• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.161-170
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• 2017

Meningitis caused by Streptococcus suis serotype 2 (S. suis 2) is a great threat to the pig industry and human health. Virulence factors associated with the pathogenesis of meningitis have yet to be clearly defined, even though many potential S. suis 2 virulence factors have been identified. This greatly hinders the progress of S. suis 2 meningitis pathogenesis research. In this study, a co-culture blood-brain barrier (BBB) model was established using primary porcine brain microvascular endothelial cells and astrocytes, and the whole genome library of S. suis 2 was constructed using phage display technology. Finally, a total of 14 potential virulence factors contributing to S. suis 2 adherence to and invasion of the BBB were selected by analyzing the interactions between the phage library and the co-culture model. Twelve of these factors have not been previously reported in meningitis-related research. The data provide valuable insight into the pathogenesis of S. suis 2 meningitis and potential targets for the development of drug therapies.

• 한국토양비료학회지
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• 제47권6호
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• pp.398-405
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• 2014

Salt accumulation at soil surface is one of the most detrimental factors for crop production in reclaimed tidal land. This study was conducted to investigate the effect of capillary barriers beneath the soil surface on dynamics of soil salts at coarse-textured reclaimed tidal land. A field experiment was conducted at Saemangeum reclaimed tidal land for two years (2012-2013). Capillary barriers ($3.5{\times}12m$) were treated with crushed-stone, oyster shell waste, coal briquette ash, coal bottom ash, rice hull and woodchip at 40-60 cm depth from soil surface. Silage corn (Zea mays) was cultivated during the experimental period and soil salinity was monitored periodically. Soil salinity was significantly reduced with capillary barrier compared to that of control. Oyster shell waste was one of the most effective capillary barrier materials to control soil salinity at Saemangeum reclaimed tidal land. At the first growing season capillary barrier did not influence on corn growth regardless of types of the material, but plant biomass and withering rate of corn were significantly improved with capillary barrier at the second growing season. The results of this study showed that capillary barrier was effective on the control of soil salinity and improvement of corn growth, which indicated that capillary barrier treatment can be considered one of the best management practices for stable crop production at Saemangeum reclaimed tidal land.

• 한국의류학회지
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• 제32권9호
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• pp.1450-1460
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• 2008

The goal of this study was to investigate the impacts of service quality, customer satisfaction, and switching burier on store loyalty of the clothes shops at large-scale discount stores. The subjects were 357 female adults living in Suncheon City, Jeollanam Province. The questionnaires were conveniently sampled from June 1 to 30, 2006. The collected data were factor and reliability analyzed using the SPSS program. And Regression was used to verify the relationships between the variables. Among the six hypotheses set in the research model, total three were accepted through empirical analysis and the rest three were accepted partially. The empirical results showed the following managerial implications. First, consumer' perceived service quality has relationship with customer satisfaction and store loyalty. Second, service quality has a positive relationship with switch barrier. Third, customer satisfaction has significantly related with switch barrier. Fourth, switch barrier has positively related with store loyalty. Finally, 'sales people', 'VMD/atmosphere', and 'assortment' of the service quality factors have positive relationships with store loyalty, but 'policy' has a negative relationship with store loyalty.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제2권4호
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• pp.274-278
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• 2021

Road structures play an important role in collisions involving vehicles and wildlife. Our study aimed to determine the effect of various types of road structures on the risk associated with roadkill. We surveyed 50 previously identified roadkill hotspots, ranked from one to five according to roadkill density. We collected nine types of road structure data on each hotspot road section. Structures with similar characteristics were grouped together, resulting in five categories, namely, median barrier, high edge barrier, low edge barrier, speed, and visibility. We examined the existence of each road structure category at each hotspot rank. The cumulative link model showed that the absence of bottom blocked median barrier increased the roadkill hotspot rank. Our study concluded that a visual obstacle in the middle of roads by the median barrier decreases wildlife road crossing attempts and roadkill risk. We suggest that future roadkill mitigation plans should be established considering these characteristics.

