Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Regeneration of plants from alginate-encapsulated axenic nodal segments of Paederia foetida L. - A medicinally important and vulnerable plant species

  • Received : 2021.09.22
  • Accepted : 2021.12.21
  • Published : 2021.12.31
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Abstract

Paederia foetida L. is an important medicinal plant that has been used for the treatment of various gastrointestinal related ailments by different tribal communities in India. This plant is also known for its use as a food. Due to overexploitation, P. foetida has been classified as a vulnerable plant in some states of India. The propagation of P. foetida by conventional methods is easy but very slow. Synthetic seed technology offers incredible potential for in vitro propagation of threatened and commercially valuable plants, and can also facilitate the storage and exchange of axenic plant material between laboratories. However, synthetic seed production for P. foetida has not yet been reported. Thus, to the best of our knowledge, the present study is the first attempt to produce synthetic seeds of P. foetida by calcium alginate encapsulation of in vitro regenerated axenic nodal segments. Sodium alginate (3%) and CaCl2 (100 mM) were found to be the optimal materials for the preparation of ideal synthetic seeds, both in terms of morphology and germination ability. The synthetic seeds showed the best germination (formation of both shoot as well as root; 83.3%) on ½ MS medium augmented with 0.5 mg/L indole-3-acetic acid. The plantlets obtained from these synthetic seeds could be successfully acclimatized under field conditions. We also studied the storage of these synthetic seeds at low temperature and their subsequent sprouting/germination. The seeds showed a germination rate of 63.3% even after 21 days of storage at 4 ℃; thus, they could be useful for transfer and exchange of P. foetida germplasm.

Keywords

Acknowledgement

BB gratefully acknowledge the RGNF funding of UGC. The grant of DST, Government of India under DST-FIST programme to the Department of Botany is gratefully acknowledged.

