• Parasites, Hosts and Diseases
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• 제52권4호
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• pp.435-438
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• 2014

The disinfectant effects (DEs) of 10 types of chemicals, defined by their ability to destroy or inhibit oocysts and consequently prevent sporulation of Eimeria tenella field isolate, were evaluated in vitro. Correct species assignments and sample purities were confirmed by the singular internal transcribed spacer (ITS)-PCR analysis. A total of 18 treatments were performed, and the disinfection suppression levels were 75.9% for 39% benzene + 22% xylene (1:10 dilution), 85.5% for 30% cresol soup (1:1 dilution), and 91.7% for 99.9% acetic acid (1:2 dilution) group. The results indicate that acetic acid, cresol soup, and benzene+xylene are good candidates for suppression of E. tenella oocyst sporulation.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2003년도 추계학술대회
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• pp.109-110
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• 2003

To validate a novel mouse model, gpt delta, for in vivo mutagenesis, the Mammalian Mutagenesis Society (MMS), a subgroup of the Environmental Mutagen Society of Japan (JEMS) (JEMS/MMS), performed a collaborative study as the third trial for transgenic animal assay. In this mouse model, point mutations and deletions re separately identified by gpt (6-thioguanine-resistant) and Spi- (sensitive to P2 interference) selections, respectively.(omitted)

• Journal of Ginseng Research
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• 제48권3호
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• pp.245-252
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• 2024

Ginseng is a traditional herbal medicine used for prevention and treatment of various diseases as a tonic. Recent scientific cohort studies on life prolongation with ginseng consumption support this record, as those who consumed ginseng for more than 5 years had reduced mortality and cognitive decline compared to those who did not. Clinical studies have also shown that acute or long-term intake of ginseng total extract improves acute working memory performance or cognitive function in healthy individuals and those with subjective memory impairment (SMI), mild cognitive impairment (MCI), or early Alzheimer's disease (AD) dementia who are taking AD medication(s). Ginseng contains various components ranging from classical ginsenosides and polysaccharides to more recently described gintonin. However, it is unclear which ginseng component(s) might be the main candidate that contribute to memory or cognitive improvements or prevent cognitive decline in older individuals. This review describes recent clinical contributors to ginseng components in clinical tests and introduces emerging evidence that ginseng components could be novel candidates for cognitive improvement in older individuals, as ginseng components improve SMI cognition and exhibits add-on effects when coadministered with early AD dementia drugs. The mechanism behind the beneficial effects of ginseng components and how it improves cognition are presented. Additionally, this review shows how ginseng components can contribute to SMI, MCI, or early AD dementia when used as a supplementary food and/or medicine, and proposes a novel combination therapy of current AD medicines with ginseng component(s).

• 환경생물
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• 제18권2호
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• pp.205-213
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• 2000

실험실에서 형질전환될 수 있는 세균들은 자연환경 조건에서도 형질전환 능력이 발달하는 것으로 알려져 있다. 따라서, 환경 내에서 형질전환 능력이 있는 세균의 존재는 확실한 것으로 여겨진다. DNA는 무기물에 부착된 상태에서는 핵산분해효소에 의한 분해로부터 보호되는 것으로 알려져 있다. 비록 DNA가 토양 속에 분산되어져 일정 비율로 가수분해되더라도, 수 주일 후에도 낮은 비율로 감지될 수 있다. 따라서 free DNA는 자연적 형질전환을 할 수 있을 만큼 충분히 지속될 수 있다. 실험실 조건에서는 세균의 형질전환이 여러 경우 보고되었지만, 자연상태에서 형질전환과 관련된 자료는 매우 적다. 생태학적으로 GMMs로부터 재조합 DNA가 토착 미생물에 전이될 수 있는 잠재력에 대한 문제가 주요 현안이 되었는데, 이는 전이된 DNA가 방출된 세균의 생태학적인 적응력을 변화시켜 생물학적 안전성의 문제를 야기할 수 있기 때문이다. 물론, 방출된 GMMs로부터 재조합 DNA가 토착 미생물에 전이되는 율은 아주 낮은 빈도로 일어나지만, 빈도가 낮다는 것은 그리 중요하지 않다. 왜냐하면, 비록 낮은 빈도로 전이되더라도 유리한 조건을 만나게 되면 전이된 유전자는 선택될 수 있기 때문이다. 이제까지 GMMs는 실험실이나 제한된 환경에서 주로 사용되었지만 앞으로는 개방된 자연 생태계에서 이루어질 전망이다. 그러므로 GMMs가 토착세균에 미치는 영향에 대해서도 연구되어야 하고 동시에 GMMs가 생태계에 방출될 경우 그에 따른 영향평가를 반드시 수행해야 한다.

