• 한국유기농업학회지
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• 제24권4호
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• pp.907-917
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• 2016

본 연구에서는 양분 유실이 많고 이용효율이 낮은 유박 전면시비에서 유박 줄뿌림으로 시비방법을 개선하였을 때 유박의 처리 깊이, 위치, 투입량에 따른 옥수수 생육 변화와 양분이용효율 변화를 2년간 조사하였다. 옥수수 생육조사결과, 유박 전면시비와 비교하여 유박 줄뿌림 처리구가 초장, SPAD값, 줄기와 알곡의 건물중 등에서 전반적으로 좋은 생육을 보였으며, 특히 유박 줄뿌림 처리구중에서도 깊이를 5 cm로 옥수수 근권에 줄뿌림 시비하는 것이 가장 효과적인 것으로 나타났다. 양분이용효율 측면에서도 유박 줄뿌림 처리구에서 높은 효율을 보였으며 유박을 옥수수 근권에 줄뿌림 시비로 개선하여 처리한다면 추비시 투입량을 1/2로 줄여도 옥수수 알곡에서 양분이용효율은 감소하지 않는 것으로 나타났다. 유박 근권 줄뿌림 시비방법은 유박의 투입량도 기준방법과 비교하여 25% 줄일 수 있고 양분이용 측면에서 효과적인 방법으로 판단된다.

• 생명과학회지
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• 제8권1호
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• pp.60-66
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• 1998

G- 및 C-banding법에 의한 ICR 생쥐의 유사분열과 감수분열 시기의 염색체 특징에 관하여 조사하였다. 이를 위한 염색체 표본 작성은 Imai et al.의 공기건조법을 다소 변형한 방법에 의하였다. 정소를 이용한 염색체 분석은 유사분열뿐만 아니라 감수분열단계들을 모두 관찰할 수 있었으며, C-banding에 의하여 X 염색체를 포함한 모든 염색체의 돈원체 부위가 강하게 염색되었다. 정상의 제 1 감수분열 전기와 중기 사이의 세포들에서 2가 염색체로 된 19쌍의 상염색체과 1개의 말단결합으로 된X-Y 염색체를 관찰할 수 있었다. 대조군의 제 1 정모세포에서 조기분리된 성염색체를 가지는 세포의 빈도는 약 7.45%였으나,alkylating agents 처리군에서는 대조군에서보다 약 3-4배 이상 높에 나타났다. 그리고 감수분열 단계의 염색체 표본에 C-banding을 적용함으로서 X-Y 염색체 조기분리의 관찰이 매우 용이하였다.

• 한국연초학회지
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• 제19권1호
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• pp.33-39
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• 1997

Recently the method of basal compound fertilizer (N : P : K = 18.2 : 9.8 : 35.0) Placement has been changed from banding to broadcasting in tobacco cultivation. The effects of the compound fertilizer placement, level of fertilizer and additional urea application on the agronomic characteristics, two chemical compositions and physical Properties of burley tobacco(Nicotiana tabacum L. cv. KB 108) were investigated at Chonju Experiment Station, Korea Ginseng and Tobacco Research Institute in 1995-1996. Six treatments consisted of (1) band (method of fertilizer Placement) + In kg/10a(compound fertilizer) + 0(additional urea applied), (2) broadcast+140+0, (3) broadcast+140+25, (4) band+180+0, (5) broadcast+180+0, and (6) broadcast +180+25. The additional urea was applied at hilling. No significant differences were detected between banding and broadcasting method of compound fertilizer Placement in field 9rowth, wield, organoleptic qualify(price Per kilogram), chemical composition and Physical properties of cured leaf, The increased compound fertilizer by 30%(40k/10a) or the additional urea application by 25kg per 10a produced slightly higher yield than the recommended amount of basal compound fertilizer without additional urea application did. It also increased the total alkaloid content of cured leaf. It is recommended that no more basal fertilizer above the recommended amount and no additional urea application are needed in burley tobacco fertilization, even though the method of basal fertilizer placement being chanced from banding to broadcasting. Key words : Nicotiana tabacum, fertilizer placement, additional urea.

