• Journal of Yeungnam Medical Science
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• 제8권2호
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• pp.106-113
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• 1991

저자들은 1983년 5월부터 1990년 2월까지 영남대학병원 내과에서 다발성 골수종으로 진단 되었던 총 31예의 환자를 대상으로 임상적 고찰을 실시하여 다음과 같은 결과를 얻었다. 1. 연령적으로 60대(45%)에서 가장 많았으며 남녀비는 1.8 : 1로 남성 우위를 보였다. 2. 진단당시 주증상으로는 골통증(58%)이 가장 많았고, 발열, 호흡곤란, 현기증, 종물촉지 등이 있었다. 3. Southwest Oncology Group(SWOG)의 진단기준에 따른 검사소견의 분포는 골이나 연부조직의 생검상 골수종을 보인 예가 6예, 골수검사상 총유핵세포 중 형질세포의 비율이 10%이상인 예가 22예, 혈청단백 전기영동상에서 Monoclonal splike를 보인 예가 24예, X-선검사상 골붕괴소견을 보인 예가 22예에서 관찰되었다. 4. 병기분류에 따른 환자의 분포는 stage I이 2예, stage II가 3예, stage III가 26예로 전체 대상환자의 84%였다. 5. 면역전기영동검사결가 IgG가 19예로 64%로 가장 많았으며, Light chain이 7예로 23%, IgA가 3예로 10% 순이었다. Light chain중 Kappa와 Lambda의 비는 1.1 : 1로 거의 비슷하였다. 6. 혈액학적소견상 혈색소치가 8.5g%미만이 42%를 차지하였고, 10.6mg%이상의 고칼슘혈증을 보인 환자는 22%였고, 혈청 creatinine치의 경우 2.0mg%이상이 19%를 차지하였다. 7. 골 X-선학적 검사소견상 다발성 골용성 병변은 두개골에 65%로 가장 많았고, 다음이 늑골로 42%, 요추 35%, 골반 23%, 흉추 순으로 관찰되었다. 골조송증과 골붕괴 및 골절이 동반된 예가 55%, 골붕괴만 보인 예가 23%, 골조송증만 보인 예가 10%였다. 8. 합병증으로는 신기능 장애가 10예, 감염이 8예, 척추의 압박골절이 16예에 관찰되었다.

• Childhood Kidney Diseases
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• 제8권1호
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• pp.43-50
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• 2004

목 적 : 신 사구체 질환을 포함한 여러 질환에서 스테로이드가 장기간 투여되고 있다. 스테로이드의 장기간에 걸친 복용은 여러 가지의 부작용을 초래한다. 그중 골다공증은 성장기의 어린이들에는 심각한 부작용 중에 하나이다. 저자들은 신 사구체 질환으로 지속적인 스테로이드 투여를 진행하면서 위약과 pamidronate를 복용시켜 pamidronate의 골다공증 예방효과를 알아보고자 하였다. 이중 에너지 X선 흡수법(dual energy X-ray absorptiometry, DEXA)과 골대사의 생화학적 지표들을 이용하여 연구하였다. 방 법 : 본 연구는 혈뇨와 단백뇨가 동반되어 2003년 8월부터 2004년 2월 사이에 경희대학교 의과대학 부속병원 소아과에서 지속적인 스테로이드 투여를 3개월 이상 시행 중인 44명(M : F=25: 19)의 환아들을 대상으로 하였다. 대상 환아 44명 모두에게는 하루 500 mg의 칼슘제재가 공급되었고, 이 가운데 16명에게는 pamidronate(125 mg/cap)를 하루 1 cap씩 투여하였고, 다른 28명에게는 위약을 3개월간 투여하였다. 지속적인 스테로이드 투여를 시행 중 3개월 사이의 AST, ALT, 요소 질소, 크레아티닌, 칼슘과 인의 농도의 변화를 측정하였다. 골밀도의 변화는 이중 에너지 X-선 흡수법을 이용하여 측정하였고, 부갑상선 호르몬(PTH), 혈중 osteocalcin, 요중 dipyridinoline의 변화를 조사하였다. 결 과 : 골밀도 검사 상 대조군에서는 $0.754{\pm}0.211(g/cm^2)$에서 3개월 동안의 스테로이드 치료 후 $0.728{\pm}0.208(g/cm^2)$로 통계상 유의한 정도의 골밀도 감소를 보였다(P=0.0004). 반면 pamidronate를 투여한 그룹에서는 지속적인 스테로이드 치료를 받은 3개월 동안 골밀도가 $0.652{\pm}0.194(g/cm^2)$에서 $0.658{\pm}0.226(g/cm^2)$로 증가하였다. 비록 통계학적으로 유의한 증가 소견은 보이지 않았지만 대조군에 비해 스테로이드 치료에 의한 골밀도의 지속적인 저하를 예방하는 효과가 있음을 보여주었다. 결 론 : 장기 스테로이드 치료요법을 시행받는 환아에서 pamidronate 같은 biphosphonates 제재가 골밀도 저하를 예방하는데 유용하게 사용될 수 있을 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권10호
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• pp.1479-1487
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• 2015

