Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Characterization of the Plasmid-Encoded Arsenic Salts Resistance Determinant from Klebsiella oxytoca D12

  • Rhie, Ho-Gun (Department of Biology, Research Institute for Basic Science, Kyung Hee University) ;
  • Lee, Sung-Jae (Department of Biology, Research Institute for Basic Science, Kyung Hee University) ;
  • Lee, Ho-Sa (Department of Biology, Research Institute for Basic Science, Kyung Hee University)
  • Published : 2004.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The arsenical resistance (ars) operon was cloned from a 67-kilobase pair (kb) plasmid, which was previously shown to be responsible for arsenic salts resistance in K. oxytoca D12. When plasmid pAE48, carrying the ars operon, was transformed into E. coli, transformed cells displayed enhanced survival in the presence of 4 mM arsenite, 50 mM arsenate, or 0.4 mM antimonite. The nucleotide sequence of the 5.6-kb fragment encoding arsenical resistance revealed five open reading frames (ORFs), which were predicted to encode polypeptides of 12.8 (arsR), 13.4 (arsD), 62.6 (arsA), 45 (arsB), and 16.7 (arse) kilodaltons (kDa). Each ORF was preceded by a ribosome binding site. A putative promoter-like sequence was identified upstream of arsR, and a possible termination site was found downstream of arsC. When the deduced amino acid sequences of the K. oxytoca Dl2 Ars proteins were compared with the amino acid sequences of the E. coli R773 Ars proteins, a significant amino acid similarity was observed (87.9％ for ArsR, 89.2％ for ArsD, 83.2％ for ArsA, 92.6％ for ArsB, and 91.3％ for ArsC), suggesting an evolutionary relationship of the ars genes of E. coli plasmid R773 and K. oxytoca Dl2.

