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Chitosan and Acetylchitosan from Squid Pen and Their Characteristics (오징어 연골을 이용한 Chitosan 및 Acetylchitosan의 제조와 특성)

  • CHOI Hyeon-Mee;HWANG Sun-Young;PARK Seong-Min;LEE Keun-Tai
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.5
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    • pp.563-569
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    • 2001
  • In order to utilize the processing wastes of squid, chitosan was prepared by intermittent deacetylation treaoent of $\beta-chitin$ contained richly in the pen of squid. Acetylchitosan also was synthesized from squid pen chitosan with anhydrous acetic acid and their characteristics were investigated. The amounts of nitrogen and ash of squid pen chitosan were $5.80.2\% and 0.2\pm0.03\%$ respectively, the yield of squid pen chitosan was $25\pm3\%$, the degree of deacetylation was $92\%$, and the molecular weight was $1.15\times10^6$, Acetyl contents of N-acetylchitosan powder, acetylchitosan bead, N-ACF-1 (N-acetylchitosan film-1) and N-ACF-2 (N-acetylchitosan film-2) were $55.9\%, 63.2\%, 56\% and 58.7\%$ respectively. Two major peaks, amide I ($1,653 cm^{-1}$) and II ($1,558 cm^{-1}$) bent, on FT-IR spectra of the N-acetylchitosan from squid pen were almost similar to these of $\beta-chitin$, While there was a broad single peak at $1,601 cm^{-1}$assigned to be an amide I bend in squid pen chitosan. The CP/MAS NMR spectra of $\beta-chitin$, squid pen chitosan and N-acetylchitosan from squid pen showed a relative broad and single peak at 74 ppm assigned to fifth carbon (C-5) and third carbon (C-3). In case of $\beta-chitin$ and N-acetylchitosan from squid pen, single peak at 74 ppm was showed as the same of $\beta-chitin$ type.

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Fine Structure of Mature Sperms of Cephalopods (Octopus minor, Octopus ocellatus and Todarodes pacificus) Inhabiting the Korean Waters II (한국 연근해산 두족류 (Octopus minor, Octopus ocellatus and Todarodes pacificus) 성숙정자의 미세구조 II)

  • Kim, Sang-Won;Chang, Nam-Sub
    • Applied Microscopy
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    • v.31 no.4
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    • pp.333-345
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    • 2001
  • The mature sperms of three species of cephalopods (Octopus minar, Octopus ocellatus, Todarodes pacificus) were observed by electron microscopy. The results obtained are as follows: The sperm lengths of Octopus minor and Octopus ocellatus of octopods are long and they are about $390{\mu}m$ and $125\sim130{\mu}m$, respectively, but the sperm length of Todarodes pacificus is short and about $35{\mu}m$. The sperm of Octopus minor has a helical acrosome and a head bent a little like a banana while Octopus ocellatus of octopod has a twisted acrosome and a long rod-shaped head. A number of horizontal stripes are observed as a periodic structure in their subacrosome cavities and dense plugs are formed in the cavities of their heads. On the other hand, the acrosome of Todarodes pacificus is circular cap-shaped, and its head is long and oval. It is notable that two small cavities were observed in its basal acrosome. Juxtanuclear acrosomal materials of high electron density filled the subacrosomal cavity. In the middle piece of mature sperms of Octopus minor and Octopus ocellatus, the mitochondria form the mitochondrial sleeve, but the numbers of mitochondria differ between the species so that they are $11\sim12$ and $8\sim9$, respectively. Meanwhile, in the middle piece of mature sperms of Todarodes pacificus, the mitochondria are separated from the axoneme, forming a mitochondrial spur in which $10\sim13$ mitochondria and some electron dense materials concentrate. The axoneme of Octopus minor, Octopus ocellatus and Todarodes pacificus are of 9+2 type in common, surrounded by 9 coarse fibres. A number of glycogen were observed only in the axoneme of Todarodes pacificus. The coarse fibres were found as far as the main piece of sperm tail in Octopks minor and Todarodes pacificus, while to the end piece of sperm tail in Octopus ocellatus.

