Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Isolation of an Arthrospira platensis Mutant Induced by Electron Beam Irradiation and its Characterization

전자빔 조사를 통해 유도된 Arthrospira platensis 변이주 분리 및 특성 분석

  • Choi, Soo-Jeong (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University) ;
  • Lee, Jae-Hwa (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University)
  • 최수정 (신라대학교 의생명과학대학 제약공학과) ;
  • 이재화 (신라대학교 의생명과학대학 제약공학과)
  • Received : 2015.07.07
  • Accepted : 2015.08.20
  • Published : 2015.10.10
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Abstract

Arthrospira platensis has high lipid and pigment (such as chlorophyll and carotenoid) contents and thus evaluated as an important resource in functional food production. The cell growth rate and pigment concentration of EM24 increased by approximately 1.2-fold than those of the wild-type strain (WT). Fluorescence intensity levels in EM24, which were quantified with a lipid triolein standard curve, also increased by approximately 1.5-fold than those in WT (62.9 mg/Lvs. 38.9 mg/L). The analysis of fatty acid profiles indicated that the gamma-linoleic acid level in EM24 increased by 1.5-fold than that in WT.

미세조류 Arthrospira platensis (A. platensis)는 색소(chlorophyll, carotenoids), 지질을 다량 함유하여 기능성 식품 생산에 중요한 자원으로 평가받고 있다. A. platensis에 조사선량 240 kGy의 전자빔을 조사하여 균주를 개량하고자 하였으며, 스크리닝을 통해 지질 함량이 높은 EM24를 선별하였다. EM24는 세포생장률 및 색소농도가 야생균주에 비해 약 1.2배 증가함을 확인하였다. 또한 형광강도법으로 측정 후 triolin을 이용해 세포 내 지질 농도를 정량하고자 하였다. EM24의 지질 농도는 62.9 mg/L로 야생균주(38.9 mg/L)에 비해 약 1.5배 증대되었다. 세포 내 지방산 또한 gamma-linoleic acid(GLA) 함량이 약 1.5배 증가하였다.

