• Genomics & Informatics
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• 제6권2호
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• pp.64-67
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• 2008

The Korean Rice Ds-tagging lines Database (KRDD) is designed to provide information about Ac/Ds insertion lines and activation tagging lines using japonica rice. This database has provided information on 18,158 Ds lines, which includes the ID, description, photo image, sequence information, and gene characteristics. The KRDD is visualized using a web-based graphical view, and anonymous users can query and browse the data using the search function. It has four major menus of web pages: (i) a Blast Search menu of a mutant line; Blast from rice Ds-tagging mutant lines; (ii) a primer design tool to identify genotypes of Ds insertion lines; (iii) a Phenotype menu for Ds lines, searching by identification name and phenotype characteristics; and (iv) a Management menu for Ds lines.

• IMMUNE NETWORK
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• 제8권2호
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• pp.53-58
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• 2008

Background: Molecular mechanisms of natural killer (NK) cell development from hematopoietic stem cells (HSCs) have not been clearly elucidated, although the roles of some genes in NK cell development have been reported previously. Thus, searching for molecules and genes related NK cell developmental stage is important to understand the molecular events of NK cell development. Methods: From our previous SAGE data-base, Gpnmb (Glycoprotein non-metastatic melanoma protein B) was selected for further analysis. We confirmed the level of mRNA and protein of Gpnmb through RT-PCR, quantitative PCR, and FACS analysis. Then we performed cell-based ELISA and FACS analysis, to know whether there are some molecules which can bind to Gpnmb. Using neutralizing antibody, we blocked the interaction between NK cells and OP9 cells, and checked IFN-${\gamma}$ production by ELISA kit. Results: Gpnmb expression was elevated during in vitro developmental stage and bound to OP9 cells, but not to NK precursor cells. In addition, we confirmed that the levels of Gpnmb were increased at NK precursor stage in vivo. We confirmed syndecan4 as a candidate of Gpnmb's binding molecule. When the interaction between NK cells and OP9 cells were inhibited in vitro, IFN-${\gamma}$ production from NK cells were reduced. Conclusion: Based on these observations, it is concluded that Gpnmb has a potential role in NK cell development from HSCs.

• 한국정보과학회논문지:정보통신
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• 제29권2호
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• pp.165-173
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• 2002

In the static channel assignment scheme for cellular mobile computing environment, each cell has a fixed number of channels and supports a service for a user′s request entering to the cell. This scheme has an advantage of simplicity. However, this scheme has a disadvantage that can′t control far hot cell problem. Therefore, to solve this problem, the "channel borrowing" concept is needed. In this paper, we propose a load balancing(channel borrowing, channel reassignment) approach using genetic algorithm. The purposes of using genetic algorithm in this paper are ${\circled1}$ to find early a cell including an available channel and ${\circled2}$ to decrease a number of probings and ${\circled3}$ to migrate to the cell after searching an available channel near upon optimality. To represent effectiveness of the proposed algorithm, we simulated various experiments.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2000년도 봄 학술발표회 논문집
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• pp.685-692
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• 2000

Vertical drain used improvement soft clay is made of not only decreasing construction time but also increasing the ground strength during some decades. As, it is applied to improvement soft clay with vertical drain, it is designed by the result that is caused by oedemeter test ignored anisotropic of the ground related to consolidation conditions. When we are expected consolidation conditions, the most important factors is soil of compaction and water permeability. Above all, anisotropic of the ground permeability show the results which differ between vertical and radial drainage. Recently, We study for radial consolidation coefficient and permeability coefficient that utilized Rowe Cell Consolidation and permeability tester but, it dont use well because of not only a supply lack also difficulty of test. The paper experimented with searching anisotropic of the ground so there are Rowe Cell test, standard consolidation tester and modified standard consolidation test that have pohang's soft clay ground. Therefore, we find anisotropic of the ground and a tester of easy use more than before. We made a comparison test result between the devised tester and Rowe Cell tester, Also, we learned average degree of consolidation for partial penetrating vertical drains. We were found relations as effective stress-void and effective stress-permeability coefficient through those tests.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2008년도 International Symposium on Remote Sensing
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• pp.274-276
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• 2008