A total of 420 day-old male Ross chicks were weighed at d 1 of life and assigned to test diets to assess the efficacy of a new Buttiauxella spp. phytase expressed in Trichoderma reesei. Diets were: positive control (PC) adequate in nutrients and negative control (NC) diet (40% and 17% less available phosphorous (P) and calcium (Ca), respectively) supplemented with 6 levels of phytase 0, 250, 500, 750, 1,000, and 2,000 phytase units (FTU)/kg of diet. All diets had titanium dioxide as digestibility marker and each diet was allocated to ten cages (6 birds/cage). Diets were fed for 3 wk to measure growth performance, apparent retention (AR) on d 17 to 21 and bone ash and ileal digestibility (AID) on d 22. Growth performance and nutrient utilization was lower (p<0.05) for NC vs PC birds. Phytase response in NC birds was linear (p<0.05) with 2,000 FTU showing the greatest improvement on body weight gain (20%), feed conversion (7.4%), tibia ash (18%), AR of Ca (38%), AR of P (51%) and apparent metabolizable energy corrected for nitrogen (5.1%) relative to NC. Furthermore, phytase at ${\geq}750FTU$ resulted in AID of total AA commensurate to that of PC fed birds and at ${\geq}1,000FTU$ improved (p<0.05) AR of P, dry matter, and N beyond that of the lower doses of phytase and PC diet. In conclusion, the result from this study showed that in addition to increased P and Ca utilization, the new Buttiauxella phytase enhanced growth performance and utilization of other nutrients in broiler chickens in a dose-dependent manner.

• Childhood Kidney Diseases
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• 제21권1호
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• pp.21-25
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• 2017

Severe hypercalcemia is rarely encountered in children, even though serum calcium concentrations above 15-16 mg/dL could be life-threatening. We present a patient having severe hypercalcemia and azotemia. A 14-year-old boy with no significant past medical history was referred to our hospital with hypercalcemia and azotemia. Laboratory and imaging studies excluded hyperparathyroidism and solid tumor. Other laboratory findings including a peripheral blood profile were unremarkable. His hypercalcemia was not improved with massive hydration, diuretics, or even hemodialysis, but noticeably reversed with administration of calcitonin. A bone marrow biopsy performed to rule out the possibility of hematological malignancy revealed acute lymphoblastic leukemia. His hypercalcemia and azotemia resolved shortly after initiation of induction chemotherapy. Results in this patient indicate that a hematological malignancy could present with severe hypercalcemia even though blast cells have not appeared in the peripheral blood. Therefore, extensive evaluation to determine the cause of hypercalcemia is necessary. Additionally, appropriate treatment, viz., hydration or administration of calcitonin is important to prevent complications of severe hypercalcemia, including renal failure and nephrocalcinosis.

• Toxicological Research
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• 제33권1호
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• pp.49-54
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• 2017

Vitamin D3 is a fat-soluble secosteroid responsible for enhancing intestinal absorption of calcium, iron, and other materials. Vitamin D3 deficiency, therefore, can cause health problems such as metabolic diseases, and bone disorder. Female sex hormones including estrogen and progesterone are biosynthesized mainly in the granulosa cells of ovary. In this study, we isolated granulosa cells from porcine ovary and cultured for the experiments. In order to examine the effect of vitamin D3 on the ovarian granulosa cells, the mRNA and protein levels of genes were analyzed by real-time PCR and Western blot assay. The production of estrogen from the granulosa cells was also measured by the ELISA assay. Genes associated with follicle growth were not significantly altered by vitamin D3. However, it increases expression of genes involved in the estrogen-biosynthesis. Further, estrogen concentrations in porcine granulosa cell-cultured media increased in response to vitamin D3. These results showed that vitamin D3 is a powerful regulator of sex steroid hormone production in porcine granulosa cells, suggesting that vitamin D deficiency may result in inappropriate sexual development of industrial animals and eventually economic loss.

