• Title/Summary/Keyword: bacteriological examination

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Clinical and bacteriological studies on infectious atrophic rhinitis of swine (돼지 전염성 위축성 비염의 임상학적 및 세균학적 연구)

  • Kim, Bong-hwan;Tak, Ryun-bin;Cho, Gil-jae;Jang, Hee-kyung
    • Korean Journal of Veterinary Research
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    • v.31 no.4
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    • pp.457-469
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    • 1991
  • Clinical and bacteriological observations on infectious atrophic rhinitis of swine were conducted in order to obtain some basic information for the clinical and immnnological control of the disease prevailing in the republic. Samples were collected from nasal cavities of 135 4~12 week old pigs from 12 herds and from turbinates of 199 slaughtering pigs from 14 swine herds to investigate the prevalence of Bordetella bronchiseptica and/or Pasteurella multocida in the nasal cavities of the pigs. On the examination of nasal swabs by cultural techniques and of gross lesion of snouts of slaughtering pigs, all the swine herds investigated were found to be affected by atrophic rhinitis and a total of 84 B bronchiseptica and 139 P multocida cultures were isolated from the nasal cavities of the pigs. Of the 199 slaughtering pigs, some 48% revealed gross pathological lesion typical of atrophic rhinitis and the prevalence of B bronchiseptica and P multocida were 27.6% and 46.7%, respectively. Biochemical properties, antimicrobial susceptibilities, serological characteristics and toxigenicity of the isolates of B bronchiseptica and P multocida were investigated.

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A Survey on Health Status of Group Controlled Pulmonary Tuberculosis Patients -Application of Cornell Medical Index- (집단관리 결핵환자들의 건강실태조사 -코오넬 의학지수의 응용-)

  • Jung, K.J.
    • Journal of Preventive Medicine and Public Health
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    • v.11 no.1
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    • pp.14-23
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    • 1978
  • This survey was conducted on a total 672 pulmonary tuberculosis patients who were registered at certain health center in Busan, during the period from July 15th to August 31st, 1977, based on Modified Cornell Medical Index(CMI) consisting of 70 questions. Number of 'Yes' response of an individual patients was collected by each large section of Modified CMI. The each number of 'Yes' response was standardized by mean of Z scoring. Z score was obtained by following formula. Z=50+10(Xi-m)/s M : means of 'Yes' response by each section for all subjects s : standard deviation of the mean Xi : number of 'Yes' response by each section in an individual patients The results of obtained were as follows: 1. The number of investigated cases were 672 (459 males and 213 females). The most prevalent group was 20-24 years old group as 18.4% by age, moderate advanced group as 50.8% by radiological diagnosis, INH+PAS+SM group as 34.7% by antituberculotics and unemployed group as 59.9% by occupation. By bacteriological examination of sputum, the rate of negative group was 60.5% and positive group was 39.5%. 2. Z score of complaints by sex was higher in female as 52.4 than in male as 48.9 in general. By radiological diagnosis, there was decreasing tendency with age in male but increasing tendency with age in female. 3. By age group, Z score of complaints was increasing tendency with age in male but there was non-significant differences in female. 4. By bacteriological examination of sputum, the Z score of complaints was increasing tendency with the more discharged bacteria in both sex generally. 5. By antituberculotics, INH group was revealed the highest Z score of complaints as 50.4 in male and INH+PAS group was the highest as 51.21 in female. 6. By occupation, agricultural and fisherman group was the highest as 53.5 and the next group was professional, technical and related workers, unemployed and sales workers in that order.

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Field Study on the Mycotoxin Binding Effects of Clay in Oreochromis niloticus Feeds and Their Impacts on the Performance as Well as the Health Status throughout the Culture Season

  • Abdelaziz, Mohamed;Anwer, Wael;Abdelrazek, Abeer Hamada
    • Interdisciplinary Bio Central
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    • v.2 no.4
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    • pp.10.1-10.5
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    • 2010
  • Total aflatoxin and ochratoxin were detected in 3 naturally contaminated fish feed samples using immune-affinity method. The results revealed that the average levels of aflatoxins in the 3 examined samples were (15, 22 and $12\;{\mu}g/kg$) respectively while the average levels of ochratoxins were (15, 6 and $6\;{\mu}g/kg$). The results of determination of the effects of clay as a mycotoxin binder on the health status and performance of Oreochromis niloticus in comparing with a control group revealed that the survival rate in control group was 81% after the end of the culture season. The results also revealed that the survival rate in group 2 which received clay treated feed was 86%. The results of regular parasitological examination revealed the identification of trichodina as external protozoa in Oreochromis niloticus from both ponds but without manifestation of disease signs. The results of bacteriological examination revealed the isolation and identification of Pseudomonas flouresence from some moribund Oreochromis niloticus. Higher performance parameters were recorded in group 2 that received feeds treated with clay which reflected in the total production which reaches 1646.47 kg while in the control pond, the total production was1308.36 kg.

