• Title/Summary/Keyword: calcium ions

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Production of Peptides Enhancing Calcium Solubility in the Presence of Phosphate Ions In Vitro (In Vitro 상에서인 이온 존재 하에서의 칼슘 용해도를 증대시키는 펩타이드의 생산)

  • 이윤동;이현수
    • The Korean Journal of Food And Nutrition
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    • v.10 no.4
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    • pp.485-490
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    • 1997
  • Gluten peptide was produced from corn gluten by enzymatic hydrolysis. This peptide had an ability to increased the solubility of calcium owing to protect calcium ions from forming precipitates of calcium phosphate in the presence of phosphate ions. The solubility of calcium was increased 5.2 times in the presence of 8.3 mg peptide produced by the treatment of papain. These peptides contained high acidic amino acids and fractionated by Delta pack column into fractions No. 1, No. 2 and No. 3. Among them the fraction No. 3 had the highest calcium binding capacity.

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Effects of calcium and calcium antagonist nifedipine on the glycogenolysis induced by the stimulation of alpha-and beta-adrenergic receptors in rat hepatocytes (흰쥐 hepatocyte에서 알파 및 베타 아드레날린 수용체의 자극에 의한 글리코겐분해에 있어서 칼슘과 니페디핀의 작용)

  • Lee, Young-Hee;Kim, Joon-Kyum;Kim, Mie-Young
    • YAKHAK HOEJI
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    • v.32 no.6
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    • pp.428-434
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    • 1988
  • The effects of calcium and calcium antagonist, nifedipine on the adrenergic receptor-stimulated glycogenolysis were investigated in isolated rat hepatocytes. The hepatic glycogenolysis induced by alpha-adrenergic receptor stimulation depended on calcium ions, and beta-adrenergic activation was unrelated to calcium ions. Nifedipine decreased the alpha-adrenergic agonist-induced glucose release significantly and the decrease was depended on calcium ions. The glucose release induced by beta-adrenergic agonist was not inhibited by nifedipine.

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Fluoride and Calcium in Tea Leaves

  • Takiyama, Kazuyoshi;Ishii, Yuuko
    • Analytical Science and Technology
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    • v.8 no.4
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    • pp.877-880
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    • 1995
  • The determination of fluoride and the nutritious calcium in infusion of teas are explained. Tea leaves were pulverized and were immersed in boiling water. The solution was filtered and fluoride, calcium and oxalic acid were determined by the ion chromatography. The quantities of fluoride, calcium and oxalate ions extracted from 100 g of tea leaves were calculated. Tea leaves were also immersed in 0.5 M hydrochloric acid and extracted oxalate and calcium ions were analyzed. The free oxalic acid and calcium were extracted in boiling water and the total ones were extracted in hydrochloric acid. The quantity of calcium oxalate was calculated from the total and the free oxalic acids. The free calcium was estimated to be nutritious.

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Influence of Pulsed Electric Field on Accumulation of Calcium in Lactobacillus rhamnosus B 442

  • Goral, Malgorzata;Pankiewicz, Urszula;Sujka, Monika;Kowalski, Radoslaw;Giral, Dariusz;Kozlowicz, Katarzyna
    • Journal of Microbiology and Biotechnology
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    • v.30 no.1
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    • pp.44-53
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    • 2020
  • Calcium is an element that performs many important functions in the human body. A study was conducted on the use of a pulsed electric field (PEF) to enrich cells of Lactobacillus rhamnosus B 442 in calcium ions. The highest concentration of calcium ions in bacterial cells (7.30 mg/g d.m.) was obtained at ion concentration of 200 ㎍/ml of medium and with the use of the following PEF parameters: field strength 3.0 kV/cm, exposure time 10 min, pulse width 75 ms and 20 h of culturing after which bacteria were treated with the field. Cell biomass varied in the range from 0.09 g/g d.m. to 0.252 g/g d.m., and the total number of bacteria ranged from 1010 CFU/ml to 1012 CFU/ml. Microscope photographs prove that calcium ions were situated within the cells of the bacteria, and electroporation contributed to an increase in the effectiveness of the ion bioaccumulation process. Samples containing calcium and subjected to electroporation displayed intensive fluorescence. The significance of this research was the possibility of using probiotic bacteria enriched with calcium ions for the production of functional food in subsequent studies.

