• KOREAN JOURNAL OF CROP SCIENCE
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• v.22 no.1
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• pp.41-44
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• 1977

Production of haploids in vitro anther culture of Nicotiana tabacum L. was oriented in a large number on a chemically defined culture medium. The haploids were screened for T.M.V. resistance and the segregating ratio in F$_1$ were in good agreement with the expected ratio.

• Proceedings of the Korean Society of Crop Science Conference
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• 1989.02a
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• pp.30-31
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• 1989

• Korean Journal of Plant Tissue Culture
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• v.27 no.4
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• pp.353-358
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• 2000

• Proceedings of the Korean Society of Crop Science Conference
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• 1990.10a
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• pp.56-57
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• 1990

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.3
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• pp.271-276
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• 1985

Effects of media and its components on callus induction and plant regeneration were studied to increase the cultural efficiency in rice anther culture. The N$_{6}$ basic medium gave better results in callus induction than those of MS or Miller. The medium used for callus induction affects the plant regeneration. The frequency of plant regeneration from callus grown on Miller basic medium was lower than those of N$_{6}$ or MS. Most of calli derived from anthers, above 90%, were induced from 20 days to 40 days after anther inoculation. The cultural efficiency of modified N$_{6}$ basic medium which was composed of 31.5mM KNO$_3$ and 1.75mM(NH$_4$)$_2$SO$_4$ as nitrogen sources was higher than those of N$_{6}$ basic medium. Combination of NAA and Kinetin showed better results than that of 2, 4-D only in cultural efficiency. Effect of DL-alanine on callus induction in Indica variety, IR40, showed better response in the anthers pretreated for 6 days at 12$^{\circ}C$.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.3
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• pp.207-210
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• 2000

We investigated the structure of floral organs and possibility of seed-set to breed a variety in ginger Zingiber officinale Rosc. Floral bud was formed from collected domestic Seosan var, and foregin Thailand var, the number of florets per bud were 8 and 10 in Seosan and Thailand var, respectively, Flowering time ranged from 18 to 25 August irregularly at 4-5 pm. The flower has the long styled with fiber hairs on top of stigma and connected-two anthers. Pollens were mixed of circular and ellips shape and its extine was two layer structure. Callus formation from anther explants was effective with compact and embryogenic on N$_{6}$ medium supplemented 2 mg/l of NAA(NCM). Plant regeneration was on the MS medium with BA of 1-2 mg/l from 40 days old callus after transferred callus medium.m.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.1
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• pp.74-78
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• 1991

This experiment was conducted to know the effects of mutagen treatments on callus induction, plant regeneration and their ploidy in the anther culture of wheat. The winter wheat cultivars, 'apos;and 'apos;Wonkwang'apos;, were treated at the mid or late-uninucreate stage under 4 different doses (100, 200, 500 and 1,000 rad.) of X-ray and 3 different levels(0.1, 0.2 and 0.3 mole) of Ethyl Methane Sulphonate. The anthers treated were set on the C$\_$17/medium for callus induction, and callus induced was transfered to 1/2 MS medium for plant regeneration. The mutagen treatments inhibited the callus induction but increased the plant regeneration in the callus which were induced from the anther set on the medium for the long time of 60 to 80 days. Also, the chromosome number to the regenerated plant varied largely by increasing of haploid plants(n=3x=21) and by occurring of aneuploidy having n=20 and n=22 of chromosome number.aried largely by increasing of haploid plants(n=3x=21) and by occurring of aneuploidy having n=20 and n=22 of chromosome number.

• Journal of the Korean Society of Tobacco Science
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• v.21 no.1
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• pp.82-88
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• 1999

The present study was conducted to compare the relative efficiency of two different haploid breeding methods in tobacco varietal development. A single FI hybrid plant from cross of two flue-cured cultivars of Nicotiana tabacum L., Bright Yellow4(BY4) and NC95, was used to develop the 30 anther derived dihaploid (ADH) lines and maternally-derived dihaploid (MDH) lines utilizing Nicotiana africana, respectively. As compared with mid-parent, mean of ADH lines showed increase in number of leaves, delay in days to flower and narrow in width of leaf. However, no significant differences in the other investigated characters were recognized. MDH lines also showed narrow width of leaf, while no significant differences in the other characters were observed. The variations of the investigated characters were generally greater in ADH than MDH lines. MDH lines had higher height of plant and shorter days to flower than ADH lines, while the other characters did not show remarkable differences. The degree of heritability for each of the characters observed between ADH and MDH was almost the same. The characters showing high heritability value were height of plant, number of leaves, days to flower, and yield, while those showing relatively low value were length of leaf, width of leaf, and total alkaloid content. Predicted gains from selection for increased yield were calculated for both populations(ADH, MDH) and correlated responses associated with selection for yield were estimated. Height of plant, width of leaf, days to flower, and concentration of reduceing sugar would be expected to improve with selection for yield much faster in the MDH population than in the ADH.

• Korean Journal of Plant Tissue Culture
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• v.26 no.1
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• pp.49-52
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• 1999

The influence of genotype and ecotype on the anther culture efficiency using hybrid of hot pepper (Capsicum annuum L.) was investigated. Anther culture efficiency was differently dependent on the genotype of parents. In the efficiency of embryo production, the cross combination using female parents with high embryo inducing ability was higher than those with low embryo inducing ability. It was shown that genotype and cytoplasm has effect on embryo production. Also the embryogenic ability was different according to ecotype of cross lines. The frequency of embryo production were the highest in Local variety $\times$ pimento cross combinations with 17.8~46.1 and the lowest in Pimento $\times$ Local variety cross combinations with 5.4~8.5%. Embryo inducing frequency was the middle value with 10.25~23.1% in Local variety $\times$ Tropical variety, Tropical variety $\times$ Local variety, Tropical variety $\times$ Pimento, and Pimento $\times$ Tropical variety cross combinations.

• Korean Journal of Plant Tissue Culture
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• v.27 no.2
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• pp.83-87
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• 2000

To investigate the effect of growth regulators and antioxidant in anther floating cultures of rice, anthers were cultured in liquid media supplemented with different growth regulators, and the effect of antioxidant mixture : (Sigma Chemical Co.) on callus induction and plant regeneration were investigated. N6 medium with 1 mg/L 2,4-D and 1 mg/L kinetin was the best for rice anther floating cultures, which showed 48.5% of callus induction and 6.8% of green plant regeneration. The callus induction was not affected by antioxidant mixture in liquid medium, and antioxidant mixture (250 mg/L) was effective for the reduction of brownish callus and improvement of plant regeneration Antioxidant mixture showed better effectiveness when it was supplemented to both media for callus induction and plant regeneration.