Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Evaluating the Micronucleus Induction Potential for the Genotoxicity Assay Using the Human Skin Model, KeraSkinTM

인공피부모델 KeraSkinTM을 이용한 유전독성 평가

  • Lee, Su-Hyon (R&D Institute, Biosolution Co., Ltd.) ;
  • Jung, Haeng-Sun (R&D Institute, Biosolution Co., Ltd.) ;
  • Kim, Seol-Yeong (R&D Institute, Biosolution Co., Ltd.) ;
  • Kim, Hye Soo (R&D Institute, Biosolution Co., Ltd.) ;
  • Lim, Kyung-Min (Department of Pharmacy, College of Pharmacy, Ewha Womans University) ;
  • Chung, Young-Shin (Department of Biotechnology, College of Life and Health Sciences, Hoseo University) ;
  • Choe, Tae-Boo (Department of Biological Engineering, College of Engineering, Konkuk University)
  • 이수현 ((주)바이오솔루션 부설연구소) ;
  • 정행선 ((주)바이오솔루션 부설연구소) ;
  • 김설영 ((주)바이오솔루션 부설연구소) ;
  • 김혜수 ((주)바이오솔루션 부설연구소) ;
  • 임경민 (이화여자대학교 약학대학 약학과) ;
  • 정영신 (호서대학교 생명보건과학대학 생명공학과) ;
  • 최태부 (건국대학교 공과대학 생물공학과)
  • Received : 2016.06.15
  • Accepted : 2016.09.13
  • Published : 2016.09.30
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Abstract

Micronucleus test is genotoxicity assay for detection of micronuclei in the cytoplasm of interphase cells. The reduction and replacement of in vivo toxicity testing on animals require the development of in vitro models to predict the genotoxicity or other tests for cosmetic products. In this study, we evaluated a genotoxicity assay for topically applied chemicals using a three-dimensional human reconstructed skin model, KeraSkin$^{TM}$. Two genotoxins, mitomycin C (MMC) and methyl methanesulfonate (MMS), induced significant dose-related increases in cytotoxicity and micronuclei induction in the skin model. In contrast, two non-genotoxins, 4-nitrophenol (4-NP) and trichloroethylene (TCE), induced cytotoxicity but not micronucleus formation. In conclusion, micronucleus test using human skin model may be useful for predicting in vitro genotoxic potentials of cosmetic products.

소핵시험은 세포분열 단계 중 간기 세포의 세포질 내 소핵 유무를 조사함으로써 유전독성을 평가하는 시험법이다. 최근 화장품 안전성 평가에 동물실험을 금지하거나 최소화하려는 노력이 확산되고 있어 유전독성 평가에 있어서도 기존의 동물실험이 아닌 새로운 in vitro 시험법이 요구되고 있다. 본 연구에서는 3차원 배양인공피부모델인 KeraSkin$^{TM}$을 이용하여 도포 처치된 물질의 유전독성을 평가하였다. 2종의 유전독성물질인 mitomycin C (MMC)와 methyl methanesulfonate (MMS)는 농도 의존적으로 세포독성과 소핵 형성이 유도된 반면, 대조물질인 4-nitrophenol (4-NP)와 trichloroethylene (TCE)에서는 농도 의존적으로 세포독성은 관찰되었으나 소핵은 형성되지 않았다. 따라서 인공피부모델을 이용한 소핵시험이 화장품과 같은 피부적용물질의 in vitro 유전독성 평가에 유용할 것으로 사료된다.

Keywords

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