• Journal of Bio-Environment Control
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• v.26 no.2
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• pp.49-55
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• 2017

The objective of this research was to investigate the effects of planting date, root pruning and uprooting on growth, yield and budding ratio of 'Seolhyang' strawberry (Fragaria${\times}$ananassa Duch.). Planting dates of plantlets with 60 ~ 70 days old were September 7th, September 14th and September 21th. Root pruning rates were controlled to 25% (RP 25) or 50% (RP 50) before transplanting. In the uprooting treatments, the plantlets were pulled out on root media and were replanted into same bed at 10 days (UR 10) and 20 days (UR 20) after transplanting. The delayed planting dates of plantlet resulted in the suppressed growth of plant, reduced yield, and slight earlier appearance of flower buds. The RP 50 treatment showed the lowest value among root pruning treatments in growth and yield, but appearance of flower buds slightly fall behinds. The UR 20 treatment only made flower budding earlier by 6 days than non-treatment. All of root stress treatment was appeared to decrease vegetative growth and yield except the RP 25 treatment. Above results imply that strong root stress was required to emerge second flower bud earlier but which reduced overall growth and yield in 'Seolhyang' strawberry.

• Journal of Korean Society of Forest Science
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• v.71 no.1
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• pp.9-13
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• 1985

Protoplasts were isolated from cotyledons of germinating Pinus densiflora S. et Z. seeds. The seeds were germinated for nine days, and excised embryonic cotyledons about 3-4mm long were incubated with Cellulase Onozuka R-10 and Macerozyme. After 8 hours of incubation, large number of viable protoplasts were isolated. Isolated protoplasts were cultured in a medium containing basal salts of $B_5$ medium, vitamines, amino acids, organic acid, sugars, and growth hormones. The first evidence of protoplast budding was observed after twelve hours in culture, and it suggested that high potential of the embryonic cotyledons for rapid cell division affected the early budding, rather than effect of culture medium was shown in twelve hours. The three- to four-cell stage was reached after three to four days of culture. Most cell divisions were achieved by additional buddings rather than equal binary cell division. No further cell division was observed beyond the four-cell stage. Protoplasts isolated from fully expanded cotyledons (germinated for 17 to 24 days) seldom initiated or failed to initiate cell division.

• Proceedings of the Korean Society of Crop Science Conference
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• 1997.10a
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• pp.84-85
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• 1997

• Bulletin of the Korean Space Science Society
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• 1996.11a
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• pp.9-9
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• 1996

No Abstract, See Full Text

• Journal of Veterinary Clinics
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• v.31 no.2
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• pp.156-158
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• 2014

Malassezia spp. was isolated from the skin lesion of a South American Sea Lion (Otaria byronia) in South Korea. Colonies were cream to yellowish with smooth margin on Sabouraud dextrose agar without lipid supplements. Yeast cells were ovoid to cylindrical in shape and budding daughter cells from broad base. We conducted 26s rRNA sequencing to specify the strain of the yeast and found out this isolate highly matched with Malassezia pachydermatis isolated from canine otitis. The pulse therapy of oral itraconazole was very effective in this case. M. pachydermatis has a wide range of host animals but this is the first report in a sea lion in South Korea.

• Archives of Plastic Surgery
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• v.50 no.1
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• pp.130-140
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• 2023

Microsurgery, which deservedly sits on top of the reconstruction ladder, has been a boon to plastic surgery. It is because of this marvelous tool that plastic surgeons the world over have been able to tackle many reconstructive dilemmas, which were once considered to be an improbability. Microsurgery-aided revolutions have rendered a new meaning to all forms of reconstruction-whether it is postoncological, posttraumatic, or postlymphedema reconstruction. As the most advanced reconstructive medium at our disposal that has broadened the horizons of plastic surgery exponentially, it is but obvious that many budding plastic surgeons are drawn toward this subspecialty. In lieu of the aforementioned facts, it is necessary to sensitize all such aspiring surgeons about the various intricacies concerning the field of microsurgery. This article with its focus on the six desirable microsurgical attributes of "Clarity, Curiosity, Perseverance, Passion, An Open Mindset and Action," is meant to be a modest attempt on part of the authors to share their microsurgical insights, procured through their respective journeys, with budding aspirants, hoping to sensitize as well as motivate them for the challenging path that lies ahead.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.2
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• pp.156-160
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• 2008

