• Journal of Aquaculture
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• v.3 no.1
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• pp.31-37
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• 1990

Ten strains of tilapiine species from genus Oreochromis were cytogenetically studied for genetic stock identification. Both the chromosome numbers(2n=44) were identical in all 10 strains. Heteromorphic sex chromosome pair were not found in any strains. Nuclear volumes vary between O. niloticus(21.0 $\mu$ $m^3$) and O. aureus(22.4 $\mu$ $m^3$)

• Journal of the Korean Society of Tobacco Science
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• v.7 no.1
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• pp.93-96
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• 1985

This study was conducted to establish the maternal haploid method for the practical breeding of M tabacum using the interspeciflc hybridization between M tabacum and N. aflicana. The frequency of surviving seedling per seed capsule of interspecific hybridization was 4.15. Among them, the frequencies of maternal haploid and hybrid were 1.20 and 2.95, respectively. The chromosome numbers of n=24 for maternal haploid and 2n=47 for hybrid were identified in surviving seedling from interspecific hybridiztion. Except the chromosome number, distinguishable morphological differences of material haploid from hybrid were observed at seedling stage.

• Parasites, Hosts and Diseases
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• v.37 no.4
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• pp.237-241
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• 1999

In order to analyze chromosome numbers and karyotypes of intestinal trematodes belonging to the genus, Metagonimus, the gonad tissues of M. takahashii, M. miyatai, and M. yokogawai were prepared and examined. The number of bivalents in the first meiotic division of M. takahashii was nine (n＝9). The diploid number of M. miyatai was observed to be eighteen (2n＝18) and their chromosomes consisted of one pair of metacentric, 7 pairs of submetacentric, and one pair of telocentric chromosomes. The diploid number of M. yokogawai was thirty-two (2n＝32) and the chromosome complements were composed of two pairs of metacentric, 11 pairs of submetacentric, and three pairs of subtelocentric chromosomes. These results could be a supporting evidence for the validity of the new species, M. miyatai, distinct from M. yokogawai.

• Journal of Plant Biology
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• v.15 no.1
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• pp.28-32
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• 1972

Young haploid leaf derived from the anthers of tobacco plant was cultuerd and plantlets of various ploidies were obtained. When the leaf was put on the medium supplemented with kinetin as growth regulator, plantlets developed directly from the leaf, and the plants coming out in early stage of culture were all haploid. Plants developing in later stage were mostly haploids with some exception of diploid and aneuploid. Leaves were also cultured on the callus-inducing media supplemented with 2,4-D and kinetiion, and the calluses were sub-cultured for six months. Plants developed from these calluses were mostly aneuploids of various chromosome numbers. In view of the fact that the plants directly developed from the leaf were all haploid, the tissue of the original leaf explant was assumed to be uniform as far as chromosome number was concerned. On the other hand, it seemed that the occurrence of various ploidies in the plants derived from the calluses of same origin was the result of the influence of in vitro culture. Apical meristem tissues and various multicellular bodies were formed in the epidermal and inner mesophyll tissues as well as in the sub-epidermal cells.

• Journal of Plant Biology
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• v.12 no.1
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• pp.35-48
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• 1969

This study was made on the taxa Chrysanthemum zawadskii complex that grow in South Korea on the basis of chromosomes, epidermis, pollens and gross morphology. I have found four types of chromosome numbers, 36, 45, 54, and 72 as a polyploidal series. Even though the gross morphology was quite similar almost the same gross morphology, chromosome number was different among the taxa. The taxa of 36 chromosomes present broad and fine lobed leaves which grow separately, broad leafed taxon in the mainland of Korea and the other's in Ullungdo Island which is isolated form the mainland in the East Sea. The taxa of 54 chromosomes are also present in the broad and in the fine lobed leaves. The fine lobed leave taxon grows in central to northern Korea and in the high altitude of mountains. Broad leafed taxon grows in central to southern Korea and comparatively lower altitude of the mountains. The taxon of 72 chromosomes is grown in the high altitude of Mt. Hallasan which is isolated from the mainland of Korea. According to this study of Chrysanthemum zawadskii complex, I have arranged the scientific names, as Chrysanthemum zawadskii subsp. latilobum, subsp. acutilobum, subsp. naktongenese, subsp. lucidum, subsp. coreanum and hybrid between subsp. acutilobum X subsp. latilobum.

• Korean Journal of Poultry Science
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• v.13 no.2
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• pp.167-172
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• 1986

Chromosome size, number and shape were studied by the centromeric index, the arm ratio and the relative length of chromosome. The chromosomes of 50 early chick embryos which were derived from a pure line of Single Comb White Leghorns were examined. Using a colchicine, hypotonic treatment, fixation and air-drying technique, the clear prometaphase figures were obtained from the whole embryo. The results of the present investigation of chromosome pairs were as follows, 1. Pair 1 and 2; metacentric and submetacentric chromosomes which could be clearly distinguished from each other by size. 2. Pair 3 and 4: acrocentric chromosomes of similar length but the 4th pair had a distinct short arm which was not present in the 3rd. 3, Pair 5; metacentric sex chromosomes, 2 chromosome had relative 5th length but the W chromosome had slightly shorter length than 7th pair of chromosomes. 4. Pair 6; acrocentric chromosomes similar in shape to pair 3 but of little more than half the size. 5, Pair 7 and 8; acrocentric chrocentric but the 7th pairs had a definite short arm. 6. Pair 9; similar length to the 7, 8 pairs but had a medially placed centromere. 7. microchromosomes of 30 pairs ; nearly all acrocentric chromosomes which appeared as paired dots. The total number of diploid was appeared to 72-78. But a number of observations presented the total diploid number in 78 (58%). The inconstancy in number observed in this study was presumably due to the minute size of the microchromosomes. Thus, the modal numbers for the diploid chromosome was at least 78.