References

  1. Ahmad N, Faisal M, Fatima N, Anis M (2012) Encapsulation of microcuttings for propagation and short-term preservation in Ruta graveolens L.: a plant with high medicinal value. Acta Physiol Plant. 34(6):2303-2310 https://doi.org/10.1007/s11738-012-1031-x
  2. Alatar AA, Ahmad, Javed SB, Abdel-Salam EM, Basahi R, Faisal M (2017) Two-way germination system of encapsulated clonal propagules of Vitex trifolia L.: an important medicinal plant. J Hortic Sci Biotechnol. 92(2):175-182 https://doi.org/10.1080/14620316.2016.1234949
  3. Aquilar NO (2001) Paederia foetida L. In: Van Valkenburg JL, Bunyapraphatsara N (Eds) Plant Resource of South-East Asia. Medicinal and Poisonous Plants. Ledien, The Netherlands, Blackhuys Publisher, 2001, pp 396-400
  4. Ara H, Jaiswal U, Jaiswal VS (2000) Synthetic seed: prospects and limitations. Curr Sci. 78(12):1438-1444
  5. Begum J, Yusuf M, Chowdhury JU, Khan S, Anwar MN (2007) Antifungal activity of forty higher plants against phytopathogenic fungi. Bangladesh J Microbiol. 24:76-78
  6. Behera B, Behera S, Jena PK, Barik DP, Naik SK (2017) Adventitious shoot organogenesis and plant regeneration from internode explants of Paederia Foetida L.: a valuable medicinal plant. Biosci Biotech Res Asia. 14(3):893-900 https://doi.org/10.13005/bbra/2523
  7. Behera B, Sinha P, Gouda S, Rath SK, Barik DP, Jena PK, Panda PC, Naik SK (2018) In vitro propagation by axillary shoot proliferation, assessment of antioxidant activity, and genetic fidelity of micropropagated Paederia foetida L. J App Biol Biotech. 6(2):41-49
  8. Behera S, Rout, KK, Panda, PC, Naik SK (2020). Production of non-embryogenic synthetic seeds for propagation and germplasm transfer of Hedychium coronarium J. Koenig. J Appl Res Med Arom Plant, 19, 100271
  9. Bose PK, Banerjee AK, Ghosh C (1953) Chemical investigation of Paederia foetida Linn. Transaction of Bose Research Institute. 19:77-78
  10. Bukhari N, Siddique I, Perveen K, Siddiqui I, Alwahibi M (2014) Synthetic seed production and physio-biochemical studies in Cassia angustifolia Vahl. - a medicinal plant. Acta Biol Hung. 65(3):355-367 https://doi.org/10.1556/ABiol.65.2014.3.11
  11. Chanda S, Sarethy IP, De B, Singh K (2013) Paederia foetida - a promising ethno-medicinal tribal plant of north-eastern India. J Forestry Res. 24:801-808 https://doi.org/10.1007/s11676-013-0369-2
  12. Chopra RN, Chopra IC, Verma BS (1969) Supplement to glossary of Indian medicinal plants. New Delhi: CSIR
  13. Danso KE, Ford-Lloyd BV (2003) Encapsulation of nodal cuttings and shoot tips for storage and exchange of Cassava germplasm. Plant Cell Rep. 21(8):718-725 https://doi.org/10.1007/s00299-003-0594-9
  14. Fatima N, Ahmad N, Anis M, Ahmad I (2013) An improved in vitro encapsulation protocol, biochemical analysis and genetic integrity using DNA based molecular markers in regenerated plants of Withania somnifera L. Ind Crops Prod. 50:468-477 https://doi.org/10.1016/j.indcrop.2013.08.011
  15. Fujii JA, Slade D, Redenbaugh K, Walker KA (1987) Artificial seeds for plant propagation. Trends Biotechnol. 5:335-339 https://doi.org/10.1016/0167-7799(87)90018-7
  16. Gantait S, Kundu S, Ali N, Sahu NC (2015) Synthetic seed production of medicinal plants: a review on influence of explants, encapsulation agent and matrix. Acta Physiol Plant. 37:98 https://doi.org/10.1007/s11738-015-1847-2
  17. Gantait S, Kundu S, Yeasmin L, Ali MN (2017a) Impact of differential levels of sodium alginate, calcium chloride and basal media on germination frequency of genetically true artificial seeds of Rauvolfia serpentina (L.) Benth. ex Kurz. J Appl Res Med Aromat Plant. 4:75-81 https://doi.org/10.1016/j.jarmap.2017.01.005
  18. Gantait S, Vijayan J, Majee A (2017b) Artificial seed production of Tylophora indica for interim storing and swapping of germplasm. Horticult Plant J. 3(1): 41-46 https://doi.org/10.1016/j.hpj.2017.06.004
  19. Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research. John Wiley & Sons
  20. Grzegorczyk I, Wysokinska H (2011) A protocol for synthetic seeds from Salvia officinalis L. shoot tips. Acta Biol Crac Ser Bot. 53(1):80-85
  21. Haque SM, Ghosh B (2014) Somatic embryogenesis and synthetic seed production-a biotechnological approach for true-to-type propagation and in vitro conservation of an ornamental bulbaceous plant Drimiopsis kirkii Baker. Appl Biochem Biotechnol. 172(8):4013-4024 https://doi.org/10.1007/s12010-014-0817-2
  22. Hegazi GA (2011) Viability of encapsulated shoot tips of Capparis orientalis Duh. J Nat Sci. 9(8):223-228
  23. Jahan AA, Anis M (2015) Retrieval of plantlets from cryopreserved alginate buds of Nyctanthes arbortristis L. - an effectual approach for germplasm conservation. Int J Dev Res. 5(5):4397-4402
  24. Jain P, Danwra K, Sharma HP, Mahato D (2018) In vitro tissue culture studies and synthetic seed formation from Plumbago zeylanica L. Indian J Exp Biol. 56:769-773
  25. Khan MK, Sharma T, Misra P, Shukla PK, Singh Y, Ramteke PW (2013) Production of plantlets on different substrate from encapsulated in vitro nodal explants of Stevia rebaudiana. Int J Recent Sci Res. 4(3):211-215
  26. Kumar GK, Thomas TD (2012) High frequency somatic embryogenesis and synthetic seed production in Clitoria ternatea Linn. Plant Cell Tiss Org Cult. 110(1):141-151 https://doi.org/10.1007/s11240-012-0138-5
