• Journal of Acupuncture Research
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• 제17권1호
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• pp.221-250
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• 2000

In order to investigate experimentally that the effect of aqua-acupuncture solution of Paeonia lactiflora on arthritis of mice induced by collagenII, the author performed several experimental items : that is increase, paw thickness, DTH, weight of spleen, hematological change, expression of $CD4^+$, $CD8^+$, $CD19^+$, gene expression of IL-1, IL-6, IL-12, IFN-${\gamma}$, TNF, proliferation of synovial cells and cytotoxicity. The results were obtained as follows; 1. Inhibitory effects of aqua-acupuncture solution of Paeonia lactiflora on arthritis induced by collgenII. 1) In incidence, paw edema, AI and DTH were inhibited as compared with control group. 2) The splenic weight was increased and the number of leukocytes was decreased as compared with control group. 3) The number of $CD4^+$, $CD8^+$ activated cells and surface-receptor expression were increased as compared with control group. 4) In hematological change, total protein, creatinine and LDH were decreased significantiy as compared with control group. 2. FACS analysis on normal BABL/c of spenic cells treated with aqua-acupuncture solution of Paeonia lactiflora. 1) Aqua-acupuncture solution of Paeonia lactiflora activated adhesive splenic cells of mice morphologically in vitro. 2) Aqua-acupuncture solution of Paeonia dactiflora enhanced the gene expression of IL-12 and also enhanced that of interferon-${\gamma}$ remarkably. 3) Aqua-acupuncture solution of Paeonia lactiflora reduced the number of $CD4^+$, $CD8^+$, $CD19^+$ activated cells and their surface-receptor expression as compared with control group. 3. Effects of aqua-acupuncture solution of Paeonia lactiflora on human synovial cells. 1) In cytotoxicity against synovial cells, aqua-acupuncture solution of Paeonia lactiflora didn't show cytotoxicity at concentration of $10-100{\mu}g/m{\ell}$ but showed significantly at concentration of $200-400{\mu}g/m{\ell}$ as compared with control group. 2) Aqua-acupuncture solution of Paeonia lactiflora reduced the gene expression of IL-6, IL-$1{\beta}$ and TNF-${\alpha}$. 3) Aqua-acupuncture solution of Paeonia lactiflora inhibited proliferation of synovial cells at concentration of 100 and $200{\mu}g/m{\ell}$.

• 대한한의학회지
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• 제18권1호
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• pp.218-237
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• 1997

In order to investigate the antihypertentive effects and its mechanism of the Siegesbeckia pubescens aqua-acupuncture treatment, experiments were performed on immediate and continuous antihypertensive effects, vasodilatation-autonomic nerve block, diuretic activity, and angiotensin converting enzyme inhibitory activity. The results were as follows; 1. Acupuncture treatment group showed significantly immediate antihypertensive effects in 4 hours after treatment. Normal saline aqua-acupuncture treatment group showed significantly immediate antihypertensive effects in 2, 4 and 6 hours after treatment. Siegesbeckia pubescens aqua-acupuncture treatment group showed significantly immediate antihypertensive effects in 30 minutes, 1, 2, 4 and 6 hours after treatment. 2. Siegesbeckia pubescens aqua-acupuncture treatment group showed significantly continuous antihypertensive effects in 6, 8, 9 and 10 days after treatment. 3. Siegesbeckia pubescens aqua-acupuncture extract solution showed significantly vasodilatatory and sympathetic nerve block effects with concentration of $10^{-6},\;10^{-5},\;10^{-4},\;10^{-3}g/m{\ell}$. 4. Oral administration group of the Siegesbeckia pubescens aqua-acupuncture extract solution didn't show no significantly diuretic effects. 5. Siegesbeckia pubescens aqua-acupuncture extract solution showed 28.8% angiotensin converting enzyme inhibitory activity.

• Journal of Acupuncture Research
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• 제15권2호
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• pp.357-367
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• 1998

This experiment was carried out to study on the acute toxicity of Thesium Chinese Turczaninow solution for aqua-acupuncture. Test material was manufactured by 'Water Alcohol method'. Mice and rats were used for this test. According to this experimental results, there were not observed any acute toxicity with Thesium Chinese Turczaninow solution for aqua-acupuncture. So Thesium Chinese Turczaninow solition for aqua-acupuncture is considered a have level of cliniccal safety. It is expected to be clinically helpful, and it is considered that continuous study is necessary.

