Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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In Vitro Anti-Inflammatory and Skin Protective Effects of Codium fragile Extract on Macrophages and Human Keratinocytes in Atopic Dermatitis

  • A-yeong Jang (Department of Marine Bio Food Science, Gangneung-Wonju National University) ;
  • JeongUn Choi (Department of Marine Bio Food Science, Gangneung-Wonju National University) ;
  • Weerawan Rod-in (Department of Marine Bio Food Science, Gangneung-Wonju National University) ;
  • Ki Young Choi (Department of Marine Bio Food Science, Gangneung-Wonju National University) ;
  • Dae-Hee Lee (Department of Marine Bio Food Science, Gangneung-Wonju National University) ;
  • Woo Jung Park (Department of Marine Bio Food Science, Gangneung-Wonju National University)
  • Received : 2023.12.05
  • Accepted : 2024.01.05
  • Published : 2024.04.28
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Abstract

Codium fragile has been traditionally used in oriental medicine to treat enterobiasis, dropsy, and dysuria, and it has been shown to possess many biological properties. Atopic dermatitis (AD) is one of the types of skin inflammation and barrier disruption, which leads to chronic inflammatory skin diseases. In the current investigation, the protective effects of C. fragile extract (CFE) on anti-inflammation and skin barrier improvement were investigated. In LPS-stimulated RAW 264.7 cells, nitric oxide generation and the expression levels of interleukin (IL)-1β, IL-4, IL-6, iNOS, COX-2, and tumor necrosis factor-alpha (TNF)-α were reduced by CFE. CFE also inhibited the phosphorylation of NF-κB-p65, ERK, p-38, and JNK. Additionally, CFE showed inhibitory activity on TSLP and IL-4 expression in HaCaT cells stimulated with TNF-α/interferon- gamma (IFN-γ). Enhanced expression of factors related to skin barrier function, FLG, IVL, and LOR, was confirmed. These findings implied that CFE may be used as a therapeutic agent against AD due to its skin barrier-strengthening and anti-inflammatory activities, which are derived from natural marine products.

Keywords

Acknowledgement

This research was financially supported by the Ministry of Small and Medium-sized Enterprises (SMEs) and Startups (MSS), Korea, under the "Regional Specialized Industry Development Pius Program (R&D, S3258709)" supervised by the Korea Technology and Information Promotion Agency for SMEs (TIPA). In addition, this research was supported by the University Emphasis Research Institute Support Program (No. 2018R1A61A03023584) funded by the National Research Foundation of Korea.

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