• Title/Summary/Keyword: bacterial leaf spot

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Evaluation of Antimicrobial Activity and Disease Control Efficacy of Sodium Dichloroisocyanurate (NaDCC) Against Major Strawberry Diseases (딸기 주요 병원균에 대한 친환경제제 NaDCC의 항균활성 및 병 방제효과 평가)

  • Kim, Da-Ran;Gang, Gun-Hye;Cho, Hyun-Ji;Yoon, Hae-Suk;Kwak, Youn-Sig
    • The Korean Journal of Pesticide Science
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    • v.19 no.1
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    • pp.47-53
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    • 2015
  • Various diseases occur in fruits, leave and roots during strawberry cultivation and cause severe economical damage and huge amount of chemical fungicide use. Recently, as consumers' interest in safety of foods and organic agriculture produces have increased, control measures using alternatives for chemical fungicides have been newly developed in various ways. This study was conducted to test antifungal activity and control effect of sodium dichloroisocyanurate (NaDCC), using as disinfectants, against major disease pathogens of strawberry, Fusarium oxysporum (Fusarium wilt), Colletotrichum gloeosporioides (Anthracnose) and Phytophthora sp. (Phytophthora blight), and Xanthomonas fragariae (bacterial angular leaf spot) and evaluate availability as environment-friendly materials. When NaDCC was treated at the concentration range of 150 to 300 ppm, it suppressed significantly hyphal growth and reduced spore germination by more than 28%. In field condition, NaDCC showed excellent control effect (control value: 50%) against the bacterial angular leaf spot disease. Based on above-described results, we suggested that NaDCC can be used as alternative candidates to chemical pesticide alternatives of for controlling strawberry diseases.

Halo Blight of Kudzu Vine Caused by Pseudomonas syringae pv. phaseolicola in Korea

  • Jeon, Yong-Ho;Chang, Sung-Pae;Kim, Sang-Gyu;Kim, Young-Ho
    • The Plant Pathology Journal
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    • v.22 no.2
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    • pp.119-124
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    • 2006
  • Kudzu vine(Pueraria montana var. lobata) is an invasive climbing woody vine that envelops trees and shrubs, pressing physically and shutting out sunlight, which needs to be controlled. Kudzu vine pathogens were surveyed as a way to seek its biocontrol agents in 2002. Occurrence of a bacterial halo blight disease of kudzu vine was observed at several localities in Korea including Euiwang and Suwon in Gyeonggi Province, Daejon, and Gochang and Buan in Jeonbuk Province. Symptoms of brown to black spots with a surrounding yellowish halo appeared from June and lasted till the rainy season without much expansion, but accompanying often leaf blight and defoliation. Isolated bacteria were identified as Pseudomonas syringae pv. phaseolicola based on physiological and cultural characteristics, Biolog, fatty acid and 16S rDNA sequencing analyses. In artificial inoculation test, these bacteria produced the same halo spot symptoms on kudzu vine and bean plants. They also induced hypersensitive responses (HR) on tobacco, tomato, and chili pepper leaves. This is the first report of a bacterial disease of kudzu vine in Korea, and the bacterial pathogen can be used as a biocontrol agent against the pest plant.

Review of Disease Incidence of Major Crops in 2003 (2003년 농작물 병해 발생개황)

  • Kim, Choong-Hoe
    • Research in Plant Disease
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    • v.10 no.1
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    • pp.1-7
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    • 2004
  • The year of 2003 was characterized as a cool humid year. Low temperature and frequent rains were continued during March to July, resulting in 1.6 times higher rainfalls and 32% less sunshine period compared to the average yean Due to 2003's climatic condition, rice blast, and bacterial leaf blight occurred severely. Higher rainfalls caused severe epidemic of phytophthora disease and, in case of red-pepper, 55% of cultivation acreage was devastated by the disease over the country. Besides, crop diseases which become severe under cool-humid conditions, such as gray mold, sclerotinia rot, downy mildew, increased significantly compared to the previous year. In fruit trees, brown spot of apple, and pear scab occurred severely causing much yield loss.

Graft Transmission and Cytopathology of Pear Black Necrotic Leaf Spot (PBNLS) Disease

  • Nam, Ki-Woong;Kim, Kyung-Soo
    • The Plant Pathology Journal
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    • v.18 no.6
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    • pp.301-307
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    • 2002
  • Graft transmission and cytopathological studies of a severe pear disease, pear black necrotic leafspot(PBNLS), were carried out to determine the causal agent of the disease. No evidence was found that a fungal or bacterial pathogen could be the causal agent of the disease. Attempts to transmit the agent by sap-inoculation to other plants including herbaceous hosts failed. How-ever, the pathogen was readily graft-transmitted from symptomatic diseased pears to healthy pears. Graft transmission of the pathogen was also demonstrated by using an indicator plant, PS-95, developed in the laboratory through various grafting methods. Ultrastructural study of the disease revealed the consistent presence of flexuous rod-shaped virus-like particles (VLP) in the symptomatic leaves of both Niitaka cultivar and indicator pear, PS-95. The particles, approximately 12 nm in diameter with undetermined length, occurred in the cytoplasm of mesophyll parenchyma cells. Cells with VLPs also contained fibril-containing vesicles, which are common in cells infected with plant viruses with ssRNA genome. The vesicles were formed at the tonoplast. Based on the symptomatology, the presence of fibril-containing vesicles, and graft-transmissibility, it is believed that the VLPs that occurred on symptomatic leaves of black necrotic leafspot of pear are viral in nature, possibly those of a capillovirus.

