• Journal of Microbiology and Biotechnology
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• v.31 no.9
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• pp.1272-1280
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• 2021

With changes in human dietary patterns, the proportion of high-fat and high-cholesterol foods in the daily diet has increased. As a result, the incidence rate of cholelithiasis is increasing rapidly. Many studies have reported on the crucial role that the intestinal microflora plays in the progression of gallstones. Although the whole herb of Lysimachia christinae, a traditional Chinese medicine, has long been extensively used as a remedy for cholelithiasis in China, its effects on the intestinal microflora remain unknown. Hence, in this study, we investigated the ability of the aqueous extract of L. christinae (LAE) to prevent cholesterol gallstones (CGSs) in model animals by affecting the intestinal microflora. The effects of LAE on body weight, serum lipid profile, visceral organ indexes, and histomorphology were studied in male C57BL/6J mice, which were induced by a lithogenic diet. After the 8-week study, CGSs formation was greatly reduced after LAE treatment. LAE also reduced body weight gain and hyperlipidemia and restored the histomorphological changes. Moreover, the intestinal microflora exhibited significant variation. In the model group fed the lithogenic diet, the abundances of the genera unclassified Porphyromonadaceae, Lactobacillus and Alloprevotella decreased, but in contrast, Akkermansia dramatically increased compared with the control check group, which was fed a normal diet; the administration of LAE reversed these changes. These results imply that L. christinae can be considered an efficient therapy for eliminating CGSs induced by a high-fat and high-cholesterol diet, which may be achieved by influencing the intestinal microflora.

• Journal of Ginseng Research
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• v.44 no.2
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• pp.258-266
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• 2020

Background: Oxidative stress-induced cardiomyocytes apoptosis is a key pathological process in ischemic heart disease. Glutathione reductase (GR) reduces glutathione disulfide to glutathione (GSH) to alleviate oxidative stress. Ginsenoside Rb1 (GRb1) prevents the apoptosis of cardiomyocytes; however, the role of GR in this process is unclear. Therefore, the effects of GRb1 on GR were investigated in this study. Methods: The antiapoptotic effects of GRb1 were evaluated in H9C2 cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, annexin V/propidium iodide staining, and Western blotting. The antioxidative effects were measured by a reactive oxygen species assay, and GSH levels and GR activity were examined in the presence and absence of the GR inhibitor 1,3-bis-(2-chloroethyl)-1-nitrosourea. Molecular docking and molecular dynamics simulations were used to investigate the binding of GRb1 to GR. The direct influence of GRb1 on GR was confirmed by recombinant human GR protein. Results: GRb1 pretreatment caused dose-dependent inhibition of tert-butyl hydroperoxide-induced cell apoptosis, at a level comparable to that of the positive control N-acetyl-L-cysteine. The binding energy between GRb1 and GR was positive (-6.426 kcal/mol), and the binding was stable. GRb1 significantl reduced reactive oxygen species production and increased GSH level and GR activity without altering GR protein expression in H9C2 cells. Moreover, GRb1 enhanced the recombinant human GR protein activity in vitro, with a half-maximal effective concentration of ≈2.317 μM. Conversely, 1,3-bis-(2-chloroethyl)-1-nitrosourea co-treatment significantly abolished the GRb1's apoptotic and antioxidative effects of GRb1 in H9C2 cells. Conclusion: GRb1 is a potential natural GR agonist that protects against oxidative stress-induced apoptosis of H9C2 cells.

• The Journal of Korean Medical History
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• v.28 no.1
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• pp.135-141
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• 2015

The purpose of this study is to analyze vocabulary found in "Donguibogam", one of the medical books in mid-Chosun, through Corpus-based analysis, one of the text analysis methods. According to it, Donguibogam has total 871,000 words in it, and Chinese characters used in it are total 5,130. Among them, 2,430 characters form 99% of the entire text. The most frequently appearing 20 Chinese characters are mainly function words, and with this, we can see that "Donguibogam" is a book equipped with complete forms of sentences just like other books. Examining the chapters of "Donguibogam" by comparison, Remedies and Acupuncture indicated lower frequencies of function words than Internal Medicine, External Medicine, and Miscellaneous Diseases. "Yixuerumen (Introduction to Medicine)" which influenced "Donguibogam" very much has lower frequencies of function words than "Donguibogam" in its most frequently appearing words. This may be because "Yixuerumen" maintains the form of Chileonjeolgu (a quatrain with seven Chinese characters in each line with seven-word lines) and adds footnotes below it. Corpus-based analysis helps us to see the words mainly used by measuring their frequencies in the book of medicine. Therefore, this researcher suggests that the results of this analysis can be used for education of Chinese characters at the college of Korean Medicine.

