• Parasites, Hosts and Diseases
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• v.38 no.2
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• pp.95-97
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• 2000

A 17 kDa protein from Clonorchis sinensis adults was purified by a procedure including Sephacryl S-200 HR gel filtration and Q-Sepharose anion exchange chromatography. The protein was proved to be a cysteine protease as it showed hydrolytic activity toward Cbz-Phe-Arg-AMC in the presence of dithiothreitol and was inhibited by specific inhibitors such as iodoacetic acid or trans epoxy-succinly-L-leucyl-amido(4 guanidino) butane. The polyclonal antibody raised against the protein reacted to 17 kDa proteins of trematodes such as Paragonimuf westermani, Fasciola hepatica, Opisthorchis viverrini, Gymnophalloides seoi, and Metagonimus yokogawai. The antibody recognized the 17 kDa and 16 kDa cysteine proteases purified from C. sinensis, P. westemani, and G. seoi as well. These results suggest that the 17 kDa protein may be a cysteine protease commonly present in trematodes.

• Journal of Korean Neurosurgical Society
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• v.29 no.6
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• pp.822-825
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• 2000

Lymphocytic infundibuloneurohypophysitis is a rare inflammatory disorder, which may be caused by autoimmune response, affecting the infundibulum and the posterior lobe of the pituitary gland. The authors present a 15-year-old man with central diabetes insipidus, whose sellar magnetic resonance imaging (MRI) showed a focal thickening of the infundibulum and loss of high signal of the normal neurohypophysis on T1-weighted images. The histopathological diagnosis was lymphocytic infundibuloneurohypophysitis. When a patient presents with central diabetes insipidus and infundibular thickening on brain MRI, this entity should be included in the differential diagnosis though it is very rare. The significance of this entity in treatment is that it is basically not a surgical one, and surgery is needed only for tissue diagnosis. Symptomatic treatment and observation make it regress spontaneously.

• The Bulletin of The Korean Astronomical Society
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• v.45 no.1
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• pp.34.3-34.3
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• 2020

Faint z ~ 5 quasars with M1450 ~ -23 mag are known to be the potentially important contributors to the ultraviolet ionizing background in the post-reionization era. However, their number density has not been well determined, making it difficult to assess their role in the early ionization of the intergalactic medium (IGM). In this work, we present the updated results of our z ~ 5 quasar survey using the Infrared Medium-deep Survey (IMS), a near-infrared imaging survey covering an area of 85 square degrees. From our spectroscopic observations with the Gemini Multi-Object Spectrograph (GMOS) on the Gemini-South 8 m Telescope, we discovered eight new quasars at z ~ 5 with -26.1 ≤ M1450 ≤ -23.3. Combining our IMS faint quasars with the brighter Sloan Digital Sky Survey (SDSS) quasars, we derive, for the first time, the z ~ 5 quasar luminosity function (QLF) without any fixed parameters down to the magnitude limit of M1450 = -23 mag. We find that the faint-end slope of the QLF is very flat (-1.2) with a characteristic luminosity of -25.7 mag. The number density of z ~ 5 quasars from the QLF gives lower ionizing emissivity and ionizing photon density than those in previous works. These results imply that quasars are responsible for only 10-20% of the photons required to completely ionize the IGM at z ~ 5, disfavoring the idea that quasars alone could have ionized the IGM at z ~ 5.

• Parasites, Hosts and Diseases
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• v.38 no.1
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• pp.9-15
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• 2000

A scanning electron microscopic study was performed on the surface ultrastructure of metacercariae and adults of Metagonimus takahashii. Metacercariae were collected from the scale of crucian carp (Carassius auratus) , and adult flukes were harvested 1-4 weeks after infection to rats. In excysted metacercariae. the oral sucker had type I (numerous) and type II (seven in total) sensory papillae. Tegumental spines were dense and digitated into 5-7 points on the surface anterior to the ventral sucker, but became sparse and less digitated posteriorly toward the end of the body In adults, seven type II sensory papillae were characteristically arranged around the lip of the oral sucker, and on the inner side of the lip four small and two large type I sensory papillae were symmetrically seen on each side (12 in total). Tegumental spines on anterior two-thirds of the body. were digitated with 9-12 tips ventrally and 8-13 tips dorsally. Sperms entering into the Laurer's canal were observed. The results show that the surface ultrastructure of M. takuhashii is generally similar to those of M. yokogawai and M. miyatai except for the digitation of tegumental spines.

