• BMB Reports
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• v.54 no.10
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• pp.505-515
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• 2021

Human pluripotent stem cells (hPSCs) include human embryonic stem cells (hESCs) derived from blastocysts and human induced pluripotent stem cells (hiPSCs) generated from somatic cell reprogramming. Due to their self-renewal ability and pluripotent differentiation potential, hPSCs serve as an excellent experimental platform for human development, disease modeling, drug screening, and cell therapy. Traditionally, hPSCs were considered to form a homogenous population. However, recent advances in single cell technologies revealed a high degree of variability between individual cells within a hPSC population. Different types of heterogeneity can arise by genetic and epigenetic abnormalities associated with long-term in vitro culture and somatic cell reprogramming. These variations initially appear in a rare population of cells. However, some cancer-related variations can confer growth advantages to the affected cells and alter cellular phenotypes, which raises significant concerns in hPSC applications. In contrast, other types of heterogeneity are related to intrinsic features of hPSCs such as asynchronous cell cycle and spatial asymmetry in cell adhesion. A growing body of evidence suggests that hPSCs exploit the intrinsic heterogeneity to produce multiple lineages during differentiation. This idea offers a new concept of pluripotency with single cell heterogeneity as an integral element. Collectively, single cell heterogeneity is Janus-faced in hPSC function and application. Harmful heterogeneity has to be minimized by improving culture conditions and screening methods. However, other heterogeneity that is integral for pluripotency can be utilized to control hPSC proliferation and differentiation.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.19 no.9
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• pp.1719-1729
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• 1994

In this paper, an adaptive CAC(Connection Admission Control) method is proposed. The adaptive CAC uses traffic estimates derived from both traffic parameter specified by user and cell flow measurements. Traffic estimation using user-specified parameters is performed at every moment of connection request or connection release by recursive formula which makes real-time calculation possible. Traffic estimation using cell flow measurement is carried out when the number of connected calls does not change during a measurement reflection period-renewal period. The most import ant thing for the traffic estimation using cell flow measurement is the determination of the length of a renewal period to trace a real traffic flow with an allowable time lag and the measurement reflection ratio(MRR) both to reduce the portion of overestimation and to avoid underestimation of real traffic flow. To solve these problems, the adaptive CAC updates renewal period and MRR adaptively according to the number of connections and the elapsed time after last connection or release respectively. Performance analysis for the proposed method is evaluated in several aspects for the cases of both homogeneous and heterogeneous bursty traffic. Numerical examples show the adaptive CAC method has the better performance compared with conventional CAC method based on burst model from the both utilization and QOS point of view.

• Journal of KIISE:Information Networking
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• v.28 no.4
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• pp.527-539
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• 2001

The wireless asynchronous transfer mode (ATM) system has the advantage of providing the broadband services with various quality-of-service requirements to the mobile terminal efficiently by utilizing the ATM technology developed for the wired ATM system. The available bit rate (ABR) service among various ATM services utilizes the available bandwidth remaining in the ATM link, which allows the efficient bandwidth usage. During the handoff of the mobile terminal, however, the queue length in the access point (AP) which resides in the boundary of the wired ATM network and the wireless ATM network may increase abruptly. In this paper, we propose a scheme which prevents the buffer-overflow and buffer-underflow in the AP during the handoff of the wireless ABR connection in the wireless ATM system using binary feedback rate-based ABR traffic control. This scheme controls the source's cell generation rate during both handoff period and some time interval after the completion of the handoff procedure. The simulation results show that the proposed scheme prevents the buffer-overflow and buffer-underflow. The proposed scheme can contribute to increasing the throughput of the wireless ABR service during handoff by preventing the buffer overflow and underflow during handoff period.

• The Journal of Natural Sciences
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• v.9 no.1
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• pp.1-7
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• 1997

Calcium/calmodulin-dependent protein kinase II (CaMK-II) is responsible for the phosphorylation of proteins involved in various cellular functions. Since the level of intracellular calcium ($Ca_2+$) oscillate during the cell cycle, it is expected that the activity of CaMK-II is also dependent on the cell cycle. The kinase activity in NIH3T3 cells which were arrested at or released from certain phase of the cell cycle was measured and compared to that in the normally growing asynchronous control cells to investigate whether the activity of this kinase is cell cycle-dependent. Cells were arrested at G0, G1, G1/S, G2/M and M phase, respectively by use of various drugs which do not have any effect on the kinase activity of CaMK-II at G0, G1, G1/s and G2/M phase was similar to that of the control cells, whereas lower at M. Calcium-independent activity of CaMK_II by autophosphorylation was higher at M and, thus, higher autonomy at M, which represented the physiologically relevant activity of CaMK-II. A similar pattern of activity change of the kinase was demonstrated during the cell cycle of synchronized cells which were released from G1 arrest. These results indicate that the activity of CaMK-11 is cell cycle-dependent and is activity during the mitosis.

