Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Effect of Production In Vitro Embryo with Frozen-thawed Semen using AndroMed Extender in Korean Black Cow Semen

AndroMed를 이용한 흑우 동결 정액으로 체외수정란 생산 효과

  • Cho, Sang-Rae (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Choi, Sun-Ho (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Choe, Chang-Yong (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Son, Jun-Kyu (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Jae-Bum (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Sung-Jae (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Son, Dong-Soo (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Hyun-Jong (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
  • 조상래 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 최선호 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 최창용 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 손준규 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김재범 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김성재 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 손동수 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김현종 (농촌진흥청 국립축산과학원 가축유전자원시험장)
  • Published : 2009.09.30
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Abstract

The aim of present experiment was to examine commercial synthetic extender(AndroMed) for semen cryopreservation of Korean Black Bull. Semen was collected from a Korean Black Bull using an artificial vagina and transported to the laboratory. The semen was diluted 1:1 by AndroMed. The pellect was diluted to final sperm concentration of $5{\times}10^5/ml$ by doubling in every 10 minutes at $4^{\circ}C$ cold chamber. The semen was equilibrated for 1 hr at cold chamber and packed to 0.5 ml straw. The semen straws were located above 5 cm of liquid nitrogen for 5 minutes, above 5 cm for 10 minutes and above 10 cm for 10 min. And then the frozen straw was plunged to $LN_2$. The presented straws were examined the viability and motility after thawed at $37^{\circ}C$ water bath. Hanwoo semen was used as KPN (Korea Proven Bull Number) in this experiment. The survival rates was significantly higher in fresh semen than frozen semen ($80{\pm}14%\;and\;43{\pm}11%$). However, the motility rates was similar (80.7% and 66.4%). The survival and motility rates were higher in 5cm, 10 min treatment group than the other two groups in straw-located height and duration above $LN_2$ ($50{\pm}14%$ and 70.7% vs, 33.18% and $65{\pm}7%$ vs, 30.14% and 65.7%, respectively). The development rates to cleavage was higher in Black Cow than Hanwoo semen (62.2%, 64.4%), However, The development rates to blastocyst was higher in Hanwoo than Black cow semen (25.9%, 23.0%). In conclusion. The present results that acceptable fertilization and cryopreservation could be obtained by in vitro fertilization with frozen-thawed semen using a synthetic semen extender (AndroMed).

Keywords

References

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