• Parasites, Hosts and Diseases
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• v.37 no.1
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• pp.59-62
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• 1999

Characterization of YS-27, an axenic Entamoeba strain. was performed by three different laboratory methods. Zymodeme analysis using starch gel electrophoresis and PCR with species-specific primers showed that YS-27 is a pathogenic Entamoeba which belongs to the group II zymodeme. Pathogenicity of YS-27 was further confirmed by observing the formation of liver abscess in Mongolian gerbils. These results showed that YS-27 is E. hisolytica.

• Journal of Microbiology and Biotechnology
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• v.29 no.5
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• pp.809-812
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• 2019

Bacterial strains that improve mycelial morphology and growth of white-rot fungi in liquid medium could enhance the impact of white-rot fungi towards lesser recovery of neonicotinoids when cocultured. This was demonstrated by the recovery of clothianidin and acetamiprid from cocultures of the white-rot fungus Phlebia brevispora strains with two mycelial-growth-promoting bacteria, Enterobacter sp. TN3W-14 and Pseudomonas sp. TN3W-8. Clothianidin recovery from cocultures of white-rot fungi and bacteria was over 40% lower than that from axenic microbial cultures and mixed-bacterial cultures. About 20% less acetamiprid was equally recovered from both TMIC33929+TN3W-14 cocultures and mixed-bacterial cultures than from axenic fungal and bacterial cultures.

• Journal of Aquaculture
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• v.11 no.1
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• pp.113-118
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• 1998

This study was carried out to investigate feeding of bacteria by Tigripus japonicus (Copepoda : Harpacticoida) under axenic culture. The ovigerous females and nauplii were grown with feed of aquatic bacteria. Growth of RT bacteria strain was suppressed by feeding of co-existing T. japonicus. T. japonicus of non-axenic culture was observed with oil bead in the egg sac. On the other hand, early nhauplius stage did not develop to the next stage without stage took bacteria as food. And the adult of T. japonicus may utilize the baxteria as nutrient source for egg development.

• Journal of Microbiology
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• v.33 no.3
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• pp.208-214
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• 1995

The effects of various 2, 4-D-degradative plasmids on the axenic growth patterns, the degradation phenotypes, and the competitiveness of different host bacteria were evaluated in liquid cultures; the organisms and plasmids used were Alcaligenes eutrophus JMP134/pJP4, Alcaligenes paradoxus/p2811, Pseudomonas pickettii/p712, pJP4, and p712 or p 2811 exhibited very different restriction fragment profiles in restriction endonuclease digests. These plasmids were transferred to the recipients (P. cepacia and Alcaligenes JMP228) at relatively high frequencies ranging from 8.9 $\times$ 10$^3$ to 1.6 $\times$ 10$^5$ per donar cell. In the axenic liquid cultures the fast-growing strains, such as P. pseudomallei/p745 and P. cepacia/pJP4, exhibited short lag periods, high specific growth rates, and high relative fitness coefficients, while the slow-growing strains, such as P. pickettii/p712 and A. paradoxus/p2811, had long lag periods, low specific growth rates, and low relative fitness coefficients. Depending on the type of plasmid containing the genes for the 2, 4-D pathway, some transconjugants exhibited intermediate grwoth patterns between the fast-growing strains and the slow-growing strains. The plasmid and plasmid-host interactions determined specific growth rate and lag time, respectively, which were shown to be principal determinants of competitiveness among the strains, but relative fitness coefficient derived from the axenic culture was not always predictive for the mixed culture condition.

• Journal of Plant Biotechnology
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• v.48 no.4
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• pp.255-263
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• 2021

