• Mycobiology
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• v.35 no.4
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• pp.219-225
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• 2007

A keratinolytic enzyme secreted by Aspergillus flavus K-03 cultured in feather meal basal medium (FMBM) containing 2% (w/v) chicken feather was purified and characterized. Keratinolytic enzyme secretion was the maximal at day 16 of the incubation period at pH 8 and $28^{\circ}C$. No relationship was detected between enzyme yield and increase of fungal biomass. The fraction obtained at 80% ammonium sulfate saturation showed 2.39-fold purification and was further purified by gel filtration in Sephadex G-100 followed by ion exchange chromatography on DEAE-Sephadex A-50, yielding an active protein peak showing 11.53-fold purification. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and zymograms indicated that the purified keratinase is a monomeric enzyme with 31 kDa molecular weight. The extracellular keratinase of A. flavus was active in a board range of pH ($7{\sim}10$) and temperature ($30^{\circ}C{\sim}70^{\circ}C$) profiles with the optimal for keratinase activity at pH 8 and $45^{\circ}C$. The keratinase activity was totally inhibited by protease inhibitors such as phenylmethylsulfonyl fluoride (PMSF), iodoacetic acid, and ethylenediaminetetraacetate (EDTA) while no reduction of activity by the addition of dithiothreitol (DTT) was observed. N-terminal amino acid sequences were up to 80% homologous with the fungal subtilisins produced by Fusarium culmorum. Therefore, on the basis of these characteristics, the keratinase of A. flavus K-03 is determined to be subtilisins-like.

• Tribology and Lubricants
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• v.23 no.5
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• pp.234-239
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• 2007

Recently one of the automobile customer's greatest concerns is quietness in a vehicle along with various functional requirements such as comfort, stability, mobility etc. Therefore car makers place more weight on vehicle noise. Especially, in the case of diesel engine, as the noise level of engine becomes more silent by the development of engineering technology. The noise of alternator with vacuum pump has been able to be noticed enough becoming an issue on vehicles. In this study vacuum pump noise on alternator was identified andclassified into five types. DOE was applied to induce the statistical analysis result to reduce the vacuum pump noise. It was done by subjective listening. Design and statistical analysis are done with Minitab software. This work provides dominant elements of vacuum pump noise on alternator and can be the basis of furture studies.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.4
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• pp.760-765
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• 1999

Changes in physiochemical properties of Sunchang sikhe kochujang, the most famous traditional kochujang, by red ginseng addition(1, 2 and 5% red ginseng on the total weight basis) were investigated. Measurements of physiochemical parameters such as reducing sugar content, alcohol content, amino type nitrogen content and color difference value were conducted during fermentation at 25oC for 120 days. Alcohol content was increased from the begining of fermentation, reaching at the highest level after 90 days of fermentation and then slowly reduced. Alcohol content of red ginseng kochujang was generally higher than that of control kochujang after 90 days of fermentation. Crude protein content of control kochujang and red ginseng kochujang were reduced during fermentation whereas amino type nitrogen content were gradually increased during fermentation. Amino type nitrogen content of red ginseng kochujang appeared to be slightly lower than that of the control kochujang. The reduction in amino type nitrogen content of red ginseng kochujang was negatively related to the level of red ginseng addition. Among color difference values, L and b value of both control kochujang and red ginseng kochujang were reduced by 30 days from the begining of fermentation and then started to be increased.

• Child Health Nursing Research
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• v.9 no.1
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• pp.96-106
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• 2003

Advances in neonatal care system and research have resulted in an increased survival rate among low birth weight infants in the industrialized countries. Recent Korean neonatal mortality and morbidity statistics, and current status of Korean neonatal intensive care facilities were reviewed here for the sake of future improvement and research. Morbidity statistics revealed that perinatal diseases accounted for 80％ of the death of premature infants implying the possibility of its reduction by the vigorous prenatal care service in future.On the basis of extensive studies of nutritional support and growth rate of premature infants, commercial formulas for premature infants have been developed and various aspects of feeding techniques are standardized. However, problem of growth deficit of premature infants remains unsolved as medical problem. NICU specialists are challenged with the tasks of prenatal education or provision of care that minimizes the neurodevelopmental problems seen in preterm infants, various short-term outcome researches have been reported and those are reviewed here to promote research interest in the field of neonatal nursing. Systemic long-term outcome studies are also awaited in Korea for the formulation of welfare policy in future. Nursing science has to embrace all these interdisciplinary studies as their own research field in collaboration with neonatologist, nutritionist, rehabilitation therapist, social workers and teachers.

• Journal of the Society of Naval Architects of Korea
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• v.46 no.2
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• pp.179-188
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• 2009

WIG crafts are a high speed vessel with features of dynamic supported craft. These crafts, which are predominantly of light weight and operate any substantially greater speeds than conventional craft such as bulk carrier, tanker, container ship, etc., could not be accommodated under traditional maritime safety instruments. It means that there is the need for risk and safety levels to be assessed on a holistic basis, recognizing that high levels of operator training, comprehensive and thoroughly implemented procedures, high levels of automation and sophisticated software can all make significant contributions to risk reduction. To response this requirement, the Interim Guideline for WIG craft(MSC/Circ.1054) were developed in the view of the configuration of WIG craft, which fall between the maritime and aviation regulatory regimes. This paper reviews a safety assessment process and methodology to be used in the design phase of a new ship. The process and methodology is based on the risk-based approach and is applied to safety assessment in concept development phase of small WIG craft in the 20-person class.

