• 환경생물
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• 제28권2호
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• pp.79-85
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• 2010

To examine the effect of Codium fragile on blood cholesterol and lipid metabolism, hyperlipidemia was induced in experimental animal rats through the administration of a hypercholesterolemic diet. Codium fragile powder was then administered to the rats for 5 weeks, after which, blood biochemical changes such as blood cholesterol, Aspartate Aminotransferase (AST: serum SGOT) and Alanine Aminotransferase (ALT: serum SGPT) enzyme activity, etc. were determined. And histological changes in liver cells were examined using an electron microscope. Codium fragile treatment resulted in a significant reduction of the levels of total cholesterol, blood triglyceride and low-density cholesterol (LDL. Chol) compared to the control rats. In contrast the expression levels of high-density cholesterol (HDL. chol.) were increased. The AST value of the Codium fragile administration group was significantly reduced and the blood ALT value of the Codium fragile group showed a significant decrease in comparison to the negative control group. In summary, this study demonstrated the beneficial possibilities of Codium fragile in improving the abnormality of lipid metabolism caused by liver cell damage and hyperlipidemia.

• 한국자원식물학회지
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• 제19권5호
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• pp.602-605
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• 2006

청각 추출액이 비만을 유도한 흰쥐의 지질강하 및 항산화효과에 미치는 영향을 검토했다. 각 처리군 별 유리지방산의 농도는 청각 처리군 모두가 대조군보다 낮은 값을 보였다. Triglyceride 량은 대조군과 비교하여 청각 처리군이 유의하게 낮은 값을 보였으며, 청각 첨가량이 증가함에 따라 하락했다. 혈액 내 total cholesterol 및 LDL-cholesterol농도는 청각 처리군 모두에서 감소하는 경향을 보였다. 그러나 HDL-cholesterol량은 대조군을 비롯하여 전 처리군에서 유의한 차이를 나타내지 않았다. 간장 내 total cholesterol과 triglyceride량은 청각 처리군 모두에서 하락하는 경향을 보여 정상군의 수준을 유지하였다. 혈장 및 간장의 TBARS 농도는 청각처리군 모두가 하락하는 경향을 보였으며, 정상군의 수준과 유사한 수치를 나타내었다. GSH-Px의 활성치는 청각 처리량이 증가함에 따라 증가하는 경향을 보여, 200mg/kg 처리군에서는 정상군의 활성치와 유의한 차이를 나타내지 않았다. 그러나 SOD 활성치는 청각처리에 관계없이 정상군을 비롯한 전 처리군에서 유의한 차이를 나타내지 않았다. 또한 CAT의 활성치는 청각 처리군에서 다소 증가하는 경향을 보였으나, 대조군과 유의한 차이를 나타내지는 않았다.

• KSBB Journal
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• 제26권4호
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• pp.341-346
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• 2011

This work is focused on the possibility of marine biomass Codium fragile as renewable resources for production of levulinic acid. In an effort to optimize the reaction conditions of levulinic acid production from Codium fragile, response surface methodology was applied. A total of 18 individual experiments were designed to investigate the effect of reaction temperature, catalyst amount, and reaction time. As a result, 4.26 g/L levulinic acid from Codium fragile was produced in the condition of $160.7^{\circ}C$ of reaction temperature, 3.9% of sulfuric acid, and 39.1 min of reaction time. This result will provide the useful information for chemical production from marine resource.

• ALGAE
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• 제31권2호
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• pp.129-136
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• 2016

The Mediterranean Sea is currently facing dramatic changes and threats, including change in native species and accidental introductions. The introduced green alga Codium fragile subsp. fragile (Suringar) Hariot influences diversity and community structure in some parts of the world. This paper documents the distribution of this species in Tunisia and provides a morphological description of C. fragile subsp. fragile in Northern Tunisia. Results confirm the identity of Tunisian specimens as the invasive subspecies C. fragile subsp. fragile. This is the first morphological characterization of this subspecies in Tunisia.

• 대한화학회지
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• 제33권3호
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• pp.321-325
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• 1989

해양 생물에는 육지 생물에 비해 훨씬 다양하고 특이한 구조의 sterol이 나타나고 있으며 이런 특질들은 화학자들에게 여러가지 흥미있는 생화학적 혹은 유기화학적인 관심을 불러 일으며 왔다. 본 연구에서는 한국산 청각(Codium fragile)으로부터 3개의 sterol 성분을 분리 확인하였다. 주 sterol 성분은 탄소가 29인 clerosterol 였으며 codisterol과 cholesterol도 소량 발견되었다.

• International Journal of Oral Biology
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• 제43권2호
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• pp.61-68
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• 2018

Codium fragile (Suringar) Hariot is an edible green seaweed that belong to the Codiaceae family and has been used in Oriental medicine for the treatment of enterobiasis, dropsy, and dysuria. Methanol extract of codium fragile has anti-oxidant, anti-inflammatory and anti-cancer properties, although the anti-cancer effect on oral cancer has not yet been reported. In this study, we investigated the anti-cancer activity and the mechanism of cell death by methanol extracts of Codium fragile (MeCF) on human FaDu hypopharyngeal squamous carcinoma cells. Our data showed that MeCF inhibits cell viability in a dose-dependent manner, and markedly induced apoptosis, as determined by the MTT assay, Live/Dead assay, and DAPI stain. In addition, MeCF induced the proteolytic cleavage of procaspase -3, -7, -9 and poly(ADP-ribose) polymerase(PARP), and upregulated or downregulated the expression of mitochondrial-apoptosis factor, Bax(pro-apoptotic factor), and Bcl-2(anti-apoptotic factor). Futhermore, MeCF induced a cell cycle arrest at the G1/S phase through suppressing the expression of the cell cycle cascade proteins, p21, CDK4, CyclinD1, and phospho-Rb. Taken together, these results indicated that MeCF inhibits cell growth, and this inhibition is mediated by caspase- and mitochondrial-dependent apoptotic pathways through cell cycle arrest at the G1/S phase in human FaDu hypopharyngeal squamous carcinoma cells. Therefore, methanol extracts of Codium fragile can be provided as a novel chemotherapeutic drug due to its growth inhibition effects and induction of apoptosis in human oral cancer cells.