• Journal of Ginseng Research
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• 제48권1호
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• pp.1-11
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• 2024

Fresh ginseng is prone to spoilage due to its high moisture content. For long-term storage, most fresh ginsengs are dried to white ginseng (WG) or steamed for hours at high temperature/pressure and dried to form Korean Red ginseng (KRG). They are further processed for ginseng products when subjected to hot water extraction/concentration under pressure. These WG or KRG preparation processes affect ginsenoside compositions and also other ginseng components, probably during treatments like steaming and drying, to form diverse bioactive phospholipids. It is known that ginseng contains high amounts of gintonin lysophosphatidic acids (LPAs). LPAs are simple lipid-derived growth factors in animals and humans and act as exogenous ligands of six GTP-binding-protein coupled LPA receptor subtypes. LPAs play diverse roles ranging from brain development to hair growth in animals and humans. LPA-mediated signaling pathways involve various GTP-binding proteins to regulate downstream pathways like [Ca²⁺]_i transient induction. Recent studies have shown that gintonin exhibits anti-Alzheimer's disease and antiarthritis effects in vitro and in vivo mediated by gintonin LPAs, the active ingredients of gintonin, a ginseng-derived neurotrophin. However, little is known about how gintonin LPAs are formed in high amounts in ginseng compared to other herbs. This review introduces atypical or non-enzymatic pathways under the conversion of ginseng phospholipids into gintonin LPAs during steaming and extraction/concentration processes, which exert beneficial effects against degenerative diseases, including Alzheimer's disease and arthritis in animals and humans via LPA receptors.

• 한국산업보건학회지
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• 제20권1호
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• pp.41-46
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• 2010

The purpose of this study is to assess the level of fungi concentration in the university laboratories in Seoul, Korea, and to investigate factors contributing to these concentrations. The samples were taken from three spots in each laboratory; the top of sink, the center of laboratory, and the front of ventilation system, i.e fume hood at the chemical laboratory and clean bench/biosafety cabinet at the microbial laboratory. Air samples were collected using the single-stage Anderson sampler (Quick Take 30) at a flow rate of 28.3 l/min for 5 min on nutrient media in Petri-dishes located on the impactor. Fifty-two air samples were collected from 19 different laboratories (13 microbiology laboratories, 6 chemistry laboratories) in the university, and concentrations of airborne fungi showed no significant difference (p>0.05) between microbiology and chemistry laboratory, and also no significant difference at three locations (sink, center, front of ventilation system) in microbiology and chemistry laboratories. Average concentrations of fungi in 19 laboratories ranged from 7 to 459 cfu/$m^3$, with an overall Geometric Mean of 52 cfu/$m^3$. Airborne fungi concentrations of 6 samples (12 %) exceeded 150 cfu/$m^3$, the guideline of WHO. The ratios of Indoor/Outdoor for airborne fungi ranged from 0.2 to 4.8 (mean = 1.6). Related factors were measured such as relative humidity, temperature, and laboratory area. Temperature and laboratory area showed no significant relations to concentrations of airborne fungi except for relative humidity in the laboratory Concentrations of fungi were significant different (p<0.01) between rainy or cloudy and sunny. However, there was no significant difference between general ventilation and nongeneral ventilation.

• Molecules and Cells
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• 제45권12호
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• pp.896-910
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• 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly transmissible and potentially fatal virus. So far, most comprehensive analyses encompassing clinical and transcriptional manifestation have concentrated on the lungs. Here, we confirmed evident signs of viral infection in the lungs and spleen of SARS-CoV-2-infected K18-hACE2 mice, which replicate the phenotype and infection symptoms in hospitalized humans. Seven days post viral detection in organs, infected mice showed decreased vital signs, leading to death. Bronchopneumonia due to infiltration of leukocytes in the lungs and reduction in the spleen lymphocyte region were observed. Transcriptome profiling implicated the meticulous regulation of distress and recovery from cytokine-mediated immunity by distinct immune cell types in a time-dependent manner. In lungs, the chemokine-driven response to viral invasion was highly elevated at 2 days post infection (dpi). In late infection, diseased lungs, post the innate immune process, showed recovery signs. The spleen established an even more immediate line of defense than the lungs, and the cytokine expression profile dropped at 7 dpi. At 5 dpi, spleen samples diverged into two distinct groups with different transcriptome profile and pathophysiology. Inhibition of consecutive host cell viral entry and massive immunoglobulin production and proteolysis inhibition seemed that one group endeavored to survive, while the other group struggled with developmental regeneration against consistent viral intrusion through the replication cycle. Our results may contribute to improved understanding of the longitudinal response to viral infection and development of potential therapeutics for hospitalized patients affected by SARS-CoV-2.