• 한방안이비인후피부과학회지
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• 제16권2호
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• pp.212-220
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• 2003

Objectives: The purpose of this study is to evaluate the efficacy of Hominis Placenta Aqua-acupuncture Solution in patients of melasma. Methods: The treatment was performed twice weekly in 15 volunteer patients for 8 weeks by Hominis Placenta Aqua-acupuncture Solution. We used Melasma Area and Severity Index(MASI) to evaluate the severity of melasma. Results: MASI score was decreased from A to B and the significant clinical improvement was proved compared to before treatment. Conclusions: On the basis of this study, Hominis Placenta Aqua-acupuncture Solution is an effective treatment of melasma.

• Journal of Acupuncture Research
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• 제17권1호
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• pp.67-74
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• 2000

Hominis Placenta has been used as a medicinal stuff for a long time in Oriental Medicine. And further, aqua-acupuncture solution of Hominis Placenta has produced in South Korea recently. Bibliographical study was carried on to investigate the nature of Hominis Placenta and possible indications by aqua-acupuncture as well. The nature of Hominis Placenta is warm and its taste sweet-salty. It is non-poisonous. It has correspondence to the meridians of lung, liver and kidney. Hominis Placenta is mainly effective on recuperation of infirmity and the disease related to infirmity. Aqua-acupuncture solution of Hominis Placenta can be applied to the disease as chronic hepatitis, liver cirrhosis, asthma, pulmonary tuberculosis, sterility, insufficient lactation, degenerative change, neurasthenia and cerebrovascular disease.

• Korean Journal of Acupuncture
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• 제17권1호
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• pp.11-17
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• 2000

금은화, 당귀, 감두를 이용하여 조제한 각각의 약침액을 이용하여 암예방효과를 살펴 보았다. 발암물질을 무독화시키는 QR 생성 유도를 생쥐의 간암세포인 Hepa1c1c7과 측정하였으며, 그 결과 금은화 약침액, 당귀 약침액, 감두약침액을 처리한 Hepa1c1c7에서 QR생성이 유도되었으며 GSH 생성을 살펴본 결과 Hepa1c1c7 세포에서 실험에 이용된 모든 생약 약침액에 의한 GSH 생성이 증가하였고, GST 생성 또한 증가하였다.

• 생명과학회지
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• 제9권6호
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• pp.677-683
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• 1999

Gamdutang aqua-acupuncture solution (GAS), Gamdutang water-extracted solution (GWS) and Dae-Gamdutang aqua-acupuncture solution (DGAS) were prepared and tested for antitumor activities. It was shown to possess considerable toxicity toward various tumor cell lines. Concentration of 5 $\times$ and 10$\times$ of GAS resulted in more than 70% inhibition of growth in Ehrlich ascites tumor cells (EATC), Hepa1c1c7 and A549. GAS at concentrations of 5$\times$ and 10$\times$ revealed more than 60% inhibition in HeLa. GWS showed more than 50% inhibition of growth with EATC and HeLa at concentrations of 5$\times$ and 10$\times$, respectively. Toxicity assay with GWS in Hepa1c1c7 and A549 revealed that more than 80% inhibition of growth at the concentration of 5$\times$ and 10$\times$. In morphological study, the number of cells were decreased, and the shape of cells was round-form in EATC, Hepa1c1c7, A549, HeLa with GAS.

• 생명과학회지
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• 제11권4호
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• pp.335-339
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• 2001

Cancer chemoprevention refers to the use of natural or synthetic substances to prevent initiational and promotional events that occur during the process of carcinogenesis. Thesium Chinese Turczaninow aqua-acupuncture solution (TCTAS) and Astragli Radix aqua-acupuncture solution (ARAS) were tested as the cancer chemopreventive agents using biochemical markers of carcinogenesis. The effects on the inhibition of phorbol 12- myristate 13-acetate(TPA)-induced free radical formation in HL-60 cells and the inhibition of polyamine metabolism were measured. There is significant inhibition of TAP-induced free radical formation in human leukemic cells with cells with ARAS. Proliferation of Acanthamoeba castellanii was inhibited by TCTAS and ARAS. TCTAS and ARAS positive in these assays may inhibit the carcinogenesis process and is considered promising cancer-preventing agents.

