• Journal of Microbiology and Biotechnology
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• 제14권3호
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• pp.593-598
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• 2004

The arsenical resistance (ars) operon was cloned from a 67-kilobase pair (kb) plasmid, which was previously shown to be responsible for arsenic salts resistance in K. oxytoca D12. When plasmid pAE48, carrying the ars operon, was transformed into E. coli, transformed cells displayed enhanced survival in the presence of 4 mM arsenite, 50 mM arsenate, or 0.4 mM antimonite. The nucleotide sequence of the 5.6-kb fragment encoding arsenical resistance revealed five open reading frames (ORFs), which were predicted to encode polypeptides of 12.8 (arsR), 13.4 (arsD), 62.6 (arsA), 45 (arsB), and 16.7 (arse) kilodaltons (kDa). Each ORF was preceded by a ribosome binding site. A putative promoter-like sequence was identified upstream of arsR, and a possible termination site was found downstream of arsC. When the deduced amino acid sequences of the K. oxytoca Dl2 Ars proteins were compared with the amino acid sequences of the E. coli R773 Ars proteins, a significant amino acid similarity was observed (87.9％ for ArsR, 89.2％ for ArsD, 83.2％ for ArsA, 92.6％ for ArsB, and 91.3％ for ArsC), suggesting an evolutionary relationship of the ars genes of E. coli plasmid R773 and K. oxytoca Dl2.

• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.812-821
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• 2007

The ecosystems of certain abandoned mines contain arsenic-resistant bacteria capable of performing detoxification when an ars gene is present in the bacterial genome. The ars gene has already been isolated from Pseudomonas putida and identified as a member of the membrane transport regulatory deoxyribonucleic acid family. The arsenite-oxidizing bacterial strains isolated in the present study were found to grow in the presence of 66.7 mM sodium arsenate($V;\;Na_2HAsO_4{\cdot}7H_2O$), yet experienced inhibited growth when the sodium arsenite($III;\;NaAsO_2$) concentration was higher than 26 mM. Batch experiment results showed that Pseudomonas putida strain OS-5 completely oxidized 1 mM of As(III) to As(V) within 35 h. An arsB gene encoding a membrane transport regulatory protein was observed in arsenite-oxidizing Pseudomonas putida strain OS-5, whereas arsB, arsH, and arrA were detected in strain OS-19, arsD and arsB were isolated from strain RW-18, and arsR, arsD, and arsB were found in E. coli strain OS-80. The leader gene of arsR, -arsD, was observed in a weak acid position. Thus, for bacteria exposed to weak acidity, the ars system may cause changes to the ecosystems of As-contaminated mines. Accordingly, the present results suggest that arsR, arsD, arsAB, arsA, arsB, arsC, arsH, arrA, arrB, aoxA, aoxB, aoxC, aoxD, aroA, and aroB may be useful for arsenite-oxidizing bacteria in abandoned arsenic-contaminated mines.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2006년도 한국약용작물학회 공동춘계학술발표회
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• pp.292-293
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• 2006

• 한국IT서비스학회:학술대회논문집
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• 한국IT서비스학회 2009년도 추계학술대회
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• pp.409-412
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• 2009

고객과 접하는 통화수단으로 VRS의 필요성이 대두되고 있다. 기존 ARS를 통해 서비스를 하고 있는 사업체의 경우 VRS는 또하나의 새로운 시스템 도입이라는 점과 기존 고객에게 접근하는데 있어서 큰 변화를 느끼지 않으면서 보다 쉽고, 편리하게 다가 갈 수 있어야 한다는 점에서 고민을 안겨준다. 본 논문에서는 기존 ARS 시스템을 이용하여 쉽게 VRS를 적용할 수 있는 방법을 제시하고, 향후 VRS의 발전방향을 제시한다.

• 음성과학
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• 제8권2호
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• pp.61-71
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• 2001

Speech material, which is collected from ARS(Automatic Response System), was analyzed and classified into disease and non-disease state. The material include 11 different kinds of diseases. Along with ARS speech, DAT(Digital Audio Tape) speech is collected in parallel to give the bench mark. To analyze speech material, analysis tools, which is developed local laboratory, are used to provide an improved and robust performance to the obtained parameters. To classify speech into disease and non-disease class, multi-layered neural network was used. Three different combinations of 3, 6, 12 parameters are tested to obtain the proper network size and to find the best performance. From the experiment, the classification rate of 92.5% was obtained.

• 기술혁신연구
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• 제20권3호
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• pp.181-198
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• 2012

경기도는 도(道)의 지원으로 성공한 기업의 초과 이윤을 지역으로 환원시킬 수 있는 제도의 틀을 마련하기 위하여 2011년도에 성공기술료 제도를 도입하였다. 향후 성공기술료 징수를 통해 가용 재원 감소기에 지자체의 과학기술 정책 추진을 위한 추가적인 재원을 확보하고, 기술개발사업의 성과를 설명할 수 있는 근거를 확보할 수 있을 것으로 기대한다. 그러나 부담능력이 없는 기업에 추가적인 기술료를 징수함으로써 기업의 혁신에 대한 인센티브를 저하할 가능성에 대한 우려가 있다. 본 연구는 사례분석을 통해서 성공기술료 발생 시의 기업의 부담능력을 분석하는 것으로 목적으로 한다. 분석결과로부터 성공기술료는 실질적으로 부담능력이 있는 기업에만 징수하게 됨으로써 제도의 도입이후에도 기업의 혁신 성공 인센티브를 유지하여 공공기술개발 사업의 정책적 목표를 달성할 수 있는 것으로 나타났다. 그러나 징수의 객관성 확립, 기술사업화의 체계적 지원확대, 고정기술료율 인하로 적정한 기대기술료 수준 유지 등의 제도적 개선이 필요한 것으로 나타났다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2006년도 한국약용작물학회 공동춘계학술발표회
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• pp.232-233
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• 2006

• Journal of Microbiology and Biotechnology
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• 제3권1호
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• pp.6-11
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• 1993

An autonomously replicating sequence (Kf-ARS1) of Kluyveromyces fragilis was cloned from the genomic library which was constructed using pHN134 as a cloning vector to make a new host-vector system for the production of heterologous protein from K. fragilis as a host. The cloning vector pHN134 was composed of $Km^r, Ap^r$ and multiple cloning site in LacZ . A clone carrying Kf-ARS1 was isolated and the recombinant plasmid was designated as pIKD102. The cloned fragment was 2.3 kb (EcoRI/EcoRI) in length. Subcloning experiment showed that the region for ARS activity was 1.5 kb (SalI/EcoRI) fragment. It was shown that the Kf-ARS1 was active in Saccharomyces cerevisiae and Kluyveromyces fragilis.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2001년도 춘계심포지움 및 학술발표회
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• pp.141-141
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• 2001

The arsenic resistance operon from pMH12 in Klebsiella oxytoca contains two regulatory genes. The first open reading frame for arsR extend up to 348 bp and has a translational product corresponding to a protein of 116 amino acid residue polypeptide with a molecular mass of 13 kDa. And the second ORF for arsD extend up to 360 bp and express a protein of 120 amino anid residue polypeptide with 13kDa. (omitted)

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.282.1-282
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• 1999

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