• Journal of Advanced Marine Engineering and Technology
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• v.37 no.4
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• pp.409-417
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• 2013

Mu-communication board supported by multi-communication is designed with Atxmega 128A1 which is a low power energy consuming of 8-bit microcontroller. ATxmega128A1 microcontroller consists of 8 UART(Universal asynchronous receiver/transmitter) ports which can be setting appropriate user interface having command line interpreter(CLI) program with each port, 2 kbytes EEPROM, 128 kbytes flash memory, 8 kbytes SRAM. 8 URAT ports are used for the multi communication modem, GPS module, etc. and EEPROM is used for saving a configuration for program running, and flash memory of 128 kbytes is used for storing a Firm Ware, and 8 kbytes SRAM is used for stack, storing memory of global variables while program running. If we uses the hybrid communication of path optimization of VHF, TRS and CDMA to remote control AtoN(aid to navigation), it is able to remove the communication shadow area. Even though there is a shadow area for individual communication method, we can select an optimum communication method. The compatibility of data has been enhanced as using of same data frame per communication devices. For the test, 8640 of data has been collected from the each buoy during 30 days in every 5 minutes and the receiving rate of the data has shown more than 99.4 %.

• Journal of KIISE:Computing Practices and Letters
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• v.6 no.2
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• pp.120-134
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• 2000

In this paper we present a Distributed Object oriented Virtual computing Environment, called DOVE which consists of autonomous distributed objects interacting with one another via method invocations based on a distributed object model. DOVE appears to a user logically as a single virtual computer for a set of heterogeneous hosts connected by a network as if objects in remote site reside in one virtual computer. By supporting efficient parallelism, heterogeneity, group communication, single global name service and fault-tolerance, it provides a transparent and easy-to-use programming environment for parallel applications. Efficient parallelism is supported by diverse remote method invocation, multiple method invocation for object group, multi-threaded architecture and synchronization schemes. Heterogeneity is achieved by automatic data arshalling and unmarshalling, and an easy-to-use and transparent programming environment is provided by stub and skeleton objects generated by DOVE IDL compiler, object life control and naming service of object manager. Autonomy of distributed objects, multi-layered architecture and decentralized approaches in hierarchical naming service and object management make DOVE more extensible and scalable. Also,fault tolerance is provided by fault detection in object using a timeout mechanism, and fault notification using asynchronous exception handling methods

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.6
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• pp.1107-1114
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• 2004

System Packet Interface Level 4 Phase 2(SPI-4.2) is an interface for packet and cell transfer between a physical layer(PHY) device and a link layer device, for aggregate bandwidths of OC-192 ATM and Packet Over Sonet/SDH(POS), as well as 10Gbps Ethernet applications. SPI-4.2 core consists of Tx and Rx modules and supports full duplex communication. Tx module of SPI-4.2 core writes 64-bit data word and 14-bit header information from the user interface into asynchronous FIFO and transmits DDR(Double Data Rate) data over PL4 interface. Rx module of SPI-4.2 core operates in vice versa. Tx and Rx modules of SPI-4.2 core are designed to support maximum 256-channel and control the bandwidth allocation by configuring the calendar memory. Automatic DIP4 and DIP-2 parity generation and checking are implemented within the designed core. The designed core uses Xilinx ISE 5.li tool and is described in VHDL Language and is simulated by Model_SIM 5.6a. The designed core operates at 720Mbps data rate per line, which provides an aggregate bandwidth of 11.52Gbps. SPI-4.2 interface core is suited for line cards in gigabit/terabit routers, and optical cross-connect switches, and SONET/SDH-based transmission systems.

• Journal of Industrial Convergence
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• v.21 no.8
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• pp.1-12
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• 2023

The purpose of the study is to analyze the impact of blended learning's external classroom formats and internal teaching strategies, which has been implemented in university classes due to COVID-19, on students' academic achievement and learners' perceptions, as well as to provide insights into the desirable direction of online education. The study was conducted during the 1st semester of 2022 at G University, targeting students taking Calculus I. The experimental group consisted of 117 students, while the control group consisted of 707 students. Blended learning, involving a combination of face-to-face classes, online classes, and mixed teaching methods, was implemented, and academic achievement and learner perceptions were assessed. The research findings indicate that compared to solely online classes, adopting a blended learning approach with online classes before the midterm and face-to-face classes afterwards resulted in a decline in academic achievement. The unprepared and simplistic external format of blended learning was found to be ineffective, however, a blended learning model consisting solely of online classes, incorporating a mix of asynchronous and synchronous instruction, demonstrated positive learner perceptions. Additionally, utilizing technology in the teaching strategies yielded positive outcome.