Korean Journal of Metals and Materials (대한금속재료학회지)

The Korean Institute of Metals and Materials (대한금속재료학회)
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HREM Analysis of Apatite Formation in Modified-Simulated Body Fluid Containing Bovine Serum Albumin

소 혈청 알부민이 함유된 유사체액 내에서 아파타이트의 생성에 대한 고분해능 전자현미경 분석

  • Kim, Woo Jeong (Division of Nano Technology, Chungnam National University) ;
  • Lee, Kap Ho (Division of Nano Technology, Chungnam National University) ;
  • Hong, Sun Ig (Division of Nano Technology, Chungnam National University)
  • 김우정 (충남대학교 공과대학 나노공학부) ;
  • 이갑호 (충남대학교 공과대학 나노공학부) ;
  • 홍순익 (충남대학교 공과대학 나노공학부)
  • Received : 2007.11.16
  • Published : 2008.02.10
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Abstract

Process of the hydroxyapatite (HA) formation on bioactive titanium metal prepared by NaOH treatment in a modified-simulated body fluid (mSBF) containing bovine serum albumin (BSA) was investigated by high resolution transmission electron microscope attached with energy dispersive X-ray spectrometer (EDX). The amorphous titanate, which was formed on titanium surface by NaOH treatment, combined with the calcium ions in the liquid to form an amorphous calcium titanite. With increasing of soaking time in the liquid, an amorphous calcium titanite combined with the phosphate ions to form an amorphous calcium phosphate with low Ca/P atomic ratio, and it grows as aggregates of plate (or needle)-like substance on titanium surface. The crystalline apatite layers, which are needle-shaped with the c axis parallel to the long axis, are formed in an amorphous calcium phosphate with further increase in soaking time. The formation of needle-shaped apatite layers can be explained by electrostatic effects and difference of concentration between calcium, phosphate, and albumin ions.

Keywords

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