• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 한국소음진동공학회 2004년도 추계학술대회논문집
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• pp.375-380
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• 2004

Seismic Isolation and Shock/vibration Control Laboratory has performed the National Research Laboratory(NRL) project, 'Design and Application of Control Devices against Earthquake/Shock/Vibration'. In this project, the prototypes of the vibration control devices for structural control against earthquake and wind were developed and verified their performances. And also, the computer programs were developed for the seismic response analysis and the optimum design of the base-isolated structures with vibration control devices. This paper introduces the developed vibration control devices and computer programs.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 한국지진공학회 2006년도 학술발표회 논문집
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• pp.463-470
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• 2006

이 논문에서는 신경망 제어기와 MR 댐퍼를 이용하여 지진하중을 받는 지진격리 벤치마크 구조물의 응답 감소를 위한 반능동 제어방법이 제안되었다. 제안방법 중 신경망 제어기에는 적절한 제어력을 산출하기 위해 가격함수를 기반으로한 학습 알고리즘과 간편한 민감도 계산기법이 도입되었다. MR 댐퍼가 계산되어진 제어력과 비슷한 제어력을 낼 수 있도록 clipped 알고리즘을 이용하였고, 제안된 반능동 신경망 제어방법이 지진격리 장치가 설치된 벤치마크 구조물에 적용되었다. 수치해석에서는 벤치마크 문제를 정의한 논문에서 제공된 수동제어방법이나 예시제어방법과 제안 방법의 제어성능을 비교하였다. 수치해석 결과 제안방법은 지하 변위를 약간 증가시키지만, 각층의 가속도, base shear, building corner drift 등을 매우 효과적으로 줄이는 것으로 판명되었다.

• BMB Reports
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• 제32권5호
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• pp.436-439
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• 1999

Shigella sonnei is an important cause of human enteric infections. S. sonnei KNIH104S was previously reported to be isolated from Korean shigellosis patients. We cloned a 2.8-kb KpnI fragment containing the recA gene encoding a recombinase from the chromosomal DNA of S. sonnei KNIH104S. This recombinant plasmid was named pRAK28. E. coli HB101, a recA mutant, cannot grow on Luria-Bertani medium in the presence of the alkylating agent methylmethane sulfonate, however, E. coli HB101 harboring pRAK28 was found to grow on this medium. As far as we know, we are the first to sequence the recA gene from S. sonnei. This gene is composed of 1062 base pairs with an ATG initiation codon and a TAA termination codon. Nucleotide sequence comparison of the S. sonnei recA gene exhibited 99.7% and 99.5% identity with those of S. flexneri and E. coli, respectively.

• Proceedings of the KIEE Conference
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• 대한전기학회 1989년도 하계종합학술대회 논문집
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• pp.237-240
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• 1989

This paper deals with the expert system for power system recognition of energized and deenergized system using circuit breaker information. The basic idea is isolating the system with the system matrix representing the system configuration and the states of the circuit breakers. The knowledge base is composed of these isolated systems and decision rules. The isolated system with the sources is recognized as the energized system and the system without the source as the deenergized system. The rules use the system matrix and the the inference scheme is simplified in a great deal. Above all, the overall searching labor of the rules is independent on the system size and it is possible to expand into the real system and the real time restoration can be carried out easily. The expert system is written in PROLOG.

• Journal of Korean Association for Spatial Structures
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• 제10권2호
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• pp.69-76
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• 2010

After obtaining tensile and compressive stiffness as well as shear stiffness of elastomeric seismic isolator experimentally, those stiffness were modeled analytically using nonlinear computer program. To induce tensile stress due to overturning in the seismic isolators of an isolated frame for horizontal force, free vibration simulations generated by large initial displacement were conducted. Since elastomeric seismic isolator is weak for tensile stress, the axial stiffness of isolators shall be included properly in the analytical model to evaluate the uplift phenomenon of elastomeric seismic isolator.