At the stage of content convergence into cell phone, technologies for geo-spatial information sharing and searching are being developed. Currently, 2D portable navigation map for mobile navigation is provided by communication companies, but geobrowers for 3D geo-information in cell phone are under developing. In this study, 3D feature transformation among X3D-M3G-KML, on mobile and web environments, is dealt with as the first stage for the further mobile 3D web application. As well, it is possible to real-time interoperable 3D geo-information exchange issues within both environments.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1995년도 춘계학술대회
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• pp.49-53
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• 1995

In this paper, we classify between the chromosome and blood cell, and find the location of chromosome. First, the gray level images be the binary images using the threshold method. Then, the spot noises are removed by the morphological filtering. Features are obtained using the updated Run length(RL) coding and are classified using the Bayes decision rule. The performances of classification are 83.3% in chromosome and 93.3% in blood cell. Because each sub-images ($256{\times}256$) is obtained from the full image($512{\times}512$), we realize the location of chromosome if we get the corrected chromosome classifications.

• Asian Pacific Journal of Cancer Prevention
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• 제17권5호
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• pp.2499-2506
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• 2016

Background: Gastric cancer (GC) is the second leading cause of cancer-related death worldwide. Several environmental, genetic and epigenetic factors have been suggested to have a role in GC development. Epigenetic mechanisms like histone changes and promoter hyper-methylation are now being increasingly studied. Associations between methylation of many gene promoters with the risk of gastric cancer have been investigated worldwide. Such aberrant methylation may result in silencing of specific genes related to cell cycling, cell adhesion, apoptosis and DNA repair. Thus this molecular mechanism might have a key role in proliferation and migration of cancerous cells. Materials and Methods: In this review article we included studies conducted on DNA methylation and gastric cancer in Iranian populations. Using Science direct, Pubmed/PMC, Springer, Wiley online library and SciELO databases, all published data until 31 January 2016 were gathered. We also searched Science direct data base for similar investigations around the world to make a comparison between Iran and other countries. Results: By searching these databases, we found that the association between methylation of seven gene promoters and gastric cancer had been studied in Iran until 31 January 2016. These genes were p16, hLMH1, E-cadherin, CTLA4, $THR{\beta}$, mir9 and APC. Searching in science direct database also showed that 92 articles had been published around the world till January 2016. Our investigation revealed that despite the importance of GC and its high prevalence in Iran, the methylation status of only a few gene promoters has been studied so far. More studies with higher sample numbers are needed to reveal the relation of methylation status of gene promoters to gastric cancer in Iran. Conclusions: Further studies will be helpful in identifying associations of DNA methylation in candidate genes with gastric cancer risk in Iranian populations.

• 전자공학회논문지C
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• 제36C권10호
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• pp.8-16
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• 1999

본 연구에서는 시뮬레이션 효율을 향상시킨 시뮬레이션 기반의 아날로그 셀 합성기법을 제안한다. 아날로그 셀을 계층적으로 합성하기 위하여 시뮬레이션 기반으로 전류미러, 차동입력단 등 각각의 부회로(sub circuit) 생성기들을 개발하였다. 이 부회로 생성기들을 모듈화 시키고 계층화시킴으로써 OTA(operational transconductance amplifier)나 2단(2-stage) OP-AMP, 비교기(comparator)등 일반적인 아날로그 셀들의 합성을 위하여 사용될 수 있게 하였다. 시뮬레이션 기반의 합성 시간을 줄이기 위하여 2단계 탐색 기법 (2-stage searching scheme)과 시뮬레이션 데이터 재사용기법(simulation data reusing scheme)을 제안하여 적용하였다 아날로그 셀(OTA) 합성 시 301.05sec에서 56.52sec로 최고 81.2%의 합성 시간을 줄이므로 시뮬레이션 기반의 회로 합성시 긴 합성시간의 문제를 해결하였다. 개발한 합성기는 SPICE의 모델 파라미터외에 추가적인 물리적 파라미터들을 필요로 하지 않으며 공정이나 SPICE 모델 레벨(level)에 독립적이기 때문에 새로운 공정에 적용할 때 필요한 준비 시간이 최소화되었다. 본 논문에서는 OTA와 2단 OP-AMP를 각각 합성하여 제안하는 합성기법의 유용성을 입증하였다.

