Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning and Characterization of Autonomously Replicating Sequence(ARS) from Kluyveromyces fragilis

  • HONG, SOON-DUCK (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • JONG-GUK KIM (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • TAKUYA NAGAMATSU (Center of Research and Development, Ikeda Mohando Co., LTD) ;
  • JOO-HYUN NAM (Taegu Technical Junior College) ;
  • DONG-SUN LEE (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • SANG-YONG LEE (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • SUN-HWA HA (Department of Microbiology, College of Natural Sciences, Kyungpook National University)
  • Published : 1993.03.01
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Abstract

An autonomously replicating sequence (Kf-ARS1) of Kluyveromyces fragilis was cloned from the genomic library which was constructed using pHN134 as a cloning vector to make a new host-vector system for the production of heterologous protein from K. fragilis as a host. The cloning vector pHN134 was composed of $Km^r, Ap^r$ and multiple cloning site in LacZ . A clone carrying Kf-ARS1 was isolated and the recombinant plasmid was designated as pIKD102. The cloned fragment was 2.3 kb (EcoRI/EcoRI) in length. Subcloning experiment showed that the region for ARS activity was 1.5 kb (SalI/EcoRI) fragment. It was shown that the Kf-ARS1 was active in Saccharomyces cerevisiae and Kluyveromyces fragilis.

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