Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Studies on Antioxidant Activity and Inhibition of Nitric Oxide Synthesis from Codium fragile

청각추출물의 항산화 및 일산화질소 합성 저해 연구

  • Kim, Yu-Jung (Department of Food and Nutrition, Silla University) ;
  • Jung, Il-Sun (Department of Food and Nutrition, Silla University) ;
  • Choi, In-Soon (Department of Life Science, Silla University) ;
  • Gal, Sang-Wan (Department of Microbiological Engineering, Jinju National University) ;
  • Choi, Young-Ju (Department of Food and Nutrition, Silla University)
  • 김유정 (신라대학교 의생명과학대학 식품영양학과) ;
  • 정일선 (신라대학교 의생명과학대학 식품영양학과) ;
  • 최인순 (신라대학교 의생명과학대학 생명과학과) ;
  • 갈상완 (진주산업대학교 미생물공학과) ;
  • 최영주 (신라대학교 의생명과학대학 식품영양학과)
  • Published : 2006.08.30
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Abstract

This study was carried out to investigate the biological effects from Codium fragile. Methanol extract of Codium fragile increased two times at 2500 ㎍/ml the growth of Lactobacillus plantarum that associated with probiotic properties of lactic acid bacteria of Kimchi. Ethyl acetate extract of Codium fragile inhibited the cellulase activity up to approximately 60% at $2500\;{\mu}g/ml$. Methanol extract of Codium fragile was fractionated into several subfractions and their antioxidant activities were measured by using DPPH radical scavenging and SOD-like activity. Especially the antioxidative activity of ethyl acetate fraction was shown higher than that of other fractions and its fraction showed higher contents of total phenolic compounds, indicating the positive relationship between DPPH radical scavenging effect and total polyphenol content. Stimulation of the macrophages RAW264.7 cells with lipopolysaccharide (LPS) resulted in increased production of nitric oxide (NO) in the medium. However, the methanol extract of Codium fragile showed marked inhibition of NO synthesis in a dose-dependent manner. This result suggest that Codium fragile plays significant role for activation of immune system in the pathogenesis of inflammatory diseases.

청각은 녹조류로서 김치의 부재료로 널리 사용되는 것으로 청각 추출물이 젖산균의 생육 및 cellulase 활성에 미치는 영향과 항산화 및 면역기능에 미치는 영향을 조사하였다. 청각추출물이 젖산균의 생육에 미치는 영향은 김치의 중기이후 젖산 생성에 중요한 Lactobacillus plantarum KCTC 3105의 생육을 2배정도 촉진하였으며 과숙 김치의 경도유지에 중요한 cellulase의 활성을 약 60% 정도 저해하는 효과를 보였다. 청각추출물의 항산화력은 DPPH 방법이나 SOD 유사활성등에서 비슷한 항산화력을 나타내고 있으며 특히 ethyl acetate 층에서 높은 항산화력을 나타내었는데 이러한 결과는 청각에 존재한 phenolic compound가 관여하는 것으로 생각된다. 청각추출물의 항염증효과는 RAW264.7 세포에서 조사되었는데 특히 치주질환균에서 유도된 LPS를 처리하여 유도된 NO 활성을 현저히 감소시킴으로서 높은 항염증 효과를 나타내었으며 이러한 결과는 iNOS 활성에서도 유사한 결과를 나타내었다.

