• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.37-37
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• 2019

Cryopreservation is one of the ideal and suitable methods for long-term storage of plant germplasm. The plant contaminated with diseases and pathogens are decreased the multiplication rate, survival rate and high quality of plants after cryopreservation. The aim of this work was to improve a micropropagation method for lily in Korea, which are cultivars and indigenous plant. In the last process of rinsing scales after surface-sterilization, we tried to control the diseases and pathogens lived within the tissue by rinsing in 0.03% sodium hypochlorite (NaClO) instead of sterile distilled water. Bulb scales of Lilium were cultured in vitro on MS medium supplemented with Plant Preservative Mixture (PPM). The newly small bulb formed from bulb-scales was transferred to MS medium. We checked the non-contamination and survival rate after 2 weeks in culture. Non-contamination was shown to be 70 to 90% in formed small bulbs. This study will help to mitigate microbial contamination in Lilium species micropropagation for cryopreservation.

• 식물조직배양학회지
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• 제26권2호
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• pp.103-107
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• 1999

Lilium oriental hybrid 'Casa Blanca'의 기내인편에서 자구를 대량생산하기 위하여 MS배지의 염류농도 및 당, 광조건 등이 자구의 형성 및 비대에 미치는 영향을 조사하였다. MS배지의 1/2～2배 염류농도에서 자구의 형성은 차이를 나타내지 않았으나 3배 염류농도에서 자구의 형성이 현저히 억제되었다 자구의 비대는 MS기본배지에서 가장 촉진되었다. 배지의 NH$_4$$^{+}$ : NO$_3$의 비율이 1 : 2와 1 : 3일 때, 또는 MS기본배지 (NH$_4$$^{+}$ : NO$_3$= 약 1 : 2)가 인편에서 자구의 형성 및 비대에 적절하였다. 따라서 MS 기본배지가 자구의 형성 및 비대에 양호한 것으로 나타났다. 당의 농도가 높아지면 자구형성은 억제되고 자구비대는 촉진되었으며, 당 9~12% 첨가배지에서 자구비대가 양호하였다. 배지내 활성탄의 첨가는 인편에서 자구의 형성을 억제하였으나 형성된 자구의 비대는 촉진하였다. 특히 활성탄 1 g/L를 첨가한 배지가 자구의 비대에 가장 효과적이었다. 자구의 형성 및 형성된 자구의 비대는 명, 암간에 차이를 나타내지 않았다. 따라서 L. Oriental Hybrid 'Casa Blanca'의 기내 대량번식은 당 9%와 활성탄 1 g/L가 첨가된 MS기본배지에서 기내인편을 배양하여 자구를 형성 및 비대시키고 형성된 자구의 인편을 계대배양하는 것이 효율적이었다.

• 한국식품저장유통학회지
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• 제16권1호
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• pp.82-86
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• 2009

양파 구의 부위 및 크기에 따른 pyruvic acid 함량과 양파 식물체의 구, 엽초, 엽 및 엽서에 따른 부위별 pyruvic acid와 당 함량을 분석하여 변화양상을 관찰하였다. 그 결과 양파구의 하부에서 pyruvic acid 함량이 가장 높았고, 다음이 상부였으며 중간부분의 함량이 가장 낮았다. 인편에 따라서는 외피로부터 2번째 인편에서 가장 낮았으며 3번째 인편부터는 구의 내부로 갈수록 pyruvic acid 함량이 증가하였다. 생육기간 경과에 따른 구, 엽초부 및 엽의 pyruvic acid 함량은 구비대 초기에는 구에 비해 엽과 엽초부의 pyruvic acid 함량이 더 높았으나 수확기에 이르러서는 구의 pyruvic acid 함량은 다소 증가하였지만 엽과 엽초부는 큰 폭으로 감소하였다. 총당 함량은 구비대 초기에는 큰 차이가 없었으나 구가 비대되면서 구의 총당 함량은 큰 폭으로 증가하였고, 엽과 엽초부는 감소하였다. 그리고 엽서에 따라서는 바깥쪽의 오래된 잎에서 pyruvic acid와 총당 함량이 가장 낮았으며 내부의 어린잎으로 갈수록 높아지는 경향이었다.

• The Plant Pathology Journal
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• 제19권1호
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• pp.43-45
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• 2003

Soft rot symptom was observed on lily bulb in the fields and at a low temperature storage house from 1999 to 2000 in Korea. The small dark-brown lesion appeared on the bulb, and enlarged and developed into the inner scales of the bulb. The bulb became water soaked and gave out unpleasant odor. Two different pathogenic bacteria were isolated from infected tissues. The causal bacteria were identified as Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora) and Pseudomonas marginalis based on bacteriological characteristics. Pathogenicity of the bacteria was proven by Koch's postulations. This is the first report of bacterial soft rot of lily bulb in Korea caused by the two bacteria.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.43-43
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• 2020

Plants regenerated from in vitro cultures are associated with chromosomal variations, which have been generally found in long-term culture. Reducing plant culture age is one of the ways to reduce genetic and epigenetic changes. The present study focused on the efficient in vitro propagation of lily cultivars and has intensified to speed up bulb propagation for cryopreservation. The multiplication process applied in this experiment uses starting material, which the newly small bulb formed from bulb-scales in two lily cultivars. The adventitious bulb from bulb-scale tissue cultured on three different media following Murashige and Skoog (MS) basal medium supplemented with 1 g/L Charcoal, MS medium containing 0.3 mg/L IAA and 0.4 mg/L BA hormone with or without Charcoal, respectively. After about seven weeks, there is little change in the number of newly propagated bulbs in small bulbs of the two media. Compared to the both mediums, the number of the propagated bulbs is increased 5 times in MS medium containing 0.3 mg/L IAA and 0.4 mg/L BA hormone without Charcoal. After about seven weeks, the results of the propagation showed that the number of the propagated bulbs is increased 5 times in MS medium containing 0.3 mg/L IAA and 0.4 mg/L BA hormone without Charcoal compared to the both mediums. The number of propagated bulbs ranged from 5 to 6 and 4 to 6 with an average of 5 in Tropicalpink and Greenstar cultivars, respectively. There is little change in the number of newly propagated bulbs in small bulbs of other media. The multiplication process applied in this study may save in vitro culture period and effort.

