• KSBB Journal
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• 제29권1호
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• pp.42-49
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• 2014

The aim of this study was to develop a composite human skin equivalent for wound healing. Collagen type1 and acellular dermal matrix powder were utilized as the scaffold with dermal fibroblasts and keratinocytes for the development of a composite human skin equivalent. Fibroblast maintained the volume of composite skin equivalent and also induced keratinocytes to attach and proliferate on the surface of composite skin equivalent. The composite human skin equivalent had a structure and curvature similar to those of real skin. Balb-C nu/nu mice were used for the evaluation of full-thickness wound healing effect of the composite human skin equivalent. Graft of composite skin equivalent on full-thickness wound promoted re-epithelialization and granulation tissue formation at 9 days. Given the average wound-healing time (14 days), the wound in the developed composite skin equivalent healed quickly. The overall results indicated that this three-dimensional composite human skin equivalent can be used to effectively enhance wound healing.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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• pp.529-543
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• 2003

Real-time measurements of skin movement induced by air blown on the surface was measured with time. We investigated age-related changes in displacement of the skin surface on the face or the inner upper arm caused by air on 98 Japanese women volunteers aged from 10 to 70 years old. The maximum distance (the denting state) that the skin moved reached 2-5 mm within 10-15 msec on the cheek skin. After that, the skin generally recovered to the original state within 40-50 msec. The maximum speed of movement was 0.5 m/sec and the recovery speed was about 0.25 m/sec on the cheek skin. Significant changes with age were not observed in the denting state, but a significant correlation with age was observed in the recovery state. For example, the maximum recovery speed decreased significantly with age (p=-0.568, p<0.001) and the time required for recovery increased significantly with age (p=0.561, p<0.001). Although the inner upper arm also showed similar results to a cheek, a few parameters were different. This apparatus is a more practical macroscopic system for evaluating skin mechanical properties without contact. This apparatus is effective not only for measuring the mechanical properties of facial skin but also of body skin, such as swelling or sagging of body parts.

• Fisheries and Aquatic Sciences
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• 제21권4호
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• pp.7.1-7.8
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• 2018

In this study, we isolated and characterized the acid-soluble skin collagen of Pacific bluefin tuna (PBT, Thunnus orientalis). The PBT skin collagen was composed of two ${\alpha}$ chains (${\alpha}1$ and ${\alpha}2$) and one ${\beta}$ chain. The denaturation temperature of PBT collagen was low although it was rich in proline and hydroxyproline. The primary structure of PBT skin collagen was almost identical to that of calf and salmon skin collagen; however, it differed with respect to the epitope recognition of the antibody against salmon type I collagen. These results suggest that the primary structure of skin collagen was highly conserved among animal species, although partial sequences that included the epitope structure differed among collagens.

• Journal of Microbiology and Biotechnology
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• 제27권9호
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• pp.1657-1663
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• 2017

Cosmetic patches have recently been developed as skin products for personal care owing to rapid advances in the technology of delivery of active ingredients, moisture, and adhesiveness to skin. Alginate and Spirulina are typical marine resources used in cosmetic products. This research involved the development of a Spirulina extract-impregnated alginate nanofiber cosmetic patch supported by a polycaprolactone (PCL) nanofiber cover (Spi/Alg-PCL NF patch). In addition to the ability of alginate to affect moisture and adhesiveness to skin, the impregnation of Spirulina extract strengthened those abilities as well as its own bioactive effectiveness. All fabrication processing steps were undertaken in aqueous solution. The three components (alginate, Spirulina extract, and PCL) had no detected cytotoxicity in human keratinocyte cell-based examination. In addition, wetting the pre-dried patch on the skin resulted in the Spirulina extract being released within 30 min. The results indicate the excellence of the Spi/Alg-PCL NF patch as a skin-care cosmetic device.

• 대한의생명과학회지
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• 제17권1호
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• pp.47-53
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• 2011

This study is aimed to investigate the skin thickness, plasma components, serum IgE level and antioxidant enzymes activities by mixtures of Cnidium officinale, Saururus chinensis, Houttuynia cordata and Glycyrrhiza uralensis on the allergic contact dermatitis of rat induced by 1-chloro-2,4-dinitrochlorobenzene (DNCB). Mixtures reduced the thickness of the skin and removed the dead skin cells compared to the skin of rats treated with DNCB alone. Also, these mixtures down-regulated the contents of lipid and IgE, and reduced the activities of superoxide dismutase and catalase. These results indicate that the mixtures significantly recovered the contact dermatitis induced by DNCB. In conclusion, it is thought that the mixtures could be useful for the allergic contact dermatitis.