• Journal of Technologic Dentistry
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• v.11 no.1
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• pp.95-102
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• 1989

This study was performed to study the efficiency of a local ventilation system, installed in dental laboratories, and of two types of protecting face masks. The dust originating from the workpiece as well as from the wheels and stones was collected on air filters in the cutting cycle during coarse and fine grinding, and in the subsequent polishing procedures of each specific dental material. The efficiency of the ventilation system was measured on the basis of weight reduction of dust in the breathing air at a distance about 20-40 cm from workpiece. The results were as follows: 1. Use of the local ventilation system reduced the amounts of respirable dust to an average level of 21.4%, although the efficiency of the local ventilation system varied depending upon materials used. 2. Mounting a nozzle on the tube improved the efficiency of the ventilation system considerably. The efficiency of the local ventilation system also increased as the workpiece was closing to the tube inlet. 3. With or without the local ventilation system, the distance between the position of the workpiece and sampler greatly affected the dust level. 4. The face masks covering the sampler improved the efficiency of the ventilation system considerably.

• Textile Coloration and Finishing
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• v.15 no.3
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• pp.132-139
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• 2003

PTT[Poly(trimethylene terephthalate)] fibers was annealed by passing on the plate heater to illuminate the effects of annealing on the alkaline hydrolysis behavior properties with varying the treatment temperature for 0.5 second. The L010 and crystallinity were increased with increases in temperature. With the increases of the temperature, the dynamic viscoelastic behaviors were analyzed to be reduction in $T_{max}(tan\;\delta)$. The weight loss in alkaline solution was two times more rapid for the PTT annealed at $200^\circ{C}$ than the control samples. The kinetics of hydrolysis was confirmed that the hydrolysis of the PTT fibers in the alkaline solution was started from the surface of the fibers and selective to the amorphous region in continuation, on the basis of the results of the increase in crystallinity and the decrease in dye uptake at the initial stage of the hydrolysis.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.11 no.1
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• pp.33-40
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• 2005

This study was carried out for effective utilization of recycling resources to investigate the repulping conditions of car air filter waste paper and to evaluate the application into corrugating medium papermaking by blending these repulped pulps. Car air filter waste paper was made of virgin BKP and it was dipped into phenol resin solution. It was well disintegrated by laboratory Valley beater with 10%(basis on oven-dried pulp weight) NaOH addition and defoamer usage. The optimal temperature, beating consistency and treatment time were mainly $40^{\circ}C$, 1% and $30{\sim}40$ minutes, respectively. Handsheets were prepared with various blending ratios between air filter recycled pulp and KOCC. In the case of $10{\sim}20%$ substitution with air filter recycled pulp, physical properties reductions as compressive strength and burst strength of sheets were lower than others. These results showed more favour than the partial substitution of KOCC for corrugating medium even though some strength reduction of paper. It was also observed that the waste water of air filter recycling was not affective to environmental problems.

• Food Science and Biotechnology
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• v.15 no.4
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• pp.606-611
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• 2006

The chemical composition of Smilax china leaves and the quality characteristics of seolgitteok (rice cake) prepared with a water extract of these leaves were evaluated. Sucrose, glucose, maltose, and fructose were found as free sugars in the leaves, while the main fatty acids were stearic and palmitic acids. Glutamic acid and potassium were found at the highest levels among the analyzed amino acids and minerals in the leaves, respectively. On a fresh weight basis, the content of total phenolics and condensed tannin was 1.26 and 0.74%, respectively. As the amount of S. china leaf extract increased, the lightness of seolgitteok significantly decreased (p<0.001) in the rice cake, while redness and yellowness of the cake increased. Texture evaluation showed that springiness, strength, and cohesiveness were higher in rice cakes prepared with 2% S. china leaf extract compared to rice cakes made with 1% extract. At the beginning of the storage period ($20^{\circ}C$), there were no significant differences in viable aerobic cell and mold counts among rice cakes, but after four days in storage, a significant reduction in microorganisms was observed in rice cakes prepared with increasing amounts of leaf extract. On sensory evaluation, rice cakes made with 1% water extract from S. china leaves scored the highest on flavor, taste, texture, and overall acceptability.

• Journal of Korean Physical Therapy Science
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• v.28 no.3
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• pp.76-87
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• 2021

Background: The purpose of this study was to evaluate the effects of power walking and square dancing on middle-aged women with hypertension. Design: Randomized controlled Trial. Methods: 30 middle-aged women with hypertension were selected and divided into two groups. 15 cases in the control group received routine treatment, and 15 cases in the intervention group received community vigorous walking and square dancing intervention on the basis of routine treatment. The intervention time was 40-60minutes/day, 5days/week, total 16 weeks. total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL), low density lipoprotein cholesterol (LDL), angiotensin II (Ang II), Leptin, blood pressure, and heart rate were measured. Results: Body weight, body mass index (BMI), TC, TG, LOW-density lipoprotein, angiotensin II, leptin, systolic blood pressure and heart rate were significantly reduced after power walking and square dancing (p<0.05). After the experiment, TC and TG in the experimental group were lower than those in the control group (p<0.05), while HDL was higher (p<0.05). Conclusion: The results of this study suggest that power walking exercises and square dances are significant effects on lipid mechanism and heart rate.