Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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A Simple Method for Cat Bone Marrow-derived Mesenchymal Stem Cell Harvesting

  • Jin, Guang-Zhen (Division of Applied Life Science (BK21)) ;
  • Lee, Young-Soo (Division of Applied Life Science (BK21)) ;
  • Choi, Eu-Gene (Division of Applied Life Science (BK21)) ;
  • Cho, Kyu-Woan (Department of Veterinary internal Medicine, Institute of Animal Medicine, College of Veterinary Medicine, Graduate School of Gyeongsang National University) ;
  • Kong, Il-Keun (Division of Applied Life Science (BK21))
  • Published : 2008.06.30
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Abstract

Bone marrow (BM) cell harvesting is a crucial element in the isolation of mesenchymal stem cells (MSCs). A simple method for harvesting cat BM cells is described. The results show that a large number of BM cells can rapidly be harvested from the cat by this simple procedure. MSCs prepared by density-gradient method were spindle-shaped morphology with bipolar or polygonal cell bodies and strongly positive for CD9 and CD44 and negative for CD18 and CD45-like. They were capable of differentiation to adipocytic and osteocytic phenotypes when exposed to appropriate induction media. The advantages of this method are its rapidity, simplicity, low invasiveness, and low donor attrition and good outcome.

Keywords

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