HREM Analysis of Apatite Formation in Modified-Simulated Body Fluid Containing Bovine Serum Albumin (소 혈청 알부민이 함유된 유사체액 내에서 아파타이트의 생성에 대한 고분해능 전자현미경 분석)

  • Kim, Woo Jeong;Lee, Kap Ho;Hong, Sun Ig
    • Korean Journal of Metals and Materials
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    • v.46 no.2
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    • pp.105-110
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    • 2008
  • Process of the hydroxyapatite (HA) formation on bioactive titanium metal prepared by NaOH treatment in a modified-simulated body fluid (mSBF) containing bovine serum albumin (BSA) was investigated by high resolution transmission electron microscope attached with energy dispersive X-ray spectrometer (EDX). The amorphous titanate, which was formed on titanium surface by NaOH treatment, combined with the calcium ions in the liquid to form an amorphous calcium titanite. With increasing of soaking time in the liquid, an amorphous calcium titanite combined with the phosphate ions to form an amorphous calcium phosphate with low Ca/P atomic ratio, and it grows as aggregates of plate (or needle)-like substance on titanium surface. The crystalline apatite layers, which are needle-shaped with the c axis parallel to the long axis, are formed in an amorphous calcium phosphate with further increase in soaking time. The formation of needle-shaped apatite layers can be explained by electrostatic effects and difference of concentration between calcium, phosphate, and albumin ions.

A Study on the Characteristics of Calcium Ion Separation by Adding Acidic/Basic Reagents (산/염기성 물질 주입에 따른 칼슘이온 분리 특성 연구)

  • Lee, Ye Hwan;Kim, Jeongeun;Kim, Sung Su
    • Applied Chemistry for Engineering
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    • v.31 no.1
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    • pp.103-107
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    • 2020
  • The purpose of this study is to resource calcium ions contained in most industrial by-products, and confirm the characteristics of calcium ions by extraction and separation conditions. Calcium oxide was used as a calcium extraction source, and hydrochloric acid as an extraction solvent, and the extraction amount according to the concentration of the extraction solvent and the pH dependent characteristics of the extract were analyzed. As the extractant concentration increased, the extracted amount increased while the pH for the extraction was kept constant. In order to separate extracted calcium ions, an acid-basic solution was injected and the formation of precipitates and also the form of precipitates were analyzed. When the sulfuric acid and sodium hydroxide solution of acid and basic substances were injected into the calcium extract, precipitates were formed and separated into CaSO4 and Ca(OH)2 forms.

A Study on the Biological Treatment of Acid Pickling Wastewater Containing a High Concentration of Nitrate Nitrogen (고농도 질산성 질소를 함유한 산세폐수의 생물학적 처리에 관한 연구)

  • Park, Sang Jin;Lee, Sang Houck
    • Journal of Korean Society on Water Environment
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    • v.31 no.3
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    • pp.253-261
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    • 2015
  • The purpose of this study is the efficient biological treatment of highly concentrated nitrate nitrogen by calcium ion control present within the pickling wastewater. In laboratory scale's experiments research was performed using a sequencing batch reactor and the evaluation of denitrification reaction in accordance with the injection condition of calcium ions, the surface properties of microorganisms, and the evaluation of sludge precipitability were performed. Results of the study showed that the denitrification reaction was delayed when injecting more than 600 mg/L of the calcium ion within the denitrification process. In addition, we observed the absorption form of calcium ions absorbed on the surface of microorganisms following an increase in the calcium ion dose. It was found that as the calcium ion dose increased the sludge precipitability also increased continuously and it is judged that a smooth denitrification induction is possible when treating the nitrate nitrogen by the calcium ion control of pickling waste water and the shortening of precipitation time enables a liquid operation to increase the reaction time.

A STUDY OF IONIC DISSOCIATION ON VARIOUS CALCIUM HYDROXIDE PASTES USING MOLECULAR SIEVING MODEL (분자체 모델을 이용한 수종의 수산화칼슘 제재의 이온 용출에 관한 연구)

