• 환경생물
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• 제24권1호
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• pp.81-88
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• 2006

열대 남태평양에 위치한 축 라군에서 flow cytometry와 HPLC를 이용 초미소 식물플랑크톤 조사를 하였다. Synechococcus, Prochlorococcus 그리고 picoeukaryotes의 풍도와 지시색소 분포 양상을 분석하였고 추정된 탄소 생체량에서의 기여율을 조사하였다. 남세균인 Synechococcus와 Prochlorococcus는 축 라군에서 대비되는 분포 특성을 보였다. Synechococcus는 웨노섬 주변 정점에서 높은 풍도를 보인 반면, 북동수로 쪽으로 멀어지며 풍도가 감소하였다. Prochlorococcus는 이와 반대의 분포특성을 나타냈으며, picoeukaryotes는 특정적인 수역별 변화를 보이지 않았다. Prochlorococcus의 지시색소인 divinyl chlorophyll a (Chl. $\alpha2$)는 $1.2\sim180.3\;ng\;L^{-1}$의 농도 범위를 보였으며, 웨노섬 주변 정점들보다 북동수로에 접한 정점들에서 높게 나타났다. 이는 Prochlorococcus의 풍도와 유사한 분포로서, chl. a2가 생체량의 좋은 지표임을 나타냈다. 반면 zeaxanthin은 $61.4\sim135.8\;ng\;L^{-1}$의 농도 범위로 정점별 변화가 풍도변화에 비해 작은 특징을 보였는데 이는 Prochlorococcus로부터 유래된 zeaxanthin의 영향으로 판단된다. Synechococcus의 추정된 탄소 생체량은 전체 초미소 식물플랑크톤 생체량의 68%를 차지하였다. Prochlorococcus와 picoeukaryotes는 각각 17.1%와 14.9%의 생체량을 나타냈다.

• 한국수산과학회지
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• 제10권2호
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• pp.125-135
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• 1977

Recently, culture of Undaria pinnatifida, one of the representative esculent sea weed, has been prevailing in tile east and south coasta of Korea and reached the mass culture stage. In this study, compositional quality factors for food were studied and the contributory effects of blanching and pigment fixatives in the quality retention of cultured Undaria pinnatifida are discussed. When the place and time of harvesting were the same, cultured pinnatifida showed scarce difference in the chemical composition comparing to tile naturally grown Undaria pinnatifida, but cultured Undaria pinnatifida shelved a considerable difference depending upon the cultured places. In the chemical composition of Undaria pinnatifida, the alginic acid comprising about $40\%$ of the whole solid materials seemed to be responsible for the compositional puality. The chlorophyll and carotenoid content of the clutured Unaria pinnatifida were considerably lower than that of the naturally grown Undaria pinnatifida and wass inferior in puality by color to the naturally grown one. Dried Undaria pinnatifida contained a considerable amount of amino-N, mannitol, and soluble minerals and it is considered that these components play a great role in the relish effects. It could also be evaluated as a good albuminous source for food science the dried pinnatifida contains about $18\%$ of crude protein. In the analysis of free amino acid composition of dried Undaria pinnatifida, the naturally growm samples showed so what higher levels in all amino acid content than the cultured samples. The contents of theronine, alanine, and glutamic acid were major in quantity wherease histidine cysteine, tyrosine, and phenylalanine were minor. The contents of such amino acids like serine and proline were particularly low or undetectable. The results of amino acid analysis of the acid hydrolysates of dried Undaria pinnatifida in quantity of individual amino acid showed te same pattern as that of free amino acid. It is noticed that Undaria pinnatifida seemed to contain good quality protein since the contents of essential amino acids were considerably higher and uniform. By blanching the fresh sample, the water soluble components brought about cousiderable loss, and, particularly, it was noteworthy that both mannitol and soluble minerals apparently decreased. In the pigment analysis of the dried sample, blanching was effective to retain chlorophyll and carotenoid. The addition of pigment fixatives in blanching solution such as Ca-gluconate, Ca-carbonate, and Ca-hydroxide did not exhibit much effect on the pigment retention except that Ca-carbonate shelved some effect only in the early stage of storage.

• 한국환경농학회지
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• 제33권4호
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• pp.344-349
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• 2014