Keywords

References

  1. An, B. J., J. T. Lee, J. H. Kwak, J. M. Park, J. Y. Lee and J. H. Son. 2004. Antioxidant effects and application as natural ingredients of Korean Sanguisorbae officinalis. L. J. Korean Soc. Appl. Biol. Chem. 47, 244-250
  2. An, Jeung Hee. 2001. Studies on complement activating and nitric oxide generation inhibitory phytochemicals from Codium fragile Curcuma heyneana. PhD Dissertation. Korea University
  3. AOAC. 1984. Official Methods of Analysis. 14th ed. Method 9. 110. Association of Official Analytical Communities, Arlington, VA. U.S.A
  4. Arasaki, S. and T. Arasaki. 1983. Vegetables from the Sea. Japan Pub. Tokyo, p. 169-189
  5. Bannister, J. V., W. H. Bannister and G. Rotilio. 1987. Aspects of the structure, function and applications of superoxide dismutase. CRC Crit. Rev. Biochem. 22, 111-180 https://doi.org/10.3109/10409238709083738
  6. Belitz, H. D. and W. Grosch, 1987. Food Chemistry. p.175, Springer-Verlag, Berlin
  7. Blois, M. S. 1958. Antioxidant determination by the use of a stable radical. Nature 26, 1199-1200
  8. Chung, K. S., S.-Y. Kang and J Y. Kim. 2003. The antibacterial activity of garlic juice against pathogenic bacteria and lactic acid bacteria. Korean J. Microbiol. Biotechnol. 31, 32-35
  9. Cho, K. J., Y. S. Lee and B. H. Ryu. 1990. Antitumor effect and immunology activity of seaweeds toward sarcoma- 180. Bull. Kor. Fish. Soc. 23, 345-351
  10. Farias-Eisner, R., M. P. Sherman, E. Aeberhard and G. Chaudhuri 1994. Nitric oxide is an important mediator for tumoricidal activity in vivo. Proc. Natl. Acad. Sci. 91, 9407-9411
  11. Hwang, E. K., J. M. Baek and C. S. Park. 2005. Artficial seed production using the reproduction methods in Codium fragile (Chlorophyta). J. Korean Fish. Soc. 38, 164-171 https://doi.org/10.5657/kfas.2005.38.3.164
  12. Ignarro, L. J., J. M. Fukutto, J. M. Griscavage, N. E. Rogers and R. E. Byrns. 1993. Oxidation of nitric oxide in aqueous solution to nitrite but not nitrite: Comparison with enzymatically formed nitric oxide form L-arginine. Proc. Natl. Acad. Sci. 90, 8103-8107
  13. Jung, K.-J. C. H. Jung, J.-H. Pyeun and Y. J. Choi. 2005. Changes of food components in mesangi (Capsosiphon fulvecense), gashiparae (Enteromorpha prolifera), and cheonggak (Codium frafile) depending on harvest times. J. Korean Soc. Food. Sci. Nutr. 34, 687-693 https://doi.org/10.3746/jkfn.2005.34.5.687
  14. Kim S.-D., M.-H. Kim and D.-H. Kim. 2000. Effect of dandelion (Traxancum Platycarpum D.) extracts on the growth of lactic acid bacteria and gas formation from kimchi. Korean J. Postharvest Sc. Technol. 7, 321-325
  15. Kim, K.-H. 2002. Effect of addition methods of green tea on fermentation characteristics of kimchi. Korean J. Food Preservation 9, 406-410
  16. Kim, Y. C. and S. K. Chung. 2002. Reactive oxygen radical species scavenging effects of Korean medical plant leaves. Food Sci. Biotechnol. 11, 407-411
  17. Kwak, C. S., S. A. Kim and M. S. Lee. 2005. The correlation of antioxidative effects of 5 korean common edible seaweeds and total polyphenol content. J. Korean Soc. Food Sci. Nutr. 34, 1143-1150 https://doi.org/10.3746/jkfn.2005.34.8.1143
  18. Lee, C. W., C. Y. Ko and D. M. Ha, 1992. Microfloral changes of lactic acid bacteria during kimchi fermentation and identification of the isolates. Korean J. Appl. Microbial. 20, 102-238
