• Korean Journal of Environmental Biology
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• v.18 no.3
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• pp.315-322
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• 2000

Effects of the simulated acid rain on the growth response and the structural features were studied with the 3 month old seedlings of G. biloba and P. thunbergii treated with acid rain of pH 5.6, 4.0, 3.2, and 2.4. The diameter and area of tracheid cells in the transectioned stem of G. biloba decreased with acidity of simulated acid rain. The wall thickness of tracheid cells was the thinest at pH 2.4, but there was no different at other levels of pH. Increasing of the acidity, the height of tracheid cells were reduced steadily. The diameter and area of tracheid cells of the transectioned root reduced with decreasing pH of acid rain, but those at pH 3.2 were larger than those at control. The wall thickness and height of tracheid cells of root were gradually decreased with acidity of acid rain. The size variation of the fusiform cambial initials in the stem of G. biloba sections tangentially showed a shortening tendency with treatment of acid rain. The length of ray initials was the shortest at pH 2.4 and reduced with decreasing pH of acid rain. The diameter, area, wall thickness, and height of the tracheid cells in P. thunbergii stem and root decreased with decreasing pH of acid rain. The areas of the pith, cortex, and xylem in P. thunbergii treated with acid rain decreased, but the cortex and pith areas increased significantly after exposure to acid rain of pH 3.2 compared with control.

• BMB Reports
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• v.49 no.3
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• pp.149-158
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• 2016

Plants have evolved a vast chemical cornucopia to support their sessile lifestyles. Man has exploited this natural resource since Neolithic times and currently plant-derived chemicals are exploited for a myriad of applications. However, plant sources of most high-value natural products (NPs) are not domesticated and therefore their production cannot be undertaken on an agricultural scale. Further, these plant species are often slow growing, their populations limiting, the concentration of the target molecule highly variable and routinely present at extremely low concentrations. Plant cell and organ culture constitutes a sustainable, controllable and environmentally friendly tool for the industrial production of plant NPs. Further, advances in cell line selection, biotransformation, product secretion, cell permeabilisation, extraction and scale-up, among others, are driving increases in plant NP yields. However, there remain significant obstacles to the commercial synthesis of high-value chemicals from these sources. The relatively recent isolation, culturing and characterisation of cambial meristematic cells (CMCs), provides an emerging platform to circumvent many of these potential difficulties.

• Journal of Plant Biology
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• v.34 no.4
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• pp.311-316
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• 1991

The origin of cambial initials from procambium was studied in the seedling root of Acer saccharinum. In transverse view, the first periclinal divisions of procambial cells occurred just outside of each early metaxylem and resulted in meristematic strips. As root development progressed, the division activities appeared subsequently outside of each late metaxylem and then in pericycle cells opposite the four protoxylem poles. Eventually, such meristematic strips were connected completely each other. Thus, a nearly rectangular shaped meristematic layer in outline was formed outside the xylem in a whole root transectioned. In tangential section, early procambium showed a homogeneous structure consisted of uniform short cells with transverse end walls. However, some of the procambial cells did elongate, whereas others divided transversely. The former become more elongate, tapered, and vacuolated. Finally, they differentiated into fusiform initials. Short cells consisting axial strands divided continuousely in transverse plane and became ray initials, while some short cells elongated and transformed into long cells. The early ontogeny of vascular cambium in Acer saccharinum root was interpreted to be established by a gradual process.

• Journal of Plant Biology
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• v.32 no.4
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• pp.305-312
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• 1989

Developmental anatomy was conducted in order to elucidate the differentiating pattern of fascicular cambial initials in the hypocotyl of Ricinus communis. The homogeneous procambium with relatively short cells in early stage is transformed into a heterogeneous structure with long and short cells in late stage in tangential view. Fusiform and ray initials are gradually originated from the long and short cells of the procambium in hypocotyl in later stage respectively. Fusiform initials are not shorter than procambial cells because of the successive elongation of vascular meristematic cells. Therefore, the distinction between procambium and fascicular cambium is not made from comparison with their cell length. The characteristics of the fascicular cambium are gradully acquired at or just after completion of hypocotyl elongation.

• Applied Microscopy
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• v.2 no.1
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• pp.33-37
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• 1972

The distribution, ultrastructure, and cytochemical properties of microbodies (peroxisomes) from the main roots and mature leaves of Panax ginseng were studied by electron microscopy included the activity of catalase in 3, 3'-diaminobenzidine (DAB) medium at pH 9, using glutaraldehyde-fixed tissues. The microbodies, which are about $0.5{\sim}1.5{\mu}$ diameter, were described from mesophyll cells of mature leaves and storage cells and cambial cells of main roots. The microbodies of the ginseng include the coreless, homogeneous matrix, in which catalase activities are present.

• Journal of the Korean Wood Science and Technology
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• v.36 no.4
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• pp.84-92
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• 2008

Anatomical and physical characteristics of Pinus densiflora woods damaged by forest fire at Yangyang-gun, Kangwon-do were investigated in present study. Even though the bark was severly carbonized, no trace of carbonization in the xylem was found. The amount of resin exudation was different by the degree of fire damage. Green moisture content of sapwood in the damaged wood was lower than that in the sound wood, but vice versa in the heartwood. Green density of heartwood in the damaged wood was higher than that in the sound wood, but there was no significant difference in sapwood. Cambial activities were found stopped in the severely damaged wood but were identified in the lightly damaged wood. In safranine staining process, epithelial and ray parenchyma cell walls in the damaged woods showed darker hue than those in the sound wood. Granular substances were observed in the lumina of ray parenchyma and epithelial cells of the severely damaged wood. Relative crystallinity of the outermost growth ring in the severely damaged wood was slightly higher than that in the sound wood.