BACKGROUND: Purpose of the study was to establish the extinction coefficients of chlorophyll a and b in N,N-dimethylformamide(DMF) and Dimethylsulfoxide(DMSO) solvents and to find out the conditions of optimal extraction temperature and time in citrus leaves. METHODS AND RESULTS: Chlorophyll a and b standards were dissolved in DMF and DMSO. Extinction coefficients of chlorophyll pigments were determined and their contents were quantified using spectrophotometer. Chlorophyllous pigments of citrus(Citrus unshiu Marc. cv. Okitsu wase) leaves were extracted at 25, 40, 60 and $80^{\circ}C$ for 4, 6, 8, 24 and 48 hours to determine the optimal extraction condition. CONCLUSION: The extinction coefficients of chlorophyll a(Chl a) and chlorophyll b(Chl b) of DMF extracts for high extinction wavelength were 663.8 and 647.2 nm. Similarly, the high extinction wavelength of DMSO extracts were 665.8 and 649.0 nm for chl a and b respectively. Chl a, Chl b and total chlorophyll content of DMF extracts were Chl a = $12.10A_{663.8}-2.74A_{647.2}$, Chl b = $21.94A_{647.2}-5.06A_{663.8}$ and total $Chl=19.193A_{647.2}+7.04A_{663.8}$. Similarly, Chl a, Chl b and total Chl of DMSO extracts were Chl a = $14.53A_{665.8}-5.40A_{649.0}$, Chl b = $26.98A_{649.0}-7.11A_{665.8}$ and total $Chl=21.58A_{649.0}+7.43A_{665.8}$. The chlorophyll extracts of DMF and DMSO were very stable in dark. High chlorophyll contents of citrus leaves were found at $80^{\circ}C$ extraction for 6 hours in DMF and at $80^{\circ}C$ extraction for 24 hours in DMSO. However, the chlorophyll content was decreased significantly after 8 hours in DMF extraction while it was remained up to 30 hours in DMSO extraction.

• 한국환경과학회지
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• 제5권2호
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• pp.153-170
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• 1996

멕시코 만에서 1987~1988년에 부유 및 칩강입자에 존재하는 광합성 색소 및 그 분해산물은 HPLC를 이용하여 측정하였다. 클로로필과 카로틴계열 및 그 분해산물의 단기 침하율이 Cold-Core Ring 과 Warm-Core Ring에서 비교 분석되었다. 클로로필 a는 CCR과 WCR에서 공통적으로 우세한 포르피린이었으며 카로틴계열 색소 중에는 19'-hwxanoyloxyfucoxanthin이 우세하였다. 초식자의 섭이과정에서 생성되는 것으로 알려진 phaeophobide a가 조사지역 침강입자에 우세하게 나타나는 분해색소 이었따. CCR에서의 전체색소 침하율은 WCR보다 한단게 높게 나타났다. WCR에서 색소현존량은 하루에 1% 이하가 유광밖으로 침하 되었는데 이 사실은 멕시코 만에서 부유고형입자의 재순환은 빠르게 일어나고 있지 않음을 의미한다.

• 공업화학
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• 제26권5호
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• pp.569-574
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• 2015

미세조류 Arthrospira platensis (A. platensis)는 색소(chlorophyll, carotenoids), 지질을 다량 함유하여 기능성 식품 생산에 중요한 자원으로 평가받고 있다. A. platensis에 조사선량 240 kGy의 전자빔을 조사하여 균주를 개량하고자 하였으며, 스크리닝을 통해 지질 함량이 높은 EM24를 선별하였다. EM24는 세포생장률 및 색소농도가 야생균주에 비해 약 1.2배 증가함을 확인하였다. 또한 형광강도법으로 측정 후 triolin을 이용해 세포 내 지질 농도를 정량하고자 하였다. EM24의 지질 농도는 62.9 mg/L로 야생균주(38.9 mg/L)에 비해 약 1.5배 증대되었다. 세포 내 지방산 또한 gamma-linoleic acid(GLA) 함량이 약 1.5배 증가하였다.

• 한국생활과학회지
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• 제24권6호
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• pp.859-871
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• 2015

The purpose of this study is to examine the influence of the pigment characteristic and changes in dying conditions on the dying properties by extracting Chelidonium majus var. asiaticum using distilled water and ethanol as solvents. Changes in dying conditions include variations in dye concentrations, dyeing temperatures and time on dye uptake, and K/S Value was compared according to these changes. Additionally, color changes were observed through mordant. Ultraviolet-visible spectrum was used to investigate the pigment characteristic, and as a result, tannin was identified in distilled-water-extract, whereas berberine and chlorophyll were identified in ethanol-extract. In FT-IR analysis, tannin in distilled-water-extract was verified as hydrolyzable tannin. For ethanol extract, chlorophyll was verified through absorption band of C-H, which is aliphatic spectrum around $2920cm^{-1}$ and $2850cm^{-1}$. From GC/MS analysis, oil components as well as terpine compounds were detected in ethanol-extract, and this, in turn, brings expectation regarding functionality. When dying in silk, dye uptake increased as concentration of the extract increased, and the optimum dyeing temperature and time were $40^{\circ}C$ and 60 minutes respectively. Dyed fabrics' colors were all basically Y-series colors, and adjustment in brightness and revelation of khaki color were also available depending on the type of the mordant. Color fastness, except for washing fastness, was good in silk dyed with distilled-water-extract. Thus, it can be concluded that by selecting the right extracting method and by doing proper dyeing and mordant according to the needs, these dyed fabrics can be used as eco-friendly, functional clothing material.