• 한국자원식물학회지
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• 제19권3호
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• pp.432-435
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• 2006

A rapid and mass propagation method for multiple shoots and plant regeneration using bulb scales of Muscari comosum var. plumosum were developed. In vitro different parts of bulb scale as explants were cultured on 11 kinds of MS (1962) media supplemented with various plant growth regulators to induce shoot and callus. A combination of 2.0 mg/L 6-BA and 2.0 mg/L IBA on MS medium was the most favorable and induced the highest production (80%) of shoot formation after 30 days. We also found that the middle part of bulb scale was the best for mass propagation of Muscari comosum var. plumosum of which production could reach 64.4%.

• 한국자원식물학회지
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• 제32권6호
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• pp.730-734
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• 2019

Cryopreservation is one of the ideal and suitable methods for long-term storage of plant germplasm. The plant contaminated with diseases and pathogens are decreased the multiplication rate, survival rate and high quality of plants after cryopreservation. The aim of this work was to improve a micropropagation method for lily in Korea, which is indigenous plant. In the last process of rinsing scales after surface-sterilization, we tried to control the diseases and pathogens lived within the tissue by rinsing in 0.03% sodium hypochlorite (NaClO) instead of sterile distilled water. Bulb scales of Lilium were cultured in vitro on MS medium supplemented with Plant Preservative Mixture (PPM). The results showed that L. tsingtauense accessions were observed ranged from 53.9 to 100% with a mean value of 76.8% and L. hansonii accessions were checked from 84.5 to 85.5% with a mean of 85% survival rate. The newly small bulb formed from bulb-scales was transferred to MS medium. We checked the presence of microorganisms and survival rate after 3 weeks in culture after examination of bacterial incidences. The results indicated that the non-contamination rate were shown ranged from 75.0 to 94.1% with mean value of 83.2% in L. tsingtauense species, and that L. hansonii were observed 85.1 to 91.7% with mean value of 88.4%. This study will provide a valuable basis for establishment of effective axenic cultures for in vitro micropropagation of Korean native lily species.

• 한국자원식물학회지
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• 제36권3호
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• pp.237-255
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• 2023

Variations in phenotypic traits are important for onion genetic improvement. The aim of this study was to identify the phenotypic traits of temporary genetic resources and the best accessions for the development of onion breeding programs. Sixteen phenotypic traits of 79 onion accessions were studied. The descriptive statistics of phenotypic traits exhibited a high variation in onion accessions. Among the 79 evaluated accessions, 64.55% had a large bulb neck width and 44.30% had a circular bulb shape. Principal component analysis showed that six principal components (PCs) accounted for 72.65% of the total variation. The main factors contributing to PC1 were bulb weight, equatorial and bulb polar diameters, plant height, and degree of splitting into bulblets, whereas those contributing to PC2 were the bulb color of the epidermal cells of the fleshy scales and color of the dry skin on the bulb. The accessions were classified into three groups-clusters 1, 2, and 3. Cluster 2 accessions were the most suitable for selecting large and circular bulb-shaped onion cultivars. The phenotypic variation observed in this study may help to select potential accessions for breeding new onion cultivars.

• 식물조직배양학회지
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• 제22권2호
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• pp.89-93
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• 1995

기내자구 소인편조직을 배양할 때, sucrose 3%, 2,4-D 0.05 mg/L이하에서, 혹은 sucrose 9% 2,4-D 0.01 mg/L에서 자구분화가 양호하였으며, 2,4-D 0.1 mg/L의 고농도가 되면 오히려 억제적이였다. BA 첨가는 대조구에 비해 자구분화는 촉진적이었으나 자구비대는 불량하였다. 기내자구 소인 편배양에서 생장조절제를 무첨가 시, NH$_4$NO$_3$를 1/2로 줄인 MS배지에서 배양할 경우 자구비대에 효과적이고 sucrose농도가 12%일 때 자구비대가 양호하였다.

• 식물조직배양학회지
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• 제27권6호
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• pp.447-451
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• 2000

나리‘Gelria’의 인편에서 캘러스를 유도, 증식시키고, 증식된 캘러스에서 식물체를 재분화하여 캘러스를 통한 재분화 기술체계를 확립하고자 실험한 결과, 인편에서 캘러스의 유도는 생장조절제가 전혀 첨가되지 않은 배지에서도 유도되었고, kinetin 0.5 ∼ l.0 mg/L와 NAA 0.1 ∼ l.0 mg/L가 첨가된 배지에서는 유연성이 높은 캘러스가 100%유도되었다. 생장조절제가 첨가되지 않은 배지에서도 캘러스의 증식이 양호하였으며, 생장조절제가 첨가된 배지에서는 증식률이 높아 캘러스가 왕성하게 증식되었다. 또한 캘러스로부터 신초가 발생하여 캘러스의 증식과 신초의 재분화가 동시에 발생하였다. 캘러스는 암배양보다는 명배양에서, 액체배양보다는 고체배양에서 더 잘 증식하였다. 생장조절제가 첨가된 배지에서는 캘러스가 왕성하게 증식하였고 신초의 재생은 생장조절제가 첨가되지 않은 배지에서 높았고, 다음으로 1.0mg.L의 BA와 NAA가 첨가된 배지에서 높았다.