• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2003년도 제2차 연례학술대회 발표논문집
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• pp.251-257
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• 2003

Metabolic engineering has become a new paradigm for the more efficient production of desired bioproducts. Metabolic engineering can be defined as directed modification of cellular metabolism and properties through the introduction, deletion, and modification of metabolic pathways by using recombinant DNA and other molecular biological tools. During the last decade, metabolic flux analysis(MFA) has become an essential tool fur metabolic engineering. By MFA, the intracellular metabolic fluxes can be quantified by the measurement of extracellular metabolite concentrations in combination with the stoichiometry of intracellular reactions and mass balances. The usefulness and functionality of MFA are demonstrated by applying to metabolic pathways in E. coli. First, a large-scale in silico E. coli model is constructed, and then the effects of carbon sources on intracellular flux distributions and succinic acid production were investigated on the basis of the uptake and secretion rates of the relevant metabolites. The results indicated that succinic acid yields increased in order of gluconate, glucose and sorbitol. Acetic acid and lactic acid were produced as major products rather than when gluconate and glucose were used carbon sources. The results indicated that among three carbon sources available, the most reduced substrate is sorbitol which yields efficient succinic acid production.

• Composites Research
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• 제33권4호
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• pp.205-212
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• 2020

본 연구에서는 기상화학반응공정을 이용하여 실리콘카바이드가 코팅된 2종형상의 탄소/탄소복합재 위에 진공플라즈마용사를이용하여 하프늄카바이드를 코팅하였다. 코팅 전/후 시편을 5.06 MW/㎡의 열유속에서 120초 동안 산화 및 삭마 실험을 진행하였다. 시험 전/후의 질량 변화량을 통해 질량삭마율을 계산하였고 캘리퍼스와 고속카메라를 이용하여 길이변화를 측정하여 길이삭마량을 계산하였다. 시편 단면의 FE-SEM 및 EDS 분석을 통해 산화 및 삭마거동을 관찰하였다. 플라즈마 풍동 시험결과 코팅된 시편이 무게감소 및 길이변화가 적어 내산화 및 내삭마성이 높은 것으로 판단되었다. 그러나 동일조건에서 시험한 반구형과 원통형의 산화 및 삭마정도는 상이하였고 원통형에서 더 높은 내산화 및 내삭마성을 가지는 것으로 평가되었다.

• 한국농림기상학회지
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• 제5권2호
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• pp.138-149
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• 2003

KoFlux는 생태계와 대기 사이에 교환되는 이산화탄소, 수증기 및 에너지에 대한 우리의 이해를 높이고, FLUXNET과 CEOP등의 지역, 대륙 및 전구 규모의 관측망에 기여하기 위해서 시작되었다. 그거나 한반도의 지형적 특성 때문에 KoFlux의 대부분의 플럭스 타워는 관측에 이상적이지 못한 장소에 위치하고 있다. 탄소 및 에너지 교환의 정량화를 위해서 뿐만 아니라 군락 규모에서 지역 규모로 확장하기 위해서는 관측과 모델링을 병용한 다양한 접근 방법의 적용이 필요하다. 본 연구에서는 지역 규모의 현열 플럭스를 추정하기 위해 타워 플럭스 관측, 대류 경계층(CBL) 수지 방법, MM5 중규모 모형, 그리고 NCAR/NCEP 재분석 자료의 네 가지의 방법을 사용하여 다양한 면적을 대표하는 현열 플럭스를 산출하여 비교하였다. 비록 제한된 짧은 기간의 자료를 사용하였으나, 예비 분석을 통하여 (1) 해남 농경지 플럭스 타워에서 관측된 현열 플럭스가 지표의 불균질성을 보였고, (2) CBL 수지 방법으로 얻어진 지역 규모의 현열 플럭스는 수평 이류 효과의 계산 방법에 따라 다른 결과를 보였으며, (3) MM5 중규모 모형은 타워 플럭스 관측 값과 아주 유사한 현열 값을 수치 모사하였다. 그러나 관측지의 불균질성과 두 방법이 대표하는 면적의 근본적인 차이를 고려할 때, 플럭스 발자국 분석, 지리정보 시스템 및 관측지의 위성 영상 분석에 근거한 타워 플럭스의 공간 대표성을 정량화하는 것이 시급한 것으로 나타났다.

• 비파괴검사학회지
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• 제21권5호
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• pp.485-492
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• 2001

본 논문에서는 누설자속탐상에 대한 전자기 수치해석을 수행하였다. 수치해석방법으로 2차원 유한요소법을 이용하였다. 자기벡터포텐셜을 미지수호 사용하였으며, 자기비선형(磁氣非線型)을 고려한 자장해석을 수행하여 자기포화에 의한 영향을 고려하였다. 수치해석 결과를 검증하기 위하여 실험장치를 자체 제작하여 실험을 수행하였다 시료로는 SM 45C 탄소강을 사용하였으며, 표면에 몇 가지 결함을 생성하였다. 시편과 실험장치를 이용하여 결함을 검출하는 비파괴검사를 수행하였다. 결함의 깊이 변화 및 결함의 형상변화에 대하여 결과를 비교 검토하였다. 수치해석 및 실험에서 유사한 결과를 얻었으며, 수치해석을 통하여 누설자속탐상의 시뮬레이션이 가능함을 보였다.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2004년도 춘계학술대회 논문집
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• pp.277-283
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• 2004

The time to ignition, heat release rate characteristics and carbon monoxide yield of fiber reinforced and sandwich phenol resin were investigated with cone calorimeter. The fire characteristics of unsaturated polyester, mostly being applied to the existing passenger train, and phenolic resin were compared. Thermal gravimetric analysis(TGA) was used to monitor the degree of thermal decomposition for the phenolic resin. According to the cone calorimeter data, the time to ignition, heat release rate and CO yield was faster and higher as the external heat flux increase. Under the same heat flux, the time to ignition of sandwich type phenolic resin was shorter than that of fiber reinforced. The result of comparison between unsaturated polyester and phenolic resin was that phenolic resin was shown to have better fire resistance than that of unsaturated polyester.