• Fisheries and Aquatic Sciences
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• 제22권5호
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• pp.10.1-10.14
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• 2019

Fish skin waste accounts for part of the solid waste generated from seafood processing. Utilization of fish skin by bioconversion into high-grade products would potentially reduce pollution and economic cost associated with treating fish processing waste. Fish skin is an abundant supply of gelatin and collagen which can be hydrolyzed to produce bioactive peptides of 2-20 amino acid sequences. Bioactivity of peptides purified from fish skin includes a range of activities such as antihypertensive, anti-oxidative, antimicrobial, neuroprotection, antihyperglycemic, and anti-aging. Fish skin acts as a physical barrier and chemical barrier through antimicrobial peptide innate immune action and other functional peptides. Small peptides have been demonstrated to possess biological activities which are based on their amino acid composition and sequence. Fish skin-derived peptides contain a high content of hydrophobic amino acids which contribute to the antioxidant and angiotensin-converting enzyme inhibitory activity. The peptide-specific composition and sequence discussed in this review can be potentially utilized in the development of pharmaceutical and nutraceutical products.

• Journal of Korean Biological Nursing Science
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• 제17권2호
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• pp.132-139
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• 2015

Purpose: The purpose of this study was to verify the correlation analysis between fluid intake on skin hydration and pH of college students. Methods: The subjects were 129 female nursing students in D city. Data were collected by a self-administered questionnaire, using a skin moisture checker and skin pH meter on faces, hands, and feet. The collected data were analyzed by descriptive statistics, t-test, ANOVA, and Pearson correlation analysis, using SPSS WIN, 20. Results: The daily fluid intake was composed of 57% pure water, 21% caffeinated beverages, 22% non-caffeinated beverages. There were significant differences in average skin hydration on the three body parts according to pure water, caffeinated beverages, and non-caffeinated beverages; however, there was no significant difference measured by fluid intake. There was a significant positive correlation between fluid intake and skin hydration: between pure water and skin hydration. There was significant negative correlation between caffeinated beverages and skin hydration: between non-caffeinated beverages and skin hydration. Conclusion: The results suggest that fluid intake, pure water, caffeinated beverages, and non-caffeinated beverages have an effect on skin hydration and pH. Therefore, it is good to increase the amount of fluid intake but, it is recommended to increase the amount of intake of pure water rather than beverages to improve skin status.

• 한국식생활문화학회지
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• 제34권5호
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• pp.645-650
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• 2019

Skin plays important roles in protecting the internal organs from the chemical-biological risk factors and ultraviolet light. Exposure to the chemical and biological stimuli has a detrimental effect on skin's structure and physiological regulation. Therefore, much attention has been paid to natural products that show biological activities such as anti-oxidation, anti-aging and anti-bacterial activities. In this study, we investigated the skin-related biological activities of Oenothera lamarckiana aerial part extract. The extract contained 229.35 mg TAE (tannic acid equivalents)/g total polyphenolic compounds and the extract showed relative high antioxidant activity ($SC_{50}$ value: $8.52{\mu}g/mL$). The $IC_{50}$ value against tyrosinase and elastase were 307.94 and $181.51{\mu}g/mL$, respectively. This suggested that O. lamarckiana can be applied to whiten skin and slow the aging of skin. O. lamarckiana extract showed a growth inhibitory effect on Staphylococcu epidermidis (minimum inhibitory concentration: $250{\mu}g/mL$). Interestingly, O. lamarckiana extract showed no inhibitory effect on Pseudomonas aeruginosa on the paper disc assay. Yet the extract inhibited the growth of Pseudomonas aeruginosa on the broth dilution assay in a dose-dependent manners. Taken together, O. lamarckiana could have good potential for development as an additive in the cosmetic industry.

• Animal cells and systems
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• 제4권4호
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• pp.315-318
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• 2000

Structure of the skin in amphibious fish, Periophthalmus modestus, was described in relation to cutaneous respiration. The epidermis has no gland cell. The epidermis consists of three regions: outermost layer of one to five layers of flattened epithelial cells, middle layer of swollen epithelial cells instead of glandular cells and stratum germinativum of cuboidal cells. There are numerous blood capillaries in the outermost layer of the epidermis and diffusion distance between the blood of capillaries and the epidermis is about 1.4 Um. The middle layer of the epidermis appears to be a web-like structure due to the swollen epithelial cells. The stratum germinativum has a well-developed lymphatic space containing lymphocytes. There are numerous blood capillaries and elliptical area with acid mucopolysaccharides in stratum laxum of the dermis. The skin of Periophthalmus modestus may be an accessory respiratory organ for oxygen uptake during terrestrial or aquatic life.

• Animal cells and systems
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• 제5권4호
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• pp.303-308
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• 2001

The skin structure of Misgurnus mizolepis was studied based on the microanatomical investigation of skin fragments taken from four regions. The epidermis was distinguished by two types of skin glands, a small mucous cell and a large club cell. The mucous cell was acid sulfomucins (some sialomucins) but the club cell did not give any histochemical tests for mucosubstances. The presence of a well defined lymphatic system with small lymphocytes was established in the stratum germinativum layer of the epidermis. A large number of blood capillaries run very close to each other just below the basement membrane, and a definite area giving AB and PAS positive was present between the basement membrane and scale. These structural features of skin in M. mizolepis seem to be closely related with cutaneous respiration.