This study was conducted to find on the screening of a optimal variety after compare with characteristics of a colored soybean recommended and a vegetable soybean by pure line isolation from the indigenous variety in Chungnam area. The budding period in results compared with a budding property of testing varieties between the recommended colored soybean 6 varieties and the indigenous 3 varieties, was showed that it was delayed by increasing of a grain weight but the budding rate was not significant. And the harvest period was the most early day at the black colored soybean plot at the 30th July and was the latest day at the native variety (Nonsan jaerae) at the 21 th October. The stem length was showed a wide range from 25 to 104 cm, and the Asan jaerae in this result was the highest on 104 cm, but the Geomjeongolkong was the lowest on 25 cm. The number of pod per plant was searched about 9 to 111 range, and the most numerous plot in this experimental varieties was the Ilpumgeomjeongkong as 111 number, on the other hand, the least variety was the Asan jaerae as 9 number. The rate of more than 2 seed per pod in the vegetable soybean property was the highest at the Geomjeongolkong about 82%. Additionally, the Hwangkuemkong and Geomjeongkong 1 was relatively the high levels as 78% and 73%, respectively. At the pod yield per $1000\;m^2$ (10a) was the most much as 1,567 kg at the Ilpumgeomjeongkong. Also, the Sunheukkong and the Galmikong was 1,468 kg, 1,397 kg, respectively. Therefore, the Ilpumgeomjeongkong, Sunheukkong, and Galmikong were proper varieties for the vegetable cultivation.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.579-584
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• 2001

This experiment was conducted to investigate the effect of soil moisture content on cut flower quality of Gypsophila paniculata L. 'Bristol Fairy' in spring and autumn cultivations. The soil moisture potential (SMP) set point for irrigation was either -3.2 or -10.0kPa in the vegetative growth stage, and -10.0, -31.6, or -79.4kPa after the budding stage. When soil moisture reached at each set SMP point, 10 mm water was drip-irrigated. Tendency of flowering was earlier as SMP set point was lower in both vegetative growth and after budding stages. No other flower characteristics were found among other treatments. In both spring and autumn cultivations, as SMP set point was lower, the low primary branch length and upper internode length were decreased, the stem firmness was increased, and curvature of cut flower stem was decreased. Especially, in autumn, the higher the SMP set point, the higher the low primary branch than the apex, thus the paniculate inflorescence was deeply destroyed. Yield in spring cultivation was not significantly different among treatments, while that in autumn cultivation increased as the lower the SMP value. It is thought that optimal imgation points during vegetative growth and after budding stages to produce high quality cut flowers are -10.0 and -79.4kPa, respectively.

• Genomics & Informatics
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• v.16 no.2
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• pp.22-29
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• 2018

Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification of gene functions by growth fitness analysis. For fine tuning, it is important to examine barcode sequences, because mutations arise during strain construction. Out of 8,708 barcodes (4,354 strains) covering 88.5% of all 4,919 open reading frames, 7,734 barcodes (88.8%) were validated as high-fidelity to be inserted at the correct positions by Sanger sequencing. Sequence examination of the 7,734 high-fidelity barcodes revealed that 1,039 barcodes (13.4%) deviated from the original design. In total, 1,284 mutations (mutation rate of 16.6%) exist within the 1,039 mutated barcodes, which is comparable to budding yeast (18%). When the type of mutation was considered, substitutions accounted for 845 mutations (10.9%), deletions accounted for 319 mutations (4.1%), and insertions accounted for 121 mutations (1.6%). Peculiarly, the frequency of substitutions (67.6%) was unexpectedly higher than in budding yeast (~28%) and well above the predicted error of Sanger sequencing (~2%), which might have arisen during the solid-phase oligonucleotide synthesis and PCR amplification of the barcodes during strain construction. When the mutation rate was analyzed by position within 20-mer barcodes using the 1,284 mutations from the 7,734 sequenced barcodes, there was no significant difference between up-tags and down-tags at a given position. The mutation frequency at a given position was similar at most positions, ranging from 0.4% (32/7,734) to 1.1% (82/7,734), except at position 1, which was highest (3.1%), as in budding yeast. Together, well-defined barcode sequences, combined with the next-generation sequencing platform, promise to make the fission yeast gene deletion library a powerful tool for understanding gene function.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.226-230
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• 2009

To investigate the effect of night minimum temperature on growth and flower development in standard type chrysanthemum 'Baekina' during winter season, morphological characteristics and physiological disorders were compared with standard chrysanthemum 'Jinba'. Although the flower budding of 'Baekma' was delayed about 2 to the days compared with 'Jinba', the days to flowering of 54 to 59 showed no difference between the two cultivars. Flowering was delayed as minimum temperature decreased at night. The length and weight of cut flower decreased in the two cultivars as minimum temperature increased at night. In 'Baekina', the number of petals at 16 showed the highest values among other temperatures. Whereas there was no significant difference among the temperature treatments in 'Jinba'. Rosette occured only in 'Baekma', and the ratio was 11.1% at 14, 55.6% at 16, and 56.1% at 18. The inhibition of flower budding appeared only at 14 in the ratio of 17.3% in 'Baekma' and 4.0% in 'Jinba'.