• Journal of Acupuncture Research
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• 제15권2호
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• pp.237-253
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• 1998

These studies were carried out to observe the effect of Aqua-acupuncture with Woolgum extract on the recovery from liver injury of rats. The liver injury of rats induced with 0.5ml/200g carbon tetrachloride. The Aqua-acupuncture with Woolgum extract solution inserted into corresponding locus of the Kansu(BL18.) in Rats. Experimental group was divided into 4 groups: Normal group, $CCI_4$-intoxicated group, Saline treated group after $CCI_4$-intoxicated and Woolgum aqua-acupuncture treated group after $CCI_4$-intoxicated. Biochemical assays for each serum enzyme activity of GOT, GPT, LDH, total protein, triglyceride, albumin, WBC, neutrophils, lymphocyte were performed. The results were as follows : 1. GOT was decreased with statistical significance in the Woolgum aqua-acupuncture treated group after $CCI_4$-intoxicated. In GOT activity, the Woolgum aqua-acupuncture treated group after $CCI_4$-intoxicated showed higher level significant compared with the saline aqua-acupuncture treated group after $CCI_4$-intoxicated. 2. GPT was decreased with statistical significance in the Woolgum aqua-acupuncture treated after $CCI_4$-intoxicated. In GOT activity, the Woolgum aqua-acupuncture treated group after $CCI_4$-intoxicated showed higher level significant compared with the saline aqua-acupuncture treated group after $CCI_4$-intoxicated. 3. LDH was decreased with statistical significance in the Woolgum aqua-acupuncture treated group after $CCI_4$-intoxicated. 4. Total protein increased with statistical significance in the Woolgum aqua-acupuncture treated group after $CCI_4$-intoxicated. 5. Albumin was increased with statistical significance in the Woolgum aqua-acupuncture treated group after $CCI_4$-intoxicated. The saline aqua-acupuncture treated group after $CCI_4$-intoxicated was not recognized as compared with $CCI_4$-intoxicated group. 6. Triglyceride was increased with statistical significance in the Woolgum aqua-acupuncture treated group after $CCI_4$-intoxicated. 7. WBC was decreased with statistical significance in the Woolgum aqua-acupuncture treated group after $CCI_4$-intoxicated. In WBC count, the Woolgum aqua-acupuncture higher level significant compared with the saline aqua-acupuncture treated group after $CCI_4$-intoxicated. 8. Lymphocyte was decreased with statistical significance in the Woolgum aqua-acupuncture treated group after $CCI_4$-intoxicated. 9. Neutrophils was increased with statistical significance in the Woolgum aqua-acupuncture treated group after $CCI_4$-intoxicated. The saline aqua-acupuncture treated group after $CCI_4$-intoxicated was not recognized as compared with $CCI_4$-intoxicated group. From above results, it is suggested that the Woolgum aqua-acupuncture had significant effects on the injury induced by $CCI_4$.

• 대한한의학회지
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• 제17권2호
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• pp.168-177
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• 1996

In order to study the effect of aqua-acupuncture with Cervi Cornu (C.C.) extract solution manufactured by water-alcohol method on the lipid metabolism and osteoporosis, C.C. aqua-acupuncture was carried out every two days for 60 days on the corresponding bilateral loci of Shinsu (BL23), Taejo (BL11), and Hyonjong (GB39) from the 10th day after the ovariectomy for induced osteoporosis and thereafter the level of serum triglyceride, LDL, Ca, phosphorus and osteocalcin, as well as urine hydroxyproline were measured in the ovariectomized rats. The rats being studied were divided into a normal group (sham operation group), a control group (ovariectomized group), and a C.C. aqua-acupuncture group (group with C.C. aqua-acupunctured to the points of Shinsu, Taejo, and Hyonjong every two days for 60 days from the 10th day after the ovariectomy). The following results were obtained: 1. The serum triglyceride indicated an inhibited increase with statistical significance in the C.C. aqua-acupuncture group compared with the control group. 2. The serum LDL indicated an inhibited increase without any statistical significance in the C.C. aqua-acupuncture group compared with the control group. 3. The serum Ca showed an inhibited decrease without any statistical significance in the C.C. aqua-acupuncture group compared with the control group. 4. The serum phosphorus showed an inhibited decrease with statistical significance in the C.C. aqua-acupuncture group compared with the control group. 5. The serum osteocalcin indicated an inhibited decrease without any statistical significance in the C.C. aqua-acupuncture group compared with the control group. 6. The urine hydroxyproline showed an inhibited increase with statistical significance in the C.C. aqua-acupuncture group compared with the control group.