• 제목/요약/키워드: carbon flux

검색결과 463건 처리시간 0.03초

Roles of Glucose and Acetate as Carbon Sources in L-Histidine Production with Brevibacterium flavum FERM1564 Revealed by Metabolic Flux Analysis

  • Shioya, Suteaki;Shimizu, Hiroshi;Shimizu, Nobuyuki
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제7권3호
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    • pp.171-177
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    • 2002
  • The metabolic flux pattern for L-histidine production was analyzed when glucose and/or acetate were used as carbon sources. Total L-histidine production was enhanced when mixed substrate (glucose and acetate) was used, compared wish that when either glucose or acetate was used as the sole carbon source. Theoretical maximum carbon fluxes through the main pathways for L-histldine production, cell growth, and ATP consumption for cell maintenance were obtained by the linear programming (LP) method. By comparison of the theoretical maximum carbon fluxes tilth actual ones, it was found that a large amount of glucose was actually used for maintenance of cell viability. On the other hand, acetate was used for cell growth. After depletion of acetate in the mixed substrate culture, the flux for glucose to L-histldine synthesis was markedly enhanced. A strategy for effective L-histidine production using both carbon sources was proposed.

Mapping of Carbon Flow Distribution in the Central Metabolic Pathways of Clostridium cellulolyticum: Direct Comparison of Bacterial Metabolism with a Soluble versus an Insoluble Carbon Source

  • DESVAUX, MICKAEL,
    • Journal of Microbiology and Biotechnology
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    • 제14권6호
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    • pp.1200-1210
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    • 2004
  • Metabolic flux analysis was established by adapting previous stoichiometric model developed during growth with cellulose to cell grown with cellobiose for further direct comparison of the bacterial metabolism. In carbon limitation with cellobiose, a shift from acetate-ethanol fermentation to ethanol-lactate fermentation is observed and the pyruvate overflow is much higher than with cellulose. In nitrogen limitation with cellobiose, the cellodextrin and exopolysaccharide overflows are much higher than on cellulose. In carbon and nitrogen saturation with cellobiose, the cellodextrin, exopolysaccharide, and free amino acids overflows reach the highest levels observed but all remain limited on cellulose. By completely shunting the cellulosome, the use of cellobiose allows to reach much higher carbon consumption rates which, in return, highlights the metabolic limitation of C. cellulolyticum. Therefore, the physical nature of the carbon source has a profound impact on the metabolism of C. cellulolyticum and most probably of other cellulolytic bacteria. For cellulolytic bacteria, the use of soluble carbon substrate must carefully be taken into consideration for the interpretation of results. Direct comparison of metabolic flux analysis from cellobiose and cellulose revealed the importance of cellulosome, phosphoglucomutase and pyruvate-ferredoxin oxidoreductase in the distribution of carbon flow in the central metabolism. In the light of these findings, future directions for improvement of cellulose catabolism by this bacterium are discussed.

기후변동성에 따른 육상 탄소 순환의 반응 (Response of the Terrestrial Carbon Exchange to the Climate Variability)

  • 선민아;조천호;김영미;이조한;부경온;변영화
    • 대기
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    • 제27권2호
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    • pp.163-175
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    • 2017
  • The global terrestrial ecosystems have shown a large spatial variability in recent decades and represented a carbon sink pattern at mid-to-high latitude in Northern Hemisphere. However, there are many uncertainties in magnitude and spatial distribution of terrestrial carbon fluxes due to the effect of climate factors. So, it needs to accurately understand the spatio-temporal variations on carbon exchange flux with climate. This study focused on the effects of climate factors, .i.e. temperature, precipitation, and solar radiation, to terrestrial biosphere carbon flux. We used the terrestrial carbon flux that is simulated by a CarbonTracker, which performs data assimilation of global atmospheric $CO_2$ mole fraction measurements. We demonstrated significant interactions between Net Ecosystem Production (NEP) and climate factors by using the partial correlation analysis. NEP showed positive correlation with temperature at mid-to-high latitude in Northern Hemisphere but showed negative correlation pattern at $0-30^{\circ}N$. Also, NEP represented mostly negative correlation with precipitation at $60^{\circ}S-30^{\circ}N$. Solar radiation affected NEP positively at all latitudes and percentage of positive correlation at tropical regions was relatively lower than other latitudes. Spring and summer warming had potentially positive effect on NEP in Northern Hemisphere. On the other hand as increasing the temperature in autumn, NEP was largely reduced in most northern terrestrial ecosystems. The NEP variability that depends on climate factors also differently represented with the type of vegetation. Especially in crop regions, land carbon sinks had positive correlation with temperature but showed negative correlation with precipitation.

