• 식물병연구
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• 제23권4호
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• pp.295-305
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• 2017

This study aimed to investigate the plant growth-promoting and biocontrol potential of a grass culture with Paenibacillus ehimensis KWN8 on tomato. For this experiment, treatments of a chemical fertilizer (F), a bacterial grass culture (G), a 1/3 volume of G plus 2/3 F (GF), and F plus a synthetic fungicide (FSf) were applied to tomato leaves and roots. The result showed that the severity of Alternaria solani and Botrytis cinerea symptoms were significantly reduced after the application of the bacterial grass culture (G and GF) and FSf. In addition, root mortality in G and GF was lower compared to F. Tomato plants treated with G or GF had better vegetative growth and yield compared to F. Application of G affected the fungal and bacterial populations in the soil. In conclusion, treatment with a bacterial grass culture decreased disease severity and increased tomato growth parameters. However, there were no statistically significant correlations between disease occurrence and tomato yields. This experiment presents the possibility to manage diseases of tomato in an environmentally friendly manner and to also increase the yield of tomato by using a grass culture broth containing P. ehimensis KWN38.

• 미생물학회지
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• 제50권3호
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• pp.227-232
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• 2014

본 연구에서는 bacterial cellulose를 생산하는 감귤에서 분리된 Gluconacetobacter sp. gel_SEA623-2를 이용하여 초기 초산농도 및 Lactobacillus plantarum KCCM 80077 유산균을 접종한 mixed culture가 bacterial cellulose 생산성에 미치는 영향을 알아보았다. 0.5%의 초산을 첨가한 mixed culture의 경우 초기 생균수가 $2.4{\times}10^6CFU/ml$에서 14일 동안 배양한 이후에 $1.1{\times}10^7CFU/ml$까지 증가하였고 total acidity가 0.5%의 초산을 첨가한 single culture와 비교하여 0.3% 가량 높게 측정되어 유산균에 의해 추가적으로 젖산이 생성된 것으로 판단된다. Single culture에서는 초기 초산농도가 0.0 및 0.5%일 때 1.0% 농도와 비교하여 상대적으로 높은 bacterial cellulose 생산성을 나타내기는 하였으나 유의적인 차이는 보이지 못하였다. 하지만 mixed culture에서는 0.5%의 초산을 첨가하였을 때 $37.83{\pm}6.81g/L$의 dry weight과 $10.33{\pm}0.58mm$의 thickness를 나타냄으로써 1%의 초산을 첨가한 single culture의 $28.40{\pm}1.23g/L$와 $7.50{\pm}0.50mm$의 생산량과 비교하여 유의적인 차이를 나타내었다(P<0.05).

• 한국식품영양과학회지
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• 제31권4호
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• pp.572-577
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• 2002

Gluconacetobacter persimmonus KJ145를 사용하여 bacterial cellulose 생산에 최적 배지와 배양조건을 설정하였다. Bacterial cellulose를 생성하기 위한 최적배지로는 HS배지보다는 천연사과과즙이 더 우수한 경향을 나타내었으며, 사과과즙에 각종 탄소원을 보강한 결과 탄소원으로 pyruvate가 적합하였다. 탄소원의 농도를 조사한 결과, 1%가 적합하였으며, 각종 질소원의 영향을 조사한 결과 CSL이 가장 우수한 결과를 나타내었다. CSL의 농도에 따른 bacterial cellulose의 생산성을 조사한 결과, 10% 농도에서 가장 좋았다. Bacterial cellulose의 생성에 미치는 배지의 초기 pH의 영향을 조사한 결과 pH 6.0에서 최적이었으며,배양온도별 영향을 조사한 결과 35$^{\circ}C$에서 하는 것이 최적이었다. 최적 배양조건에서 배양시간별로 생성되는bacterial cellulose의 양을 조사한 결과 16일간 배양하는 것이 가장 좋았으며, 이 때 생성되는 bacterial cellulose의 생산량은 8.96 g/L으로 비교적 높았다.