Selective non digestion of yellow mealworm Tenebrio molitor larvae by arowana

  • Gomez, Dennis Kaw;Kim, Ji Hyung;Choresca, Casiano Hermopia Jr.;Baeck, Gun Wook;Park, Se Chang
    • Korean Journal of Veterinary Research
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    • v.47 no.2
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    • pp.191-195
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    • 2007
  • This study reveals the unusual case reported for the first time on the selective non digestion of yellow mealworm (Tenebrio molitor) larvae by arowana fish. In January 2005, an Asian arowana (Scleropages formosus) (red variety), from Daesang Tropical Fish Corporation, Seoul, Korea, mortality was observed due to unknown cause. No putative causal factors were suggested by bacteriological and parasitological examinations. Internal examination of the dissected stomach showed some undigested debris with mandible parts of mealworms attached to the mucosal lining of stomach wall. Feeding experiment of yellow mealworm (Tenebrio molitor) was conducted on the silver arowana (Osteoglossum bicirrhosum). Result showed that on the test group, fish released vomitous material containing undigested mandibles of mealworm was observed on the 24th day of the feeding experiment. Histopathological examination of the cross section of the stomach wall layers of the test groups, showed detached parts of the mucosal layer and gastric pits around the damaged area with intact mucularis tissues. While the control group fed with mealworm larvae without head part showed intact stomach wall layers consisting of gastric pit, mucosa and mucularis tissues. Fish on both treatments survived until the termination of experiment. The removal of mealworm larvae head before feeding probably help or aid in the fast digestion of these insect larvae.

Evaluation of the Bacteriological Safety for the Shellfish Growing Area in Hansan.Geojeman, Korea (한산.거제만해역 패류양식장에 대한 세균학적 위생안전성 평가)

  • Ha, Kwang-Soo;Shim, Kil-Bo;Yoo, Hyun-Duk;Kim, Ji-Hoe;Lee, Tae-Seek
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.5
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    • pp.449-455
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    • 2009
  • In Hansan Geojeman area, 2,050 ha of shellfish growing area has been designated as shellfish production area for export. The main shellfish species from the designated area is oysters. For the sanitary management of the designated area established in Hansan Geojeman area, bacteriological examination of sea water and shellfish at the sampling stations inside and outside of the designated area were performed from January 2006 to December 2008. The range of fecal coliform of 756 sea water samples at 21 stations located in the designated area were <1.8~>1,600 MPN/100mL. And the range of geometric mean and the estimated 90th percentile of fecal coliform were 1.8~2.9 and 2.7~15.8 MPN/100mL, respectively. Sanitary conditions of the current designated area in Hansan Geojeman meets the required standards of the Fisheries Product Quality Control and National Shellfish Sanitation Program (NSSP, USA) criteria for the approved area. Also, the sanitary status of the shellfish harvested from the designated area met the Korean Shellfish Sanitation Program (KSSP) fecal coliform criterion (<230 MPN/100g). And the human pathogen such as Salmonella spp. and Shigella spp. were not detected from the examined shellfish samples.

A Study on the Effects of Livestock Wastewater on Stream Pollution(with Bacteriological Examination) (축산폐수가 하천오염에 미치는 영향에 관한 연구 (하) -세균학적 조사를 중심으로-)

  • Ryu J. K.;Lee H. S.;Lee W. C.;Kim T. J.;Lee T. J.
    • Journal of the korean veterinary medical association
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    • v.22 no.4
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    • pp.238-245
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    • 1986
  • To evaluate effects of livestock wastewater on stream pollution, the survey on the environmental sanitation, hydrology, water quality, Pollutant loads and bacterial indicators in a stream receiving livestock wastewater discharged from Chun Sung livestock

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Identification of the Aerobic Bacteria Isolated from Kimchi and Laver (김치 및 김에서 분리(分離)한 호기성(好氣性) 세균(細菌)의 동정(同定)에 관(關)하여)

  • Kim, Ho-Sik;Jeong, Yun-Su
    • Applied Biological Chemistry
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    • v.3
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    • pp.19-24
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    • 1962
  • The results of re-examination for the isolates D-28, D-28a from Kimchi and 88-3 from laver are summarized as follows: 1). All these isolates were proved to be Gram positive, catalase positive and spore-forming. 2). While these strains were not consistent with Bacillus megaterium group with respect to Voges-Proskauer reaction and methyl red test, their cultural characteristics were well consistent with this group. 3). These isolates showed the similar suger-fermentability as B. megaterium group with a slight difference. 4). It is proved that, according to the forgoing results and vitamin $B_{12}$ producibility, the isolate 88-3 is identical with B. megaterium NRRL-B 938 and D-28, D-28a, with B. megaterium 425-1 and 425-2, respectively.

