• 한국축산식품학회지
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• 제28권1호
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• pp.59-62
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• 2008

본 연구는 우유내 잔류물질 검사에 신속검출법으로 많이 사용되는 Delvo test가 산양유의 잔류물질 검사에도 적합한지 확인하기 위해 수행되었다. 8개 산양유목장 원유를 7회에 걸쳐 총 56개 시료를 Delvo test, Eclipse100, 그리고 Parallux로 분석하였다. 면역화학적 방법인 파라룩스에서는 양성 시료가 전혀 검출되지 않았으나 Delvo test에서는 37.5%의 양성율을 나타내었고 같은 미생물적 검출법인 Eclipse100에서는 3.6%의 양성율을 나타내었다. Delvo test는 국립수의과학검역원에서 발간한 원유검사표준화요령에 미생물학적 잔류물질 신속검출법으로 기재되어 있고, 축산물가공처리법상 "원유"의 정의가 '판매 또는 판매를 위한 처리 가공을 목적으로 하는 착유상태의 우유와 양유를 말한다'는 점을 고려할 때 Delvo test를 원유검사표준화요령에서 제외시키거나 Delvo test가 산양유의 잔류물질 신속검출법으로 적합하지 않다는 근거가 필요할 것으로 생각된다.

• Asian-Australasian Journal of Animal Sciences
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• 제23권4호
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• pp.425-429
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• 2010

To develop an efficient DNA typing system for Chinese Holstein cattle, 17 microsatellites, which were amplified in four fluorescent multiplex reactions and genotyped by two capillary electrophoresis injections, were evaluated for parentage verification and identity test. These markers were highly polymorphic with a mean of 8.35 alleles per locus and an average expected heterozygosity of 0.711 in 371 individuals. Parentage exclusion probability with only one sampled parent was approximately 0.999. Parentage exclusion probability when another parent' genotype was known was over 0.99999. Overall probability of identity, i.e. the probability that two animals share a common genotype by chance, was $1.52{\times}10^{-16}$. In a test case of parentage assignment, the 17 loci assigned 31 out of 33 cows to the pedigree sires with 95% confidence, while 2 cows were excluded from the paternity relationship with candidate sires. The results demonstrated the high efficacy of the 17 markers in parentage analysis and individual identification for Chinese Holstein cattle.

• 한국임상수의학회지
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• 제30권6호
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• pp.449-452
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• 2013

본 연구는 제주산마의 hill test 및 운동 강도별 심폐지구력 측정으로 승용능력 평가를 위한 기초 자료로 활용하기 위해 수행하였다. 분석에 이용된 제주산마 9두는 경사도 30도 내외의 경사로 800 m를 주행하는 hill test를 실시하였으며, 이 중 6 두는 운동 강도별(moderate, heavy, very heavy) 심폐지구력을 측정하였다. Hill test 주행시간은 평균 205 초였으며, 개체간 편차는 큰 것으로 확인 되었고, moderate exercise와 높은 상관관계를 보였다. 운동 강도별 평균 심박수는 유의적인 차이가 없었으나, 개체간 평균 심박수는 차이가 큰 것으로 나타났다. 회복 심박수는 heavy exercise와 very heavy exercise에서 높은 상관관계를 확인할 수 있었다. 본 실험결과, 운동 강도별 평균 심박수 차이는 확인 할 수 없었으나, 개체간의 차이는 큰 것으로 확인되었다.