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Arthroscopic Transosseous Suture Repair for Bankart Lesion with a Flexible Drill Device - An Experimental and Preliminary Clinical Report - (유연성 천공기를 이용한 Bankart 병변의 골관통식 봉합 - 동물 실험 및 예비 임상 결과 보고 -)

  • Park, Jin-Su;Won, Ye-Yeon;Yoo, Jung-Han;Park, Yong-Wook;Noh, Kyu-Chul;Chung, Kuk-Jin;Kim, Hong-Kyun;Hwang, Ji-Hyo;Lee, Young-Bum;Suh, Il-Woo
    • Clinics in Shoulder and Elbow
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    • v.13 no.1
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    • pp.72-78
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    • 2010
  • Purpose: Too develop a flexible drill device that can be inserted into the shoulder joint so that arthroscopic transosseous suture repair for Bankart lesion is possible. Materials and Methods: We created a device composed of a flexible drill unit and a guide pipe unit. The flexible drill unit was made of flexible multifilament wires (1.2 mm in diameter) that was twisted into one cord so that it can flex in any direction and a drill bit (1.2 mm in diameter) that is attached onto one end of the flexible wire. The guide pipe unit was a 150 mm long metal pipe (2.0 mm in inner diameter and 3.0 mm in outer diameter), with one end bent to 30 degrees. The flexible drill set was inserted into the shoulder joint through the posterior portal of the joint. The guide pipe component was placed onto the medial wall of the glenoid so that the pipe was placed 5 mm posterior to the margin of the anterior glenoid rim. The flexible drill was driven through the glenoid by the power drill so that holes were made in the glenoid. A non- absorbable suture was passed through the hole. Tying of a sliding knot tying was accomplished over the capsule and labrum after making a stitch through the capsule and labrum with a suture hook loaded with suture passer. The same procedures were done at the 2 and 4 O'Clock positions of the glenoid. Results: Five cases with Bankart lesion received arthroscopic transosseous repair with our flexible drill device. There were no intraoperative problems. Neither redislocation nor subluxation was reported at final follow-up. Conclusion: Arthroscopic transosseous suture repair without suture anchors and easy tying of a sliding knot are possible with a flexible drill set.

Effect of Seeding Rates on Turf Vegetation of Creeping Bentgrass (파종량이 Creeping Bentgrass 잔디초지의 식생에 미치는 영향)

  • Cho, Nam-Ki;Kang, Young-Kil;Song, Chang-Khil;Cho, Young-Il;Park, Sung-Jun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.25 no.2
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    • pp.131-136
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    • 2005
  • This study was conducted from March 21 to July 7 in 2004 at JeJu Island to investigate the influences of seed-ing rates (4, 6, 5, 10 and 12 kg/10a) on creeping bentgrass vegetation. The result obtained were summarized as follows; plant height was getting longer as seeding rate increased from 4 to 12 kg/10a, Although it was no significance from 10 to 12 kg/10a. Root length, Minolta SPAD-502 chlorophyll reading value, leave and root weight increased as the plant height increased. The degree of land cover and density of creeping bentgrass also increased as seeding rate increased from 4 to 12 kg/10a, and the degree of land cover and density of weed decreased. The number of weed species on decreased as increasing of seeding rate. Then ranking of the dominant weeds were Digitaria adscendens, Chenopodium album var. centrorubrum md Poa annua (at 4 kg/10a seeding rate), Digitaria adscendens, Chenopodium album var. centrorubrum and Stellaria media (at 6 kg/10a seeding rate). Chenopdium album var. centrorubrum, Poa annua and Digitaria adscendens (at 8 kg/10a seeding rate), Digitaria adscendens, Chenopdium album var. centrorubrum and Steilaria media (at 10 kg/10a seeding rate), Chenopdium album var. centrorubrum, Digitaria adscendens and Stellaria media (at 12 kg/10a seeding rate). These results showed that the optimum seed-ing rate is 10 kg/10a for growth of creeping bentgrass in volcanic ash soils of Juju island.

Exploratory Case Study for Key Successful Factors of Producy Service System (Product-Service System(PSS) 성공과 실패요인에 관한 탐색적 사례 연구)