Paederia foetida L. is an important medicinal plant that has been used for the treatment of various gastrointestinal related ailments by different tribal communities in India. This plant is also known for its use as a food. Due to overexploitation, P. foetida has been classified as a vulnerable plant in some states of India. The propagation of P. foetida by conventional methods is easy but very slow. Synthetic seed technology offers incredible potential for in vitro propagation of threatened and commercially valuable plants, and can also facilitate the storage and exchange of axenic plant material between laboratories. However, synthetic seed production for P. foetida has not yet been reported. Thus, to the best of our knowledge, the present study is the first attempt to produce synthetic seeds of P. foetida by calcium alginate encapsulation of in vitro regenerated axenic nodal segments. Sodium alginate (3%) and CaCl₂ (100 mM) were found to be the optimal materials for the preparation of ideal synthetic seeds, both in terms of morphology and germination ability. The synthetic seeds showed the best germination (formation of both shoot as well as root; 83.3%) on ½ MS medium augmented with 0.5 mg/L indole-3-acetic acid. The plantlets obtained from these synthetic seeds could be successfully acclimatized under field conditions. We also studied the storage of these synthetic seeds at low temperature and their subsequent sprouting/germination. The seeds showed a germination rate of 63.3% even after 21 days of storage at 4 ℃; thus, they could be useful for transfer and exchange of P. foetida germplasm.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2002.05a
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• pp.313-314
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• 2002

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2001.04a
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• pp.38-41
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• 2001

It has been reported that some plants have the potential to metabolize the 2,4,6-trintroluene (TNT) in contaminated soils, sediments and natural water. In this study, the effects of TNT on germination and early seedling development of Abutilion avicennae was characterized in a germination test. Concentration up to 80 mg/L TNT did not affect germination but root and shoot growth, and fresh biomass decreased as TNT concentration increased. A series of axenic hydroponical batch culture of Abutilion avicennae at various initial TNT concentration was used to determine its transformation kinetics, to identify products formed, and to evaluate phytotoxic effects on the TNT transformation process. At higher initial TNT concentrations, TNT removal rate constant decreased, however, total amount of TNT removed was increased in the culture media. Reductive transformation products of TNT were not detected in the plant culture media but higher concentrations of reduced metabolites were detected in the root and stem extracts of plant material at the 7 days of batch incubation. From these results we concluded that Abutilion avicennae has an intrinsic capacity for taking up and transforming TNT.

• Research in Plant Disease
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• v.9 no.1
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• pp.42-46
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• 2003

Conidiomata of the Rosellinia necatrix were induced in axenic culture under near ultraviolet light radiation. Pieces of sterilized Japanese pear twigs were placed on the 7 days-old oatmeal agar culture in plates. The plates were further incubated far 5 days and then illuminated by under near ultraviolet light radiation. Syn-nemata were developed on twigs in 19 out of 20 isolates tested within 5 weeks, and conidia were observed in 12 out of the 19 isolates. The synnemata and conidia produced were morphologically identical to those of Dematophora necatrix.

• Journal of Life Science
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• v.25 no.5
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• pp.568-576
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• 2015

The marine microalga Pavlova viridis can grow fast and has the ability to accumulate essential nutrients for culturing marine animals, such as EPA and DHA, and it has been used as food for raring larval fish and prawn. The symbiotic relationship between the flagellate microalga Pavlova viridis and its associated bacteria was investigated. An axenic culture of P. viridis was obtained by repeated treatment of the microalga with an antibiotic cocktail. The axenic status was confirmed after sub-culturing three times in a sterile f/2 medium without an antibiotic. The axenic alga was then co-inoculated with five bacteria, arbitrarily designated as I1–I5, isolated from the alga to test the growth promotion of the algae. All bacterial strains promoted the growth of P. viridis, and bacterial isolate I3 was the most effective among the five bacteria tested. The cell number of P. viridis in the co-culture with I3 was significantly higher than that of the control culture. A sequence analysis of the 16S rRNA gene isolated from I3 revealed a 97% nucleotide sequence similarity to that of Citrobacter sp. The growth of strain I3 was also significantly enhanced by co-culturing with P. viridis, indicating a symbiotic relationship between the microalga and its associated bacterium. The association between the microalga and bacterium was confirmed by scanning electron microscopy.

• Korean Aquaculture
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• v.12 no.1
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• pp.95-99
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• 2000

이번 호에서는 먹이생물 관련 양식 정보 시리즈 여섯 번째로 수산 증양식 분야에서 어린 유생의 먹이로서 이용되는 식물${\cdot}$동물 먹이생물의 배양 방법으로서 배량 배양으로 들어가기 전에 선결되어야 할 단일종 배양, 스트레스 배양 그리고 무균 배양에 대하여 기술한다.