• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.50 no.4
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• pp.447-454
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• 2014

The marine microalgae Chaetoceros calcitrans was cultured under a fluorescent lamp (CON) and light-emitting diodes (LEDs) of various wavelengths (blue, LB; red, LR; green, LG; white, LW); changes in growth, fucoxanthin, chlorophyll-a, amino acid and fatty acid profiles were investigated. LR-exposed cultures exhibited the highest specific growth rate (SGR) (0.34), whereas LG-exposed cultures showed the lowest SGR (0.26). After cultivation for 10 days, the maximum dry cell weight (g/L) of LR-exposed cultures was significantly higher than that of those exposed to other light conditions (LR${\geq_-}$CON>LB${\geq_-}$LW${\geq_-}$LG). Eicosapentaenoic acid (EPA) levels were significantly higher in CON-exposed cultures compared to those exposed to LW (P<0.05), with no marked difference compared to those exposed to LB, LR and LG (P>0.05). The fucoxanthin content was highest in LB-exposed cultures ($6.3{\mu}g/mL$), whereas LW showed the lowest ($3.6{\mu}g/mL$; P<0.05). Chlorophyll-a content was highest in cultures exposed to LB compared to other light sources. These results suggest consistent differences in growth and biochemical composition after exposure to light of different wavelengths.

• Journal of the Korean Society of Costume
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• v.61 no.5
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• pp.1-20
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• 2011

As a follow-up of a study on design strategies to improve Korea's Korean coast guarduniforms, this study set out to propose Korean coast guard uniforms of a new concept that would meet the changing needs of the times and match the advanced technology and work environments. Three concepts of classical, nautical and techno chic were identified from the design strategies of the previous study. Using the results from a prototype show and preference survey, the investigator divided items into design, color, material, and detail and reflects demands for each of them. As a result, design was examined in four aspects of identity, acknowledgement, unity, and practicality and focused its improvement factors on design differentiated from other organizations' uniforms including ground police, segmentation of size system, adjustment of the length of upper garment to consider activity, and changes to the lines and silhouette to give out a modern image. As for color, the focus was placed on differentiated colors from other organizations' uniforms including ground police through dark blue and ocean color, change toward colors that reflect environmental characteristics, and unity in colors between the summer and winter uniform. As for material, the focus of improvement was put on elasticity, resistance against contamination, warmth retention, texture, wearing sensation, and functionality by taking into account convenience for field workers on coast guard vessels and branch offices who have much time in contact with ocean environments in order to make the materials high-grade. As for detail, structural details were added to give out a modern image such as changing the detail lines, efficiency of storage space, buttons, sleeve hems, ironing lines, and neck collars by taking into account environmental characteristics. The significance of the study lies in that it proposed a development model for large-scale uniform copy design by establishing copy design that secures functionality and is proper for an organization's social and cultural environment.

• 한국해양학회지
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• v.7 no.2
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• pp.59-73
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• 1972

The heavy minerals of a barred estuarine and lagoonal sediments along the North Carolina coast have been studied with more than one hundred samples. Currents, salinity, and pH exhibit well-developed gradients from the upstream parts of the estuaries on the west toward the open ocean on the east. Twenty- four heavy minerals were identified in sediments of the study area. However, less than half of these occur frequently and the remainder exist only in minor quantities or trace amounts. Heavy minerals usually comprise less than 1% of the sample but vary from sample to sample. The maximum amount of heavy minerals in sediments of sounds and estuaries is 2.4% and in sediments of Outer Banks is 16.7%. Opaque minerals range from 10 to 85% of the total heavy mineral assemblage. Garnet and sillimanite are relatively more abundant in the eastern part than the western part in the area. Garnet more abundant in the northern part than the southern part, whereas sillimanite is more abundant in the southern part than the northern part, because the garnet source is in the northern part and one of the sillimanite source is in the southern part in the study area. The results of heavy mineral study indicate that the source of sediments is the Blue Ridge and Piedmont crystalline complex, and Coastal Plain formations. Some portions of sediments are transported from the Atlantic Ocean by the landward currents. They further indicate that the sediments of the Atlantic coast in the study area are transported mainly from the northern part to southern part by longshore littoral currents, and some portions of sediments are transported from the southern part to the northern part by the Gulf Stream.

• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1359-1367
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• 2005

The gene encoding Pyrobaculum arsenaticum DNA polymerase (Par DNA polymerase) was cloned and sequenced. The gene consists of 2,361 bp coding for a protein with 786 amino acid residues. The deduced amino acid sequence of Par DNA polymerase showed a high similarity to archaeal family B-type DNA polymerases (Group I), and contained all of the motifs conserved in the family B-type DNA polymerases for $3'{\rightarrow}5'$ exonuclease and polymerase activities. The Par DNA polymerase gene was expressed under the control of the T7lac promoter on the expression vector pET-22b(+) in Escherichia coli BL21-CodonPlus(DE3)-RP. The expressed enzyme was purified by heat treatment, and Cibacron blue 3GA and $Hirap^{TM}$ Heparin HP column chromatographies. The optimum pH of the purified enzyme was 7.5. The enzyme activity was activated by divalent cations, and was inhibited by EDTA and monovalent cations. The half-life of the enzyme at $95^{\circ}C$ was 6 h. Par DNA polymerase possessed associated $3'{\rightarrow}5'$ proofreading exonuclease activity, which is consistent with its deduced amino acid sequence. PCR experiment with Par DNA polymerase showed an amplified product, indicating that this enzyme might be useful in DNA amplification and PCR-based applications.

• Proceedings of the KSRS Conference
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• v.2
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• pp.905-908
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• 2006

The diffuse attenuation coefficient for downwelling irradiance $(K_d({\lambda}))$ is an important parameter for ocean studies. Based on the optical profile data measured during three cruises in the northern South China Sea in autumn from 2003 to 2005, variations in the $K_d({\lambda})spectra$ were analyzed. The variability of $K_d({\lambda})$ shows much distinct features both in magnitude and spectra shape. The $K_d({\lambda})value$ are much higher in costal waters than that of open oceanic waters and the blue-to-green(443/555) ratios of $K_d({\lambda})$ tends to increases with the chlorophyll a concentration ([Chl a]) from open ocean to coastal waters. These characteristics can be explained primarily by the increasing of $a_{w+p}(433)/a_{w+p}(555)$ with [Chl a]. In the short waveband, the relation between $K_d({\lambda})-K_w({\lambda})$ and [Chl a] can be well described by a power law function, suggesting the large contribution of phytoplankton to the variations in $K_d({\lambda})$. As for the spectral model of the diffuse attenuation coefficient, there are good linear relationships between $K_d(490)$ and $K_d({\lambda})$ at other wavelengths, with the slope parameter and the intercept following linear functions within the spectral range $412{\sim}555$ nm. These variabilities of $K_d({\lambda})$ provided much useful information for us to study the bio-optical properties in the northern South China Sea.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.51 no.4
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• pp.475-483
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• 2015

The marine microalgae Isochrysis galbana was cultured under various light-emitting diode (LED) light conditions with different wavelengths to examine changes in growth and in amino acid and fatty acid profiles. The culture conditions for the microalgae were Conway medium, salinity of 33 psu, temperature of $24^{\circ}C$, and a 16/8 h light/dark photoperiod. Six light sources, including 5 units of 180W LED lamps (peak wavelength: blue [LB] 470 nm; green [LG] 525 nm; yellow [LY] 595 nm; red [LR] 636 nm; white [LW] 442 nm) and 1 unit of a 175W metal halide (MH) lamp, were used for the experiment. The dry cell weights ($gL^{-1}$) of I. galbana under different light conditions were in the order of LW>LB${\geq}$MH>LR>LG>LY. Levels of essential amino acids were revealed to be significantly higher under LW, LG, and MH than under the other wavelengths (P<0.05). The fatty acid, unsaturated fatty acid, and DHA contents of I. galbana were higher under MH, LW, and LG. In addition, the carotenoid content was higher under MH, LW, and LG than under the other wavelengths (P<0.05). The fucoxanthin content was highest under MH (0.28%) and lowest under LY (0.2%), and it was 0.26% under LW and LG. The results indicate that the combined use of LW and LG is effective when using LED lamps for I. galbana cultivation.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.54 no.4
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• pp.315-323
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• 2018

