• 대한예방한의학회지
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• 제13권2호
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• pp.103-113
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• 2009

This study was performed to evaluate the effect of HCB on the bone mass and its related factors in estrogen-deficient animal model. The model rats of osteoporosis showed a significant decrease in bone density, bone ash density, calcium content of femur bone. At the 14th day after ovariectomy-surgery, rats were administered with HCB, extract of Houyttnia cordata, per orally, and continued for 10 weeks. And osteoporosis related parameters were determined to investigate the effect of HCB. Osteoporotic rats showed lower serum estrogen level, higher body weight than normal rats, and showed atrophy of uterine horns.

• Molecules and Cells
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• 제26권5호
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• pp.486-489
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• 2008

Curcumin (diferuloylmethane), a pigment derived from turmeric, has anti-oxidant and anti-inflammatory activities. Accumulating evidence points to a biochemical link between increased oxidative stress and reduced bone density. Osteoclast formation was evaluated in co-cultures of bone marrow stromal cells (BMSC) and whole bone marrow cells (BMC). Expression of receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL) was analyzed at the mRNA and protein levels. Exposure to curcumin led to dose-dependent suppression of osteoclastogenesis in the co-culture system, and to reduced expression of RANKL in $IL-1{\alpha}$-stimulated BMSCs. Addition of RANKL abolished the inhibition of osteoclastogenesis by curcumin, whereas the addition of prostaglandin $E_2$ ($PGE_2$) did not. The decreased osteoclastogenesis induced by curcumin may reduce bone loss and be of potential benefit in preventing and/or attenuating osteoporosis.

• 대한한방부인과학회지
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• 제19권1호
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• pp.188-201
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• 2006

Purpose : This study was conducted to investigate the effect of pregnancy and parity on bone marrow density using Dual Energy X-ray Absorptiometry (DXA) after parturition Methods : The observation cases who was over 20 and under 35 years old just after childbirth were admitted twice to woosuk university hospital from Aug 2000 to July 2005. During the first admission period, we measured the bone marrow density(BMD) using DXA in 13th day, and when the patient came to the hospital just after childbirth again, we followed up the BMD in 13th day. The evaluation index of this report was comparison of the T-score which was about the lumbar spine(L1-L4) BMD and femur neck BMD. Results : The continuous parturition was increased the lumbar spine BMD(P<<0.05), and decreased the femur neck BMD but it had no meaning. In the cases of the parturition interval under 24 months regarded as siblings born within a year of each other, the analysis results of BMD showed increase in lumbar spine BMD. In the cases of the parturition interval over 24 months, there was increased in lumbar BMD, and decreased in femur neck BMD. To the analysis of the weight variation, the increased BMI group has a significant increase in lumbar spine BMD, and the decreased BMI group also increase in lumbar spine BMD but there was no meaning about that. Conclusion : The continuous parturition was increased the lumbar spine BMD.

• 한국수정란이식학회지
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• 제23권2호
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• pp.127-131
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• 2008

Bone marrow (BM) cell harvesting is a crucial element in the isolation of mesenchymal stem cells (MSCs). A simple method for harvesting cat BM cells is described. The results show that a large number of BM cells can rapidly be harvested from the cat by this simple procedure. MSCs prepared by density-gradient method were spindle-shaped morphology with bipolar or polygonal cell bodies and strongly positive for CD9 and CD44 and negative for CD18 and CD45-like. They were capable of differentiation to adipocytic and osteocytic phenotypes when exposed to appropriate induction media. The advantages of this method are its rapidity, simplicity, low invasiveness, and low donor attrition and good outcome.

• 한국식품영양학회지
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• 제35권5호
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• pp.295-301
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• 2022

The medicines for treating osteoporosis currently in use have minor to severe side effects, and can be financially burdensome. Thus, there is a need for prevention and alternative supplement that is relatively inexpensive, and can be easily consumed daily as an alternative dietary therapy. In this study, bone marrow density of the spine and femur of osteoporosis patients were checked before and after consuming complex composed of calcium and magnesium, considered to be the core of bone mineral content. November 2017-November 2021, patients with T-score of less than -2.5 or -1.0 < T-score < -2.5 with history of fractures or recent fractures were enrolled. The data of 60 patients who orally administered Ionized Cal/Mag^TM Complex were reviewed retrospectively, and it was significantly confirmed that the average value of T-score was up-regulated by 0.5. Additionally, the cumulative dose was observed to have a positive effect, on the improvement of BMD in the 2nd Lumbar and Femur neck. It is expected that better results will be achieved if use of the supplement is continued.

