• Journal of the Society of Cosmetic Scientists of Korea
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• v.42 no.3
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• pp.211-216
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• 2016

Micronucleus test is genotoxicity assay for detection of micronuclei in the cytoplasm of interphase cells. The reduction and replacement of in vivo toxicity testing on animals require the development of in vitro models to predict the genotoxicity or other tests for cosmetic products. In this study, we evaluated a genotoxicity assay for topically applied chemicals using a three-dimensional human reconstructed skin model, KeraSkin$^{TM}$. Two genotoxins, mitomycin C (MMC) and methyl methanesulfonate (MMS), induced significant dose-related increases in cytotoxicity and micronuclei induction in the skin model. In contrast, two non-genotoxins, 4-nitrophenol (4-NP) and trichloroethylene (TCE), induced cytotoxicity but not micronucleus formation. In conclusion, micronucleus test using human skin model may be useful for predicting in vitro genotoxic potentials of cosmetic products.

• Journal of Digital Convergence
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• v.13 no.5
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• pp.337-344
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• 2015

Medicinal plants containing wellness-fusion-complex compound are increasingly being pursued as suitable alternative sources of various biological properties. In this study, inhibitory effect of Quintinia acutifolia, which is a New Zealand plant, on P388 murine lymphocytic leukemia cells using MTT [3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide] assay. Based on $^1H-NMR$, $^{13}C-NMR$ spectral data and other spectral analysis, 2,3,2'',3''-tetrahydroochanaflavone (1) and 2'',3''-dihydroochana-flavone (3) inhibited the leukemia cells were purified from the plants. 2,3,2'',3''-tetrahydroochanaflavone (1) and 2'',3''-dihydroochana-flavone (3) are biflavonoids possessing two basic flavonoids and actively inhibited growth of P388 murine lymphocytic leukemia cells with a 50% inhibitory concentration ($IC_{50}$) of $8.2{\mu}g/mL$ and $3.1{\mu}g/mL$, respectively. Specially, 2'',3''-dihydroochana-flavone (3) possessed unconjugated flavonone system, which isn't consist of a pair with B ring of 2,3,2'',3''-tetrahydroochanaflavone (1). Therefore, the two compounds could be considered as a candidate for development of anticancer drugs and need to much studies in the future.

• Korean Journal of Ichthyology
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• v.29 no.2
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• pp.146-150
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• 2017

Length and weight relationship (LWR) for dominant Antarctic fishes was determined in two species of the family Nototheniidae; black rockcod (Notothenia coriiceps) and marbled rockcod (Notothenia rossii). Samples were caught in the offshore sea around King Sejong station located on King George Island, Antarctica in January, 2017. A total of 30 N. coriiceps and 7 N. rossii were caught by fishing rod and hook. Average total length was 266.0 mm for N. coriiceps and 275.4 mm for N. rossii. Average total weight was 283.1g for N. coriiceps and 290 g for N. rossii. In terms of LWR and b value, the results showed that both two species had positive allometries (b>3) in good health. This size information of two dominant Antarctic fishes would be useful for future physiological studies to understand of adaptation mechanism and biological pathway of Antarctic marine organisms.

• Journal of Science and Technology Studies
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• v.12 no.1
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• pp.1-43
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• 2012

This paper examines the American Civil Liberties Union(ACLU)'s recent legal challenge on patents held by Myriad Genetics on two genes (BRCA1 and BRCA2) associated with a high risk of breast and ovarian cancer. Instead of analyzing the ACLU's objections to the BRCA patents in terms of its legal technicalities and normative ethical principles, this paper seeks to situate this legal case in the broader historical context of the shifting understanding of the relationship between private ownership, economic development, and the public interest in academic sciences. This paper first briefly chronicles a series of scientific developments and key legal decisions involving patenting of life forms, including genetically engineered micro-organisms animals and biological materials of human origins like cell cultures and genes, that led to the US Patent and Trademark Office(USPTO)'s official guidelines on human gene patenting in 2001. At another level, this paper analyzes the expansion of the scope of intellectual property rights in the life sciences in terms of shifting economic and legal assumptions about public knowledge and its role for economic development in the 1970s. I then show how these economic, legal, and ethical ideas that linked private ownership and the public interest have been challenged from the 1990s, calling for revisions in intellectual property laws regarding a wide array of life forms. The tragedy of the anticommons in human gene patenting, according to ACLU, has severely undermined creative scientific activities, medical innovations, access to health care and rights to life among cancer patient groups. ACLU's objection to human gene patenting on several US-constitutional grounds in turn suggests issues regarding intellectual property are critically linked to vital issues pertinent to the creative communities in arts and sciences, such as free exchange of ideas, censorship and monopoly, and free expression and piracy etc.