• 한국산업보건학회지
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• 제25권1호
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• pp.95-103
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• 2015

Objectives: This study was conducted in order to better understand the conceptual model and Stoffenmanager nano module and apply it to the synthesis and packing processes of nanomaterials. Methods: Site visits were conducted to five nanomaterial production processes. Product and exposure variables were investigated in these workplaces. Hazard banding and exposure classification of the synthesis and packing processes of nanomaterials were conducted using documents and the website of Stoffenmanager Nano. Results: The five sites featured different products, packing tasks, ventilation and local exhaust, and others. The hazards for nano-nickel and copper were classified as E. The hazards for both fumed silica and indium tin oxide were classified as D. The hazard for spherical silica was classified as C. The exposure classes in the synthesis process of nanomaterials ranged from 2 through 4. The exposure classes in the packing process of nanomaterials ranged from 1 through 4. Conclusions: Application of Stoffenmanager nano to the synthesis and packing processes of nanomaterials helped to better understand the control level of the work environment and to suggest appropriate actions. The comparison of each process showed the effect of the production process and handling of solids and ventilation on exposure class.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2005년도 추계학술대회 논문집
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• pp.847-850
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• 2005

In modern Automobile engineer, continuos development technology and improve on manufacture system are very important to the reinforcement of technology and improvement of productivity. In this study make a comparative welding methodology to make automotive trailing arm for change to welding system. A progress of test is durability test and banding fracture test for make collection of data about properties of welding system.

• Plant Biotechnology Reports
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• 제4권3호
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• pp.237-242
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• 2010

Six parent and their 12 gamma ray-induced somatic flower colour mutants of garden rose were characterized to discriminate the mutants from their respective parents and understanding the genetic diversity using Random amplification of polymorphic DNA (RAPD) markers. Out of 20 primers screened, 14 primers yielded completely identical fragments patterns. The other 7 primers gave highly polymorphic banding patterns among the radiomutants. All the cultivars were identified by using only 7 primers. Moreover, individual mutants were also distinguished by unique RAPD marker bands. Based on the presence or absence of the 48 polymorphic bands, the genetic variations within and among the 18 cultivars were measured. Genetic distance between all 18 cultivars varied from 0.40 to 0.91, as revealed by Jaccard's coefficient matrix. A dendrogram was constructed based on the similarity matrix using the Neighbor Joining Tree method showed three main clusters. The present RAPD analysis can be used not only for estimating genetic diversity present in gamma ray-induced mutants but also for correct identification of mutant/new varieties for their legal protection under plant variety rights.

• Plant Resources
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• 제5권3호
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• pp.169-175
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• 2002

The soybean cyst nematode (Heterodera glycines Inchinoe; SCN) is a devastating pest of soybean and is responsible for significant losses in yield. The use of resistant cultivars is the effective method to reduce or eliminate SCN damage. The objective of this research is to identify AFLP markers linked to the SCN resistant genes. Bulked genomic DNA was made from resistant and susceptible genotypes to SCN and a total of 19 primer combinations were used. About 31 fragments were detected per primer combination. The banding patterns were readily distinguished in resistant and susceptible bulked genotypes. Polymorphic fragments were detected between resistant and susceptible bulked genotypes in the primer combination of CGT/GGC, CAG/GTG and CTC/GAG. In primer combinations of CGT/GGC and CAG/GTG, bulked resistant genotype produced a polymorphic bands. However, in primer of CTC/GAG, bulked susceptible genotype produced a polymorphic fragments. Three AFLP markers identified as a polymorphic fragments between bulked genomic DNA were mapped in 85 F2 population. Among them, only two markers, CGT/GGC and CTC/GAG, was linked and was mapped. Broad application of AFLP marker would be possible for improving resistant cultivars to SCN.

• Journal of Veterinary Science
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• 제24권6호
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• pp.79.1-79.16
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• 2023