• The Journal of Pediatrics of Korean Medicine
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• v.21 no.1
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• pp.155-171
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• 2007

Objectives : The aim of this study was to investigate the treatment of failure to thrive in current Traditional Chinese Medical Journals. Methods : To figure out the failure to thrive, Chinese Medical Journals from China National Knowledge Infrastructure(CNKI) which was published in 1994 to 2005 were analyzed. 矮(小)-whae(so) was searched. Results : Eleven articles were selected. 5 of them were used herbal medicine, and were showed that the total improvement rate was higher than 70% in failure to thrive. 2 of them were used acupuncture, and the last of them were used a therapy of combining Traditional Chinese Medicine with western medicine. Conclusions : The Oriental medical treatments for failure to thrive were presumed to be effective in most articles. But we think that the more studies are needed.

• Korean Institute of Interior Design Journal
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• no.18
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• pp.74-80
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• 1999

Chinese medicine hospital enables the users to expect more efficient cure result in the psychological stability and the comfortable indoor environment by supplying the functional and aesthetic cure space with the medical action of good quality Medical activity is to treat the human life. Thus, hospital must be more human-centered-place than other space. Thus study is establishment of space to be able to lead more rational and active participation than the conservative and passive image of the department of diagnosis and treatment for outpatients is desirable. This study is the indoor schedule to suggest the direction about the department of diagnosis and treatment for outpatients of Chinese medicine hospital of native image with more comfortable and positive approach on the basis of above points at issues as the schedule to fulfill the performance of medical function and the emotional and psychological satisfaction of users as the human being-centered-medical institution on the subject of the department of diagnosis and treatment for outpatients in Chinese medicine hospital. And, this researcher progressed as follows by being premised on this 1. Description of Goai, Range and Method of Study and Suggestion of Study Direction 2. Concept introduction as the Basic Approach of Theory which is necessary for Study 3. The Problems were recognized by grasping the present condition in Korea through the questionaines 4. establishment of Concept and direction which are necessary for planning the indoor of the department of Diagnosis and Treatment for Chinese Medicine Hospital 5. Progression of Design Plan attendant upon Concept 6. Analysis of the Conents attendant upon this, and Conclusiov.

• Journal of Korean Medical classics
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• v.26 no.1
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• pp.17-25
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• 2013

Objective : The main idea of this article is to investigate the specialization of Chinese traditional medicine. The ancient shaman played a bridge role between human beings and supernatural things like ghosts. Even though he didn't have the super power, the ancients believed that he could take care of all kinds of illness. Therefore, it can be said that the medicine of Zhou Dynasty was still under the shamanism although it had already started to be specialized and professionalized. And it was the important role of the ancient shaman of that period that gave patients the shamanistic treatment on the ground of the specialized medical knowledge, which was commonly activated during that time. Method : This article is going to look into the detailed aspects of the specialization of Chinese traditional medicine through the some kinds of written attestations of Zhou Dynasty. Result : The medical knowledge of that time stayed in the early stage, so it was simple and raw. Also it had scientific and unscientific characters in itself at the same time. That's why it could be included in the shamanism; the details can be offered through another article of mine entitled A Research of Shamanistic Medical Activities on Written Attestations in the Zhou Dynasty(兩周時期的醫療巫術), which is being written now. Conclusion : From beginning of the Eastern Zhou period, the medicine gradually got to be specialized. And then the specialized medical treatment and shamanistic medical activities began to be divided as the different two occupations. Searching for various written attestations of that period, we can see lots of records about the specialized medicine treatment such as acupuncture and moxibustion and the medical substances.