• Korean Journal of Plant Resources
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• v.28 no.4
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• pp.391-399
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• 2015

There has been little research on the harvesting time-dependent changes in the antioxidant activities of new varieties of highland cultures of Gomchwi (Ligularia fischeri) crossed with Turcs (Ligularia fischeri (Ledeb.)) and Nakai (Ligularia fischeri var. spiciformis), namely Sammany (S), Gommany (G) and Damogy (D). This study was conducted to assess the effect of different harvesting times on nutritional and health-related properties such as total phenolic contents, flavonoids, DPPH (1,1-diphenyl-2-picrylhydrasyl) free radical scavenging activities and reducing power. From these harvests, extracts were prepared using methanol. Total phenolic content in Jul 14-G (Gommany harvested on July 14, 0.172 ㎎·GAE/ml) was higher than that in other products harvested after the same period (S, 0.154; D, 0.141 ㎎·GAE/ml). Flavonoid content was higher in Jul 3-G (0.114 ㎎·QE/ml), compared to Jul 3-S (0.113 ㎎·QE/ml) and Jul 14-D (0.089 ㎎·QE/ml). Antioxidant activities were higher in samples harvested after June 12 in all cases. On July 14, the highest DPPH free radical scavenging activities among all harvest dates were seen (92.875~94.595%). The reducing power was also dependent on harvest day (Abs 0.5~0.6 on July 14), showing a pattern similar to that of DPPH free radical-scavenging activities. Antioxidant activity and harvesting times seem to correlate with total polyphenol and flavonoid contents.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.36 no.3B
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• pp.275-286
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• 2011

As the 21st century paradigm of healthcare service changes from post-therapeutic treatment to disease prevention and management in advance, the M2M-based u-healthcare application service is increasingly important. M2M-based u-healthcare application service uses mobile handsets equipped with sensors to measure vital information, and the medical staff in remote locations can manage the health of the patient or the public in real time. In this paper, IEEE/HL7 translation gateway, utilizing the gateway based on M2M u-healthcare application service structure, which is based on international standards, has been designed and implemented. Specifically, the gateway between ISO/IEEE 11073 standards and ANSI HL7 has been developed. The former defines the protocol for the exchange of information between the agent device and the manger devices for measuring and handling biological signal, and the latter defines the application layer protocol for the exchange of various healthcare information systems. Finally, in order to demonstrate the functionality of the proposed architecture, the M2M-based U-healthcare application service based on IEEE/HL7 translation gateway, utilizing the gateway, has been developed which can measure, collect and process a variety of vital signs such as ECG or SpO2.

• Journal of Biomedical Engineering Research
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• v.25 no.6
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• pp.551-556
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• 2004

The purpose of this study was to suggest a systematic and scientific method for measurement of children's growth development, in which the accuracy of existing diagnosis method has not been concretely examined yet. The most popular method for diagnosis of children's growth is to analyze the opening degree of growth plate in each joint by X-ray image. However, X-ray method has some disadvantages; it is impossible to measure the diagnosis of growth periodically and repeatedly due to the radiation problem. Hence, this study introduced a profile analysis and the algorithm of analyzing the image of growth plate with the BUA(Broadband Ultrasound Attenuation) of calcaneus, to verify the possibility of alternative ultrasonic method harmless to human body. We obtained the images of growth plate in proximal tibiae, phalanges, and calcanei of 269 children (7∼16 years old) with X-ray. And the image of growth plate in calcanei was also obtained front those children with ultrasound. The results showed that the time of the opening degree of growth plate in each joint was almost consistent between X-ray and ultrasonic images. Also, the images of growth plate measured by X-ray and ultrasound showed the high correlation. Therefore, it is expected that the algorithm of ultrasonic profile analysis introduced in this study can replace the existing X-ray method to measure the growth plate correctly.