• 대한소아치과학회지
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• 제27권1호
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• pp.108-112
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• 2000

저인산혈증성 구루병은 구루병 중에서 보통 용량의 비타민 D 투약 치료에 반응하지 않는 형태로, 성염색체 우성 유전되며, 신세관에서 유기인의 재흡수가 감소되어 생기는 것으로 생각된다. 따라서, 저인산혈증이 특징적 소견이며, 혈청 내 알카리성 인산효소 활성이 증가되어 있고, 혈청 칼슘 농도는 보통 정상 범주에 속한다. 치과적 소견으로 특징적인 것은 임상적으로 건전한 치아에서 나타나는 다수의 자발적 치근단 농양과 농루이며, 치아의 맹출지연과 법랑질 저형성증도 관찰할 수 있다. 치과 방사선적으로는 치수각이 현저히 신장되어 있고, 때로는 상아법랑경계까지 연장되어 있으며, 치아주위 치조백선의 약화나 상실, 비정상적 치조골 소주 양상을 관찰할 수 있다. 본 환아는 저인산혈증성 구루병으로 진단되어 투약 치료 중이며, 구강검사 결과 특징적 치과소견인 다수의 자발적 치근단농양과 농루, 치수각의 현저한 신장 및 치아 맹출지연이 관찰되어 이의 구강 및 방사선 소견에 대해 보고하는 바이다.

• 대한의용생체공학회:의공학회지
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• 제25권4호
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• pp.309-314
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• 2004

본 연구에서는 세포배양을 위해 개발된 복합생물반응기(Hybrid bioreactor)의 배양조건의 유효성을 평가하고, 3차원 키토산 지지체 (Chitosan-Scaffold Sponge Type)에 배양된 세포에 Hybrid Bioreactor를 이용한 물리적 자극에 대한 반응을 실험하였다. Hybrid bioreactor는 압축변형과 전단변형을 동시에 가할 수 있도록 제작되었다. 본 실험에서는 지지체 크기의 2.5% 변형으로 14일간 150회씩 0.5Hz 로 물리적 자극을 가하였다. 14일간 배양한 세포군은 일정 날짜에 샘플링 (sampling) 하였다. (Day 6, 8, 10, 12, 14). 세포 성장 정도를 알 수 있는 전체 단백질 양을 Lowey의 방범으로 분석하였으며, 분화의 시작을 알리는 표적단백질인 알카라인 포스파타제(Alkaline phosphatase)양을 ELISA로 측정하였다. Hybrid bioreactor를 이용하여 물리적 자극을 가한 군은 자극을 가하지 않은 군에 비하여 표적단백질의 형성을 촉진하였으며, 자극을 가한 관에서 사극을 가하지 않은 군에 비해 빠른 칼슘침착을 나타내었다.

• Journal of Nutrition and Health
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• 제31권3호
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• pp.315-323
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• 1998

This study was performed to investigate the serum lipid levels and fatty acid composition in middle-aged men and the influence of cardiovscular risk factors on them. Data for dietary intake, anthropometry , blood pressure, and general environmental factors were obtained and their relationship to serum lipid levels and fatty acid composition were analyzed in 303 healthy male subjects with a mean age of 43.8$\pm$6.4 years from June 1 to 22 , 1995. The prevalence of obesity was 26.7% by BMI and 11.2% by WHR criteria . The percentage of hypertensive subjects were 13.5% and 22.4% by systolic and diastolic blood pressure, respectively. Retinol, calcium , and roboflavin intakes were lower than the RDA for Koreans. The ratio of carbohydrate intake to total calories of the subjects was 70%. Ratios of P/M and n-6/n-3 were 1.02/1.05/1.00 and 11.1/1, respectively. The percentage of subjects who had serum TG, TC, LDL-C leves beyond the normal range were 19.5% , 13.5% and 8.6% respectively. Palmitic comprised 26.4% of serum total fatty acids, linoleic acid 19.4% and oleic acid 16.4. The waist circumference and ration of carbohydrate intake were related positively to TG level. Serum cholesterol level had positive relations with waist circumference, DBP, sugar intake, and income. There were positive relationships between SFA and bone fishes and vegetable oil intakes, MUFA and DBP while a negative a negative relationship was observed between PUFA and animal fat intake. Serum n-6 fatty acid was related negatively to carbohydrate intake and hot taste preference. N-3 fatty acid was related positively to fish intake and negatively to egg intake. These results suggest that to maintain adequate serum lipid levels and fatty acid composition, sugar, animal fat, and carbohydrate intakes have to be reduced, while consumption of vegetable oil and fish must be increased. It is also essential to control abdominal fat accumulation and blood pressure.