• 설비공학논문집
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• 제13권6호
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• pp.462-473
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• 2001

Due to the coupling between momentum and energy transport theoretical analysis of the loop performance is very complicate, therefore it is necessary that these problems be solved by experimental investigation before applying th loop thermosyphon to heat exchanger design. The evaporator and condenser of the loop thermosyphon were made of carbon-steel, and distilled water was used as working fluid in the experiments. From the experimental data correlations of heat transfer coefficient for evaporator and condenser sections were obtained. For heat fluxes in th range of 13~78kW/$m^2$, the correlation equations of heat transfer coefficients in evaporator and condenser predict the experimental behavior to within $\p$\pm$5% and\;\pm20$% respectively.

• Current Research on Agriculture and Life Sciences
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• 제32권4호
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• pp.199-202
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• 2014

The bioconversion of cellulosic biomass hydrolyzates consisting mainly of glucose and xylose requires the use of engineered Saccharomyces cerevisiae expressing a heterologous xylose pathway. However, there is concern that a fungal xylose pathway consisting of NADPH-specific xylose reductase (XR) and $NAD^+$-specific xylitol dehydrogenase (XDH) may result in a cellular redox imbalance. However, the glycerol biosynthesis and glycerol degradation pathways of S. cerevisiae, termed here as the glycerol cycle, has the potential to balance the cofactor requirements for xylose metabolism, as it produces NADPH by consuming NADH at the expense of one mole of ATP. Therefore, this study tested if the glycerol cycle could improve the xylose metabolism of engineered S. cerevisiae by cofactor balancing, as predicted by an in-silico analysis using elementary flux mode (EFM). When the GPD1 gene, the first step of the glycerol cycle, was overexpressed in the XR/XDH-expressing S. cerevisiae, the glycerol production significantly increased, while the xylitol and ethanol yields became negligible. The reduced xylitol yield suggests that enough $NAD^+$ was supplied for XDH by the glycerol cycle. However, the GPD1 overexpression completely shifted the carbon flux from ethanol to glycerol. Thus, moderate expression of GPD1 may be necessary to achieve improved ethanol production through the cofactor balancing.

• International Journal of Air-Conditioning and Refrigeration
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• 제10권3호
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• pp.153-161
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• 2002

Due to the coupling between momentum and energy transport theoretical analysis of the loop performance is very complicate, therefore it is necessary that these problems be solved by experimental investigation before applying the loop thermosyphon to heat exchanger de-sign. The evaporator and condenser of the loop thermosyphon were made of carbon-steel, and distilled water was used as working fluid in the experiments. From the experimental data correlations of heat transfer coefficient for evaporator and condenser sections were obtained. For heat fluxes in the range of 13000~78000 W/$m^2$, the correlation equations of heat transfer coefficients in evaporator and condenser predict the experimental behavior to within $\pm$5% and $\pm$20% respectively.

• Coupled systems mechanics
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• 제12권1호
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• pp.21-40
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• 2023

In thisstudy, the vibration problem ofthermo elastic carbon nanotubes conveying pulsating viscous nano fluid subjected to a longitudinal magnetic field is investigated via Euler-Bernoulli beam model. The controlling partial differential equation of motion is arrived by adopting Eringen's non local theory. The instability domain and pulsation frequency of the CNT is obtained through the Galerkin's method. The numerical evaluation of thisstudy is devised by Haar wavelet method (HWM). Then, the proposed model is validated by analyzing the critical buckling load computed in presentstudy with the literature. Finally, the numerical calculation ofsystem parameters are shown as dispersion graphs and tables over non local parameter, magnetic flux, temperature difference, Knudsen number and viscous parameter.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권5호
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• pp.281-288
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• 2002

Recombinant Escherichia coli strain harboring the ${\lambda}$pR-pL promotor and heterologus poly-${\beta}$-hydroxybutyrate (PHB) biosynthesis genes was used to investigate the effect of culture conditions on the efficient PHB production. The expression of phb genes was induced by a temperature upshift from $33^{\circ}C\;to\;38^{\circ}C$. The protein expression levels were measured by using two-dimensional electrophoresis, and the enzyme activities were also measured to understand the effect of culture temperature, carbon sources, and the dissolved oxygen (DO) concentration on the metabolic regulations. AcetylCoA is an important branch point for PHB production. The decrease in DO concentration lowers the citrate synthase activity, thus limit the flux toward the TCA cycle, and increase the flux for PHB production. Since NADPH is required for PHB production, the PHB production does not continue leading the overproduction of acetate and lac-tate. Based on these observations, a new operation was considered where DO concentration was changed periodically, and it was verified its usefulness for the efficient PHB production by experiments.