• Korean Journal of Plant Taxonomy
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• v.33 no.4
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• pp.339-357
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• 2003

The general morphological characters and chromosome numbers about 7 taxa of Korean Allium sect. Sacculiferum were reviewed. From the results, authors elucidated the key character as well as the range of variations in each taxon, and considered the relationships among taxa in this section. Clear taxonomic treatments also carried out based on the type specimens and the original descriptions. Shape and growing pattern of leaves, size of inflorescences, shape of perianths and tepals, as well as chromosome numbers were key characters in identifying the taxa and in estimating their relationships. On the basis of these characters, A. thunbergii var. thunbergii, A. sacculiferum and A. deltoide-fistulosum were recognised as a distinct species respectively. Hal-la-bu-chu which had been misidentified as A. cyaneum (sect. Reticulato-bulbosa) in Korea was proved to be A. taquetii and belonged to the sect. Sacculi/erum. In addition, A. cyaneum var. deltoides, had been described as a variety of A. cyaneum, was recombined as a variety of A. thunbergii [A. thunbergii var. deltoides (S. Yu, W. Lee et S. Lee) H. J. Choi et B. U. Oh] with the new Korean name of 'Se-mo-san-bu-chu'. In conclusion, Korean sect. Sacculiferum was composed of five species and two varieties. A key to identify the taxa in this section was provided.

• The Korea Journal of Herbology
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• v.22 no.4
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• pp.287-300
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• 2007

Objectives : The genotoxicity of extract of "Hyeonggaeyeongyotang", a polyherbal formula has been used as a tonic agents in oriental medicine was tested. Methods : Extract of "Hyeonggaeyeongyotang" was tested by In Vitro Chromosome Aberration Test, Bacterial Reverse Mutation Assay and Micronucleus test according to OECD Guidelines and KFDA Guidelines [2005-60]. Results : The obtained results were as follows: 1. Chromosome Aberration Test: No significant changes in the number of aberrant metaphases having structural and number of aberrations were detected in all concentrations of "Hyeonggaeyeongyotang" extracts treated in this study. 2. Bacterial Reverse Mutation Assay: No significant increases in the number of revertant colonies compared to its negative control were detected in all concentrations of "Hyeonggaeyeongyotang" extracts treated in this study against all 5 strains except for $50{\mu}g/ml$ treated group where significantly decreases in colony numbers were detected agains all five strains used in this study as pharmacological effects not genotoxicity. 3. Micronucleus test: No significant changes in the number of micronucleated polychromatic erythrocytes among 2000 polychromatic erythrocytes compared to negative control were detected in all "Hyeonggaeyeongyotang" extracts-dosing groups tested. Conclusions : From above-mentioned results, it is concluded that "Hyeonggaeyeongyotang" extracts have not any genotoxicity against In Vitro Chromosome Aberration Test, Bacterial Reverse Mutation Assay and Micronucleus test.

• Korean Journal of Medicinal Crop Science
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• v.11 no.4
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• pp.311-315
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• 2003

This study was carried out to compare chromosomal characteristics between Atractylodes japonica and A macrocephala. Cytogenetic analysis was conducted based on karyotype analysis and physical mapping using fluorescence in situ hybridization. As a result of karyotype analysis by feulgen staining, somatic chromosome numbers of A. japonica and A. macrocephala were 2n=24. The length. of the mitotic metaphase chromosomes of A. japonica ranged from $0.70\;to\;1.60{\mu}m$ with a total length. of $12.11{\mu}m$ and the homologous chromosome complement comprised six metacentrics, five submetacentrics and one subtelocentrics. On the other hand, the length of the mitotic metaphase chromosomes of A. macrocephala ranged from $0.90\;to\;2.35{\mu}m$ with a total length of $16.58{\mu}m$ and the homologous chromosome complement comprised seven metacentrics and five submetacentrics. The total length of A. japonica chromosomes was shorter than that of A. macrocephala, but A. japonica had one subtelocentrics (chromosomes 4) different from A. macrocepha1a. chromosomes. The F1SH technique using 17S and 5S rDNA was applied to metaphase chromosomes. The signals for 17S rDNA were detected on the telomeric regions of chromosomes 4 and 5 in both A japonica and A. macrocephala. The 5S rDNA signal was found in the short arm of chromosome 1.

• Journal of Species Research
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• v.2 no.1
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• pp.91-98
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• 2013

An investigation of the karyotypes of two species of the genus Brachymystax (B. lenok and B. tumensis) has been done for the Russia Primorye rivers running to the East Sea basin, and others belonging to Amur basin. Based on the analysis of two species chromosome characteristics, combined with original and literary data, four cytotypes have been described. One of these cytotypes (Cytotype I: 2n=90, NF=110-118) was the most common. This common cytotype belongs to B. tumensis from the rivers of the East Sea basin and B. lenok from the rivers of the Amur basin, i.e. extends to the zones of allopatry. In the rivers of the Amur river basin, in the zone of the sympatric habitat of two species, each taxon has karyotypes with different chromosome numbers, B. tumensis (2n=92) and B. lenok (2n=90). Because of the ability to determine a number of the chromosome arms for these two species, additional cytotype have been identified for B. tumensis: Cytotype II with 2n=92, NF=110-124 in the rivers basins of the Yellow sea and Amur river and for B. lenok three cytotypes: Cytotype I: 2n=90, NF=110 in the Amur river basin; Cytotype III with 2n=90, NF=106-126 in the Amur river basin and Cytotypes IV with 2n=92, NF=102 in the Baikal lake.