet.

• 한국식품과학회지
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• 제30권2호
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• pp.306-312
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• 1998

대표적인 제주산 감귤류인 하귤, 당유자, 병귤, 스타치, 지각, 궁천조생, 6품종을 수확시기에 따라 유리당, 유기산, 헤스페리딘, 나린진, 무기물을 각각 분석하였다. 수확시기별 유리당의 함량에는 조금씩 차이를 보였으나 모든 품종에 있어서 과즙내 유리당 중에서 sucrose가 $44.9{\sim}66.0%$로 가장 많이 함유되어 있었고 glucose와 fructose는 $15.7{\sim}25.7%,\;17.5{\sim}30.1%$로 서로 비슷하게 함유되어 있었다. 유리당의 함량은 성숙함에 따라 계속적으로 증가하는 경향을 보였다. 유기산 함량은 감귤이 성숙됨에 따라 점차적으로 감소되며, 이는 산 함량의 변화와 비슷한 경향을 보였다. 감귤과즙에서 검출된 주요한 유기산은 citric acid, malic acid, oxalic acid였으며 전체 유기산 중에 citric acid가 90%이상으로 대부분을 차지하고 있었고, 그 외로 malic acid가 $0.98{\sim}9.45%$ 내외로 함유되어 있었고 oxalic acid가 3.58% 이하로 미량 함유되어 있었다. 품종별 과즙과 과피내의 flavonoid계 성분인 naringin과 hesperidin의 함량을 분석한 결과 이 두 성분은 과피 중에서 월등히 많이 함유되어 있었고 미숙과일 때 그 함량이 높았으나 착색이 진행됨에 따라 점차적으로 감소하는 경향을 보였다. 감귤을 이용한 가공품 제조시에는 완숙과를 사용해야 가공품의 혼탁과 쓴맛이 적을 것으로 예상되었다.

• 생물환경조절학회지
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• 제31권4호
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• pp.261-269
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• 2022

본 연구는 '암기조생' 감귤 과실의 표면온도와 일사량 간의 상관관계를 분석하여 일소가 유발되는 과실 표면온도를 예측하고 일소 발생을 경감시키고자 수행하였다. 이를 위해 기온이 31℃일 때 30분 간격으로 5분 미세살수와 1% 탄산칼슘, 4% 카올린 용액을 10일 간격으로 3회 엽면살포하여 일소과 발생과 과실 품질을 조사하였다. 과실 표면온도는 일사량과 R = 0.788(p < 0.01)로 유의한 정의 상관관계를 나타냈으며 한낮에는 기온보다 최고 16℃가 높았다. 과실 표면온도를 추정하는 회귀식 y = 0.099 × (대기온도) + 0.018 × (일사량) + 20.779(R = 0.687, p < 0.01)을도출하였다. 30분 간격으로 5분 미세살수시수관 내부 온도가 무처리보다 5.1℃ 낮았다. 일소과 발생률은 미세살수구가 2.1%로 무처리구보다 2배 이상 낮았고 통계적으로 유의성을 나타냈으나, 과실 품질은 처리간에 비슷하였다. 햇빛을 반사하거나 미세살수를 실시하여 과실 표면온도를 낮추면 일소 발생을 줄일 수 있을 것으로 보인다.

• Molecules and Cells
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• 제26권3호
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• pp.319-322
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• 2008

Little is known about the chromosomal variability and polymorphism existing in mitotic chromosomes of Citrus, mainly due to lack of reliable chromosomal markers and small chromosome size. To test the hypothesis of chromosomal polymorphism and provide the foundation of the genome organization in the Citrus cultivars, we have developed molecular cytogenetic markers for 13 Citrus species collected from Jeju island, Korea. In this study, we demonstrated that the chromosomal locations of cytogenetic markers are quite variable and extremely polymorphic, in contrast to the previous studies. The data obtained in this study will be of utmost importance in cytological systematics and karyotyping of the Citrus species.