Keywords

References

  1. J. Bacteriol. v.175 Arsenic efflux governed by the arsenic resistance determinant of Staphylococcus aureus plasmid pl258 Broser,S.;J.I.Guangyong;A.Broer;S.Silver https://doi.org/10.1128/jb.175.11.3480-3485.1993
  2. FEMS Microbiol. Lett. v.139 The arsenical resistance operon if IncN plasmid R46 Bruhn,D.F.;J.Li;S.Sliver;F.Roberto;B.P.Rosen https://doi.org/10.1111/j.1574-6968.1996.tb08195.x
  3. J. Bacteriol. v.161 Separate resistances to arsenate and arsenite(antimonate)encoded by the arsenical resistance operon of R factor R773 Chen,C.;H.L.Mobley;B.P.Rosen
  4. J. Biol. Chem. v.261 Nucleotide sequence of the structural genes for an anion pump Chen,C.M.;T.K.Misra;S.Sliver;B.P.Rosen
  5. Kor. J. Microbiol. v.29 Genetic characteristics of arsenic compounds-resistant bacteria isolated from stream water Chung,M.K.;H.S.Lee
  6. Kor. J. Microbiol. v.31 Construction of a cloning vector carrying arsenic compound resistant genes Chung,M.K.;N.J.Chung;H.S.Lee
  7. EMBO J. v.10 The MinD protein is a membrane ATPase required for the correct placement of the Escherichia coli division site de Boer;P.A.J.;R.E.Crossley;A.R.Hand;L.I.Rothfield
  8. J. Bacteriol. v.177 Dual mode of energy coupling by the oxyanion-translocating ArsB protein Dey,S.;B.P.Rosen https://doi.org/10.1002/path.1711770409
  9. J. Biol. Chem. v.266 Substrate-induced dimerization of the ArsA protein,the catalytic component of an anion-translocating ATPase Hsu,C.M.;P.Kaur;C.E.Karkaria;R.F.Steiner;B.P.Rosen
  10. Proc. Natl. Acad. Sci. USA v.89 Reduction of arsenate to arsenite by the ArsC protein of the arseinc resistance operon of Staphylococcus aureus plasmid pI258 Ji,G.;S.Sliver https://doi.org/10.1073/pnas.89.20.9474
  11. J. Bacteriol. v.174 Regulation and expression of the arsenic resistance operon from Staphylococcus aureus plasmid pI258 Ji,G.;S.Silver https://doi.org/10.1128/jb.174.11.3684-3694.1992
  12. Biochemistry v.33 Arsenate reductase of Staphylococcus aureus plasmid pI258 Ji,G.;E.A.Garber;L.G.Armes;C.M.Chen;J.A.Fuchs;S.Silver https://doi.org/10.1021/bi00189a034
  13. Biochemistry v.33 Identification of the site of [α-$^{32}P$]ATP adduct formation in the ArsA protein Kaur,P.;B.P.Rosen https://doi.org/10.1021/bi00187a010
  14. J. Microbiol. Biotechnol. v.11 Effect of ArsA,arsenite-specific ATPase,on inhibition of cell division in Escherichia coli Lee,S.J.;S.C.Lee;S.H.Choi;M.K.Chung;H.G.Rhie;H.S.Lee
  15. Mol. Gen. Genet. v.191 Cloning and expression of R-factor mediated arsenate resistance in Escherichia coli Mobley,H.L.;T.C.Chen;S.Sliver;B.P.Rosen https://doi.org/10.1007/BF00425757
  16. J. Bacteriol. v.181 MinDE-dependent pole-to-pole oscillation of division inhibitor MinC in Escherichia coli Raskin,D.M.;P.A.J.de Boer
  17. Biochem. Biophys. v.284 Molecular analysis of an anion pump:purification of the ArsC protein Rosen,B.P.;U.Weigel;R.A.Monticello;B.P.F.Edwards https://doi.org/10.1016/0003-9861(91)90312-7
  18. Molecular Cloning:A Laboratory Manual,2nd Ed. Sambrook,J.;E.F.Fritsch;T.Maniatis
  19. Mol. Microbiol. v.3 Identification of the membrane component of the anion pump encoded by the arsenical resistance operon of plasmid R773 M.J.D.;L.S.Tisa;B.P.Rosen https://doi.org/10.1111/j.1365-2958.1989.tb00098.x
  20. Nucleic Acids Res, v.18 Identification of the metalloregulatory element of the plasmid-encoded arsenical resistance operon M.J.D.;C.L.Hope;J.B.Owolabi;L.S.Tisa;B.P.Rosen https://doi.org/10.1093/nar/18.3.619
  21. J. Biol. Chem. v.269 Identification of putative metal binding site in a new family of metalloregulatory proteins Shi,W.;J.Wu;B.P.Rosen
  22. J. Biol. Chem. v.271 The role of arsenic-thiol interactions in metalloregulation of the ars operon Shi,W.;J.Dong;R.A.Scott;M.Y.Kzenzenko;B.P.Rosen https://doi.org/10.1074/jbc.271.16.9291
  23. Annu. Rev. Microbiol. v.42 Plasmid-mediated heavy metal resistance Silver,S.;T.K.Misra https://doi.org/10.1146/annurev.mi.42.100188.003441
  24. Proc, Natl. Acad. Sci. USA v.82 A bacteriophage T7 RNA polymerase/promoter system for controlled expression of specific genes Tabor,S.;C.C.Richardson https://doi.org/10.1073/pnas.82.4.1074
  25. J. Bioeng. Biomembr. v.22 Transprot system encoded by bacterial plasmids Tisa,L.S.;B.P.Rosen https://doi.org/10.1007/BF00762959
  26. J. Biol. Chem. v.265 Molecular characterization of an anion pump Tisa,L.S.;B.P.Rosen
  27. Mol. Microbiol. v.5 The ArsR protein is a transacting regulatory protein Wu,J.;B.P.Rosen https://doi.org/10.1111/j.1365-2958.1991.tb00779.x
  28. J. Biol. Chem. v.267 Membrane topology of the ArsB protein,the membrane subnit of an anion-translocating ATPase Wu,J.;L.S.Tisa;B.P.Rosen
  29. J. Biol. Chem. v.268 Metalloregulated expression of the ars operon Wu,J.;B.P.Rosen
  30. Mol. Microbiol. v.8 The arsD gene encoded a second trans-acting regulatory protein of the plasmid-encoded arsenical resistance operon Wu,J.;B.P.Rosen https://doi.org/10.1111/j.1365-2958.1993.tb01605.x
  31. J. Biol. Chem. v.271 The chromosomal arsR gene of Escherichia coli encodes a trans-acting metalloregulatory protein Xu,C.;W.Shi;B.P.Rosen https://doi.org/10.1074/jbc.271.5.2427
  32. J. Biol. Chem. v.272 Dimerization is essential for DNA binding and repression by the ArsR metalloregulatory protein of Escherichia coli Xu,C.;B.P.Rosen https://doi.org/10.1074/jbc.272.25.15734
  33. J. Microbiol. Biotechnol. v.12 Plasmid-mediated arsenical and antimonial resistance determinants(ars)of Psdudomonas sp.KM20 Yoon,K.P.
  34. J. Microbiol. Biotechnol. v.13 Construction and characterization of multiple heavy metal-resistant phenol-degrading Pseudomonads strains Yoon,K.P.