The Partitioning of Organic Carbon Cycle in Coastal Sediments of Kwangyang Bay

  • Han, Myung-Woo;Lee, In-Ho;Kim, Kee-Hyun;Noh, Il
    • Journal of the korean society of oceanography
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    • 제32권3호
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    • pp.103-111
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    • 1997
  • Biogeochemical cycling of organic carbon is quantitatively partitioned in terms of 1) flux to the ocean bottom, 2) benthic utilization at or near the sediment-water interface, 3) remineralization and 4) burial within sediments, by making an independent determination for each component process from a single coastal site in Kwangyang Bay. The partitioning suggests that the benthic utilization at or near the sediment-water interface is the major mode of organic carbon cycling at the site. The benthic utilization takes 61.8% (441.6 gCm$^{-2}$ yr $^{-1}$) of the total near-bottem organic carbon flux, 714.6 gCm $^{-2}$yr$^{-1}$, and far exceeds the remineralization of organic carbon within the sediments which amounts only to 6% (41.24 gCm$^{-2}$yr$^{-1}$) of the total near-bottom flux. The residence time is about 1.6 years for the sedimentary metabolic organic carbon in the upper 45 cm. The dominant partitioning of the benthic utilization in the carbon budget suggests that most of labile organic carbons are consumed at or near the sediment-water interface and are left over to the sediment column by significantly diminished amounts.

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탄소나노튜브 및 그래핀 나노유체 사용시 장기운전에 따른 열전달성능 변화에 대한 연구 (Study on Heat Transfer Performance Change According to Long-term Operation Using Carbon Nanotube and Graphene Nanofluid)

  • 김영훈;김남진
    • 한국태양에너지학회 논문집
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    • 제37권1호
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    • pp.15-23
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    • 2017
  • Critical heat flux refers to the sudden decrease in boiling heat transfer coefficient between a heated surface and fluid, which occurs when the phase of the fluid near the heated surface changes from liquid to vapor. For this reason, critical heat flux is an important factor for determining the maximum limit and safety of a boiling heat transfer. Recently, it is reported that the nanofluid is used as a working fluid for the critical heat flux enhancement. However, it could be occurred nano-flouling phenomena on the heat transfer surface due to nanoparticles deposition, when the nanofluid is applied in a heat transfer system. In this study, we experimentally carried out the effects of the nano-fouling phenomena in oxidized multi-wall carbon nanotube and oxidized graphene nanofluid systems. It was found that the boiling heat flux decreased by hourly 0.04 and $0.03kW/m^2$, also the boiling heat transfer coefficient decreased by hourly 11.56 and $10.72W/m^2{\cdot}K$, respectively, in the thermal fluid system using oxidized multi-wall carbon nanotube or oxidized graphene nanofluid.

함평만 무안 지역 갯벌의 식생 및 비식생 환경에서 이산화탄소 농도와 플럭스의 계절 변동 (Seasonal variations of CO2 concentration and flux in vegetation and non-vegetation environments on the Muan tidal flat of Hampyong Bay)

  • 소윤환;강동환;권병혁;김박사
    • 한국습지학회지
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    • 제21권4호
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    • pp.257-266
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    • 2019
  • 본 연구에서는 함평만에 속하는 무안 지역의 갯벌에서 갈대가 서식하는 식생 지역과 갈대가 없는 비식생 지역을 6개 지점씩 선정하여 저조시에 계절별로 이산화탄소 농도와 플럭스 및 지온의 변화를 관측하였다. 관측은 2012년 5월 30일(봄)과 8월 8일(여름), 2013년 1월 31일(겨울)에 각각 수행되었다. 관측 결과, 식생 지역의 이산화탄소 평균농도는 전체적으로 겨울에 가장 높았고 봄, 여름 순으로 나타났으며, 비식생 지역도 식생 지역과 같은 농도변화를 보였다. 식생 지역의 이산화탄소 플럭스는 봄과 여름에 모두 양(+)의 값으로 방출(emission)의 형태를 보이나, 겨울에는 반대로 흡수(uptake)하는 음(-)의 값으로 나타났다. 이산화탄소 플럭스의 평균값은 봄이 가장 높았으나 여름과 거의 유사하였고, 겨울은 낮은 음의 값으로 나타났다. 비식생 지역은 봄에 양의 값으로 방출의 형태를 보였고, 여름과 겨울에는 흡수하는 음의 값으로 나타났다. 평균값은 봄이 가장 높았고, 여름과 겨울의 차는 작게 나타났다. 연구지역 이산화탄소 플럭스의 계절적인 변화특성은 봄의 경우 식생과 비식생 지역 모두 호흡에 의한 방출이 우세하였으며, 여름은 식생 지역에서 방출이 우세하였고 비식생 지역은 식물플랑크톤의 광합성에 의한 흡수가 확인되었으나 매우 미미하였다. 겨울은 식생 지역에서 이산화탄소 플럭스의 변화가 거의 나타나지 않았으며, 비식생 지역은 흡수가 일부 확인되었으나 매우 미미하였다.