• The Plant Pathology Journal
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• 제40권2호
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• pp.115-124
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• 2024

Citrus cultivation plays a pivotal role, making a significant contribution to global fruit production and dietary consumption. Accurate identification of viral pathogens is imperative for the effective management of plant viral disease in citrus crops. High-throughput sequencing serves as an alternative approach, enabling comprehensive pathogen identification on a large scale without requiring pre-existing information. In this study, we employed HTS to investigate viral pathogens infecting citrus in three different regions of South Korea: Jejudo (Jeju), Wando-gun (Wando), and Dangjin-si (Dangjin). The results unveiled diverse viruses and viroids that exhibited regional variations. Notably, alongside the identification of well-known citrus viruses such as satsuma dwarf virus, citrus tatter leaf virus, and citrus leaf blotch virus (CLBV), this study also uncovered several viruses and viroids previously unreported in Korean citrus. Phylogenetic analysis revealed that majority of identified viruses exhibited the closest affilations with isolates from China or Japan. However, CLBV and citrus viroid-I-LSS displayed diverse phylogenetic positions, reflecting their regional origins. This study advances our understanding of citrus virome diversity and regional dynamics through HTS, emphasizing its potential in unraveling intricate viral pathogens in agriculture. Consequently, it significantly contributes to disease management strategies, ensuring the resilience of the citrus industry.

• The Plant Pathology Journal
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• 제17권1호
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• pp.62-66
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• 2001

Detection of Xanthomonas axonopodis pv. citri (Xac) on citrus fruits for exporting is usually made by bacteriophage test (BPT) to demonstrate the pathogen-free status. BPT has rather time-consuming and complicate procedures for dealing with massive samples to be inspected. In this study, enzyme-linked immunosorbent assay (ELISA) was applied to detect Xac on fruits, and compared with BPT. In ELISA, positive reactions occurred in the bacterial densities of $3\times10^5$ cells/ml or more. To detect the bacterial infection on citrus fruits with a density of lower than $3\times10^5$ cells/ml, the bacterial suspensions were mixed with fruit rinse water and incubated in broth medium. Ordinary peptone sucrose broth (PSB) was not a proper medium for increasing Xac density specifically enough to be detect by ELISA. On the other hand, modified PSB (MPSP) amended with Fe-EDTA (0.25 g/$\ell$) and 2.5% potato-dextrose broth sufficed to differentiate uninfected and infected citrus fruits by ELISA after 24 h incubation of the fruit rinse water. Using various citrus samples from infected and uninfected fields, efficiencies in detecting Xac on fruits were compared between ELISA and BPT. For infected fruits samples, ELISA detected Xac by 100%, while BPT by about 44%, indicating that the detection efficiency was improved by 23.5% by ELISA, compared to BPT. In addition, ELISA has simpler procedures for testing and is less time-consuming than BPT, suggesting that ELISA may be accurate and simple method to detect Xac on citrus fruits.

• Journal of Applied Biological Chemistry
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• 제44권3호
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• pp.143-146
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• 2001

Eight flavonoids, including rutin, naringin, hesperidin, quercetin, hesperetin, nobiletin, 3,5,6,7,8,3',4'-methoxylated flavone, and tangeretin, in the peels of satsuma mandarin (Citrus unshiu) species of Halla, Gungcheon, Hungjin, Namgam-20, Illnam-1, and Chungdo harvested between August and December were analyzed through HPLC. Hesperidin content of Halla harvested during early maturation was 28.70 mg/g, and was the highest among the tested citrus fruits. Rutin content of Hungjin harvested during early maturation was 2.66 mg/g. Naringin in all citrus species and hesperetin in Halla, Gungchun, Namgam-20, and Chungdo were only detected in the peel of fruits harvested during early maturation. Hesperidin and rutin were detected mainly in all citrus species, and other flavonoids in trace. Flavonoid content in the peel of fruits was high during early maturation. Flavonoid contents in the peels of all fruit samples were generally high in the early stage of maturation, which then decreased rapidly.