• 한국응용곤충학회지
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• 제49권4호
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• pp.409-416
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• 2010

곤충병원세균인 Photorhabdus temperata ssp. temperata(Ptt)는 곤충의 면역반응을 억제시켜 피기생 곤충 체내에서 공생하는 기주 선충의 발육을 도모하게 된다. 또한 Ptt의 변역억제 활성은 Bacillus thuringiensis(Bt)의 병원성을 증가시킨다. 본 연구는 이러한 유용 곤충병원세균의 대량 생산을 위한 저렴한 배지를 선발하기 위해 수행되었으며, 두 연구용 배지(LB, TSB)와 저렴한 산업용 두 배지(MY, M2)를 상호 비교하였다 모든 배양액에 동일한 밀도의 Ptt를 접종하고 배양하였을 때 48 시간 이후 정지상이 나타났다. 그러나 연구용 배양액인 LB와 TSB에서 두 가지 산업용 배양액보다 정지상에서 높은 세균 밀도를 보였다. 네 가지 배지에서 증식된 Ptt 배양액은 모두 배추좀나방(Plutella xylostella) 3령충에 대한 Bt 병원성을 현격하게 제고시켰고, 이들 배지 종류에 따라 치아가 없었다. 네 가지 배양액에서 세균의 증식에 의해 생산되는 대시물질의 양과 배지별 생산되는 대사물질의 동일성을 확인하기 위해 헥산과 에틸아세테이트의 유기용매로 추출했다. 시간별 배양액의 유기용매 추출물질은 세균의 증식과 비슷하게 대사물질의 생산량에서도 증가하는 것을 알 수 있었다. 역상 HPLC를 이용하여 네 가지 세균 배양액 각각에서 대사물질을 분리하였고, 정량적으로 네 가지 대사물질이 서로 다른 배지에서 통계적으로 차이 없이 검출되었다. 본 연구는 비교적 저렴한 두 가지 산업용 배지가 유용 대사물질의 생성에 변화 없이 Ptt 세균을 저렴하게 배양할 수 있다고 제시하고 있다.

• The Plant Pathology Journal
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• 제15권6호
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• pp.319-322
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• 1999

In order to obtain bacterial metabolites inducing disease resistance in pepper plant, two hundred bacterial isolates were isolated from the rhizosphere soil of tobacco, cucumber, and pepper plant. Ethyl acetate extract of each bacterial culture was used to screening for induction of resistance against phytophthora blight of pepper plant. Application of ethyl acetate extract of an isolate SH122 culture to pepper plant conferred resistance against phytophthora blight consistently and significantly. According to cellular fatty acid analysis and other characteristics, the SH122 culture were significantly lower than those on control plants treated with ethyl acetate extract of nutrient broth. The B. pumilus SH122 itself of ethyl acetate extract of its culture did not show antifungal activity against phytophthora blight in pepper plants.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2003년도 추계학술발표회
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• pp.136-139
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• 2003

The combined effect of bioaugmentation of dechlorinating bacterial cultures and addition of iron powder (Fe$^{0}$ ) on reductive dechlorination of tetrachloroethylene (PCE) and other chlorinated ethylenes in a artificially contaminated soil slurry (60$\mu$mo1es PCE/kg soil) were tested. Two different anaerobic bacterial cultures, a pure bacterial culture of Desulfitobacterium sp. strain Y-51 capable of dechlorinating PCE to cis-1, 2-dechloroethylene (cis-DCE) and the other enrichment culture PE-1 capable of dechlorinating PCE completely to ethylene, were used for the bioaugmentation test. Both treatments introduced with the strain Y-51 and PE-1 culture (3mg dry cell weight/kg soil) showed conversion of PCE to cis-DCE within 40 days. The treatments added with Fe$^{0}$ (0.1 -1.0 %(w/w)) alone to the soil slurry resulted in extended PCE dechlorination to ethylene and ethane and the, dechlorination rate depended on the amount of Fe$^{0}$ added. The combined use of the bacterial cultures with Fe$^{0}$ (0.1-1.0%) showed the higher PCE dechlorination rate than the separated application and the pattern of PCE dechlorination and end-product formation was different from those of the separated application. These results suggested that the combined application of Fe$^{0}$ and the bactrial culture, specially the complete dechlorinating enrichment culture such as PE-1 culture, would be practically effective for remediation of PCE contaminated soil.