Keywords

References

  1. K. Y. Lim, K. Sharma, S. Garg, and P. M Schnk, The race for highly productive microalgae strains, Biofuels, 1(6), 835-837 (2010). https://doi.org/10.4155/bfs.10.65
  2. S.-S. Hong and N.-H. Lee, Growth of Spirulina platensis in effiuents from wastewater treatment plant of pig farm, J. Microbiol. Biotechnol., 3(1), 19-23 (1993).
  3. A. Klanchui, T. Vorapreeda, W. Vongsangnak, C. Khannapho, S. Cheevadhanarak, and A. Meechai, System biology and metabolic engineering of Arthrospira cell factories, Comput. Struct. Biotechnol. J., 4(3), 1-8 (2012).
  4. S. Otles and R. Pire, Fatty Acid Composition of Chlorella and Spiculina Microalgae Species, J. AOAC Int., 84(6), 1708-17148 (2001).
  5. S. cheevadhanarak, N. T. Marsac, J. C. Thomas, M. Tanticharoen, and P. Nomsawai, Light regulation of phycobilicome structure and gene expression in Spirulina platensis C1 (Arthrospira sp. PCC9483), Plant Cell Physiol., 40(12), 1194-1202 (1999). https://doi.org/10.1093/oxfordjournals.pcp.a029507
  6. P. B. Bescos, A. M. Fresno, and J. E. P. Estrada, Antioxidant activity of different fractions of Spirulina platensis protean extract, Farmaco, 56, 497-500 (2001). https://doi.org/10.1016/S0014-827X(01)01084-9
  7. Y.-J. Lee, S.-C. Wok, H.-J. Kim, J.-H. Lee, and M.-R. Kim, Quality Characteristics and Antioxidant Activities of Spirulina Added Korean Rice Cake (Garaeduk) during Storage, Korean J. Food Preserv., 16(1), 8-16 (2009).
  8. J. A. mendiola et al., Screening of functional compounds in supercritical fluid extracts from Spirulina platensis, Food Chem., 102(4), 1357-1367 (2007). https://doi.org/10.1016/j.foodchem.2006.06.068
  9. J.-Y. Kim, H. Joo, and J.-H. Lee, Carbon Dioxide Fixation and Light Source Effects of Spirulina platensis NIES 39 for LED Photobioreactor Design, Appl. Chem. Eng., 22(3), 301-307 (2011).
  10. M. F. Ramadan, M. M. S. Asker, and Z. K. Ibrahim, Functional bioactive compounds and biological activitieds of Spirulina platensis lipids, Czech J. Food Sci., 26(3), 211-222 (2008).
  11. S. R. Ronda and S. S. Lele, Culture conditions stimulating high gamma linolenic acid accumulation by Spirulina platensis, Brazil. J. Microbiol., 39(4), 693-697 (2008). https://doi.org/10.1590/S1517-83822008000400018
  12. H.-J. Park, Y.-H. Kim, and J.-H. Lee, Characterization of Arthrospira platensis Mutants Generated by UV-B Irradiation, Appl. Chem. Eng., 23(5), 496-500 (2012).
  13. Y.-H. Kim, S.-J. Choi, H.-J. Park, and J.-H. Lee, Electron beam-induced mutants of microalgae Arthrospira platensis increased antioxidant activity, Ind. Eng. Chem., 20(4), 1834-1840 (2014). https://doi.org/10.1016/j.jiec.2013.08.039
  14. J.-K. Park and C.-G. Lee, Immobiliztion of Astaxanthin Extracted from Photosynthetic Micro Algae Heamatococcus lacustris, J. Chitin Chitosan., 13(4), 210-214 (2008).
  15. H. Y. Jeong and K.-R. Kim, Strain Improvement Based on Ion Beam-Induced Mutagenesis, Kor. J. Microbiol. Biotechnol., 38(3), 235-243 (2010).
  16. Y. Hiroyasu, S. Akemi, H. Yoshihiro, D. Konosuke, T. Atsushi, and M. Toshikazu, Mutation induction with ion beam irradiation of lateral buds of chrysanthemum and analysis of chimeric structure of induced mutants. Euphytica, 165(1), 97-103 (2009). https://doi.org/10.1007/s10681-008-9767-5
  17. S. Y. Park, H. A. Noh, H. Cho, A. Dumont, S. Ptasinska, A. D. Bass, and L. Sanche, DNA damage by X-ray and low energy electron beam irradiation. J. Kor. Radiat. Prot., 33(2), 53-59 (2008).
  18. S. Ptasinska and L. Sanche, On the mechanism of anion desorption from DNA induced by low energy electrons, J. Chem. Phys., 125(14), 144713-144722 (2006). https://doi.org/10.1063/1.2338320
  19. S. H. Yu, I. H. Cho, S. W. Chang, S. J. Lee, S. Y. Chun, and H. L. Kim, Decomposition Characteristics of Fungicides(Benomyl) using a Design of Experiment(DOE) in an E-beam Process and Acute Toxicity Assessment, J. Kor. Soc. Environ. Eng., 30(9), 955-960 (2008).
  20. J. H. Ryu, H.-S. So, S.-H. Bae, H. S. Kang, B. C. Lee, S.-Y. Kang, H.-Y. Lee, and C.-H. Bae, Genetic Diversity of in vitro Cultured Cymbidium spp. Irradiated with Electron Beam, Kor. J. Breed. Sci., 45(1), 8-18 (2013). https://doi.org/10.9787/KJBS.2013.45.1.008
  21. Y. M. Kim, J.-Y. Kim, S.-M. Lee, J.-M. Ha, T. H. Kwon, and J.-H. Lee, Carbon Dioxide Fixation using Spirulina platensis NIES 39 in Polyethylene Bag, Appl. Chem. Eng., 21(3), 272-277 (2010).
  22. S.-J. Choi, Y.-H. Kim, A. Kim, and J.-H. Lee, Arthrospira platensis mutants containing high lipid content by electron beam irradiation and analysis of its fatty acid composition, Appl. Chem. Eng., 24(6), 628-632 (2013). https://doi.org/10.14478/ace.2013.1085
  23. S.-R. Moon, B.-K. Son, J.-O. Yang, J.-S. Woo, C. M. Yoom, and G.-H. Kim, Effect of Electron-beam Irradiation on Development and Reproduction of Bemisia tabaci, Myzus persicae, Plutella xylostella and Tetranychus urticae, Kor. J. Appl. Entomol., 49(2), 129-137 (2010). https://doi.org/10.5656/KSAE.2010.49.2.129
  24. H.-J. Park, Y.-H. Kim, and J.-H. Lee, Characterization of Arthrospira platensis Mutants Generated by UV-B Irradiation, Appl. Chem. Eng., 23(5), 496 (2012).
  25. W. Chen, M. Sommerfeld, and Q. Hu, Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae, Bioresour. Technol., 102(1), 135-141 (2011). https://doi.org/10.1016/j.biortech.2010.06.076
  26. Y.-H. Kim and J.-H. Lee, Isolation of Arthrospira platensis mutants Producing High Lipid and Phycobiliproteins, Kor. Soc. Biotechnol. Bioeng. J., 27, 172-176 (2012).
  27. E. Bertozzini, L. Galluzzi, A. Penna, and M. magnani, Application of the standard addition method for the absolute quantification of newtural lipids in microalge using Nile red, J. Microbiol. Methods, 87(1), 17-23 (2011). https://doi.org/10.1016/j.mimet.2011.06.018
  28. K. M. Minkova, A. A. Tchernov, M. I. Tchorbadjieva, S. T. Fournadjieva, R. E. Antova, and M. C. H. Busheva, Purification of C-phycocyanin from Spirulin(Arthrospira) fusiformis, J. Biotechnol., 102(1), 55-59 (2003). https://doi.org/10.1016/S0168-1656(03)00004-X
  29. H.-L. Tran, S.-J. Hong, and C.-G. Lee, Evaluation of extraction methods for recovery of fatty acids from Botrycoccus braunii LB 572 and Synechocystis sp. PCC 6803, Biotechnol. Bioprocess Eng., 14(2), 187-192 (2009). https://doi.org/10.1007/s12257-008-0171-8
  30. J.-H. Kim, H.-J. Park, Y.-H. Kim, H. Joo, S.-H. Lee, and J.-H. Lee, UV-induced mutagenesis of Nannochloropsis oculata for the increase of lipid accumulation and its characterization, Appl. Chem. Eng., 24(2), 155-160 (2013).
  31. S. Boussiba, B. Wang, P. P. Yuan, A. Zarka, and F. Chen, Changes in pigments profile in the green alga Haematococcus pluvialis exposed to environmental stresses. Biotechnol. Lett.. 21, 601-604, (1999). https://doi.org/10.1023/A:1005507514694
  32. S. H. Oh, J. G. Han, N. Y. Kim, J. S. Cho, T. B. Yim, S. Y. Lee, and H. Y. Lee, Cell Growth and Lipid Production from Fed-batch Cultivation of Chlorella minutissima according to Culture Conditions, Kor. Soc. Biotechnol. Bioeng. J., 24(4), 377-382 (2009).
  33. D.-K. Kim, J.-A. Shin, and K.-T. Lee, Monitoring of compositions of gamma-linoleic and omega-3 fatty acids in some functional foods consumed in market, CNU J. Agric. Sci., 38(2), 277-284 (2011).