• Structural Engineering and Mechanics
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• 제67권1호
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• pp.69-78
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• 2018

The study presented herein focused on the change in hysteretic force-deformation behavior of lead rubber bearings (LRBs). The material model used to idealize response of LRBs under cyclic motion is capable of representing the gradual attrition in strength of isolator unit on account of lead core heating. To identify the effect of loading history on the hysteretic response of LRBs, a typical isolator unit is subjected to cyclic motions with different velocity, amplitude and number of cycles. Furthermore, performance of an LRB isolated single degree of freedom system is studied under different seismic input levels. Finally, the significance of lead core heating effect on LRBs is discussed by considering the current design approach for base isolated structures. Results of this study show that the response of an LRB is governed strongly by the amplitude and number of cycles of the motion and the considered seismicity level.

• Wind and Structures
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• 제9권5호
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• pp.357-367
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• 2006

Unsteady 3D Reynolds Averaged Navier-Stokes (URANS) solver is used to simulate the turbulent flow past an isolated prismatic cylinder at Re=37,400. The aspect ratio of height to base width of the body is 5. The turbulence closure is achieved through a non-linear $k-{\varepsilon}$ model. The applicability of this model to predict unsteady forces associated with this flow is examined. The study shows that the present URANS solver with standard wall functions predicts all the major unsteady phenomena showing closer agreement with experiment. This investigation concludes that URANS simulations with the non-linear $k-{\varepsilon}$ model as a turbulence closure provides a promising alternative to LES with view to study flows having complex features.

• Microbiology and Biotechnology Letters
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• 제28권4호
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• pp.219-222
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• 2000

To produce and utilize microbial cyclomaltodextrinase being industrially useful, we isolated an alkalophilic Bacillus strain from soil which was capable of degrading cyclodextrins. The newly isolated strain was aerobic, gram-positive, spore-forming, motile, rod shape(0.2~0.4$\times$1.4~4.4 $\mu\textrm{m}$), and 35.8 mol% of DNA base composition. Based on its morphological, phisiological, and biochemical properties, it was identified as alkalophilic Bacillus sp. KJ-133 and cultivated well in the ranges of $30~40^{\circ}C$ and pH 8.0~9.0 . The cyclomaltodextrinase of the strain showed maximal production after 48h of cultivation at $37^{\circ}C$, and the activity was inhibited by Ag2+, Hg2+, Cu2+, and p-chloromercuribenzoate.

• Microbiology and Biotechnology Letters
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• 제25권3호
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• pp.277-285
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• 1997

Kluyveromyces marxianus var. marxianus isolated as an inulin-assimilating microorganism produces inulin fructotransferase (inulaseII) which catalyses the conversion of inulin into di-D-fructofuranose 1, 2' : 2, 3' dianhydrde (DFAIII). The DFA produced by the organism was isolated by using active carbon column, and identified as DFAIII by high performance liguid chromatography. The culture medium giving maximum inulaseII production was found to consist of 1% sucrose and 0.75% yeast nitrogen base (YNB). The inulasell production was induced by inulin or sucrose as a carbon source and increased by addition of YNB as a nitrogen source. Optimal initial pH of the culture medium, culture temperature and medium volume for the enzyme production were pH 4.7, 30$\circ$C and 140 ml, respectively. Under the optimal conditions described above, the enzyme activity in the culture supematant reached 4.2 units/ml after cultivation for 36 h. The DFAIII was accumulated at 13.25 mg/ml after 48 h of culture in the Jerusalem artichoke tuber medium.

• Environmental Engineering Research
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• 제23권1호
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• pp.62-69
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• 2018

In the present study, long-term heavy metals (HMs) contaminated soil samples from a well-known Pb/Zn smelting area in the southwest of China were collected, and physicochemical and biological characteristics of these samples were evaluated. Soil samples contained different concentrations of HMs, namely Pb, Zn, Cu, and Cd. Enzyme activity analyses combined with microcalorimetric analysis were used for soil microbial activity evaluation. Results showed that two soil samples, containing almost the highest concentrations of HMs, also shared the greatest microbial activities. Based on correlation coefficient analysis, high microbial activity in heavily HMs contaminated soil might be due to the high contents of soil organic matter and available phosphorus in these samples. High-throughput sequencing technique was used for microbial community structure analysis. High abundance of genera Sphingomonas and Thiobacillus were also observed in these two heavily contaminated soils, suggesting that bacteria belonging to these two genera might be further isolated from these contaminated soils and applied for future studies of HMs remediation. Results of present study would contribute to the evaluation of microbial communities and isolation of microbial resources to remediate HMs pollution.