• Journal of Microbiology and Biotechnology
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• 제34권4호
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• pp.940-948
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• 2024

Codium fragile has been traditionally used in oriental medicine to treat enterobiasis, dropsy, and dysuria, and it has been shown to possess many biological properties. Atopic dermatitis (AD) is one of the types of skin inflammation and barrier disruption, which leads to chronic inflammatory skin diseases. In the current investigation, the protective effects of C. fragile extract (CFE) on anti-inflammation and skin barrier improvement were investigated. In LPS-stimulated RAW 264.7 cells, nitric oxide generation and the expression levels of interleukin (IL)-1β, IL-4, IL-6, iNOS, COX-2, and tumor necrosis factor-alpha (TNF)-α were reduced by CFE. CFE also inhibited the phosphorylation of NF-κB-p65, ERK, p-38, and JNK. Additionally, CFE showed inhibitory activity on TSLP and IL-4 expression in HaCaT cells stimulated with TNF-α/interferon- gamma (IFN-γ). Enhanced expression of factors related to skin barrier function, FLG, IVL, and LOR, was confirmed. These findings implied that CFE may be used as a therapeutic agent against AD due to its skin barrier-strengthening and anti-inflammatory activities, which are derived from natural marine products.

• 한국전산구조공학회논문집
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• 제21권1호
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• pp.91-101
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• 2008

I형 프리스트레스트 콘크리트 거더 교량의 활하중 분배계수식을 개발하기 위한 구조해석 모델은 해석결과의 적정성과 함께 모델링의 용이성도 동시에 가지고 있어야 한다. 그 이유는 활하중 분배계수식의 개발 과정에서 무수히 많은 횟수의 구조해석이 필요하기 때문이다. 본 연구에서는 기존 연구와 설계실무에서 사용하고 있는 모델들을 비교하여 적정한 구조해석모델을 선정하였다. 또한 수치해석과 재하시험 결과와의 비교를 통하여 방호벽과 가로보의 휨 강성이 활하중분배에 미치는 영향을 분석하였다. 연구결과로서 I형 프리스트레스트 콘크리트 거더 교량의 구조해석에는 편심을 반영한 거더, 방호벽 및 가로보를 바닥판에 연결시킨 모델이 해석결과의 정확성과 모델링의 편이성을 동시에 만족시키는 측면에서 적합하였다. 그러나 방호벽은 강성변화에도 불구하고 활하중분배에 미치는 영향이 미소한 것으로 분석되었다. 편심을 고려한 가로보는 휨 강성 25% 이상에서는 강성변화에 따른 영향이 적었다. 따라서 거더는 바닥판과의 편심을 고려하여 강체요소로 연결하고, 방호벽은 무시하고, 가로보는 전 단면이 유효한 것으로 가정한 상태에서 편심을 주지 않는 모델을 I형 프리스트레스트 콘크리트 거더 교량의 활하중 분배계수식의 개발을 위한 최종 구조해석 모델로서 선정하였다.

• 무역학회지
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• 제43권1호
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• pp.199-221
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• 2018

본 연구에서는 국제벤처기업의 국제화에 영향을 미치는 요인들을 체계화하고, 각 요인의 상대적 중요성을 분석하고자 하였다. 또한, 점진적으로 국제화 과정을 거치는 전통적 기업들과 태생적 국제 벤처기업의 장애요인에 대한 우선순위 차이를 파악하고자 하였으며, 이를 통해 태생적 국제 벤처기업에게 경쟁력을 확보할 수 있는 기초정보를 제공하고자 하였다. 연구방법은 우선순위를 분석하기 위하여 AHP(의사결정 계층 분석 방법)으로 실시하였다. 분석 대상은 각 기업의 국제진출 관련 의사결정권자인 임원들을 대상으로 실시하였으며, 태생적 국제벤처기업 15부, 점진적 수출기업 15부로 각각 조사 하였다. 연구 결과, 1차 장애요인으로 환경 관련 장애요인이 국제화의 가장 큰 장애요소로 인식하고 있었으며, 그 다음으로 기업 관련 장애요인, 경영자 관련 장애요인 순으로 중요성을 인식하고 있다는 점을 확인하였다. 2차 장애요인을 살펴본 결과, 기업관련 장애요인 중 전반적으로 마케팅 역량부족이 가장 큰 장애요인으로 인식하였으며, 이는 점진적 국제 벤처기업 및 태생적 국제 벤처기업 모두 동일하게 나타나고 있다. 그러나 점진적 국제 벤처기업의 경우, 사업실행의 지나친 위험성을 장애요인으로 인식하고 있지만, 태생적 벤처기업의 경우 기술정보 및 R&D 역량의 부족을 장애요인으로 인식하고 있다. 본 연구의 결과는 장애요인을 극복함에 있어서 환경적요인과 기업요인의 결합성이 매우 중요하다는 시사점을 제공하고 있으며, 단순히 내부의 마케팅역량을 강화하는 것만이 국제 벤처기업의 장애요소를 해결하는 것은 아니라는 점이다.