• 동아시아식생활학회지
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• 제13권5호
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• pp.408-424
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• 2003

The purpose of this study was to analyze the nutritional status and dietary habits in predialysis patients of chronic renal failure(CRF). The patients group was composed of total 35 persons with chronic renal failure(diabetes exclusion), male 20, female 15 who were treated in the kidney internal department and the control group also composed of 35 persons, male 18, female 17 who were classified as normal by the medical examination. Their dietary habits, nutritional status and nutritional knowledge were investigated from two general hospitals in Inchon, middle of this year 2002. There were 31.4% of low weight patients (BMI below 20), 77.1% of anemia patients (serum hemoglobin below 12g/㎗), 6S.6% of hypertension patients with diastolic blood pressure over 90mmHg, 80% with systolic blood pressure over 140mmHg, 20% of hypercholesterolemia patients (serum cholesterol over 230mg/㎗), and 22.9% of hyperlipemia patients (serum triglyceride over 200mg/㎗). The cardiovascular disease seemed to be caused by the abnormality of lipid metabolism. The possibility of the bone disease was shown from patients of hyperphosphatemia (serum phosphorus over 4.7mg/㎗, 22.9%) and hypocalcemia (serum calcium below 8.4mg/㎗, 25.7%). Intake of insufficient calories which was caused by the lack of appetite affected on the nutritional status. The intake of most nutrients was not significantly different from the RDA for Koreans. Consequently, the patient groups took a lot of salt even after the diagnosis of CRF. But patients ate 6.lg of salt which were more than the recommended amount 2∼4g for patients with CRF. The patient groups, who had the experiences of nutritional counselling, had significantly higher nutritional knowledge related to CRF than control group. Unfortunately, patients could not have enough chances for nutritional counselling by the nutritionist even though they needed the nutritional informations and dietetic treatments. The continuous research is expected with regard to the detail plan for the improvement of nutritional support and the nutritional counselling because it is important to decide the requirements of nutrients for patients with kidney disease, considering the kidney function and status of nutrition.

• Journal of Periodontal and Implant Science
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• 제50권5호
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• pp.291-302
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• 2020

Purpose: The objective of this study was to investigate whether phelligridin D could reduce glucose-induced oxidative stress, attenuate the resulting inflammatory response, and restore the function of human periodontal ligament cells (HPDLCs). Methods: Primary HPDLCs were isolated from healthy human teeth and cultured. To investigate the effect of phelligridin D on glucose-induced oxidative stress, HPDLCs were treated with phelligridin D, various concentrations of glucose, and glucose oxidase. Glucose-induced oxidative stress, inflammatory molecules, osteoblast differentiation, and mineralization of the HPDLCs were measured by hydrogen peroxide (H₂O₂) generation, cellular viability, alkaline phosphatase (ALP) activity, alizarin red staining, and western blot analyses. Results: Glucose-induced oxidative stress led to increased production of H₂O₂, with negative impacts on cellular viability, ALP activity, and calcium deposition in HPDLCs. Furthermore, HPDLCs under glucose-induced oxidative stress showed induction of inflammatory molecules (intercellular adhesion molecule-1, vascular cell adhesion protein-1, tumor necrosis factor-alpha, interleukin-1-beta) and disturbances of osteogenic differentiation (bone morphogenetic protein-2, and -7, runt-related transcription factor-2), cementogenesis (cementum protein-1), and autophagy-related molecules (autophagy related 5, light chain 3 I/II, beclin-1). Phelligridin D restored all these molecules and maintained the function of HPDLCs even under glucose-induced oxidative stress. Conclusions: This study suggests that phelligridin D reduces the inflammation that results from glucose-induced oxidative stress and restores the function of HPDLCs (e.g., osteoblast differentiation) by upregulating autophagy.