• Journal of Society of Preventive Korean Medicine
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• v.24 no.2
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• pp.1-15
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• 2020

Objectives : The objectives of this study were to investigate why and how China used traditional Chinese medicine as a response to COVID-19 and how its performance was achieved, and to explore ways to utilize traditional Korean medicine in Korea. Methods : We examined the information through government data and media articles. China's COVID-19 progress and policy response were reviewed and compared with Korea. Based on this, the characteristics of traditional Chinese medicine response in China were identified. Results : Based on legal basis, China makes the overall use of traditional Chinese medicines to respond to COVID-19. Traditional Chinese medicine has been applied to health insurance, the licensing regulations have been eased, and traditional Chinese medical specialists were dispatched. The medical care guidelines were developed and R&D were carried out. In addition, policies related to traditional Chinese medicine included policies for preventive treatment, the combination of Chinese and Western medicine, and telemedicine. Conclusions : Traditional Chinese medicine response to COVID-19 was included within the overall national quarantine policy, providing medical services for the mild stage. In addition, R&D was conducted to establish a basis for the utilization of traditional Chinese medicines. Traditional Korean medicine also needs to be prepared so that it can be used as a complement to the response of communicable diseases.

• Journal of Convergence for Information Technology
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• v.9 no.8
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• pp.102-109
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• 2019

Despite of advanced plans from Korean government to encourage Korean medical tourism, the medical tourism in Korea is still under competitive compared to the countries including Thailand, Singapore, and India, in which the government has been driving the development in industries of medical tourism, Moreover, the studies identifying the factors affecting tourists' satisfaction has not been actively performed up to date. Therefore, this study attempts to investigate the influences of quality of medical service, accessibility, cost adequacy, and quality of tourism of Korean medical tourism on Chinese tourists' satisfaction and revisit intention. For the purpose of empirical study, a survey has been conducted to Chinese medical tourists. Findings are as follows. First, the factors of medical tourism attributes including quality of medical service, cost adequacy, and quality of tourism have turned out to have positive effects on medical tourists' satisfaction. Second, tourists' satisfaction has a positive effect on revisit intention.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.14
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• pp.5815-5818
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• 2014

For an exact comparison of mRNA transcription in different samples or tissues with real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR), it is crucial to select a suitable internal reference gene. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and beta-actin (ACTB) have been frequently considered as house-keeping genes to normalize for changes in specific gene expression. However, it has been reported that these genes are unsuitable references in some cases, because their transcription is significantly variable under particular experimental conditions and among tissues. The present study was aimed to investigate which reference genes are most suitable for the study of gastric cancer tissues using qRT-PCR. 50 pairs of gastric cancer and corresponding peritumoral tissues were obtained from patients with gastric cancer. Absolute qRT-PCR was employed to detect the expression of GAPDH, ACTB, RPII and 18sRNA in the gastric cancer samples. Comparing gastric cancer with corresponding peritumoral tissues, GAPDH, ACTB and RPII were obviously upregulated 6.49, 5.0 and 3.68 fold, respectively. Yet 18sRNA had no obvious expression change in gastric cancer tissues and the corresponding peritumoral tissues. The expression of GAPDH, ${\beta}$-actin, RPII and 18sRNA showed no obvious changes in normal gastric epithelial cells compared with gastric cancer cell lines. The carcinoembryonic antigen (CEA), a widely used clinical tumor marker, was used as a validation gene. Only when 18sRNA was used as the normalizing gene was CEA obviously elevated in gastric cancer tissues compared with peritumoral tissues. Our data show that 18sRNA is stably expressed in gastric cancer samples and corresponding peritumoral tissues. These observations confirm that there is no universal reference gene and underline the importance of specific optimization of potential reference genes for any experimental condition.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.1
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• pp.39-49
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• 2004

This article will introduce the changes of the historiography of the Chinese medicine which have been made during the last several decades. These will be described with the changes in the image of the Chinese medicine that has been occupied in our mind. We regard Chinese medicine as a rational knowledge system which is based on the theory of Yin-Yang and Five phases. Therefore we draw a dear line of demarcation between Chinese medicine and irrational cure means such as religious prayer, charms and folk remedies which have no theoretical background. We also think the medical lineages continue virtually uninterrupted over the whole time of the history of Chinese medicine that began from Huangdineijing(皇帝內徑) and Shanghanlun(傷寒論). And we consider Chinese medicine as a sort of science, and suppose there has been one universal and homogeneous medical system in the mainland China. This image, however, has been changing rapidly. These changes come from the join of externalists who were trained as historian not physician, for studying the history of Chinese medicine, and the methodological innovations of historiography which was influenced by the methodologies of anthropology, social history, cultural history and the discourse of postmodemism.