  19. Lee, S.-H., J.-S. Choi, K.-N. Park, Y.-S. Im and W.J. Choi. 2002. Effects of Prunus mume sie. extract on growth of lactic acid bacteria isolated from kimchi and preservation of kimchi. Korean J. Food Preservation. 9, 292-297
  20. Lu, Y. and L. Y. Foo. 2000. Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chem. 68, 81-85 https://doi.org/10.1016/S0308-8146(99)00167-3
  21. Lin, H.-Y., S.-H. Juan, S.-C. Shen, F. L. Hsu and Y. C. Chen. 2003. Inhibition of lipopolysaccharide-induced nitric oxide production by flavonoids in RAW264.7 macrophages involves heme oxygenase-1. Biochemical Pharmcology 66, 1821-1832 https://doi.org/10.1016/S0006-2952(03)00422-2
  22. Marklund, S. and G. Marklund, 1975. Involvement of superoxide aminoradical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47, 468-474
  23. Marletta, M. A. 1993. Nitric oxide synthase structure and mechanism. J. Biol. Chem. 268, 12231-12234
  24. Miller, G. L., R. Blum, W. E. Glennon and A. L. Burton. 1960. Measurement of carboxymethylcellulase activity. Anal. Biochem. 2, 127-132
  25. Moon, Y.-J., S. Park and C.-K. Sung. 2003. Effect of ethanolic extract of Schizandra chinensis for the delayed ripening kimchi preparation. Korean J. Food & Nutr. 16, 7-14
  26. Na, G. M., H. S. Han, S. H. Ye and H. K. Kim. 2004. Extraction characteristics and antioxidative activity of Cassia tora L. extracts. Korean J. Food Culture 19, 499-505
  27. Nathan, C. 1992. Nitric oxide as a secretary product of mammalian cells. FASEB J. 6, 3051-3064 https://doi.org/10.1096/fasebj.6.12.1381691
  28. Nice D. J., D. S. Robinson and M. A. Jolden. 1995. Characterization of a heat-stable antioxidant co-purified with the superoxide dismutase activity from dried peas. Food Chem. 52, 393-397 https://doi.org/10.1016/0308-8146(95)93288-3
  29. Oh, Y. S., I. K. Lee and S. M. Boo, 1990. An annotated account of Korean economic seaweeds for food, medical and industrial uses. Kor. J. Phycol, 5, 57-71
  30. Park, N. A., H. B.Jo, I. S. Cho, H. J. Kim, S. J. Cho and D. I. Kim, 2001. The analysis of phenolic compounds and vitamin C content in brewed green tea and determination of EDA in catechines(II). Report of S.I.H.E. 37, 98-104
  31. Park, Y. K., W. Y. Lee, S. Y. Park, J. K. Ahn and M. S. Han. 2005. Antioxidant activity and total phenolic content of Callistemon citrinus extracts. Food Sci. Biotechnol. 14, 212-215
  32. Pryor, W. A. 1986. Oxy-radicals and related specied: their formation, lifetimes and reactions. Ann. Rev. Physiol. 48, 657-667 https://doi.org/10.1146/annurev.ph.48.030186.003301
  33. Pyo, Y. H., T. C. Lee, L. Logendra and R. T. Rogen. 2004. Antioxidant activity and phenolic compounds of Swiss chard (Beta vulgaris subspecies cycla) extracts. Food Chem. 85, 19-26 https://doi.org/10.1016/S0308-8146(03)00294-2
  34. Rogers, D. J., K. M. Jurd, G. Blunden, S. Paoletti and F. Zanetti. 1990. Anticoagulant activity of a proteoglycan in extracts of Codium fagile sp. Atlanticum. J. Appl. phycol. 2, 357-361 https://doi.org/10.1007/BF02180926
  35. Rogers, D. J. and R. W. Loveless. 1991. Electron microscopy of human erythrocytes agglutinated by lectin from Codium fragile sp. tomentosoides and pseudohae- magglutinin from Ascophyllum nodosum. J. Appl. Phycol. 3, 83-86 https://doi.org/10.1007/BF00003921
  36. Torel, J., J. Gillard and P. Gillard. 1986. Antioxidant activity of flavonoids and reactivity with peroxy radical. Phytochemistry 25, 383-385 https://doi.org/10.1016/S0031-9422(00)85485-0

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