Antibacterial Activities against Plant Pathogens and Identification of Agrimol B from Agrimonia pilosa LEDEB (식물병원균에 대한 짚신나물 (선학초) 추출물의 항균활성과 Agrimol B의 동정)

  • Chun, Sung-Bong;Yang, Ba-Rom;Choi, Chun-Whan;Kim, Ik-Soo;Park, Kyung-Seok
    • The Korean Journal of Pesticide Science
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    • v.10 no.3
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    • pp.230-236
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    • 2006
  • Eighty-five percent methanol extract of Agrimonia pilosa has antibacterial activity against Pseudomonas syringae pv. lacrymans (bacterial leaf spot pathogen), Ralstonia solanacearum (tomato bacterial wilt pathogen) and Pseudomonas syringae pv. tabaci (Tobacco wild fire pathogen.). The active substance was purified by silica gel column chromatography and HPLC. The molecular weight of the active compound was determined by LC-Mass as 687.2. With NMR analysis, the active substance was identified as Agrimol B.

A Three-Year Field Validation Study to Improve the Integrated Pest Management of Hot Pepper

  • Kim, Ji-Hoon;Yun, Sung-Chul
    • The Plant Pathology Journal
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    • v.29 no.3
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    • pp.294-304
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    • 2013
  • To improve the integrated pest management (IPM) of hot pepper, field study was conducted in Hwasung from 2010 to 2012 and an IPM system was developed to help growers decide when to apply pesticides to control anthracnose, tobacco budworm, Phytophthora blight, bacterial wilt, and bacterial leaf spot. The three field treatments consisted of IPM sprays following the forecast model advisory, a periodic spray at 7-to-10-day intervals, and no spray (control). The number of annual pesticide applications for the IPM treatment ranged from six to eight, whereas the plots subjected to the periodic treatment received pesticide 11 or 12 times annually for three years. Compared to the former strategy, our improved IPM strategy features more intense pest management, with frequent spraying for anthracnose and mixed spraying for tobacco budworm or Phytophthora blight. The incidences for no pesticide control in 2010, 2011, and 2012 were 91, 97.6, and 41.4%, respectively. Conversely, the incidences for the IPM treatment for those years were 7.6, 62.6, and 2%, and the yields from IPM-treated plots were 48.6 kg, 12.1 kg, and 48.8 kg. The incidence and yield in the IPM-treated plots were almost the same as those of the periodic treatment except in 2011, in which no unnecessary sprays were given, meaning that the IPM control was quite successful. From reviewing eight years of field work, sophisticated forecasts that optimize pesticide spray timing reveal that reliance on pesticides can be reduced without compromising yield. Eco-friendly strategies can be implemented in the pest management of hot pepper.

Occurrences of Major Diseases and Pests on 'Goldone', 'Redvita', 'Garmrok', New Cultivars of Kiwifruit (참다래 신품종 '골드원', '레드비타', '감록'의 주요 병해충 발생)

  • Kim, Min-Jung;Chae, Dae-han;Kwon, Youngho;Kwack, Yong-Bum;Kwak, Youn-Sig
    • Research in Plant Disease
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    • v.24 no.2
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    • pp.123-131
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    • 2018
  • Kiwifruit has been cultivated in southern coastal regions of Korea since late 1970s. New cultivars have been successively released in recent years. In this study, we investigated major disease and pest incidences in new kiwifruit cultivars 'Goldone', 'Redvita' and 'Garmrok' at open field in Sacheon for 3 years and rain-proof field in Jeju for 2 years. For the bacterial canker, the 3 new cultivars showed more disease occurrence in Sacheon but rare in Jeju. For leaf spot disease, compared to disease incidence of 20% on 'Hayward' in Sacheon, cv. 'Garmrok' had high incidence about 60% but cv. 'Goldone' and cv. 'Redvita' had low incidence less than 20%. However, in Jeju, diseases incidences of all the new cultivars were lesser than 20%. In the case of Hemiptera, many Halyomorpha halys and Nezara antennata appeared in Sacheon but in Jeju Plautia stali was dominated. Isolated bacterial canker pathogen was identified as Pseudomonas syringae pv. actinidiae biovar 3. Leaf spots pathogens were Phomopsis sp., Phoma sp., Fusarium tricinctum and Alternaria alternata. This study shows the disease information on new kiwifruit cultivars and the adequate disease managements will be required.