  • Park, A-Rum;Jin, Dong-Su;Lee, Kyoung-Jun
    • Journal of Intelligence and Information Systems
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    • v.17 no.4
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    • pp.255-277
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    • 2011
  • Product Service System(PSS), which is an integrated combination of product and service, provides new value to customer and makes companies sustainable as well. The objective of this paper draws Critical Successful Factors(CSF) of PSS through multiple case study. First, we review various concepts and types in PSS and Platform business literature currently available on this topic. Second, after investigating various cases with the characteristics of PSS and platform business, we select four cases of 'iPod of Apple', 'Kindle of Amazon', 'Zune of Microsoft', and 'e-book reader of Sony'. Then, the four cases are categorized as successful and failed cases according to criteria of case selection and PSS classification. We consider two methodologies for the case selection, i.e., 'Strategies for the Selection of Samples and Cases' proposed by Bent(2006) and the seven case selection procedures proposed by Jason and John(2008). For case selection, 'Stratified sample and Paradigmatic cases' is adopted as one of several options for sampling. Then, we use the seven case selection procedures such as 'typical', 'diverse', 'extreme', 'deviant', 'influential', 'most-similar', and 'mostdifferent' and among them only three procedures of 'diverse', 'most?similar', and 'most-different' are applied for the case selection. For PSS classification, the eight PSS types, suggested by Tukker(2004), of 'product related', 'advice and consulancy', 'product lease', 'product renting/sharing', 'product pooling', 'activity management', 'pay per service unit', 'functional result' are utilized. We categorize the four selected cases as a product oriented group because the cases not only sell a product, but also offer service needed during the use phase of the product. Then, we analyze the four cases by using cross-case pattern that Eisenhardt(1991) suggested. Eisenhardt(1991) argued that three processes are required for avoiding reaching premature or even false conclusion. The fist step includes selecting categories of dimensions and finding within-group similarities coupled with intergroup difference. In the second process, pairs of cases are selected and listed. The second step forces researchers to find the subtle similarities and differences between cases. The third process is to divide the data by data source. The result of cross-case pattern indicates that the similarities of iPod and Kindle as successful cases are convenient user interface, successful plarform strategy, and rich contents. The differences between the successful cases are that, wheares iPod has been recognized as the culture code, Kindle has implemented a low price as its main strategy. Meanwhile, the similarities of Zune and PRS series as failed cases are lack of sufficient applications and contents. The differences between the failed cases are that, wheares Zune adopted an undifferentiated strategy, PRS series conducted high-price strategy. From the analysis of the cases, we generate three hypotheses. The first hypothesis assumes that a successful PSS system requires convenient user interface. The second hypothesis assumes that a successful PSS system requires a reciprocal(win/win) business model. The third hypothesis assumes that a successful PSS system requires sufficient quantities of applications and contents. To verify the hypotheses, we uses the cross-matching (or pattern matching) methodology. The methodology matches three key words (user interface, reciprocal business model, contents) of the hypotheses to the previous papers related to PSS, digital contents, and Information System (IS). Finally, this paper suggests the three implications from analyzed results. A successful PSS system needs to provide differentiated value for customers such as convenient user interface, e.g., the simple design of iTunes (iPod) and the provision of connection to Kindle Store without any charge. A successful PSS system also requires a mutually benefitable business model as Apple and Amazon implement a policy that provides a reasonable proft sharing for third party. A successful PSS system requires sufficient quantities of applications and contents.

Herbicidal Activities of Dinitroaniline Compounds in Turfgrass (Dinitroaniline계(系) 제초제(除草劑)의 잔디밭에서의 작용특성(作用特性))

  • Lee, Y.D.;Kim, S.J.;Kim, K.W.;Kim, K.U.
    • Korean Journal of Weed Science
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    • v.14 no.4
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    • pp.265-271
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    • 1994
  • This study was conducted to determine the herbicidal activity, persistance, downward movement and effect on bentgrass of 7 dinitroaniline herbicides such as benefin [N-butyl-N-ethyl-2,6-dinitro-4-(trifluoromethyl) benzenamine], beslogine [N,N-dibutyl-2,6-dinitro-4-trifluoromethylaniline], prodiamine [2,4-dinitro-$N^3$,$N^3$-dipropyl-6-(trifluoromethyl)-1,3-benzenediamine], pendimethalin [N-(1-ethylpropyl)-3,4-dimethyl-2,6-dinitrobenzenamine], trifluralin [2,6-dinitro-N,N-dipropyl-4-(trifluoromethyl) benzenamine], ethalfluralin [N-ethyl-N-(2-methyl-2-propenyl)-2,6-dinitro-4-(trifluoromethyl) benzenamine], and oryzalin [4-(diprop-ylamino)-3,5-dinitrobenzene-sulfonamide) together with bensulide [O,O-bis(1-methylethyl) S-[2-[(phenyl-sulfonyl) amino]ethyl]phosphorodithioate] and siduron [N-(2-methylcyclohexyl)-N'-phenylurea) as the control. In addition, distribution of pendimethalin and benefin into bentgrass was also determined. Prodiamine, benefin, and pendimethalin at the 1/16 dose of the recommended rate showed very high herbicidal activity($LD_{90}$) on Digitaria sanguinalis, but ethalfluralin and bensulide showed $LD_{90}$ at the 1/4 dose of the recommended rate, showing difference in herbicidal activities among dinitroaniline herbicides. All the herbicides except for pendimethalin had the lower herbicidal activity in sandy soil than that of clay-loam soil. Benefin, beslogine, prodiamine, oryzalin, bensulide and siduron persisted in the soil for about 50 days, but pendimethalin persisted in the soil for about 35 days, and trifluralin and ethalfluralin for about 25 days. Ethalfluralin, oryzalin and bensulide were the most mobile(downward movement) of the 9 herbicides studied. Less mobility was observed in the turfgrass condition than that of the bare soil. Beslogine bensulide prodiamine and benefin had no injury effect on bentgrass(Agrastis atolonifera L., penncross creeping bent grass). However, herbicides like oryzalin, trifluralin and pendimethalin reduced the dry weight of bentgrass by 12%, 30% and 40%, respectively. No significant difference in distribution of pendimethalin and benefin into inner part of leaves, surface and wax layer of bentgrass was observed, and thus it seems that different phytotoxic effect between pendimethalin and benefin may be attributed to different metabolism and mode of action.

