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Effect of black garlic on antioxidant activity and amino acids composition in Cheonggukjang (흑마늘 첨가에 따른 청국장의 항산화 및 아미노산의 변화)

  • Jeong, Tae-Seong;Kim, Jin-Hak;An, Shin-Ae;Won, Yong-Duk;Lee, Shin-Ho
    • Food Science and Preservation
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    • v.20 no.5
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    • pp.643-649
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    • 2013
  • The antioxidant activity and amino acid composition of various Cheonggukjang extracts, such as the water extract from Cheonggukjang (CWE), the alcohol extract from Cheonggukjang (CEE), the water extract from Cheonggukjang with black garlic (BWE) and the alcohol extract from Cheonggukjang with black garlic (BEE), were examined to investigate the effect of black garlic on the sensory quality and functional properties of Cheonggukjang. The total polyphenol contents of various extracts were 7.03 mg/100 g (BWE), 3.64 mg/100 g (CWE), 2.88 mg/100 g (BEE) and 0.81 mg/100 g (CEE). The radical scavenging activity of the DPPH radical was highest in BWE (91.83%), followed by BEE (37.35%), CWE (25.54%) and CEE (14.80%), in that order. The SOD-like activity was highest in BWE (20.20%), followed by BEE (9.22%), CWE (7.91%) and CEE (6.45%). The thiobarbituric acid reactive substances (TBARS) were highest in BWE (35.18%), followed by BEE (28.33%), CWE (17.40%) and CEE ((14.93%). The total amino acid content of Cheonggukjang (CC) was higher than that of Cheonggukjang with black garlic (CCWB), but the essential amino acid content of CCWB (43.18%) was higher than that of CC (42.27%). The 27 kinds of free amino acid were found in CC, but only 23 kinds were found in CCWB. The L-lysine content was highest (9.23%) in CC, and the L-phenylalanine content was highest (23.14%) in CCWB. The free amino acids (L-threonine, L-serine, L-sarcosine, L-proline, L-alanine, L-valine and D,L-${\beta}$-aminoisobutyric acid) were found in CC but not in CCWB. The ${\gamma}$-amino-n-butyric acid (GABA) was found in CCWB but not in CC. These results suggest that the addition of black garlic has beneficial effects on the functionality of Cheonggukjang without decreasing its sensory characteristics.

Water Uptake, Cotyledon Damage after Imbibition and Hypocotyl Elongation in Soybean with Different Seed Size and Color (콩 종실크기 및 종피색에 따른 침종후 수분흡수특성, 자엽손상 및 배축 신장력의 차이)

  • Park, Keum-Yong;Kim, Seok-Dong;Ryu, Yong-Hwan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.4
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    • pp.331-338
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    • 1994
  • The experiment was conducted to determine if seed size and seed coat color of soybean might be effective in water uptake and cotyledon damage after imbibition, and hypocotyl elongation. Eight soybean cultivars were separated into two classes of large and small seed based on seed weight, and each class included two cultivars with yellow and black seed color, respectively. Small seed size group was superior in water uptake by seed for 24 hour in imbibition at $25^{\circ}C$ , but its differences decreased as soaking time increased. Small seed cultivars germinated faster and had better germination rate than large ones. However, cultivars with black seed coat showed more slow water uptake at initial time and faster germination than yellow seed, but in 24 hour after imbibition, cultivars with black seed coat had higher water uptake rate than yellow seeds. Small seed cultivar group showed no cotyledon damage in imbibition for 24 hour while large seed cultivars were damaged 78% of cotyledon, and black seed showed low cotyledon damage compared to yellow seed. Hypocotyl length was shorter in large seed rather than in small seed, but hypocotyl thickness in large seed was more thick than in small seed. In correlation coefficients, seed coat rate, embryo rate exhibited significantly negative association with seed weight, and the correlation of seed weight with water uptake in 3 hour after soaking was significantly negative, but in 24 hour showed positive correlation.