• 한국수정란이식학회지
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• 제28권3호
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• pp.223-227
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• 2013

The objective of the study is to assess temperament of a horse based on general temperament test by a questionnaire survey. Five test criteria were identified: gentleness, patience, aggressiveness, sensitivity, and friendliness, each on a 5-point scale. 114 horses bred at the National Institute of Animal Science (NIAS) in 2010, 2011 and 2012. The horses recorded scores of 3.6~3.9 for gentleness, 3.1~3.6 for patience, 3.4~4.0 for aggressiveness, 2.8~3.2 for sensitivity, and 3.4~3.8 for friendliness, the overall score for sensitivity the lowest. Horses born in 2012 scored lower than the rest in all five areas at a statistically significant level (P<0.05). By gender, the colts scored higher than the fillies in all five areas, but the discrepancy was not statistically significant. Factor analysis yielded only one factor, and the Cronbach's ${\alpha}$ value was 0.980 for standardization of Factor 1, indicating a high reliability of internal consistency. The correlation coefficients among the test criteria ranged between 0.85 and 0.91 (P<0.01). The assessment criteria used in this study are expected to provide a useful basis designing a temperament test horses.

• Toxicological Research
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• 제13권3호
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• pp.203-208
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• 1997

The antigenic potential of M3S tumor necrosis factor-$\alpha$(M3S TNF), which is a mutated form of TNF(TNF mutein) designed to reduce adverse effects of wild type human TNF, was investigated in the present study. The antigenicity of M3S TNF was examined by conducting active systemic anaphylaxis (ASA) test in guinea pigs, heterologous(mouse-rat) passive cutaneous anaphylaxis(PCA) test and passive hemagglutination(PHA) test. The experimental animals were divided into low, medium, high and the highest dose groups and the groups with or without immunoadjuvant, sensitized according to the appropriate schedule and challenged. In ASA test, when challenged with 120 $\mu\textrm{g}$ /animal, moderate to severe positive anaphylactic responses were observed in groups sensitized with 12 $\mu\textrm{g}$ /animal, 120 $\mu\textrm{g}$ /animal and 120 $\mu\textrm{g}$ /animal+Freund's complete adjuvant. In PCA test, positive responses were observed in the group sensitized with the highest dose emulsified with an alum(12 $\mu\textrm{g}$ /animal+alum). In PHA test, positive responses were observed in the group sensitized with 3 $\mu\textrm{g}$ /animal emulsified with an alum. All the other groups in each experiment showed negative responses. Based on these results, M3S TNF is considered to have some antigenic potential.

• 대한안전경영과학회지
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• 제12권4호
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• pp.73-80
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• 2010

The purpose of this study was to investigate the actual conditions of radiation safety supervision in animal clinics using inspection standard of X-ray generator for diagnosis. The surveys for inspection standard system, equipment condition, and safety supervision were carried out in 18 animal clinics randomly. The inspection standard included reproducibility of dose exposure, kVp, mAs, collimator accuracy test, collimator luminance test, X-ray view box luminance test, grounding system equipment test and external leakage current test. The surveys of equipment condition and safety supervision used one-on-one interview with 5 points measurement. As a result, 44.44% of reproducibility of dose exposure was proper, 81.25% of kVp test was good, and 100% of mAs test was appropriate. Also, 66.66% of collimator accuracy test was proper, 61.11% of collimator luminance test was good, 53.13% of X-ray view box luminance test was suitable. In addition, only 5.55% of grounding system equipment and ground resistance was proper, 63.64% of external leakage current test was appropriate in grounding system equipment test. The 100mA electric capacity of X-ray generator for diagnosis was popular with 44.44%, and its 55.56% was purchased used equipment. Monthly average of less than 50 times (61.11%) was top frequency in use, and no animal clinic had a thermo-luminescence dosimeter(TLD). The 16 animal clinics with radiation safety zone and 2 without radiation safety zone were appeared.

• Asian-Australasian Journal of Animal Sciences
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• 제26권9호
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• pp.1229-1236
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• 2013

India possesses a total buffalo population of 105 million out of which 26.1% inhabit Uttar Pradesh. The buffalo of Uttar Pradesh are described as nondescript or local buffaloes. Currently, there is no report about the genetic diversity, phylogenetic relationship and matrilineal genetic structure of these buffaloes. To determine the origin and genetic diversity of UP buffaloes, we sequenced and analysed the mitochondrial DNA D-loop sequences in 259 samples from entire Uttar Pradesh. One hundred nine haplotypes were identified in UP buffaloes that were defined by 96 polymorphic sites. We implemented neutrality tests to assess signatures of recent historical demographic events like Tajima's D test and Fu's Fs test. The phylogenetic studies revealed that there was no geographic differentiation and UP buffaloes had a single maternal lineage while buffaloes of Eastern UP were distinctive from rest of the UP buffaloes.