• Journal of Biomedical Engineering Research
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• v.19 no.4
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• pp.403-416
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• 1998

Socket pressure distributions with gait analysis of a trnsfemoral and a transtibial prostheses were measured in order to assess an optimal socket fitting and function. Ankle joint was aligned by the neutral and the dorsi/plantar flexed positions. Compared to dorsi and plantar flexed positions of ankle joint, cadence and walking speed increased with the neutral ankle joint alignment. Other gait parameters were close to the normative data with the neutral ankle joint alignment. For the transfemoral amputee, dorsiflexed alignment of the ankle joint created high pressure on the lateral aspect of the socket, on the other hand, plantarflexed alignment resulted in increased pressure on the medial aspect of the socket. For the transtibial amputee, dorsiflexed alignment of the ankle resulted in high pressure on the antero-lateral aspect of the socket during mid-stance, but apltarflexion of the ankle joint showed slight increases in pressure at the same location in the socket. The present study clearly demonstarted that malalignment of a prosthesis results in localized increasesing pressure within the socket. Proper slignment of the prosthesis is required in order to acquire an appropriate socket-limb interface as well as the proper gait.

• Applied Microscopy
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• v.30 no.1
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• pp.1-9
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• 2000

The wandering spider, Pardosa astrigera, had four pairs of ocelli that arranged in three rows on the cephalothorax. Along the anterior margin lay a pair of small anterior median (AM) eye flanked on each side by anterior lateral (AL) eye. Two large posterior median (PM) eye was situated on the clypeus behind the anterior row and still more posteriorly was a pair of posterior lateral (PL) eye. The visual cell of retina consisted of cell body, rhabdome, and intermediate segment. Bipolar neuron was found in anterior median eye (principal eye) and unipolar neuron in others (secondary eye). Rhabdome showed that arranged in PMeye and PLeye. But rhabdomes of AMeye and ALeye were irregular in retina. Except AMeye, incontinuous tapetum found in ALeye, PMeye, PLeye. Anterior median eye was similar to anterior lateral eye in length and posterior median eye similar to posterior lateral eye. Component size of eye were similar to 4 pairs eye in cornea. Size of lens, cell body, and rhabdome was similar not only anterior median eye and anterior lateral eye but also posterior median eye and posterior lateral eye. Vitreous body was large posterior median eye than others.

• Applied Microscopy
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• v.29 no.2
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• pp.189-193
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• 1999

The response of ameloblast to long term (3 weeks) exposure to fluoride was examined in continuously erupting mandibular incisors of pregnancy rats as compared to control rats receiving a similar diet (Teklad L-356) but no sodium fluoride in there drinking water. Rats were started on water containing 0 ppm, 100 ppm, 200 ppm, and 300 ppm NaF at the beginning of pregnancy. To examine on the ultrastructural changes of the ameloblast, electron microscopy was used. The results indicated that rat incisors expressed two major changes in normal amelogenesis that could be attributed to chronic fluoride treatment. The fluoride produces marked alteration in the fine structure of ameloblast from teeth of young rats, such as large confluent distensions of the endoplasmic reticulum and swelling of isolated mitochondria, in particular on the morphology of the rough-surfaced endoplasmic reticulum. A graded series of alterations to these organelles were produced, and the severity of the changes would seem to be dependent on dose and time. This experimental data suggested that exposure prolonged of animal to high level of fluoride appears to induce morphological changes in the normal appositional growth and initial mineralization of enamel created during amelogenesis.