Controlling activity of Bion-M against bacterial spot caused by Xanthomonas euvesicatoria (고추 세균점무늬병에 대한 Bion-M의 방제 효과)

  • Kim, A-Hyeong;Yeon, Cho-Long;Kim, Joo-Hyung;Kim, Heung-Tae
    • The Korean Journal of Pesticide Science
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    • v.16 no.2
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    • pp.171-177
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    • 2012
  • Pepper seedlings of 6-leaf stage were inoculated with cell suspension of Xanthomonas euvesicatoria 1523 7 days after the application of Bion-M by soil-drenching. Disease severity in the treatment with 20 ${\mu}g\;mL^{-1}$ of acibenzolar-S-methyl, with which Bion-M was composed, was 19%, whereas that of the untreated control was 75%. The resulting control value of acibenzolar-S-methyl was calaulated as much as 74.7%. The control value of acibenzolar-S-methyl was dependent with the applied concentrations and ranged 29.3% to 49.3% at 4.0 and 0.8 ${\mu}g\;mL^{-1}$ of acibenzolar-S-methyl, respectively. Phytotoxicity was observed at 20.0 ${\mu}g\;mL^{-1}$, as lower leaves became to be yellowed and defoliated. The cell density of inoculum suspension of X. euvesicatoria 1523 affected the control value of acibenzolar-S-methyl. With optical density (O.D.) of 0.5 the control value of 4.0 ${\mu}g\;mL^{-1}$ of acibenzolar-S-methyl was 86.0%. However, the control value improved as high as 97.8% at the O.D. value of 0.1. The control value was 75.0% in adult plant of pepper, when acibenzolar-S-methyl was treated by soil-drenching 7 days before inoculation with cell suspension of X. euvesicatoria 1523. The control effect of acibenzolar-S-methyl on pepper bacterial spot was obtained in pepper field, showing that the control value at 10.0 ${\mu}g\;mL^{-1}$ was 71.2%.

Review of Disease Incidence of Major Crops in 2001 (2001년 농작물 병해 발생개황)

  • Kim, Choong-Hoe
    • Research in Plant Disease
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    • v.8 no.1
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    • pp.1-10
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    • 2002
  • Climate in the year of 2001 could be summarized as severe drought from March to May, unusually high temperature in whole plant growth periods and clear weather condition especially in harvesting time of September and October without any typoons. In rice, major diseases such as leaf and panicle blast, bacterial blight, sheath blight and bacterial grain rot developed little due to unfavorable weather conditions, however, brown spot occurred severely due partly to the reduced use of N-fertilizer, Rice stripe virus infection was unusually high mainly in the west coast areas with four times more diseaseD area campared to the previous year, In pepper phytophthora blight was extremely severe in Cheonnam and Cheonbuk provinces, where had frequent rainfalls during growing period. Incidence of major diseases of tomato and cucumber in 2001 was relatively mild. In watermelon, penicillium fruit rot, one of the peculiar disease, spread over major production areas, whereas CGMMV, usually severe in every years was much reduced. Watermelon plants growing in open-fields were more severely diseased than those in plastic houses. Major diseases of chinese melon and strawberry occurred slightly and in particular, sudden wilt syndrome of chinese melon which was severe in 2000 showed mild infections in 2001. Incidence of white rot of garlic and onion was much lower unlike other years due mainly to spring drought and high temperature in the growing period. Infected area of potato bacterial wilt tended to increase in 2001 while fusarium wilt of sweetpotato was decreased. Apple diseases were generally mild, but powdery mildew of pear increased sharply. Barley scab was not severe as seen in other years.

Survey of Major Disease Incidence of Rice in Korea during 1999-2008 (1999-2008년도 벼 주요 병해 발생 개황)

  • Lee, Yong-Hwan;Ra, Dong-Soo;Yeh, Wan-Hae;Choi, Hyo-Won;Myung, Inn-Shik;Lee, Se-Won;Lee, Yeong-Hoon;Han, Sung-Suk;Shim, Hong-Sik
    • Research in Plant Disease
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    • v.16 no.2
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    • pp.183-190
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    • 2010
  • During the period from 1999 to 2008, we surveyed the major diseases incidence of rice in Korea. Leaf blast showed 0.85% of the diseased leaf area in 2000 and then consistently decreased to 0.02% by 2008. However, panicle blast irregularly appeared by the years depending on weather conditions for disease development from heading to milk stage of rice. Diseased lesion height of sheath blight had the lowest (10.1%) incidence in 2001 and the highest (21.4%) incidence in 2007. A negative corelation ($r=-0.88^*$) was measured between diseased lesion height of sheat blight and sunshine hours from mid- to late August. In Chunbuk, Gyeongbuk, and Gyeongnam provinces, brown spot occurrence declined since 2001, however, in Chungnam, Jeonbuk, and Jeonnam provinces, it increased since 2005. False smut drastically decreased after showing the highest incidence of 7.59% in panicles in 2000. The correlation coefficient between diseased panicles of false smut and rainfall during the late August was calculated as $0.94^{**}$. Bacterial leaf blight severely increased in the chronically infested fields in Jeonnam, Jeonbuk, Gyeongnam, and Chungnam province since 2003.