• Journal of Information Technology Applications and Management
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• 제17권3호
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• pp.121-134
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• 2010

Past studies in e-commerce loyalty were mostly focused on the effects of customer satisfaction and trust on loyalty toward online vendors. Few studies investigated the impacts of switching barriers, whilst they are widely proven to affect customer loyalty in offline commerce. Even in a handful of studies that did deal with switching barriers, their treatment of the subject remained at best superficial. This may have to do with the fact that switching costs in e-commerce could be comparatively negligible, as switching to another online vendor often involves one simple mouse click. In this study, we investigated the impact of switching barriers on loyalty under the e-commerce context. Furthermore, the extent of switching barriers which could be affected by those positive factors (most constructs were adopted from IDT) was also examined. The statistical testing results revealed that combined model which includes both the positive factors and the switching barriers explains the loyalty formation process more strongly ($R^2$ = 0.543) than each separated models ($R^2$ = 0.468 for positive factor only model, and $R^2$ = 0.365 for switching barrier only model). While only the two switching barriers such as convenience and emotional were shown to be statistically significant, we found that trust strongly influences customer's emotional barrier, let alone direct impact on loyalty, which thereby influences loyalty. The results offer insights for better understanding switching barriers in e-commerce related applications.

• 농촌지도와개발
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• 제23권1호
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• pp.39-51
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• 2016

The purpose of this study is to examine the female marriage immigrants' social unification through their employment. The subjects of this study were 116 female marriage immigrants who lived in Kyungi-do. This study analyzed employment desire, barrier and volition differences of female marriage immigrants between their socio-demographic factors and effects of their employment desire and barrier to employment volition. Results of this study; First, desire of maintenance of livehood and self esteem to employment incresed with age. Second, there were differences in desire of maintenance of livehood and self esteem to employment between their countries of origins. Third, employment volition and self esteem to employment increased with level of education. Fourth, there were differences in employment volition and self esteem to employment between the length of residence in Korea. Fifth, employment desire and self esteem to employment increased with ability to Korean, but internal barrier of employment decreased by ability to Korean. Sixth, desire of identity from employment and self esteem to employment increased with the number of children. Seventh, employment desire influenced employment volition, self efficacy and self esteem to employment.

• 한국학교보건학회지
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• 제36권1호
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• pp.9-19
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• 2023

Purpose: The purpose of the study was to identify the factors affecting the practice of COVID-19 infection preventive behaviors in university students focusing on the extended health beliefs model (HBM) and the theory of planned behavior (TPB). Methods: Data was collected from November 1, 2021 to November 30, 2021. The participants were 180 university students at 3 universities. The data was analyzed through the independent t-test, one-way ANOVA, Scheffé test, Pearson's correlation coefficient, and multiple regression by using SPSS/WIN 24.0. Results: In the first stage of hierarchical regression analysis, it was shown that perceived barrier and self-efficacy, among the HBM variables, were significant factors, explaining 18.4% of the infection preventive behaviors (F=7.73, p<.001). In the 2nd stage, it was shown that self-efficacy, among the TPB variables, was a significant factor, explaining 15.3% of the infection preventive behaviors (F=11.80, p<.001). In the 3rd stage, it was shown that perceived barrier and self-efficacy, among the HBM and TPB variables, were significant factors, explaining 18.5% of the infection preventive behaviors (F=6.08, p<.001). Conclusion: When an effective infection preventive behavior program is developed and provided for students to improve their self-efficacy and reduce perceived barrier based on the results, it will contribute to the improvement of infection prevention behaviors among university students.