• 한국패류학회지
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• 제29권4호
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• pp.291-301
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• 2013

This study researched and compared the sedimentation environment and photosynthetic pigments at Gomso (GS) -bay, Yoja (YJ) -bay and KangJin (KJ) -bay in May, 2012. It was shown that KJ-bay consist of C (clay) and M (mud), and GS-bay consists of Zs (silty sand) and Sz (sandy silt). Averagely, IL of YJ-bay was 4.98%, KJ-bay 6.10%, and GS-bay 1.45%. As for COD concentration, there were no places that exceeded Japanese sediment contamination standard 20 mg/g-dry. As for AVS concentration, in case of KJ-bay, two places exceeded Japanese sediment contamination standard 0.2 mg/g-dry. If we look into the average C/N ratio of bays, YJ-bay showed 8.50, KJ-bay 6.60, and GS-bay 5.52, thus all of them showed the characteristic of oceanic origin. As for the plankton make-up classified by photosynthetic pigments, diatom was dominant, and both ratios of pigment and C/chlorophyll. a showed the relatively lower distribution at GS-bay than at KJ-bay and YJ-bay. It is judged that this is related to the characteristic of flow-in, and it implies that predation process and decomposition by organic matters actively occurs at YJ-bay and KJ-bay.

• Journal of the korean society of oceanography
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• 제32권1호
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• pp.46-55
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• 1997

The quantitative determination of carotenoids, chlorophylls and their degradation products from marine phytoplankton was performed by reverse-phase high performance liquid chromatography (HPLC). Separated and quantified chlorophylls and their degraded products are chlorophyll a, b, c, chlorophyllide a, phaeophytin a, b and phaeophorbide a. Concentrations of six carotenoids including fucoxanthin, 19'-butanoyloxyfucoxanthin, 19'-hexanoyloxyfucoxan-thin, prasinoxanthin, alloxanthin, zoaxanthin/lutein were also determined from natural field samples by absorbance. Results of pigment analysis of field samples collected from the southern waters of the East Sea on October 8th, 1996 were reported. Concentration of chi a ranged from 7.2 to 180.4 ng/1. Concentration of chi b and chi c ranged from 22.7 to 53.7 ng/1 and from 3.3 to 58.5 ng/1, respectively. Significant concentrations of phaeophytin a, and chlorophyllide a were also detected at different depths. Fucoxanthin, 19'-hexanoyloxyfucoxanthin and zoaxanthin/lutein were the most abundant carotenoids and 19'-butanoyloxyfucoxanthin, prasinoxanthin and alloxanthin were also detected relatively at low concentrations. These results of pigment analyses suggest predominance of diatom, prymnesiophytes and cyanobacteria and presence of crysophyte, green algae (prasinophyte and prochlorophyte), and cryptophyte in the study area on October 8th, 1996, We report prymnesiophyte for the first time as an important biomass component of marine phytoplankton in the study area. Vertical profiles for the concentration changes of the major pigments were also given.

• 한국염색가공학회지
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• 제28권3호
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• pp.195-207
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• 2016

In this study, we examined the influence of the pigment characteristic and dyeing condition on dyeing properties and functionality by using Perilla Frutescens L. Britt extracts, in which ethanol, distilled water and NaOH solution were used as 3 different solvents. Changes in dyeing conditions include variations in dye concentration, dyeing temperature, time and pH on dye uptake, and K/S values were compared according to these changes. Additionally, color changes were observed according to the use and types of mordant. Ultraviolet-visible spectrum was utilized to investigate the pigment characteristic, and as a result, chlorophyll was identified in ethanol extract, whereas tannin was identified both in distilled water extract and NaOH solution extract. By using FT-IR analysis, these tannins in distilled-water-extract and NaOH solution extract were verified to be hydrolyzable tannin. When dyeing silk, dye uptake increased as dye concentration, dyeing temperature and time increased, while it decreased as pH of the extract increased. Fabrics dyed without a mordant produced Y-series colors, and fabrics dyed with mordants showed various colors depending on the mordant types. Even though color fastness to washing and light was unsatisfactory, fastness to rubbing and perspiration showed relatively high grade. Moreover, deodorant ability of dyed fabric improved.

• 한국해양바이오학회지
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• 제6권1호
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• pp.26-30
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• 2014

Chlorella vulgaris, a genus of single-cell green algae, is considered to be a very essential resource for the higher value-added business including functional food and biodiesel, due to its high contents of protein, carbohydrate and lipid. In this study, ultraviolet rays were irradiated in order to induce the mutation of C. vulgaris. After inducing the mutation, UV1-20 mutant, high in lipid was selected and its cell growth rate, dry weight, pigment content and lipid content were measured. The growth rate of the UV1-20 mutant was increased almost 1.5 times than the wild type, but pigment contents of chlorophyll and carotinoid were decreased. In addition, the lipid content of UV1-20 was increased 1.8 times than the wild type. Therefore, C. vulgaris mutant, isolated in this study, is considered to have sufficient potential to be used as a material for the higher value-added business.