• Parasites, Hosts and Diseases
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• v.43 no.4 s.136
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• pp.129-134
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• 2005

To determine the status of Enterobius vermicularis infection among children living on western and southern coastal islands of the Republic of Korea, children (3-10 years) in 39 kindergartens and primary schools were examined using the cello-tape anal swab method, during July and August 2000. Of 1,661 children examined, $307(18.5\%) $were found to be positive for E. vermicularis eggs. The highest infection rate $(59.3\%)$ was found in a kindergarten and a branch school of Shinyang primary school on Chujado, Jeju-do (Province), Remarkable differences in egg positive rates were observed in different localities. The egg positive rate for boys $(21.3\%)$ was significantly higher than that of girls $(15.4\%)$ (P=0.02). However, positive rates were not significantly dependent on age. The results of the present study show that E. vermicularis infection is highly prevalent among pre-school and primary school children living on the western and southern coastal islands of the Republic of Korea, and indicate the need for efforts to control this infection.

• Korean Journal of Microbiology
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• v.30 no.4
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• pp.278-285
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• 1992

Chemotaxonomic and numerical identification were carried out for a isolate of Streptomyces strain SMF301 producing spores in submerged culture. Fifty taxonomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isolate SMF301 was identified to cluster 1A of Streptomyces and best matched to Streptomyces limosus which is a synonym of Streptomyces albidoflavus. Therefore, it was concluded that the isolate was identified to be a member of Streptomyces alidoflavus.

• Nutrition Research and Practice
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• v.12 no.2
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• pp.173-173
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• 2018

• 한국균학회소식:학술대회논문집
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• 2014.10a
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• pp.9-9
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• 2014

Null mutants generated by targeted gene replacement are frequently used to reveal function of the genes in fungi. However, targeted gene deletions may be difficult to obtain or it may not be applicable, such as in the case of redundant or lethal genes. Constitutive expression system could be an alternative to avoid these difficulties and to provide new platform in fungal functional genomics research. Here we developed a novel platform for functional analysis genes in Magnaporthe oryzae by constitutive expression under a strong promoter. Employing a binary vector (pGOF1), carrying $EF1{\beta}$ promoter, we generated a total of 4,432 transformants by Agrobacterium tumefaciens-mediated transformation. We have analyzed a subset of 54 transformants that have the vector inserted in the promoter region of individual genes, at distances ranging from 44 to 1,479 bp. These transformants showed increased transcript levels of the genes that are found immediately adjacent to the vector, compared to those of wild type. Ten transformants showed higher levels of expression relative to the wild type not only in mycelial stage but also during infection-related development. Two transformants that T-DNA was inserted in the promotor regions of putative lethal genes, MoRPT4 and MoDBP5, showed decreased conidiation and pathogenicity, respectively. We also characterized two transformants that T-DNA was inserted in functionally redundant genes encoding alpha-glucosidase and alpha-mannosidase. These transformants also showed decreased mycelial growth and pathogenicity, implying successful application of this platform in functional analysis of the genes. Our data also demonstrated that comparative phenotypic analysis under over-expression and suppression of gene expression could prove a highly efficient system for functional analysis of the genes. Our over-expressed transformants library would be a valuable resource for functional characterization of the redundant or lethal genes in M. oryzae and this system may be applicable in other fungi.

• Proceedings of the Korean Magnestics Society Conference
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• 2010.06a
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• pp.112-113
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• 2010

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2002.04b
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• pp.154-154
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• 2002