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Development of an Air-Water Combined Cooling System (공냉-수냉 혼합냉각계통 개발)

  • Kwon, Tae-Soon;Bae, Sung-Won
    • The KSFM Journal of Fluid Machinery
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    • v.17 no.6
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    • pp.84-88
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    • 2014
  • A long term passive cooling system is considered as the most important safety feature for the nuclear design after the Fukushima Daiichi nuclear power plant accident in 2011. The conventional active pump driven safety systems are not available during a station Black Out (SBO) accident. The current design requirement on cooling time of the Passive Auxiliarly Feedwater System (PAFS) is about 8 hours only. To meet the 72 hours cooling time, the pool capacity of cooling water tank should be increased as much as 3~4 times larger than that of current water cooling tank. In order to extend the cooling time for 72 hours, a new passive air-water combined cooling system is proposed. This paper provides the feasibility of the combined passive air-water cooling system. The current pool capacity of water cooling system is preserved, and the cooling capability is extended by an additional air cooler.

Evaluation of Eco-Hydrological Changes in the Geum River Considering Dam Operations : II. Hydraulic Fish Habitat Condition Analysis (댐 운영을 고려한 금강의 생태.수문학적 변화 평가 : II. 수리학적 어류서식처 조건 분석)

  • Park, Sang-Young;Kim, Jeong-Kon;Ko, Ick-Hwan
    • Journal of Korea Water Resources Association
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    • v.42 no.5
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    • pp.407-414
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    • 2009
  • A quantitative analysis was conducted to study the impacts of artificial flow alteration on fish habitate condition change in the Geum River downstream the Daechung Multi-purpose Dam (DMD). River Analysis Package (RAP) was employed for the analysis and three fish species of black shiner, long nose barbel and Korean shinner were selected as icon species. The results of the analysis showed enhaced fish habitat conditions during low flow seasons in spring and fall after DMD construction, while the impact of the Youngdam Multipurpose Dam located upstream the DMD was insignificant. This result could be attributed to the fact that the increased flow during dry seasons helped create preferable habitat conditions for the fish species tested in this study.

A Study on the Four-Season Cooling Performance by Color of Water Proofing Membrane Materials Considering the View of Area (지역의 경관을 고려한 도막방수재의 색채별 사계절 차열 및 축열 성능에 관한 연구)

  • Ko, Jin-Soo;Kim, Byung-Yun
    • Journal of the Korean Institute of Rural Architecture
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    • v.17 no.2
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    • pp.9-16
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    • 2015
  • This study comparatively analyzed thermal characteristics of the green color, which is currently used the most, and other various colors of a rooftop urethane water proofing sheet. This study also analyzed the cooling performance by color of the water proofing sheet that fused cooling paints, and presented the effective water proofing sheet color for building energy savings. The experimental results are as follows: (1) The value of L (brightness) diminished, and brilliance also became lower from the white color to the black color, and thus, it was confirmed that relatively more heat was absorbed. In a and b chromaticity, which is the color attribute that ignores brightness, no special relationship was identified. (2) Considering that the cooling performance effect is bigger in summer than winter, due to heat reflection, the white water proofing sheet is more effective in building energy savings than the green water proofing sheet that is currently used the most. (3) The water proofing sheet's color has an impact more on cooling performance than the color of the background side of a structure on which water proofing sheet is installed. The experiment object of gray, of which background side is similar to cement mortar, was lower by $5.7^{\circ}C$ than the white background side.

Effect of Production In Vitro Embryo with Frozen-thawed Semen using AndroMed Extender in Korean Black Cow Semen (AndroMed를 이용한 흑우 동결 정액으로 체외수정란 생산 효과)