  27. Kumar V, Anwar F, Ahmed D, Verma A, Ahmed A, Damanhouri ZA, Mishra V, Ramteke PW, Bhatt PC, Mujeeb M (2014) Paederia foetida Linn. Leaf extract: an antihyperlipidemic, antihyperglycaemic and antioxidant activity. BMC Complement Altern Med. 14:76 https://doi.org/10.1186/1472-6882-14-76
  28. Mehrotra S, Khwaja O, Kukreja AK, Rahman L (2012) ISSR and RAPD based evaluation of genetic stability of encapsulated micro shoots of Glycyrrhiza glabra following 6 months of storage. Mol Biotechnol. 52(3):262-268 https://doi.org/10.1007/s12033-011-9491-6
  29. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tabacco tissue cultures. Physiol Plant. 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  30. Naik SK, Chand PK (2006) Nutrient-alginate encapsulation of in vitro nodal segments of pomegranate (Punica granatum L.) for germplasm distribution and exchange. Sci Hortic. 108:247-252 https://doi.org/10.1016/j.scienta.2006.01.030
  31. Osman H, Rahim AA, Isa NM, Bakhir NM (2009) Antioxidant activity and phenolic content of Paederia foetida and Syzygium aqueum. Molecules. 14:970-978 https://doi.org/10.3390/molecules14030970
  32. Pattnaik S, Chand PK (2000) Morphogenic response of the alginate-encapsulated axillary buds from in vitro shoot cultures of six mulberries. Plant Cell Tiss Org Cult. 60(3):177-185 https://doi.org/10.1023/A:1006424626433
  33. Perveen S, Anis M (2014) Encapsulation of internode regenerated adventitious shoot buds of Indian Siris in alginate beads for temporary storage and two-fold clonal plant production. Acta Physiol Plant. 36(8):2067-2077 https://doi.org/10.1007/s11738-014-1584-y
  34. Posposilova J, Ticha I, Kadlecek P, Haisel D, Plzakova S (1999) Acclimatization of micropropagated plants to ex vitro conditions. Biol Plant. 42(4):481-497 https://doi.org/10.1023/A:1002688208758
  35. Qahtan AA, Abdel-Salam EM, Alatar AA, Wang QC, Faisal M (2019) An introduction to synthetic seeds: production, techniques, and applications. In: Faisal M, Alatar A (Eds) Synthetic Seeds. Springer, Cham, pp 1-20
  36. Rai MK, Asthana P, Singh SK, Jaiswal VS, Jaiswal U (2009) The encapsulation technology in fruit plants-a review. Biotechnol Adv. 27(6):671-679 https://doi.org/10.1016/j.biotechadv.2009.04.025
  37. Rathore MS, Kheni J (2017) Alginate encapsulation and in vitro plantlet regeneration in critically endangered medicinal plant, Withania coagulans (Stocks) Dunal. Proc Nat Acad Sci, India, Sec Biol Sci. 87(1):129-134 https://doi.org/10.1007/s40011-015-0577-y
  38. Redenbaugh K, Paasch BD, Nichol JW, Kossler ME, Viss PR, Walker KA (1986) Somatic seeds: encapsulation of asexual plant embryos. Nat Biotechnol. 4(9):797-801 https://doi.org/10.1038/nbt0986-797
  39. Redenbaugh K, Slade D, Viss PR, Fujii J (1987) Encapsulation of somatic embryos in synthetic seed coats. Hort Sci. 22:803-809
  40. Rency AS, Satish L, Pandian S, Rathinapriya P, Ramesh M (2017) In vitro propagation and genetic fidelity analysis of alginate-encapsulated Bacopa monnieri shoot tips using Gracilaria salicornia extracts. J Appl Phycol. 29(1):481-494 https://doi.org/10.1007/s10811-016-0918-0
  41. Sharma S, Shahzad A, Mahmood S, Saeed T (2015) High-frequency clonal propagation, encapsulation of nodal segments for short-term storage and germplasm exchange of Ficus carica L. Trees. 29(2):345-353 https://doi.org/10.1007/s00468-014-1114-y
  42. Singh SK, Rai MK, Asthana P, Sahoo L (2010) Alginate-encapsulation of nodal segments for propagation, short-term conservation and germplasm exchange and distribution of Eclipta alba (L.). Acta Physiol Plant. 32:607-610 https://doi.org/10.1007/s11738-009-0444-7
  43. Srivastava SK, Srivastava N (2004) In vitro multiplication of Paedaria foetida L.- a rare medicinal plant. J Plant Biochem Biotechnol. 13:89-91 https://doi.org/10.1007/BF03263200
  44. Srivastava V, Khan SA, Banerjee S (2009) An evaluation of genetic fidelity of encapsulated microshoots of the medicinal plant: Cineraria maritima following six months of storage. Plant Cell Tiss Org Cult. 99(2):193-198 https://doi.org/10.1007/s11240-009-9593-z
  45. Standardi A, Piccioni E (1998) Recent perspectives on synthetic seed technology using nonembryogenic in vitro - derived explants. Int J Plant Sci. 159(6):968-978 https://doi.org/10.1086/314087
  46. Sundararaj SG, Agrawal A, Tyagi RK (2010) Encapsulation for in vitro short-term storage and exchange of ginger (Zingiber officinale Rosc.) germplasm. Sci Hort. 125(4):761-766 https://doi.org/10.1016/j.scienta.2010.06.001
  47. Ved DK, Kinhal GA, Ravi Kumar K, Vijaya Shankar R, Sumathi R, Mahapatra AK and Panda PC (Eds.) (2008) Conservation assessment and management prioritization for medicinal plants of Orissa. Regional Plant Research Centre and Foundation for Revitalization of Local Health Traditions, Bhubaneswar and Bangalore
  48. Verma SK, Choudhary DK, Kumar AA, Lal M (2015) Plant regeneration of A. lakoocha from encapsulated nodal explants. Arch Appl Sci Res. 7(1):22-27
  49. Verma SK, Rai MK, Asthana P, Jaiswal VS, Jaiswal U (2010) In vitro plantlets from alginate-encapsulated shoot tips of Solanum nigrum L. Sci Hort. 124(4):517-521 https://doi.org/10.1016/j.scienta.2010.02.002