• 한국수정란이식학회지
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• 제28권3호
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• pp.207-213
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• 2013

This study was carried out to examine a molecular marker system for parentage test in Jeju Black cattle (JBC). Based on the preliminarily studies, we finally selected for construction of a novel genetic marker system for molecular traceability, identity test, breed certification, and parentage test in JBC and its related industrial populations. The genetic marker system had eight MS markers, five indel markers, and two single nucleotide polymorphisms (SNPs; g.G299T and g.del310G) within MC1R gene which is critical to verify the breed specific genotypes for coat color of JBC differing from those of exotic black cattle breeds such as Holstein and Angus. The results showed lower level of a combined non-exclusion probability for second parent (NE-P2) of $4.1202{\times}10^{-4}$ than those previously recommended by International Society of Animal Genetics (ISAG) of $5.000{\times}10^{-4}$ for parentage, and a combined non-exclusion probability for sib identity (NE-SI) of $2.679{\times}10^{-5}$. Parentage analysis has been successfully identified the JBC offspring in the indigenous population and cattle farms used the certified AI semens for production using the JBC-derived offspring for commercial beef. This combined molecular marker system will be helpful to supply genetic information for parentage test and traceability and to develop the molecular breeding system for improvement of animal productivity in JBC population.

• 대한수의학회지
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• 제61권2호
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• pp.19.1-19.7
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• 2021

Feline panleukopenia virus (FPV) causes leukopenia and severe hemorrhagic diarrhea, killing 50% of naturally infected cats. Although intact FPV can serve as an antigen in the hemagglutination inhibition (HI) test, an accidental laboratory-mediated infection is concern. A non-infectious diagnostic reagent is required for the HI test. Here, we expressed the viral protein 2 (VP2) gene of the FPV strain currently prevalent in South Korea in a baculovirus expression system; VP2 protein was identified by an indirect immunofluorescence assay, electron microscopy (EM), Western blotting (WB), and a hemagglutination assay (HA). EM showed that the recombinant VP2 protein self-assembled to form virus-like particles. WB revealed that the recombinant VP2 was 65 kDa in size. The HA activity of the recombinant VP2 protein was very high at 1:2¹⁵. A total of 143 cat serum samples were tested using FPV (HI-FPV test) and the recombinant VP2 protein (HI-VP2 test) as HI antigens. The sensitivity, specificity, and accuracy of the HI-VP2 test were 99.3%, 88.9%, and 99.3%, respectively, compared to the HI-FPV test. The HI-VP2 and HI-FPV results correlated significantly (r = 0.978). Thus, recombinant VP2 can substitute for intact FPV as the serological diagnostic reagent of the HI test for FPV.

• 대한안전경영과학회:학술대회논문집
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• 대한안전경영과학회 2010년도 춘계학술대회
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• pp.231-237
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• 2010

The purpose of this study was to investigate the actual conditions of radiation safety supervision in animal clinics using quality assurance (QA) and quality control (QC) of diagnostic X-ray units. The surveys for QA/QC, equipment condition, and safety supervision were carried out in 18 animal clinics randomly. The QA/QC included reproducibility of dose exposure, kVp, mAs, collimator accuracy test, collimator luminance test, X-ray view box luminance test, grounding system equipment test and external leakage current test. As a result, 44.44% of reproducibility of dose exposure was proper, 81. 25% of kVp test was good, and 100% of mAs test was appropriate. Also, 66.66% of collimator accuracy test was proper, 61.11% of collimator luminance test was good, 53.13% of X-ray view box luminance test was suitable. In addition, only 5.55% of grounding system equipment and ground resistance was proper, 63.64% of external leakage current test was appropriate in grounding system equipment test.