  • Cho, Sang-Rae;Choi, Sun-Ho;Choe, Chang-Yong;Son, Jun-Kyu;Kim, Jae-Bum;Kim, Sung-Jae;Son, Dong-Soo;Kim, Hyun-Jong
    • Journal of Embryo Transfer
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    • v.24 no.3
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    • pp.207-212
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    • 2009
  • The aim of present experiment was to examine commercial synthetic extender(AndroMed) for semen cryopreservation of Korean Black Bull. Semen was collected from a Korean Black Bull using an artificial vagina and transported to the laboratory. The semen was diluted 1:1 by AndroMed. The pellect was diluted to final sperm concentration of $5{\times}10^5/ml$ by doubling in every 10 minutes at $4^{\circ}C$ cold chamber. The semen was equilibrated for 1 hr at cold chamber and packed to 0.5 ml straw. The semen straws were located above 5 cm of liquid nitrogen for 5 minutes, above 5 cm for 10 minutes and above 10 cm for 10 min. And then the frozen straw was plunged to $LN_2$. The presented straws were examined the viability and motility after thawed at $37^{\circ}C$ water bath. Hanwoo semen was used as KPN (Korea Proven Bull Number) in this experiment. The survival rates was significantly higher in fresh semen than frozen semen ($80{\pm}14%\;and\;43{\pm}11%$). However, the motility rates was similar (80.7% and 66.4%). The survival and motility rates were higher in 5cm, 10 min treatment group than the other two groups in straw-located height and duration above $LN_2$ ($50{\pm}14%$ and 70.7% vs, 33.18% and $65{\pm}7%$ vs, 30.14% and 65.7%, respectively). The development rates to cleavage was higher in Black Cow than Hanwoo semen (62.2%, 64.4%), However, The development rates to blastocyst was higher in Hanwoo than Black cow semen (25.9%, 23.0%). In conclusion. The present results that acceptable fertilization and cryopreservation could be obtained by in vitro fertilization with frozen-thawed semen using a synthetic semen extender (AndroMed).

Fish length dependence of target strength for black rockfish, goldeye rockfish at 70kHz and 120kHz (70kHz 및 120kHz에 있어서 조피볼락, 불볼락에 대한 반사강도의 체장 의존성)

  • Mun, Jae-Ho;Lee, Dae-Jae;Shin, Hyeong-Il;Lee, Yoo-Won
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.42 no.1
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    • pp.30-37
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    • 2006
  • Black rockfish and goldeye rockfish are commercially important fish species due to the increasing demand in Korea. When estimating the abundance of stocks for these species acoustically, it is of crucial importance to know the target strength(TS) to length dependence. In relation to these needs, TS measurement was conducted on black rockfish and goldeye rockfish in an acrylic salt water tank using 70kHz and 120kHz split beam echo sounders. The TS for these two species under the controlled condition was simultaneously measured with the swimming movement by DVR system and analyzed as a function of fish length(L). The results obtained are summarized as follows: The best fit regression of TS on fish length of black rockfish was TS=19.38 Log(L, cm)-70.46 ($r^2=0.71$) at 70kHz and TS=22.39 Log(L, cm)-70.40 ($r^2=0.64$) at 120kHz and in the standard form TS=20 Log(L, cm)-71.29 ($r^2 = 0.70$) at 70kHz and TS=20 Log(L, cm)-66.88 ($r^2=0.57$) at 120kHz. The best fit regression of TS on fish length of goldeye rockfish was TS=17.10 Log(L, cm)-68.28 ($r^2=0.37$) at 70kHz and TS=24.39 Log(L, cm)-73.74 ($r^2=0.59$) at 120kHz and in the standard form TS=20 Log(L, cm)-72.03 ($r^2=0.32$) at 70kHz and TS=20 Log(L, cm)-67.68 ($r^2=0.64$) at 120kHz. An empirical model for fish TS(dB) averaged over the dorsal aspect of 115 fishes of black rockfish and goldeye rockfish and which spans the fish length(L, m) to wavelength($\lambda$, m) ratio between 8 and 30 was derived : TS=34.12 Log(L)-14.12 Log($\lambda$)-23.83, ($r^2=0.90$).

Studies on Changes of Isoflavone and Nutrients during Germination of Soybean Varieties (발아기간에 따른 대두 품종별 이소플라본 및 영양성분 변화)