• IMMUNE NETWORK
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• 제24권2호
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• pp.7.1-7.19
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• 2024

Viral load and the duration of viral shedding of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are important determinants of the transmission of coronavirus disease 2019. In this study, we examined the effects of viral doses on the lung and spleen of K18-hACE2 transgenic mice by temporal histological and transcriptional analyses. Approximately, 1×10⁵ plaque-forming units (PFU) of SARS-CoV-2 induced strong host responses in the lungs from 2 days post inoculation (dpi) which did not recover until the mice died, whereas responses to the virus were obvious at 5 days, recovering to the basal state by 14 dpi at 1×10² PFU. Further, flow cytometry showed that number of CD8+ T cells continuously increased in 1×10² PFU-virus-infected lungs from 2 dpi, but not in 1×10⁵ PFU-virus-infected lungs. In spleens, responses to the virus were prominent from 2 dpi, and number of B cells was significantly decreased at 1×10⁵ PFU; however, 1×1² PFU of virus induced very weak responses from 2 dpi which recovered by 10 dpi. Although the defense responses returned to normal and the mice survived, lung histology showed evidence of fibrosis, suggesting sequelae of SARS-CoV-2 infection. Our findings indicate that specific effectors of the immune response in the lung and spleen were either increased or depleted in response to doses of SARS-CoV-2. This study demonstrated that the response of local and systemic immune effectors to a viral infection varies with viral dose, which either exacerbates the severity of the infection or accelerates its elimination.

• Toxicological Research
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• 제30권2호
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• pp.71-75
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• 2014

Ambient air pollution (AAP) and particulate matters (PM) have been closely associated with adverse health effects such as respiratory disease and cardiovascular diseases. Previous studies have examined the adverse health effects associated with short- and long-term exposure to AAP and outdoor PM on respiratory disease. However, the effect of PM size ($PM_{2.5}$ and $PM_{10}$) on cardiovascular disease has not been well studied. Thus, it remains unclear how the size of the inhalable particles (coarse, fine, or ultrafine) affects mortality and morbidity. Airborne PM concentrations are commonly used for ambient air quality management worldwide, owing to the known effects on cardiorespiratory health. In this article, we assess the relationship between cardiovascular diseases and PM, with a particular focus on PM size. We discuss the association of $PM_{2.5}$ and $PM_{10}$, nitrogen dioxide ($NO_2$), and elemental carbon with mortality and morbidity due to cardiovascular diseases, stroke, and altered blood pressure, based on epidemiological studies. In addition, we provide evidence that the adverse health effects of AAP and PM are more pronounced among the elderly, children, and people with preexisting cardiovascular and respiratory conditions. Finally, we critically summarize the literature pertaining to cardiovascular diseases, including atherosclerosis and stroke, and introduce potential studies to better understand the health significance of AAP and PM on cardiovascular disease.

• Journal of Microbiology and Biotechnology
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• 제26권1호
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• pp.130-138
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• 2016

The use of antibiotics in aquaculture raises environmental and food safety concerns because chronic exposure of an aquatic ecosystem to antibiotics can result in the spread of antibiotic resistance, bioaccumulation of antibiotics in the organisms, and transfer of antibiotics to humans. In an attempt to overcome these problems, high-concentration red clay was applied as an alternative antibiotic against the following common fish pathogens: Aeromonas salmonicida, Vibrio alginolyticus, and Streptococcus equinus. The growth of A. salmonicida and V. alginolyticus was retarded by red clay, whereas that of S. equinus was promoted. Phase contrast and scanning electron microscopy analyses confirmed the attachment of red clay on cell surfaces, resulting in rapid gravitational removal and cell surface damage in both A. salmonicida and V. alginolyticus, but not in S. equinus. Different cell wall properties of grampositive species may explain the unharmed cell surface of S. equinus. Significant levels of oxidative stress were generated in only the former two species, whereas significant changes in membrane permeability were found only in S. equinus, probably because of its physiological adaptation. The bacterial communities in water samples from Oncorhynchus mykiss aquacultures supplemented with red clay showed similar structure and diversity as those from oxytetracycline-treated water. Taken together, the antibiotic effects of high concentrations of red clay in aquaculture can be attributed to gravitational removal, cell surface damage, and oxidative stress production, and suggest that red clay may be used as an alternative for antibiotics in aquaculture.