• 문화기술의 융합
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• 제5권4호
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• pp.467-472
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• 2019

해조류는 무기질, 비타민, 단백질, 섬유소가 풍부하며 녹조류에 속하는 청각(Codium fragile)은 청각과이며, 우리나라, 동아시아, 오세아니아 등 해안에 분포해 있고 우리나라와 각국에서 식재료 뿐만 아니라 건강기능소재로 사용되고 있다. 본 연구에서는 청각을 주정으로 추출하고 농축하여 DPPH 라디칼 소거능, SOD 활성, FRAP과 ABTS 양이온 라디칼 소거능을 확인하였다. 그 결과 DPPH 소거능 측정 결과 6.25, 12.50, 25, 50, $100{\mu}g/mL$ 농도에서 0.83, 22.83, 38.27, 40.93, 45.60%의 소거능을 나타냈으며, SOD 유사활성 측정 결과 23.13, 33.63, 33.93, 44.07, 59.07%의 유사활성능을 보였다. FRAP과 ABTS도 앞에서 했던 실험 결과와 마찬가지로 농도의존적으로 항산화 활성을 보였다. 따라서 본 연구결과에서는 천연 소재인 청각을 이용하여 화장품 소재로서 활용할 수 있음을 확인하였다.

• 한국수산과학회지
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• 제53권6호
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• pp.816-822
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• 2020

This study investigated the antioxidant activity and general and sensory properties of soybean curd supplemented with Codium fragile. The yield of soybean curd containing 0%, 20%, and 40% C. fragile was 113.62, 104.09, and 108.06, respectively. Antioxidant activities and pH were significantly increased (P<0.05) depending on the amount of C. fragile supplemented in soybean curd. Compared to that in additive-free soybean curd (control), the amount of crude ash and protein in soybean curd containing 40% C. fragile was significantly decreased (P<0.05) and increased (P<0.05), respectively. We observed a dose-dependent increase (P<0.05) in the hardness and chewiness of soybean curd concomitant with the amount of C. fragile supplemented. In contrast, we observed no significant difference (P>0.05) in soybean curd's cohesiveness between the groups. As determined by sensory evaluation based on seven-point hedonic scale, soybean curd supplemented with 20% C. fragile received an excellent score (6.55) for color. As the same method, we observed that the flavor and overall acceptability significantly increased (P<0.05) in the C. fragile content increased. Taken together, the antioxidant and sensory assays in our study make a compelling case for the practical development of soybean curd supplemented with C. fragile owing to its good antioxidant activities, general properties, and consumer acceptance.

• 생명과학회지
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• 제16권5호
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• pp.788-793
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• 2006

청각은 녹조류로서 김치의 부재료로 널리 사용되는 것으로 청각 추출물이 젖산균의 생육 및 cellulase 활성에 미치는 영향과 항산화 및 면역기능에 미치는 영향을 조사하였다. 청각추출물이 젖산균의 생육에 미치는 영향은 김치의 중기이후 젖산 생성에 중요한 Lactobacillus plantarum KCTC 3105의 생육을 2배정도 촉진하였으며 과숙 김치의 경도유지에 중요한 cellulase의 활성을 약 60% 정도 저해하는 효과를 보였다. 청각추출물의 항산화력은 DPPH 방법이나 SOD 유사활성등에서 비슷한 항산화력을 나타내고 있으며 특히 ethyl acetate 층에서 높은 항산화력을 나타내었는데 이러한 결과는 청각에 존재한 phenolic compound가 관여하는 것으로 생각된다. 청각추출물의 항염증효과는 RAW264.7 세포에서 조사되었는데 특히 치주질환균에서 유도된 LPS를 처리하여 유도된 NO 활성을 현저히 감소시킴으로서 높은 항염증 효과를 나타내었으며 이러한 결과는 iNOS 활성에서도 유사한 결과를 나타내었다.

• 한국수산과학회지
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• 제38권6호
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• pp.393-398
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• 2005

Codium fragile is commercially farmed in Korea by natural blooming zygote attachment. Experiments found optimum conditions for artificial seed production and nursery culture of C. fragile by asexual reproduction. Isolated utricles and medullary filaments were regenerated to erect thalli using both indoor and outdoor culture experiments. Under the indoor culture conditions, irradiance was an important factor to control the development of erect thalli. Formation of erect thallus from the isolated medullary filaments in the indoor culture was induced after 30 days under $20^{\circ}C$ and $60{\mu}mol/m^2/sec$. The detachment of isolated utricles and medullary filaments from the substrates of seed strings was reduced by exposure to the air during 2 hrs before the indoor culture of seed strings. The maximum growth and development of erect thalli in the nursery culture was induced at a water depth of 0.5 m. Depending on the substrates of the seed strings the growth of erect thalli was not significantly different (p>0.05).