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Occurrence of Culex pipiens (Diptera, Culicidae) and Effect of Vent Net Sets for Mosquito Control at Septic Tanks in South-eastern Area of the Korean Peninsula (한반도 동남부 지역 정화조 내의 빨간집모기군(群)(Culex pipiens complex) 발생과 정화조 환기통의 방충망 설치에 의한 방제효과)

  • Lee Dong-Kyu
    • Korean journal of applied entomology
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    • v.45 no.1 s.142
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    • pp.51-57
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    • 2006
  • Culex pipiens complex larvae were found at the septic tanks in Busan and Ulsan cities located in South-eastern area of Korean peninsula. The larvae were collected in 25, 35 and 75 septic tanks out of 3,527, 622 and 1,111 septic tanks which the breeding rates were averages of 0.7%, 5.6% and 6.8% in Busan from January to May, 2003; Busan from February to March, 2004; and Ulsan from January to April, 2005, respectively. The average numbers of the mosquitoes in the tanks were 38.8, 14.9 and 13.7 larvae/dip with a 355-ml dipper. The larval densities were from 25.4 larvae/dip in January to 43.7 larvae/dip in April. 2003 which were not significantly different between them. It was the first report to find mosquito breeding at septic tink in Republic of Korea. Culex pipiens larvae were found in 6 out of 379 septic tanks at complex buildings (over $2,000m^2$ in size) in Ulsan from June to September, 2005, having an average of 1.6% for mosquito breeding rate. The mosquito larvae were collected in 8 and 530 septic tanks out of 210 and 3,729 septic tanks at large (over 300 homes) and small apartments (below 300 homes) with 3.8% and 14.2% of the average breeding rates, respectively At the septic tanks of private houses for below 50 persons, 708 out of 7,178 septic tan had Cx. pipiens larvae and pupae with 9.9% of the average breeding rate. Total average of the mosquito breeding rate was 10.9% of the investigated septic tanks of residential buildings in Ulsan. The number of mosquito larvae in the septic tanks were 15 to 75 larvae/dip. The bent net sets of septic tanks prevented from mosquito breeding to the rate of 93.1%.

The Effect of Creeping Bentgrass Growth on Greenspeed (그린잔디의 생육이 그린스피드에 미치는 영향)

  • Kwon, Il-Woo;Lee, Dong-Hee;Choi, Byuong-Man;Tae, Hyun-Sook;Shin, Dong-Hyun
    • Asian Journal of Turfgrass Science
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    • v.25 no.2
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    • pp.223-228
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    • 2011
  • This research was performed to investigate change of green speed according to growth of grass, for this, the method of effective green management for improvement of green speed was searched by investigating green density, soil moisture, surface hardness, and mowing height every day for 6 months. As the result of the study, reliability between, green density, soil moisture, surface hardness, mowing height and green speed were measured to be respectively 0.4742, 0.5690, 0.4632, 0.2806, i.e. soil moisture is considered as the factor which affects green speed the most. Therefore, it will be an advantageous environment to maintain soil moisture a little bit low to improve green speed within the range that does not disrupt the growth of green. In case of green density, it is considered to be effective to get a fast green speed when obtaining enough density during May~June, the most vigorous growth period and at the same time green up period. Surface hardness was confirmed that management work as rolling is a considerably effective method to increase hardness. However, rolling gives high stress to the green, combining another management work as regular hilling could be a good alternative. Reliability of green preview and green speed was 0.2806, lower than soil moisture or surface hardness. Through the results, it was confirmed that management of mowing height to be low less than 3.00 mm is helpful to improve green speed, timely, and it is advantageous to manage green speed when adjusting mowing height during the vigorous growth period of bent grass. However, considering the range of mowing height was not various, being 2.9~3.4 mm, henceforth research on investigation of green speed at more various mowing heights would be necessary. Consequently, except mowing height, other three factors, i.e. green density, soil moisture and surface hardness were investigated to have considerable level of reliability on green speed, and it is considered that each factor affects green speed respectively according to green condition and time. Accordingly, in order for the manager to maintain high speed all year round, intensive care for each factor per time unit considering green growth condition is considered to be necessary.