• Journal of Plant Biology
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• v.38 no.1
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• pp.115-119
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• 1995

In order to clarify the relationship between the activity and age of vacular cambium in the rhizome of Botrychium ternatum, the cambia of the 6th and 10th internodes from its shoot apex were compared ultrastructurally and histochemically. The cambium in the 6th internode had two to four radially arranged cells in each row, and the cells are radially flattened and had thiner tangential walls than radial. The cambial cells were ultrastructurally characterlized by the presence of a large vacuole, few storage materials such as starch grains and lipid droplets, and various cell organelles such as mitochondria, endoplasmic reticulums and dictyosomes in cytoplasm. On the other hand, the cells of cambium in the 10th internode showed irregular arangement and somewhat enlargement in radial plane. The cells were filled with numerous storage materials and small vacuoles and had few cell organelles except for mitochondria. In addition, the activity of acid phosphatase appeared in the cambium of the 6th internode but of the 10th. From the above results, it is clear that the vascular cambium of the 6th internode was active and that of the 10th was dormant. Thus we reached a conclusion that the rhizome of '||'&'||'trychium ternatum has determinate cambium.

• Journal of Plant Biotechnology
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• v.49 no.3
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• pp.171-177
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• 2022

The precise homeostatic regulation of local auxin accumulation in xylem precursors of cambium stem cell tissues is one of the most important mechanisms for plant vascular patterning and radial secondary growth. Walls are thin (WAT1), a novel intracellular auxin transporter, contributes directly to the auxin accumulation maxima in xylem precursors. According to recent research, the auxin signaling activated pathway-related gene network was significantly enriched during the secondary growth of Panax ginseng storage roots. These imply that during P. ginseng root secondary growth, specific signaling mechanisms for local auxin maxima in the vascular cambial cells are probably triggered. This study identified four WAT1-like genes, PgWAT1-1/-2 and PgWAT2-1/-2, in the P. ginseng genome. Their expression levels were greatly increased in nitratetreated storage roots stimulated for secondary root growth. PgWAT1-1 and PgWAT2-1 were similar to WAT1 from Arabidopsis and tomato plants in terms of their subcellular localization at a tonoplast and predicted transmembrane topology. We discovered that overexpression of PgWAT1-1 and PgWAT2-1 was sufficient to compensate for the secondary growth defects observed in slwat1-copi loss of function tomato mutants. This critical information from the PgWAT1-1 and PgWAT2-1 genes can potentially be used in future P. ginseng genetic engineering and breeding for increased crop yield.

• Journal of Plant Biotechnology
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• v.46 no.3
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• pp.180-188
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• 2019

Auxin plays a crucial regulatory role in plant growth and development processes. Three major classes of auxin-responsive transcription factors controlled by the Auxin/indole-3-acetic acid (Aux/IAA), Gretchen Hagen 3 (GH3), and small auxin up RNA (SAUR) genes regulate auxin signaling. Aux/IAA, in particular, encodes short-lived nuclear proteins that accumulate rapidly in response to auxin signaling. In this study, we isolated a PagAux/IAA1 gene from poplar (Populus alba ${\times}$ P. glandulosa) and investigated its expression characteristics. The PagAux/IAA1 cDNA codes for putative 200 amino acids polypeptide containing four conserved domains and two nuclear localization signals (NLSs). Utilizing Southern blot analysis, we confirmed that a single copy of the PagAux/IAA1 gene was present in the poplar genome. The expression of this gene is specific to leaves and flowers of the poplar. PagAux/IAA1 expressed in the early exponential growth phase of cell-cultured in suspension. PagAux/IAA1 expression level reduced in drought and salt stress conditions, and the presence of plant hormones such as abscisic acid. However, expression enhanced in cold stress, cambial cell division, and presence of plant hormones such as gibberellic acid and jasmonic acid. Thus, these results suggest that PagAux/IAA1 participates in cold stress response as well as developmental processes in the poplar.

• The korean journal of orthodontics
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• v.10 no.1
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• pp.7-13
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• 1980

To study some informations on the morphogenesis and developmental process of the coronal suture in rats, the author performed daily oral administrations of demethylchlorotetracycline, a kind of tetracycline group, in the amount of 30mg/kg of body weight to the female rats from the 7th day of pregnancy to the time of delivery. Microscopic evaluation was undertaken on the fetal rats in the experimental group. The subject of this experiment were defined to the fetal rats of each group at 1st, 3rd, 7th and 14th day after birth. All these fetal rats were sacrificed and the heads were removed. All the tissue sections were fixed with $10\%$ formalin, Bouin' and Carnoy' solution and then stained by Hematoxylin-Van Gieson stain, or Feulgen and Rossenbeck, Periodic acid Schiff, and prepared for alcian blue reaction. The results were as follows; 1. The directions of osteogenic fibers were arranged irregulary during first 3 days, but after the 7th day they tended to change radial directions like control group. 2. The density of deep stained cells by Feulgen-Rossenbeck reaction were shown leu in the experimental group than that in the control group in first 3 days, but there was shown no significant difference between both groups after the 7th day. 3. PAS reaction in early stage was generally negative in the experimental group unlike as in the control group, but diffuse reaction was observed in the loose middle zone like as in the control group after 14th day. 4. Alcian blue reaction was negative in cambial zone, and slightly positive in uniting zone compared with control group in early stage. After 14th day, however, there was observed a tendency of moderately positive reaction.