동태평양 열대해역에서 2009-2010년 침강입자 플럭스의 수직 변화 (Vertical Variation of the Particle Flux in the Eastern Tropical Pacific from 2009 to 2010)

  • 김형직;조소설;김동선;김경홍;유찬민
    • Ocean and Polar Research
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    • 제44권3호
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    • pp.221-233
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    • 2022
  • A sediment trap had been deployed at 1250 m depth in the Eastern Tropical Pacific (ETP) from September 2009 to July 2010, with the aim of understanding the temporal and vertical variability of particle flux. During the monitoring period, total particle flux varied from 12.4 to 101.0 mg m-2day-1, with the higher fluxes in January-March 2010. Biogenic particle flux varied in phase with the total particle flux. The increase in total particle flux during January-March 2010 was attributed to the enhanced biological production in the surface layer caused by wind-driven mixing in response to the seasonal shifts in the location of the Intertropical convergence zone. The export ratio (e-ratio) was estimated using the particulate organic carbon flux and satellite-derived net primary production data. The estimated e-ratios changed between 0.8% and 2.8% (1.4±0.6% on average). The ratio recorded in the negative phase of Pacific decadal oscillation (PDO) was similar to the previous results obtained from the ETP during the 1992/93 periods in the positive phase of PDO. This suggests that the regime shift of the PDO is not related to the carbon export ratio.

에디 공분산 기반의 플럭스 타워 관측자료를 이용한 두만강 유역 습지 생태계 CO2 흡수량 분석 (Estimating carbon dioxide uptake in wetland ecosystems of Tumen River Basin using eddy covariance flux data)

  • ;;;이동근
    • 한국환경복원기술학회지
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    • 제27권3호
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    • pp.67-74
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    • 2024
  • In the context of rapid temperature rise in mid-to-high latitude regions, cold region wetlands have become a hotspot for current wetland carbon cycle research due to their high sensitivity to climate change. Strengthening the monitoring of CO2 fluxes in wetland ecosystems is of great practical significance for clarifying the carbon balance of wetlands and maintaining the ecological balance of wetland ecosystems in China's high latitude regions. In this study, the carbon flux (NEE, Net ecosystem exchange; GPP, Gross Primary Production; RECO, Ecosystem response) of Jingxin Wetland was monitored by eddy correlation method from August 2021 to March 2024.2022-2023 shows CO2 sinks, absorbing 349.4 g C·m-2·yr-1 annually. The correlation analysis showed that Ta, VPD and PPFD were the main environmental factors affecting CO2 flux in Jingxin wetland.

한국 연안 양식패류 패각 재활용을 통한 탄소수지 추정 (리뷰) (Estimation of Carbon Flux caused by the shell re-treatment at coastal shellfish aquaculture fields in Korea (Review))

  • 박영철;유재원;최근형;이창근;김혜정
    • 한국습지학회지
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    • 제25권1호
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    • pp.1-13
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    • 2023
  • 연안의 양식 어장에서 식량자원으로 주로 생산되는 양식 패류는 성장에 따라 탄소가 저장된 패각을 형성한다. 수확된 패류를 식량자원으로 사용하고 남겨지게 되는 패각 부분을 탄소저장기능이 있는 방법으로 재활용할 경우, 탄소수지 개선 효과가 있다는 것이 여러 연구를 통해 보고되었다. 본 연구에서는 해양 패류의 개체성장에 따른 패각 내 이산화탄소의 생성과 저장에 대한 기작을 분석하였고 자연 패류 및 양식 패류의 생산성 향상을 통하여 일반적으로 얻어지는 탄소수지 추정에 대하여 고찰하였다. 또한 양식 패류 생산의 부산물로 얻어지는 패각의 재활용방안에 대하여 분석하였으며 마지막으로 탄소저장 기능성을 가진 재활용 방법이 활용될 경우에 대한 탄소수지를 추정함으로써 온실가스 감축에 대한 잠재효과를 고찰하였다.