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Nontuberculous Pulmonary Infection in Two Patients with Mycobacterium avium-intracellulare Complex and a Patient with M. fortuitum (Mycobacterium Avium-intracellulare Complex와 M. Fortuitum에 의한 폐항산균증(肺抗酸菌症) 3례(例))

  • Kim, S.J.;Hong, Y.P.;Bai, G.H.;Kim, S.C.;Jin, B.W.;Chung, C.M.
    • The Journal of the Korean Society for Microbiology
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    • v.17 no.1
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    • pp.87-93
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    • 1982
  • Two cases of pulmonary disease in a 54 year-old female and a 70 year-old male patient due to Mycobacterium avium-intracellulare complex(MAIC) and a case of pulmonary infection ina 69 year-old male patient due to M. fortuitum(MF) were found recently in this institute. All three patients had a long history of anti-tuberculous chemotherapy because they were initially diagnosed as pulmonary tuberculosis. A 70 year-old male patient infected with MAIC had an unsuccessful chemotherapy history of isoniazid(INH), para-aminosalicylic acid(PAS) and streptomycin(SM) with an incomplete, temporary, symptomatic improvement, for three years since 1964 when he was first diagnosed as pulmonary tuberculosis on physical examination. A 54 year-old female patient infected with MAIC also had an unsuccessful chemotherapy history with the various anti-tuberculous drugs since 1958. Both patients discharged large number of MAIC in their sputum specimens for at least more than one year, but no M. tuberculosis at all. A 69 year-old male patient infected with MF was diagnosed as moderately advanced pulmonary tuberculsis in 1977. Combined chemotherapy with INH+PAS+pyrazinamide(PZA) improved his clinical symptoms, however, his chest radiograph was deteriorated again in 1980 one year after he stopped therapy. Therefore he started chemotherapy again with INH+ethionamide(TH)+cycloserine(CS) but no improvement was noticed. MF was cultured from his sputum in August 1981 and he continuously discharged the same bacilli until last examination of January 1982. Whether all three patients were initially !infected with nontuberculous mycobacteria or complicated with predisposing tuberculosis was not clear because there were no reliable bacteriological examination records.

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Mortality of the Horned Turban Shell, Batillus cornutus Caused by Vibrio spp (소라(Batillus cornutus)의 비브리오균 감염에 의한 폐사)

  • 이정재;허문수
    • The Korean Journal of Malacology
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    • v.15 no.1
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    • pp.49-55
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    • 1999
  • Mass mortality of the horned turban shell, Batillus cornutus was investigated using histological and bacteriological methods. Some pathogenic bacteria were isolated from mortal or inactive individuals. The pathogenic agents causing mortality of the horned turban shells were as Vibrio alginolyticus and V. anguillarum. Laboratory experiment indicated that optimal growth temperature of two bacteria was 25 to 30$^{\circ}C$ and 3% of NaCl. Histological examination of the horned turban shells showed that gill necrosis is one of the major symptom of infected individuals. It was believed that sudden increase of those two bacterial agents due to environmental change cause mortality of horned turban shells.

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Epidemiological Investigation of an Outbreak of Salmonellosis in Gyeongju, Korea

  • Yoo, Seok-Ju;Lim, Hyun-Sul;Lee, Kwan
    • Journal of Preventive Medicine and Public Health
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    • v.47 no.3
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    • pp.177-181
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    • 2014
  • Objectives: A salmonellosis outbreak occurred within a community of Gyeongju residents who ingested catered food from a wedding in June 2009. We aimed to epidemiologically investigate the probable vehicle of the infection. Methods: We conducted a retrospective cohort study on 34 local residents who ingested the wedding food. Results: Among the 34 residents, 31 (91.2%) reported symptoms of infection after eating the food. Among all of the wedding foods, pan-fried foods were highly associated with the diarrheal attack rate. On bacteriological examination, Salmonella species were detected in the pan-fried foods among the leftover foods and in 17 of the 31 stool specimens from the cases. There were five different types of pan-fried foods, but the onset of symptoms was independent of the ingredients used. We found that the pan-fried food was prepared at a food store in Seoul and that eggs were a common ingredient. Conclusions: The major cause of the salmonellosis in this population was presumed to be the pan-fried food prepared with contaminated eggs. These food items might have been partially undercooked because of their irregular shape, which allowed the Salmonella species to survive and multiply before ingestion.