Background: The need for a storage method capable of preserving the intrinsic properties of bones without using toxic substances has always been raised. Supercooling is a relatively recently introduced preservation method that meets this need. Supercooling refers to the phenomenon of liquid in which the temperature drops below its freezing point without solidifying or crystallizing. Objectives: The purpose of this study was to identify the preservation efficiency and applicability of the supercooling technique as a cortical bone allograft storage modality. Methods: The biomechanical effects of various storage methods, including deep freezing, cryopreservation, lyophilization, glycerol preservation, and supercooling, were evaluated with the three-point banding test, axial compression test, and electron microscopy. Additionally, cortical bone allografts were applied to the radial bone defect in New Zealand White rabbits to determine the biological effects. The degree of bone union was assessed with postoperative clinical signs, radiography, micro-computed tomography, and biomechanical analysis. Results: The biomechanical properties of cortical bone grafts preserved using glycerol and supercooling method were found to be comparable to those of normal bone while also significantly stronger than deep-frozen, cryopreserved, and lyophilized bone grafts. Preclinical research performed in rabbit radial defect models revealed that supercooled and glycerol-preserved bone allografts exhibited significantly better bone union than other groups. Conclusions: Considering the biomechanical and biological superiority, the supercooling technique could be one of the optimal preservation methods for cortical bone allografts. This study will form the basis for a novel application of supercooling as a bone material preservation technique.

• 식물병연구
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• 제26권3호
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• pp.170-178
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• 2020

바이러스의 정확한 진단법 확립은 바이러스의 피해 및 확산을 예방하는데 매우 중요하게 작용한다. 대부분의 딸기 바이러스는 조직내에 낮은 역가로 분포하여 진단이 어렵고, 특히 딸기 조직은 다당류 및 페놀화합물의 함유가 많아 RNA 추출이 어려운 것으로 알려져 있다. 딸기 우량묘 생산에 필요한 바이러스 검정기술을 확립하기 위해 본 연구에서는 딸기 잎에서 바이러스 진단을 위해 가장 최적의 RNA 추출방법 정립을 위해 다양한 상용 키트와 시약을 이용하여 RNA 추출효율 비교하였다. 바이러스 진단을 통한 RNA 추출효율을 분석하기 위해 SMoV 감염주인 미홍 딸기 품종을 이용하여 다양한 단계에서 잎조직으로부터 RNA를 추출하고 바이러스 진단을 수행하였다. 식물 RNA 추출 방법 가운데 상업용으로 판매되는 RNeasy plant mini kit (Qiagen)를 이용하는 경우 본 연구에서 살펴본 one-step 또는 two-step RT-PCR 방법과 무관하게 SMoV의 검출이 잘 되었다. 또한, 딸기 우량묘의 바이러스 검정에 대한 신뢰있는 진단방법을 구축하기 위해 주요 딸기 바이러스인 strawberry mild yellow edge virus (SMYEV), strawberry mottle virus (SMoV), strawberry latent ringspot virus (SLRSV), strawberry crinkle virus (SCV), strawberry pallidosis associated virus (SPaV), strawberry vein banding virus (SVBV) 및 strawberry necrotic shock virus (SNSV) 7종에 대한 유전자 합성을 통해 진단클론을 제작하였다. 각 클론의 합성유전자를 기반으로 7종의 딸기바이러스 프라이머 세트를 설계하고 편리한 진단법 수행을 위해 동일한 PCR 조건을 설정하였다.

• 대한치과교정학회지
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• 제24권3호
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• pp.659-672
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• 1994

Dental caries is one of the most prevalent dental diseases in Korea and its prevention is very important in orthodontic therapy. For the cleansing action of saliva itself and/or tooth-brushing is lowered in patient with fixed orthodontic appliance, oral hygiene of the patient becomes worse, which provides more favorable environment for micro-organisms. Chlorhexidine, one of the series of bisguanide, has been reported to be strong antimicrobial agent and very effective on Streptococcus mutans. The purpose of this study is to evaluate the possibility of chlorhexidine as a anticariogenic agent in fixed orthodontic therapy. We used the varnish containing chlorhexidine as a main ingredient for the chemical control of salivary S.mutans in patients with fixed appliance therapy We applied the varnish containing chlorhexidine on the labial and interproximal surface of the teeth before bonding and banding teeth of our patients(N=20) and compared to control group patients(N=20). Before the application of chlorhexidine varnish and four times periodically after the completion of fixed appliance set-up, we sampled saliva of both group patients and incuvate S.mutans for 24 hours. In the culture study of sampled saliva, counting the number of S.mutans colonies, we founded as follows : 1. In patients with fixed appliance therapy, the risk of dental caries increase when it compared to that of preorthodontic treatment ; The number of salivary S.mutans increase in Patient's oral cavity. 2. The experimental agent that contain chlorhexidine is effective to reduce the number of salivary S.mutans. 3. For the effect of this agent is not ever-lasting, periodical application is needed, and additional study for economical interval and number of application is needed.