Further Studies on the Specificity of the N- and C-terminal Antigenic Determinant of Hen Egg-white Lysozyme (계난백(鷄卵白) Lysozyme의 N-말단(末端)과 C-말단(末端) 항원결정기(抗原決定基)에 대한 연구(硏究))

  • Ha, Youn-Mun
    • The Journal of the Korean Society for Microbiology
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    • v.12 no.1
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    • pp.19-32
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    • 1977
  • The specificity of the N- and C-terminal antigenic determinant($P_{17}$: sequence $Lys^1-{cys-}^6-Asn^{27},\;{Trp^{12}}_2-Cys^{127}-Leu^{129}$) of hen egg-white lysozyme(HL) was studied in more detail. In a Scatchard plot of the binding of $^{14}C$-acetyl HL with guinea pig purified anti-$P_{17}$ antibody experimental values bent sharply aear r=1. This suggests of two antibody populations with different affinities for HL or possible steric hindrance in the binding of a second HL molecule to the second binding site of the antibody molecule. The antigenic activities of various peptides were tested by measuring their inhibition of the binding of $^{14}C-acetyl-P_{17}$ with the antibody, Only $P_{17}$ and $P_{17}t$(sequence $Lys^1-cys^6-Homoser^{12},\;Trp^{123}-Cys^{127}-Leu^{128})$) were inhibitory, with $K_1$ values of $2.0{\times}10^4$ and $8.1{\times}10^3$, respectively. These results indicate that the direct binding site of $P_{17}$ to anti-$P_{17}$ antibody may be located in the terminal portion of $P_{17}$ (sequence $Lys^1-Cys^6-Homoser^{12},\;Trp^{123}-Cys^{127}-Leu^{129})$) while the rest of $P_{17}$ may be important in maintaining the conformation of this determinant. The single disulphide bond involved in this determinant is essential for manifestation of immunological activity.

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Bond strength of fiber reinforced composite after repair (섬유 강화 컴포지트의 수리 후 접합 강도)

  • Kim, Min-Jung;Kim, Kyung-Ho;Choy, Kwang-Chul
    • The korean journal of orthodontics
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    • v.36 no.3 s.116
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    • pp.188-197
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    • 2006
  • Fiber reinforced composite (FRC) is usually used as a connector joining a few teeth into one unit in orthodontics. However, fracture often occurs during the two to three years of the orthodontic treatment period due to repeated occlusal loading or water sorption in the oral environment. We simulated the repair by overlapping and attaching portions of two FRC strips in the middle and performed a three-point bending test to investigate the changes of the repair strength among the different FRC groups. The specimens were grouped according to the overlapping lengths of the two FRC strips, which were 1, 2, 3 and 4 mm (group E1, E2, E3 and E4, respectively) and the control group consisted of unrepaired, intact FRC strips. Each group consisted of 6 specimens and were cured with a light emitting diode curing unit. Group E4 showed the highest maximum loads of 2.67 N, then the control group (2.39 N), group E3 (2.35 N), E2 (2.10 N), and E1 (1.75 N) in decreasing order. Group E4 also showed the highest stiffness, which was 2.32 N/mm, however, the stiffness of group E3 (2.06N/mm) was higher than that of the control group (1.88 N/mm). According to the visual examination, the specimens tended to be bent rather than being fractured into two pieces with an increased length of overlapping portions. The above results suggest that a minimum overlapping length of 3 mm was necessary to obtain an adequate repair of a 10 mm length of FRC connector. In addition, the critical section adjacent to the joint area, where the thickness decreased abruptly, should be reinforced with flowable resin to minimize the bending tendency.