  • Jeon, Su-Hyeon;Lee, Kyong-Ae;Byoun, Kwang-Eui
    • Korean Journal of Human Ecology
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    • v.14 no.3
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    • pp.485-489
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    • 2005
  • The purpose of this study is to investigate changes of isoflavone and nutrients during germination of soybean varieties and to determine the optimal germination condition for soybean processing. After 5 days' germination, water content of the soybeans increased in the order of Poongsannamoolkong> Sinpaldal#2> Black#1> Jinpumkong. Ash content was higher in the order of Poongsannamoolkong> Sinpaldal#2> Jinpumkong> black#1 soybean. All varieties of the soybean samples increased in ash content during germination. Crude fat content was higher in the order of Poongsannamoolkong> Black#1> Jinpumkong> Shinpaldalkong. Crude protein content was higher in the order of Shinpaldalkong> Black#1> Jinpumkong> Poongsannamoolkong. Polypeptide subunits separated from the SDS-PAGE of the entire proteins were showing comparatively high level in the range of 2-16KD in Jinpumkong and 2-21KD in Poongsannamoolkong till the first day of germination, 2-25KD in Sinpaldal#2 till the second day, and 6-27KD in Black#l till the third day. Changes of total isoflavone contents during germination showed different patterns in soybean varieties: Jinpumkong and Black#1 had the highest total isoflavone content on the fourth day of germination, Poongsannamoolkong and Sinpaldal#2 on the first and the second day of germination, respectively.

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Isolation of Anthocyanin from Black Rice (Heugjinjubyeo) and Screening of its Antioxidant Activities (흑미(현진주벼)유래 안토시아닌의 항산화능 탐색)

  • Park, Young-Sam;Kim, Sun-Joong;Chang, Hyo-Ihl
    • Microbiology and Biotechnology Letters
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    • v.36 no.1
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    • pp.55-60
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    • 2008
  • Colored rices are a hulled grains having red or purple pigments in bran. Especially black rice (Heugjinjubyeo) is considered to be a healthy food in Asia. Black rice is of great interesting because of the possible biological activity with their anthocyanins. Anthocyanins are water-soluble plant pigments and representatives of flavonoids. The anthocyanins in black rice include cyanidin 3-O-glucoside, peonidin 3-O-glucoside, malvidin 3-O-glucoside, pelagonidin 3-O-glucoside and delphinidin 3-O-glucoside. In this study, anthocyanins in a black rice were analyzed quantitatively and qualitatively with HPLC and UV-Vis spectrophotometer. The anthocyanins contained approximately 95% of cyanidin-3-O-glucoside and 5% of peonidin-3-O-glucoside. Antioxidant activities of the anthocyanin extract were investigated by using various in vitro methods. The 100g/ml concentration of the anthocyanin extracted exhibited 88.83% inhibition on the peroxidation of linoleic acid, 55.20% DPPH free radical scavenging activity, 54.96% superoxide anion radical scavenging activity, and 72.67% hydrogen peroxide scavenging activity. And it also showed high ferrous ion reducing capability. These results suggest that the anthocyanin extracted from black rice may be utilized as a possible antioxdiant agent against ROS.

Physicochemical Characteristics of Korean Black Cattle-Fed Mugwort (인진쑥 첨가사료를 급여한 흑우육의 이화학적 품질특성)

  • Moon, Yoon-Hee;Jung, In-Chul
    • Journal of Life Science
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    • v.22 no.5
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    • pp.587-594
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    • 2012
  • TMR (total mixed ration) feed was developed by adding mugwort and was fed to Korean black cattle. The effects on the physicochemical properties of the Korean black cattle, when fed mugwort, were investigated, as was the feasibility of producing beef with high quality and functionality. Korean black cattle were reared by using basal TMR (control) and basal TMR supplemented with mugwort of middle fattening 4.6% and late fattening 6.5% (treatment). The content of total catechin in Korean black cattle fed with the control and treatment was 0.262 and 0.379 mg/kg, respectively, while the content of epicatechin was 0.042 and 0.059 mg/kg, respectively, both of which were a significant increase from feeding the cattle TMR with mugwort ($p$ <0.05). There was no significant difference between the control and treatment in terms of $L^*$ (lightness), $b^*$ (yellowness), pH, VBN (volatile basic nitrogen) content, bacterial counts, water-holding capacity, freezing loss, thawing loss, cooking loss, cohesiveness, chewiness, shear force, and sensory score. TBARS (2-thiobarbituric acid reactive substances) and springiness for the control were significantly higher than the treatment ($p$ <0.05). The $a^*$ (redness), EDA (electron donating ability), hardness, and gumminess for the treatment were significantly higher than for the control ($p$ <0.05). These results suggest that the feed containing mugwort can be used to improve color and increase antioxidant ability as functional feed.