• Applied Microscopy
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• 제32권3호
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• pp.223-230
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• 2002

Sprague-Dawley rat에 complete Freund's adjuvant (CFA)를 투여하여 유발한 골다공증에 미치는 $1{\alpha}$, 25 dihydroxyvitamin $D_3\;(Vit\;D_3)$의 효과를 알아보기 위하여 실험동물을 정상 대조군, CFA 투여 대조군, CFA 투여후 $1{\alpha}$, 25 dihydroxyvitamin $D_3\;0.01{\mu}g/kg$ 투여군 (Vit $D_{3}L$) 및 $0.1{\mu}g/kg$ 투여군(Vit $D_{3}H$)으로 분류하여 경구로 3주간 투여하였다. 골다공증의 억제 효과를 알아보기 위하여 골밀도와 골함유량의 변화 및 주사전자현미경적 소견을 관찰한 결과 다음과 같은 결론을 얻었다. 골밀도와 골함유량은 $1{\alpha}$, 25 dihydroxyvitamin $D_3$ 투여용량과 부위에 따라 차이가 있었는데 Vit $D_3$ 투여군이 CFA 투여 대조군과 비교하여 골밀도와 골함유량이 증가하였으며 Vit $D_{3}H$군이 Vit $D_{3}L$군보다 좀 더 효과적이었다. 대퇴골의 주사전자현미경적 소견은 Vit $D_3$ 투여군의 골피질과 골소주의 손상이 CFA 투여 대조군보다 억제되었음을 볼 수 있었으며 Vit $D_{3}H$ 투여군이 Vit $D_{3}L$ 투여군보다 좀 더 효과적이었다. 이상의 결과를 종합해 보면 $1{\alpha}$, 25 dihydroxyvitamin $D_3$의 투여는 CFA에 의해 유발된 rat의 골다공증 모델에서 골다공증의 진행을 억제하였고 Vit $D_{3}H$ 투여군이 Vit $D_{3}L$ 투여군보다 좀 더 효과적이었다.

• Journal of Korean Dental Science
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• 제7권1호
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• pp.16-24
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• 2014

Purpose: This study was planned to clarify a negative view of chewing gum due to the concern that continuous gum chewing might cause a change in the gonial angle and make the lower facial appearance look square. Materials and Methods: We had 25 adults (13 males and 12 females, with an average of 27.3 years) chew 6 g of gum (spearmint) evenly with both right and left posterior teeth for one hour per day for three months. We then measured their gonial angle, the inclination of occlusal plane, facial height, bone marrow density, and masticatory force before chewing, 1, 2, and 3 month after chewing to verify its significance statistically. Result: The results showed that the gonial angle increased from $122.7^{\circ}$ to $123.3^{\circ}$ (P>0.05), and thus the jaws became slightly slimmer. There was no change in the occlusal plane inclination and facial height. Meanwhile bone marrow density in the mandibular angle and ascending ramus increased from $0.285g/cm^2$ to $0.290g/cm^2$ (P<0.05), and masticatory force also increased by 0.5 kg on the right side and 0.8 kg on the left side (P<0.05). Conclusion: Continuous chewing of gum gives an appropriate exercise effect to the stomatognathic system. As chewing gum has effect on increase bone marrow density without changing the mandibular angle and facial appearance the claim that jaw bone changes to a square jaw through chewing gum is regarded to be groundless.

• 한국방사선학회논문지
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• 제11권1호
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• pp.43-48
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• 2017