• 한국수산과학회지
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• 제48권4호
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• pp.447-453
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• 2015

We determined the optimal culture conditions for obtaining the maximum number of intestinal bacteria from the olive flounder Paralichthys olivaceus, and studied bacterial diversity using both culture-dependent and culture-independent methods. Using six culture conditions, mean bacterial numbers were greater than $10^6$ per gram of gut mucus, regardless of the medium. However, the bacterial diversity, based on colony morphology, appeared much higher on Marine agar (MA) and Zobell 2216 agar than on other media. We found eight and 17 cultured bacterial phylotypes with 99% minimum similarity in gut mucus grown on MA and tryptic soy agar, respectively. Furthermore, we used genomic DNA extracted from gut mucus to generate 78 random clones, which were grouped into 25 phylotypes. Of these, six were affiliated with Firmicutes, Actinobacteria, and Verrucomicrobia, and were not found using our culture-dependent methods. Consequently, we believe that Marine agar and Zobell 2216 agar are optimal media for culturing diverse intestinal microbes; we also discovered several novel sequences not previously recognized as part of the gut microbiota of olive flounder.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제31권1호
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• pp.31-38
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• 2005

Thorough screening of donors medical and social history, extensive serological and bacterial screening combined with developed processing and sterilization methods have improved the safety of the allogeneic tissues in recent decades. The risk of bacterial infection through allogenic tissue transplantation is one of the major problems facing tissue banks. The purpose study is to report the contamination rate in 358 retrieved tissues obtained strictly aseptic conditions, between 2001 and 2002 in Korea Tissue Bank. Samples from 9 donors(total 13 donors) were used in blood culture, and in 7 donors the blood culture were negative. Of the 358 tissues cultured in their entirety, 186(52%) were initially culture negative and 177(48%) were positive. Organism low pathogenicity were cultures from 20.2% of the tissues. To minimize the bacterial load, donors should be obtain in operating rooms, using aseptic techniques with only a few personnel for procurement. The procurement cultures from donors and retrieved tissues with multiple should be carefully interpreted. Blood cultures should be taken account, since these can help to find contamination not detect swab culture. A prospective cohort study is needed to determine which of the varied processing and sterilization methodologies gives the best quality.

• 대한의생명과학회지
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• 제22권4호
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• pp.150-159
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• 2016

Knowledge of the distribution and biodiversity of environmental bacteria and the ecosystem that influences them is crucial for predicting an ecosystem. However, bacterial culture methods can only analyze approximately 0.1% of the existing microorganisms, those that are readily cultured under laboratory conditions. By contrast, next-generation sequencing (NGS) has generally been known to obtain more diverse profiling of bacterial composition. We compared the bacterial communities using both a culture-dependent (MALDI-TOF) and culture-independent (NGS) methods. Environmental specimens were obtained from both freshwater and seawater. Water samples were also analyzed by both pyrosequencing and MiSeq sequencing, in order to select one NGS platform which could analyze comparatively more diverse microbiota. Bacterial distribution analyzed with MALDI-TOF showed no difference between the microbiota of freshwater and seawater, whereas the results analyzed with NGS distinguished between the two. The diversity indexes of MiSeq sequencing were higher than for Pyrosequencing. This indicated that MiSeq sequencing is capable of analyzing a comparatively wider diversity of bacteria. The genus of Flavobacterium and Planktophila were identified as being unique to freshwater, whereas EU801223 and OM43 were found in the seawater. Difference between the bacterial composition of the freshwater and seawater environments was identified by MiSeq sequencing analysis.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권5호
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• pp.289-294
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• 2002

Cellulose production by Acetobacter pasteurianus was investigated in static culture using four bioreactors with silicone rubber membrane submerged in the medium. The shape of the membrane was flat sheet, flat sack, tube and cylindrical balloon. Production rate of cellulose as well as its yield on consumed glucose by the bacteria grown on the flat type membranes was approximately ten-fold greater than those on the non-flat ones in spite of the same membrane thickness. The membrane reactor using flat sacks of silicone rubber membrane as support of bacterial pellicle can supply greater ratio of surface to volume than a conventional liquid surface culture and is promising for industrial production of bacterial cellulose in large scale.