• Journal of the Korean Wood Science and Technology
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• 제18권2호
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• pp.49-66
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• 1990

In order to identify an unknown wood sample native to Korea. the softwood databases(KSWCHUN; Korean SoftWood CHUN) and the hardwood databases(KHWCHUN; Korean HardWood CHUN) had been built. and the new computer searching programs(IDINEX; IDentification INformation EXpress) has been written in Turbo Pascal(V.5.0) and in Macro Assembly(V.5.0). The characters of the data were based on the 74 features of softwood and on the 148 features of hardwood which are a part of new "IAWA list of microscopic features for hardwood identification" published in 1989. For the purpose of this investigation the wood anatomical nature of 25 species of softwood(13 genera of 5 families) and of 112 species of hardwood(57 genera of 31 families) were observed under a scanning electron microscope and light microscope. and a lot of literature used. The IDINEX programs are based on edge-punched card keys. with several improvements. The maximum number of features in the IDINEX is 229. but that is fixed for a given database. Large numbers of taxa are handled efficiently and new taxa easily added. A search may be based on sequence numbers of features. Comparisons are made sequentially by feature and taxon using the entire suite of features specified to produce the list of possible matching taxa. The results are followings. (1) The databases of Korean wood and the searching programs(IDINEX) had been built. (2) The databases of Korean wood could be an information to search an unknown wood. (3) The databases would be valuable. for the new features, which were not mentioned in Korean wood up to the present. were observed in details. (4) The ultrastructures of the cell walls(warty layer) and crystals observed under a scanning electron microscope will be helpful to search an unknown wood in particular. (5) The searching process is more quick and accurate than the others. 6) We can obtain the information on the differences of a species from the other and search an unknown wood using probability. in IDINEX, (7) The IDINEX will be utilized to identify and classify an animal life, vegetable world, mineral kingdom, and so on.

• IMMUNE NETWORK
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• 제8권3호
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• pp.98-105
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• 2008

Background: Allergic inflammation was induced by activated Th2 lymphocytes, leading to IgE production and eosinophil activation. A Th2 disproportion was shown in atopic children soon after birth. During specific allergen stimulation, an increase of Th2 cells was observed in most cases. In this study, we prepared new screening "whole blood" system for searching the anti-atopic materials. Cytokine production and IgE secretion from whole blood system were assessed and we confirmed the results by using animal system. Methods: Pathological features in NC/Nga mice are similar to those observed in human atopic dermatitis. Whole blood from NC/Nga mouse was stimulated by using TNCB (Th2 activator) or candidate materials of anti-atopic dermatitis, and the production of cytokines (IL-4, IL-12, and IFN-${\gamma}$) were measured by ELISA. In order to confirm the results of whole blood system, in vivo test was done by using NC/Nga mice. Results: In whole blood system, LPS and extracts of green tea, hardy orange and onion induced the production of IL-12 and IFN-${\gamma}$ while they reduced the production of IL-4. Also, LPS and extracts of onion reduced IgE production. Though atopic dermatitis was observed from a mouse stimulated with TNCB, it was not when a mouse was co-stimulated in LPS or extracts of onion. The results are same as those observed in whole blood system. Conclusion: Whole blood system was simple and speedy methods for searching a materials compared with the conventional high-cost animal system. And the results using whole blood system was proved to be reliable in our experiments for screening anti-atopic material. We expect that the system can be applied to other experiments for searching similar materials.