Fossil fuel combustion during fishing activities is a major contributor to climate changes in the fishing industry. The Tier1 methodology calculation and on-site continuous measurements of the greenhouse gas were carried out through the use of fuel by the coastal and offshore gillnet (blue crabs and yellow croaker) and trap (small octopus and red snow crab) fishing boats in Korea. The emission comparison results showed that the field measurements are similar to or slightly higher than the Tier1 estimates for coastal gillnet and trap. In offshore gillnet and trap fisheries, Tier1 estimate of greenhouse gases was about $1,644-13,875kg\;CO_2/L$, which was more than the field measurement value. The $CO_2$ emissions factor based on the fuel usage was $2.49-3.2kg\;CO_2/L$ for coastal fisheries and $1.46-2.24kg\;CO_2/L$ for offshore fisheries. Furthermore, GHG emissions per unit catch and the ratio of field measurement and Tier1 emission estimate were investigated. Since the total catch of coastal fish was relatively small, the emission per unit catch in coastal fisheries was four to eight times larger. The results of this study could be used to determine the baseline data for responding to changes in fisheries environment and reducing greenhouse gas emission.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.24 no.3
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• pp.475-482
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• 2019

Over 3,200 large baleen whales including 3,173 fin whales, 7 blue whales and 28 humpback whales were hunted by Japanese whaling companies in the Yellow Sea during Japanese colonial period (1916~1944). As a result, these large baleen whales are under the endangered state in the Yellow Sea. The Yellow Sea had good living conditions for large baleen whales in the water temperature ($4{\sim}26^{\circ}C$) and food supply. The whaling on the large baleen whales was carried out mainly from early winter to late spring. The possibility of large scale whaling was caused by the migration of these baleen whales from other areas for the feeding on abundances of Euphausia pacifica in the surface layer during these seasons. During summer and autumn season, the baleen whales moved to other areas (good feeding ground as Woolsan offshore waters), because Euphausia pacifica stayed below the strong themocline which was formed from June to October in the Yellow Sea.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.6
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• pp.850-860
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• 2023

The effects of dietary mannan oligosaccharides, Lactobacillus plantarum, Bacillus subtilis, and Bacillus licheniformis supplementation on hybrid grouper Epinephelus akaara ♀×Epinephelus lanceolatus ♂ were evaluated. The fish were fed a basal diet and five other diets consisting of 0.6% mannan oligosaccharides, L. plantarum, B. subtilis, and B. licheniformis and mixture of each 0.15% prebiotic and all the probiotics (designated as MOS, LP, BS, BL, and SYN) for 56 days. Growth performance and feed utilization showed no significant differences among all experimental groups. Lipid level of whole-body was significantly high in MOS and BL groups. Plasma aspartate aminotransferase was significantly low in BL and SYN groups. Nitro-blue tetrazolium, lysozyme and anti-protease, and glutathione peroxidase in BS, SYN, and all probiotic groups, respectively, were significantly high. Intestinal Vibrio bacteria was significantly low in all probiotic and SYN groups. Gene expression of interleukin-1β and interleukin-10 in SYN group; transforming growth factor β2 in MOS and BS groups, toll-like receptor 2-2 in BS and BL groups; and C-type lectin in MOS, LP and SYN groups were significantly upregulated. Our findings indicate that mannan oligosaccharides, L. plantarum, B. subtilis, and B. licheniformis could improve innate immunity, antioxidant capacity, anti-inflammation, and intestinal microbiota of hybrid grouper.

• Korean Journal of Ichthyology
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• v.27 no.2
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• pp.149-152
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• 2015

A single specimen of Symphorus nematophorus (481.0 mm in SL), belonging to the family Lutjanidae, was firstly collected by a set net in the coastal waters of Geojae Island, located in south sea of Korea on August 1, 2014. This specimen was characterized by having the anterior dorsal soft rays produced into filaments, a pit between eye and nostril, no tooth on vomer, and nine longitudinal blue strips on both sides of the body. We propose a new Korean name, "Sil-tung-dom-sok" and "Sil-tung-dom" for the genus and species, respectively.