최근 동위원소를 이용한 뼈 스캔 (Bone Scan)검사 후 골밀도 (BMD; Bone Mineral Density)검사를 당일검사로 병행한 경우 이로 인한 골밀도 측정값에 오차 발생 가능성이 제기되고 있으나 방사성의약품 표지화합물 투여 후 이중에너지 X선을 이용한 골밀도 측정값 변화에 대한 임상적 자료가 미비하여 핵의학 체내검사 후 당일 골다공증 검사의 측정값에 대한 논란의 소지가 있다. 따라서 동위원소 표지화합물인 $^{99m}Tc-MDP$가 골밀도 측정값에 영향을 미치는지 임상적 측면에서 실험하였다. 실험에 참가한 대상자의 평균 나이는 $35.17{\pm}9.45$세로 실험 대상자 17명 중 대사성 질환과 골밀도 측정에 영향을 줄 수 있는 허리뼈 압박골절 및 몸쪽 넓적다리뼈 골절이 있는 자를 제외한 12명 중 정상 골밀도 T-scores>-1.0의 환자 6명을 대상으로 $^{99m}Tc-MDP$ 투여 전 후 측정값을 분석한 결과 허리뼈에서 전 후 각각 평균 $0.975{\pm}0.084g/cm^2$, $0.966{\pm}0.078g/cm^2$으로 $0.009g/cm^2$ 증가, 우측 몸쪽 넓적다리뼈에서는 전 후 각각 평균 $0.909{\pm}0.078g/cm^2$, $0.913{\pm}0.086g/cm^2$으로 $0.004g/cm^2$ 감소, 좌측 몸쪽 넓적다리뼈에서는 각각 평균 $0.887{\pm}0.099g/cm^2$, $0.881{\pm}0.103g/cm^2$으로 $0.007g/cm^2$의 증가를 보여 몸쪽 넓적다리뼈 보다 허리뼈에서 더 큰 골밀도 변화를 보여주었다. 그러나 허리뼈와 몸쪽 넓적다리뼈 전체에서 전 후 변화 평균은 $0.0038{\pm}0.014g/cm^2$으로 골밀도 측정값에 유의한 영향이 없음을 알 수 있으며, 또한 두 실험간 전체 상관계수는 0.987으로 방사성동위원소 표지화합물인 $^{99m}Tc-MDP$ 투여가 골밀도 측정값에 영향을 주지 않았다. 따라서 140 keV의 감마선 에너지를 방출하는 테크네슘 표지화합물을 이용한 뼈 스캔검사 후 골밀도 측정값에 유의한 영향을 미치지 않음을 확인하였다. 그러나 핵의학적 체내검사와 골다공증 검사를 당일로 검사함으로 인한 환자의 피폭을 고려한다면 시간 간격을 두고 검사를 시행하는 것이 좋을 것으로 사료된다.

• The Journal of Advanced Prosthodontics
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• 제6권2호
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• pp.96-102
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• 2014

PURPOSE. This study was performed to characterize the effects of zirconia coated with calcium phosphate and hydroxyapatite compared to smooth zirconia after bone marrow-derived osteoblast culture. MATERIALS AND METHODS. Bone marrow-derived osteoblasts were cultured on (1) smooth zirconia, (2) zirconia coated with calcium phosphate (CaP), and (3) zirconia coated with hydroxyapatite (HA). The tetrazolium-based colorimetric assay (MTT test) was used for cell proliferation evaluation. Scanning electron microscopy (SEM) and alkaline phosphatase (ALP) activity was measured to evaluate the cellular morphology and differentiation rate. X-ray photoelectron spectroscopy (XPS) was employed for the analysis of surface chemistry. The genetic expression of the osteoblasts and dissolution behavior of the coatings were observed. Assessment of the significance level of the differences between the groups was done with analysis of variance (ANOVA). RESULTS. From the MTT assay, no significant difference between smooth and surface coated zirconia was found (P>.05). From the SEM image, cells on all three groups of discs were sporadically triangular or spread out in shape with formation of filopodia. From the ALP activity assay, the optical density of osteoblasts on smooth zirconia discs was higher than that on surface treated zirconia discs (P>.05). Most of the genes related to cell adhesion showed similar expression level between smooth and surface treated zirconia. The dissolution rate was higher with CaP than HA coating. CONCLUSION. The attachment and growth behavior of bone-marrow-derived osteoblasts cultured on smooth surface coated zirconia showed comparable results. However, the HA coating showed more time-dependent stability compared to the CaP coating.

• Journal of Korean Neurosurgical Society
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• 제48권2호
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• pp.125-128
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• 2010

Objective : The purpose of this study was to determine the optimal volume of injected cement and its distribution when used to treat vertebral compression fractures, and to identify factors related to subsequent vertebral fractures. Methods : A retrospective analysis of newly developing vertebral fractures after percutaneous vertebroplasty was done. The inclusion criteria were that the fracture was a single first onset fracture with exclusion of pathologic fractures. Forty-three patients were included in the study with a minimum follow up period of six months. Patients were dichotomized for the analysis by volume of cement, initial vertebral height loss, bone marrow density, and endplate-to-endplate cement augmentation. Results : None of the four study variables was found to be significantly associated with the occurrence of a subsequent vertebral compression fracture. In particular, and injected cement volume of more or less that 3.5 cc was not associated with occurrence (p = 0.2523). No relation was observed between initial vertebral height loss and bone marrow density (p = 0.1652, 0.2064). Furthermore, endplate-to-endplate cement augmentation was also not found to be significantly associated with a subsequent fracture (p = 0.2860) by Fisher's exact test. Conclusion : Neither volume of cement, initial vertebral height loss, bone marrow density, or endplate-to-endplate cement augmentation was found to be significantly related to the occurrence of a subsequent vertebral compression fracture. Our findings suggest that as much cement as possible without causing leakage should be used.