  • Lee, Kyoung-Sun;;;Lee, Seung-Jong
    • Restorative Dentistry and Endodontics
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    • v.27 no.6
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    • pp.632-643
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    • 2002
  • The purpose of this study was two-fold. First was to evaluate whether the molecular sieving model was appropriate for ionic dissociation experiment. Second was to compare the dissociation of calcium and hydroxyl ions from five types of calcium hydroxide pastes (Pure calcium hydroxide paste, DT temporary $dressing^{\circledR},{\;}Metapaste^{\circledR},{\;}Chidopex^{\circledR},{\;}Metapex^{\circledR}$) in three vehicles (aqueous, viscous and oily) and the antibacterial effect. Each calcium hydroxide pastes was placed into 0.65ml tube with cap and then 15% polyacrylamide gel was placed onto calcium hydroxide pastes. After the gel was hardened, the tubes were filled with tridistilled water (pH 7.14) and closed with cap. The tubes were stored in $37^{\circ}C$ 100% incubator The pH reading and the concentration of calcium ions were taken at 1, 4, 7. 10, and 14 days. The brain heart infusion agar plates with S. mutans and A. actinomycetemcomitans were used far antibacterial activity test. Middle of agar plate was filled with the calcium hydroxide pastes. The plates were incubated at $37^{\circ}C$ and observations were made to detect the zones of inhibition. These data were evaluated statistically by use of the analysis of variance and duncan test. The results were as follows. 1. In fresh mixing state, the pH of five types of calcium hydroxide pastes were measured between 12.5 and 12.8. 2. The pH was increased in all five types of calcium hydroxide pastes compared with control group. In 14 days, Pure calcium hydroxide paste (11.45) and DT temporary $dressing^{\circledR}$ (11.33) showed highest pH, followed by $Metapaste^{\circledR}$ (9.49), $Chidopex^{\circledR}$ (8.37) and $Metapex^{\circledR}$ (7.59) 3. Calcium was higher in all five types of calcium hydroxide pastes compared with control group. In 14 days, Pure calcium hydroxide paste (137.29 mg%) and DT temporary $dressing^{\circledR}$ (124.6 mg%) showed highest value, followed by $Metapaste^{\circledR}$ (116.74 mg%), $Chidopex^{\circledR}$ (111.84 mg%) and $Metapex^{\circledR}$ (60.22 mg%). 4. The zones of bacterial inhibition were seen around all five types of calcium hydroxide pastes. $Chidopex^{\circledR}{\;}and{\;}Metapex^{\circledR}$ groups which include iodoform were observed significantly larger zone of inhibition in A. actinomycetemcomitans compared with the other calcium hydroxide groups (p<0.05) However, $Metapex^{\circledR}$ showed the least antibacterial effect on S. mutans compared with other groups (p<0.05). The molecular sieving model was found to be acceptable in dissociation experiment of hydroxyl and calcium ions when compared with the previous tooth model study. But this model was not appropriate for the antibacterial test.

Structural basis of Ca2+ uptake by mitochondrial calcium uniporter in mitochondria: a brief review

  • Jiho, Yoo
    • BMB Reports
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    • v.55 no.11
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    • pp.528-534
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    • 2022
  • Mitochondria are cellular organelles that perform various functions within cells. They are responsible for ATP production, cell-signal regulation, autophagy, and cell apoptosis. Because the mitochondrial proteins that perform these functions need Ca2+ ions for their activity, mitochondria have ion channels to selectively uptake Ca2+ ions from the cytoplasm. The ion channel known to play the most important role in the Ca2+ uptake in mitochondria is the mitochondrial calcium uniporter (MCU) holo-complex located in the inner mitochondrial membrane (IMM). This ion channel complex exists in the form of a complex consisting of the pore-forming protein through which the Ca2+ ions are transported into the mitochondrial matrix, and the auxiliary protein involved in regulating the activity of the Ca2+ uptake by the MCU holo-complex. Studies of this MCU holo-complex have long been conducted, but we didn't know in detail how mitochondria uptake Ca2+ ions through this ion channel complex or how the activity of this ion channel complex is regulated. Recently, the protein structure of the MCU holo-complex was identified, enabling the mechanism of Ca2+ uptake and its regulation by the MCU holo-complex to be confirmed. In this review, I will introduce the mechanism of action of the MCU holo-complex at the molecular level based on the Cryo-EM structure of the MCU holo-complex to help understand how mitochondria uptake the necessary Ca2+ ions through the MCU holo-complex and how these Ca2+ uptake mechanisms are regulated.

Solidification and Leaching Characteristics of Cyclone Ash from Industrial Incineration Plant

  • Lee, Dong-Choon;Kim, Young-Ju
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.10 no.S_2
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    • pp.89-95
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    • 2001
  • The solidification and leaching characteristics of cyclone ash collected from an industrial incineration plant were investigated. Cement and calcium hydroxide were used as the solidifying materials. The leaching characteristics of the solidified cyclone ash were found to vary depending on both the quantitative and the qualitative aspects of the solidifying materials. Except for copper and lead, all the heavy metal ions in the leachate of the solidified material composed of 10~20 % cement or 10~20 % calcium hydroxide were found to be within their standard limit. Moreover, all the heavy metal ions were also observed to be within satisfactory limits in the leachate obtained from the solidified material composed of 30 % cement or 30 % calcium hydroxide. Therefore, to satisfy the standard compressive intensity and permissible limits of heavy metal ions leached from solidified material, it would appear that a 30 % proportion of either additive in the solidification product can meet the required standard for the leachate. The cost of solidifying cyclone ash per ton for ash-30 % cement and ash-30 % lime was calculated as 26,750 and 26,070 won, respectively. Accordingly, significant reduction in the waste toxicity and mobility as well as an improvement in the engineering properties of the solidified products were successfully achieved.

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